LOCUS       NM_206855              16283 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens QKI, KH domain containing RNA binding (QKI),
            transcript variant 4, mRNA.
ACCESSION   NM_206855 XM_037438
VERSION     NM_206855.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 16283)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16283)
  AUTHORS   Zakutansky,P.M., Ku,L., Zhang,G., Shi,L., Li,Y., Yao,B.,
            Bassell,G.J., Read,R.D. and Feng,Y.
  TITLE     Isoform balance of the long noncoding RNA NEAT1 is regulated by the
            RNA-binding protein QKI, governs the glioma transcriptome, and
            impacts cell migration
  JOURNAL   J Biol Chem 300 (8), 107595 (2024)
   PUBMED   39032650
  REMARK    GeneRIF: Isoform balance of the long noncoding RNA NEAT1 is
            regulated by the RNA-binding protein QKI, governs the glioma
            transcriptome, and impacts cell migration.
REFERENCE   3  (bases 1 to 16283)
  AUTHORS   Wu,W., Cai,Y., Jin,J., Chen,Z. and Teng,M.
  TITLE     Circular RNA Circ_0001322 inhibits gastric cancer progression by
            modulating the miR-1264/QKI axis and suppressing the Hedgehog
            pathway
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (5), 190-197 (2024)
   PUBMED   38814217
  REMARK    GeneRIF: Circular RNA Circ_0001322 inhibits gastric cancer
            progression by modulating the miR-1264/QKI axis and suppressing the
            Hedgehog pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 16283)
  AUTHORS   Wang,H.F., Zhou,X.F., Zhang,Q.M., Wu,J.Q., Hou,J.H., Xu,X.L.,
            Li,X.M. and Liu,Y.L.
  TITLE     Involvement of circRNA Regulators MBNL1 and QKI in the Progression
            of Esophageal Squamous Cell Carcinoma
  JOURNAL   Cancer Control 31, 10732748241257142 (2024)
   PUBMED   38769028
  REMARK    GeneRIF: Involvement of circRNA Regulators MBNL1 and QKI in the
            Progression of Esophageal Squamous Cell Carcinoma.
REFERENCE   5  (bases 1 to 16283)
  AUTHORS   Min,K.W., Jo,M.H., Song,M., Lee,J.W., Shim,M.J., Kim,K., Park,H.B.,
            Ha,S., Mun,H., Polash,A., Hafner,M., Cho,J.H., Kim,D., Jeong,J.H.,
            Ko,S., Hohng,S., Kang,S.U. and Yoon,J.H.
  TITLE     Mature microRNA-binding protein QKI promotes microRNA-mediated gene
            silencing
  JOURNAL   RNA Biol 21 (1), 1-15 (2024)
   PUBMED   38372062
  REMARK    GeneRIF: Mature microRNA-binding protein QKI promotes
            microRNA-mediated gene silencing.
REFERENCE   6  (bases 1 to 16283)
  AUTHORS   Wu,J.I., Reed,R.B., Grabowski,P.J. and Artzt,K.
  TITLE     Function of quaking in myelination: regulation of alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 99 (7), 4233-4238 (2002)
   PUBMED   11917126
REFERENCE   7  (bases 1 to 16283)
  AUTHORS   Li,Z.Z., Kondo,T., Murata,T., Ebersole,T.A., Nishi,T., Tada,K.,
            Ushio,Y., Yamamura,K. and Abe,K.
  TITLE     Expression of Hqk encoding a KH RNA binding protein is altered in
            human glioma
  JOURNAL   Jpn J Cancer Res 93 (2), 167-177 (2002)
   PUBMED   11856480
REFERENCE   8  (bases 1 to 16283)
  AUTHORS   Saccomanno,L., Loushin,C., Jan,E., Punkay,E., Artzt,K. and
            Goodwin,E.B.
  TITLE     The STAR protein QKI-6 is a translational repressor
  JOURNAL   Proc Natl Acad Sci U S A 96 (22), 12605-12610 (1999)
   PUBMED   10535969
  REMARK    GeneRIF: There are many isoforms of this RNA binding protein. QKI-6
            has been shown to be a translational repressor.
REFERENCE   9  (bases 1 to 16283)
  AUTHORS   Chen,T. and Richard,S.
  TITLE     Structure-function analysis of Qk1: a lethal point mutation in
            mouse quaking prevents homodimerization
  JOURNAL   Mol Cell Biol 18 (8), 4863-4871 (1998)
   PUBMED   9671495
REFERENCE   10 (bases 1 to 16283)
  AUTHORS   Ebersole,T.A., Chen,Q., Justice,M.J. and Artzt,K.
  TITLE     The quaking gene product necessary in embryogenesis and myelination
            combines features of RNA binding and signal transduction proteins
  JOURNAL   Nat Genet 12 (3), 260-265 (1996)
   PUBMED   8589716
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB067801.1, AF272349.1,
            AL031781.1 and AW131362.1.
            
            On May 31, 2019 this sequence version replaced NM_206855.2.
            
            Summary: The protein encoded by this gene is an RNA-binding protein
            that regulates pre-mRNA splicing, export of mRNAs from the nucleus,
            protein translation, and mRNA stability. The encoded protein is
            involved in myelinization and oligodendrocyte differentiation and
            may play a role in schizophrenia. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (4) differs in the 3' UTR and
            coding region compared to variant 1, which results in a frameshift.
            The resulting isoform (4; also known as HQK-7B and QKI-7b) is
            shorter and has a distinct C-terminus compared to isoform 1.
            Isoforms 2 and 4 are the same length but have different C-termini.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB067801.1, SRR1163657.313204.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               AB067801.1         76-479
            405-1410            AF272349.1         362-1367
            1411-7836           AB067801.1         1487-7912
            7837-16240          AL031781.1         4352-12755          c
            16241-16283         AW131362.1         21-63               c
FEATURES             Location/Qualifiers
     source          1..16283
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q26"
     gene            1..16283
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="QKI, KH domain containing RNA binding"
                     /db_xref="GeneID:9444"
                     /db_xref="HGNC:HGNC:21100"
                     /db_xref="MIM:609590"
     exon            1..618
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             477..1436
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="isoform 4 is encoded by transcript variant 4;
                     homolog of mouse quaking QKI (KH domain RNA binding
                     protein); RNA binding protein HQK; quaking homolog, KH
                     domain RNA binding; QKI/LOC100132735 fusion; KH
                     domain-containing RNA-binding protein QKI"
                     /codon_start=1
                     /product="KH domain-containing RNA-binding protein QKI
                     isoform 4"
                     /protein_id="NP_996737.1"
                     /db_xref="CCDS:CCDS43525.1"
                     /db_xref="GeneID:9444"
                     /db_xref="HGNC:HGNC:21100"
                     /db_xref="MIM:609590"
                     /translation="MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERL
                     LDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRI
                     LGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDA
                     QNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSL
                     AATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVP
                     PGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGKFFSPWG"
     exon            619..761
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            762..878
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1022
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1110
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..16283
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7811..7816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      7836
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="major polyA site"
     regulatory      11464..11469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      11487
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="major polyA site"
     regulatory      11543..11548
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      11555
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      14346..14351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      14367
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      16199..16204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: ATTAAA"
     polyA_site      16218
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      16259..16264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: ATTAAA"
     polyA_site      16283
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
ORIGIN      
        1 gcagtcggag cgcggcggca gcggcagagg cgccgcggcg gggaccagcc cagagagacc
       61 ccccgagccc gcggcacagg cggcggcggc gctgagcggg ctcgaccagc cgagcgagcg
      121 gcggccccgg ctcctcctcg tccggcgcca gcagcccgcg ccccgcgccc gcactcggcc
      181 agccgagacc cgcctcccgc ccgcccgccg gcctcgtgag ggacgcgccg agccgggcgg
      241 ccgagagcgg cggcggctgg gagcgcgtcg ggcccgggcg gaaagtgcct gcggggggcg
      301 ggcgagcgcg cggtgccggc cgccccgggg ctcggcgcgg gagccagagc gggagccggc
      361 gcggagcggg acgccgggtc ccgagcggcc cgcggccggg gctcgccccc gcccctccct
      421 cctctccggc ggcggcggcg gcggcggcgg gcggagtgag ctgcggagcc tggaatatgg
      481 tcggggaaat ggaaacgaag gagaagccga agcccacccc agattacctg atgcagctga
      541 tgaacgacaa gaagctcatg agcagcctgc ccaacttctg cgggatcttc aaccacctcg
      601 agcggctgct ggacgaagaa attagcagag tacggaaaga catgtacaat gacacattaa
      661 atggcagtac agagaaaagg agtgcagaat tgcctgatgc tgtgggacct attgttcagt
      721 tacaagagaa actttatgtg cctgtaaaag aatacccaga ttttaatttt gttgggagaa
      781 tccttggacc tagaggactt acagccaaac aacttgaagc agaaaccgga tgtaaaatca
      841 tggtccgagg caaaggctca atgagggata aaaaaaagga ggagcaaaat agaggcaagc
      901 ccaattggga gcatctaaat gaagatttac atgtactaat cactgtggaa gatgctcaga
      961 acagagcaga aatcaaattg aagagagcag ttgaagaagt gaagaaatta ttggtacctg
     1021 cagcagaagg agaagacagc ctgaagaaga tgcagctgat ggagcttgcg attctgaatg
     1081 gcacctacag agatgccaac attaaatcac cagcccttgc cttttctctt gcagcaacag
     1141 cccaggctgc tccaaggatc attactgggc ctgcgccggt tctcccacca gctgccctgc
     1201 gtactcctac gccagctggc cctaccataa tgcctttgat cagacaaata cagaccgctg
     1261 tcatgccaaa cggaactcct cacccaactg ctgcaatagt tcctccaggg cccgaagctg
     1321 gtttaatcta tacaccctat gagtacccct acacattggc accagctaca tcaatccttg
     1381 agtatcctat tgaacctagt ggtgtattag gtaagttctt ctccccatgg ggttaacaac
     1441 attctcttta taaatatttt tgctccctca gattttgaaa tgattttatc agttttagag
     1501 aggcaagaca agtttgcatt agggaagacc gaaaactaaa ttttgtttta tttcagcctc
     1561 tcataagggt tcccctttga aataattgca ccttaaattt attcagatcc actttggtaa
     1621 ctgaggttca gtttggttgg gtaaatctgt tgtgtacatt tcaacaaata cttttacatt
     1681 tgacattact gaggtcattc tgagttttag gtcccacccc attggcccgt cacctccatc
     1741 agctccactt ctcaaacttt atttgagatt tattttatct cacaggtttg tgtaattgtt
     1801 ggaaaatttt taaacttcta agtgaaagta gtctgagcgc tttataaaat tcatattatt
     1861 gtgtgtgttt tgtaatattt gcgatcactt aaaatatcag ataaatacag tcatgcatcg
     1921 cttaacaacg gggatacagt ctaagaattg ccttgtctgg cagtttcatc atttggtgta
     1981 catcatagag tgtacttaca cagacataga tgatatcgtt tactacacac ctaggctgtg
     2041 tggtatagcc attataatct tatgggacca catagtataa gtggtccgtt gttgaccaaa
     2101 atgttgttat gcaggcatga ctgtagttgt attcttaaga tttttcagtt cttgtgtttt
     2161 ggagtcctag tcatattaaa catgagagat actcttaatt tcagtagtac agtatggaat
     2221 agttaaataa atagcaaatc actgaattca gaatctcact taaggcagaa attaaaatca
     2281 taatataaac gcttggtttt acatgaacaa aaagtggtgt gctcgaaaaa gacattactg
     2341 atgccttttt tttatagagt ggattgaaat gccagtcatg cctgatattt cagcccattg
     2401 acttgctgga tgaaggacta gaatacagca gctgttataa cacgaccagt caatgtggaa
     2461 caaactgttt ctgtgcaacc cctttgtttt accagacaaa atttgaatac tttttttcct
     2521 gaattgtata tgaccttggt gctgcatgca tgctgttgac ttttaggact ttgatctttt
     2581 aaggtttttt ttccccagca ttaatattga tttataaaga tttgaaaatc tttaatgaac
     2641 tggagaacac taagatttaa actcgaaaat tcgttgttca agtaaagaaa gccatgatgc
     2701 tctgtatgtt atctgtgtgt gtgcatgcac tcaggtgccc tttgtttcat gaacaaatac
     2761 atttcattgt acatgttttc tgtttaaatc attgtataaa gtaattgcag gtcagaatta
     2821 taccacagaa ctgtttatga gaggcttgtg tctgttgcac atttcttgaa gcatttttaa
     2881 aataacatgt aacctgtaac cttgttgttt aagttttctt ttctattaat actctgtcct
     2941 gtggtcccgt gcatgctcct tttcccagaa ctcctctctg ctgcacccac agcatctgtt
     3001 cccgaggagt tatgactctt gacttcctgc agggctgggg ctcttagcca ccagctgctg
     3061 ttccagcact ttcagcgcaa gatctccctg attttgccac gtggaattgt acttgtatat
     3121 gattacctta tctaaaatga ataagaggtg atggaccagt ttactgctta gaaatagcaa
     3181 gaggcactgc agtaaaactt gtttctcatt gtaaagcttc atgtcttttg tttgttggaa
     3241 aatttttact tatagaaact taattattag actggtaaaa taaagaccaa aatatgcaga
     3301 tttctaattg gcattcataa ggtgaataat aataagtgcc caatgaaaaa atctattatg
     3361 gttaatttca tttcttgctt tgccacctaa gcagtaaaac atgatattga ccacttggag
     3421 aactcagaaa attattttaa atttctaagt tataataaat ttgcacacag ataacatgca
     3481 tgctatttat gtcacatctc acattaaatt attttaaaat aagcagtgcc cttcaaaaca
     3541 gatgcagaca tgtgtgttgg tagtagtgag gagattggta ttagcatcaa gtcttcattg
     3601 atgactaatt tttaattccc ttccttttat ctttaggtat ggctttccca acgaaaggct
     3661 aagaattcaa gaacggtctt aactgaaccc tcatcagatc tgaatttaac aaatgcttag
     3721 tctcagcagc ctccggggga aaaaagctta gcctagcagt cagtgactta cttgcacttt
     3781 ttgcacatag atataaagta aaattatgtt attaatttgg tttagtctgt aatattacac
     3841 agtaatggta atttataaag gagtgtatag tagtatactg actgctaagt gtactatact
     3901 gtttgcttta ctaatcacct aattcattgc agttcatact tattgactca gagtttaaaa
     3961 ccacaatctg taggctttaa gaattgcttt taaacctttt taagtgataa actcttgaag
     4021 tggtcttaag gttagcaaat aattgtctgt attaaacatg gcgttattta agtagtcctg
     4081 ctgcaagaaa ggcacttcag tgaatatgtg actagtaaag cttaaatatg tttggatcta
     4141 atagagctca tgaaatactg taacttgggg attgtaaatg aatggataca ataggttaag
     4201 gcccaagatg tttgaaatga atgcaatatt aatagatgca tatatacatg acatattgtg
     4261 gttaatttta aaactactgt gccttaacgt gtttcttaaa cttttgtagt aaatgaacat
     4321 ttgaaatcca ttttgataaa cctgctgtta atgttttttc cccccttgtg aatgttttct
     4381 aactttgtct tggtaattgc aatttaacta ggtgcggtgg ctactaaagt tcgaaggcac
     4441 gatatgcgtg tccatcctta ccaaaggatt gtgaccgcag accgaggtta gtttagttct
     4501 gcagttcttg tctataagaa atgcgttggg tgtccataaa atttgcaaca ccacacctga
     4561 tggaaaaatg taattgctta cctgcacaca gttttttttc cttttctaaa tttgtttgtg
     4621 atcattgaca gatttaaggg ttgggttttt tgttttggtt tggttttttc tcccttcata
     4681 ttttaactta ttaaatcttc tattattatg tactatgatt tattcctact aaaacataaa
     4741 ttagtcaatt tggggagcta ttaaattttg atgttgcctc agttattttc attaataact
     4801 tttgctaaaa tttacctttt aaaagttctg aaatttgggg ttggtattgc aaaatgaatt
     4861 gaacggatgg tgctttaatg agagaagtaa aggaatatat gcctcctgtg tacttttgga
     4921 gaaaactgaa gatttagttt ccatttttct ttataataaa atatttgagc cactgtataa
     4981 acatgagggt gcacatgtca agtacttagc taaaatcaac aaagtgagga ctgtgaagat
     5041 aactttcatg tagttggatt tttttatata aattagagcg tcaattccct tttattctta
     5101 cattctcttg ccaaaatgga tcctttgatt tttgtcattt gctgctaaaa tatattatct
     5161 gaaggtaaac tgtctcttga agttctgtgg tggtatgaga tccagctgtg tgaatataat
     5221 ttttgactaa cctgtgactg gaaaataagt cttcattttt ctcctttaca acttttataa
     5281 tttgtgtcct gtatatgccc ttggaccctt ttataaatta tttttgctta ttgaggaatg
     5341 gtattttcgg tttattgaca ttgaattgtt ttctaagttt ttccatcaca agataggaat
     5401 atcaagcatg gtagtaaaaa aggatttttt acaagtttct attatggcac atttgtttcc
     5461 tacaaattaa attgatcaaa gtctcaaata ttagtggagg aaaaagtgct ggatttgtac
     5521 ttcaattgta catcttctat gaatttatga aggacctacc tgctttatgt gattaccttg
     5581 agtactgatg ataaatacta gacacagctg ttgtccacat cagaaataac atgcctttcc
     5641 caaagacatt gttaatattt gtttagcata atccataaag aacgttgaag acttttaaac
     5701 taatgtattc atcactaaca gtgattgttc agtgaacaaa atagacccca gctccgtggt
     5761 cgtctatgag gatggtattc cttgtaagtg ttctgtatga gcacgtctca tagggatggg
     5821 gaagtttttt ggaaagtcac ttgagtttta aagaaactgg acaattccat gaataatttg
     5881 aggttttatt catattgcta atgtctacaa ttgttctaaa gagtatttta aagtagtttt
     5941 tttcccccag ctaatctttc cctatgtatg ccccctttta ggagatttct caccattttg
     6001 ctttaacaca ataattattg gcctacttaa tactccagtg ccttgagata gttggacata
     6061 ttttaggaag attttattgt atttggaaaa ttacgacaca ttccatgcat ggatcttttg
     6121 tagttacgtg ttttgattag tcctgtcact gtttttattt aaaatgcagt gtcagcaaac
     6181 cgaaatgctc atttcttttt tagtaacaga gtactcgtat acttatcact gtatgcactc
     6241 attgggtcta agggagatgt ctgaagtcct tcacttaaat tattttatga tactactgtt
     6301 ttctctattt ttgaggttaa aattatttta aaaaaatatt ttgaagagaa gtttattagt
     6361 ataagcattc atttaatgca aaaaaactca gcccttggac atataaccat gtaattctat
     6421 atgaacgttt agagtactac atggttgacc aatatctagt tctttactaa aagtaaactc
     6481 acctctttat atatatttca aaactgaatt tgaagcctat tagagaccaa ctgacctttt
     6541 agttgtaatg aactgtatgg tcttgcatgg tgacacacct gtgtaggagg ccaagacttg
     6601 gcagaggatg tagtctaggt gtcctggtat atataaatgg tcactcactt cgtttatata
     6661 ttccacactc agaaaagaag tcagagtttt ttctgagtaa tacaaaaacc atcacttaca
     6721 taatttgtat aagaaacttt aaacagtttt aatcaaaact atgagtaaca tcaaaaatgc
     6781 tttaagattc tatattttgg aatacctgtg aagtagtatg actacatcat gttaaatatt
     6841 tattttcttc tatgattttg gagcagcaat ttttatttta aataaaattc cacttttaag
     6901 aaattcaggg aagatttggt cacattgaag atacagtatt tttgtagtat ttataaactg
     6961 ttctaaatga tagactatag aaaacatttt tgtcatatga aggtaaatca gtccattatt
     7021 ttggatcatt taaactgaac attacacctt ctgggctttg atttatgaag tggcacagaa
     7081 tctaactttg cactgaatat cttttcattc acatctcctc tgccttagtc acaatggcaa
     7141 cagtacagaa aattctatca aacctcagaa tatagtagaa ataattaagc tgttgaatga
     7201 gtcttaaaaa ttatactact gttaagtgga ccaagtttgg tgaagcagaa tgtgacaaag
     7261 gttgattaag gaaggaacaa ctcaaggaca ttgggaatga taacttttcc acttgagaac
     7321 tactttatgt tttactgtaa tttttaaagt ttttttgtcc tttttgttat tttgcaaaag
     7381 aaaatagtat ttacaggtgg cttcttttaa aatataaaaa tataaagcag gaatgtatat
     7441 gaaatgtcag attttattgt atttgcagag tattagcttt gaaattgaat aaagaaagct
     7501 gtttgtagtt ttaaaatgcc ttattggtat caattagaaa tttcttctat tttttgatgt
     7561 acttagagct ttttgagtgt agaattttaa atggcaggat tttacagtgt ttacatgcaa
     7621 gtgcatttta taagtgttct atatgtgtaa aatagtattt tcaactggaa agtgttggct
     7681 agtgcaaaag gcctggccat tttctggttc ccatgatgtt gcctacactg ctagactaca
     7741 gtttagtatc gctttgtatc atgaggccaa gaaattccat gttgtttgta aatagaataa
     7801 ttgaaaaagc aataaacatt tattgaacaa aagaaacctt gtttggccca gagtttatgt
     7861 tgaaccaaat ggttcataga attttgaatt tctttagatt catttctgtt aatcatcagt
     7921 tattaataat cacatccatc attaatagtt gctttaatgc ttgcatctta gagaatacta
     7981 atgcttaata gcagtcagat actgataact gcactgactg tttaaaaatt ctgtatacca
     8041 aacctattca ttgttgtttt tcaatttcct tcattgactg ctactaacta ttagttgtta
     8101 gttgtatttt atttctattt tttcttgtta accagttttt tttttttttt tgtgggagtg
     8161 ggctcttctt catttgttca aatcatgata cgaaattgtc atgattttaa ttgtattctg
     8221 tagatggtgt caatcagttt gtcagaatta aacatgcttt tttttttatt agttgtgatt
     8281 agtttttcat gttgtcatta agccgtcact agctgaaact aatctcttct ctatcttttc
     8341 ttttcttttt tttgttttgt ttttttttgt ttctaaccac ccagccgcca ccggcaacta
     8401 acctatgacc ttctgacctc tgaactcttc acccaatgat gacctgacca tgcctgcctg
     8461 ctgatcagtt aactggtaat cgcctttgct tgcctgtcgt cagtgcagcg agctgaggca
     8521 cttgtccgtt cgtcttacca tctaaccaaa caaaagacaa agaaattgtt gtcctccaac
     8581 tcagcttttt tttttttttt ttcctgtttg ggtgaaagtg gttctagaaa ctgcactgaa
     8641 tagtagtaaa gcaataaggc ccaattcatc ccacagcact gatcatcttt taatatccca
     8701 ccctaagcga acggtaagaa ggcctctctt aagaagggga gacagatggt ccttaactac
     8761 tcaatgacag aggcagttac tgtgagagac ttctaggaat ctttttcttc tcatagcgaa
     8821 gtcaaagctc tctctgaatg tactgtgtga tgatgcatca tgcatgaacc ttcggtcagg
     8881 gatatcattg gtgaagtgat ttcaaaaagt attcaaaatt tgatatgctg tttagtcact
     8941 acagtgccct caaagggcag aagttgcagc cttttttata ttgcctgcca aaatttgaag
     9001 tattagaaga aagtgtgcca tgagagaaaa acttaaggag ttttgaaaag taatgcaaat
     9061 aacaaaactg caacactatt tttaaaaaga taaatatctg agttaaaatt actgaatctt
     9121 tattttacac ctaaaaaaat atgagaacaa ggtacatgca ttatgtgtca cattactggg
     9181 caaactgttc aagtattttt ttttaaacct ccctgtatag aaaaaaatca ttaaggatgt
     9241 aaaagccatg cttgcctatt tgctgtatac atgtaatgaa attgtagata aagtgtagtg
     9301 cattgaaaca aatgaacaaa aagtagatac ttttactata caagggtgct ggtgcagaaa
     9361 aaaatatata tatttttgga aatgtagcat tttatacttt caagtgttat aaaaaaaaag
     9421 aaaaagaaca aagaaaccct ttatttcatt aaatgatttt ttctggtggt tgtcaagaaa
     9481 caaaagacca aaagagcctt tttgtctttc ttttttattt tttaagtatt ggaataagtc
     9541 taaagaaaag aaagtgcctt attttccttc atggtcattc agtcaagtgt ctcataaaga
     9601 tcagctcctc cctcagaata caagttaatg catgttactc agtcgcccag tatgtgaaag
     9661 aagatatgag ggagacaaat gagttgatag tttgaattat aggatttttg ccacgttacc
     9721 ttgatgcaaa tttgccaaat ctgatactgt gaaaagattt gataactttt ctaatgcctg
     9781 actagtaagt tttaaaacat cattctttaa aagaatagtt taatactttt ggttattcca
     9841 atcttcaatg ttaacccaaa ggattctgag ataaaaatta tgtgtcatct aaacttcagt
     9901 ttacagacaa aattagagct ttgttttatt tggagaaggg ggatgcattc atttgatttt
     9961 gttttttgtt tttaattaat tgaatataat aggataccta gcaaatgttt tcacaaaagt
    10021 gaatctttgt gattttcatt ctagaaaaca atgttttaaa tcacccaaat ttaatcacat
    10081 gtctttatat cataccagat aactggtgta gtgtatcctt ttactgtttc aataacctct
    10141 gcctccccca ccccttaact ttaaaagaat gtgaaaaact agcatcatag tgcatataaa
    10201 tactcaacca catttcagct taagcttcta atttctcaag aagttgaaac agttagttat
    10261 gtagttgaag caatttgtgt gcaaagccta catctaaaca cttgagtgaa taatcattaa
    10321 ctgctcagta ccagacgtgg caaatctcaa gtgacagtgg accccccccc ccgcccagct
    10381 tatcaactcg tcccctcttc cacaccttcc cagcatcaca gtcagaggtc agcaggaagc
    10441 tgagtcatta ccttcttggt gacctccccc accatcttgt ctcacttgga aaccgttgtc
    10501 agtttattta ctatgcaata gacattcatt gttttgtatc cagctagctt tggtttaata
    10561 tgccactgct ttgtctttct gttacacata cagttttgat ttatttacaa tatatgctgt
    10621 gccattttga tttttatatt gtttggttgg ttgaataaat ttgcgacact caaacacttg
    10681 aggtgatagt tgttaaaaac aataccagta tttgatccag tttcatctca actatgttat
    10741 acctggacat tttagatgtt tatcaaaggt cttttatggg gaagaaagta aatgtatgaa
    10801 ataataaatg tgtgacattt atagggaatg ttggctctga acaaactttt gaaaacttgg
    10861 ttgacaaaat tttggatcaa ctgaaaaaag catgactttt gaaatctctg aatgccttgg
    10921 ttctcagtat tatcattctt tattgaattt atttcttatt aaaatatgta gtttttaaga
    10981 ctttttttct gacagtatta tgtaattttt tagcgtgggt agatgggagt gtcgcttgta
    11041 tgttatcgta cagctgacat gtatttttgt ctattcttta ttatcttagt ttcatgctat
    11101 gtatgtacca taaccaacct attgcctatg agaaacatgt aagataatgt atttacagcc
    11161 attgttacaa gtttataatg tatttttcta tcttgtttta tatgtatgtt atataacatt
    11221 caaaaagaat ttttttcttg attgagaaaa ggatacaaaa tgcaaaatcc acaattttga
    11281 taactgaaaa ttgccaattg ttttgcagta ctttattata ttgggtgtct tgtctttttt
    11341 gggcttttag ttagctaatg aatgaataca ttagacactt ttgggtttta gttgggattt
    11401 tacatagctt gcattttaat tctttggttc tttgctgttt ctattaaccc acagcattat
    11461 tttaataaat gttataatac caacctacta actctggact ttgtctgttt attaaccttt
    11521 atatgtttaa ttaaaataaa caaataaaga caaaagaatg taaagtcttg agttactgga
    11581 ctaaccctga agaacgtgac cacagataac atagtgtgac ttgtttttat gttgtttgtc
    11641 agctgacagt tttgcacact actgttaaaa tggctttggt tattctggtc ttttagcttg
    11701 ggggccgggg cgggtgtggg tgtaggtgca gtagagatgg gaacaaagta gcctttgttt
    11761 gaggcattct tctcttgcca cttgcctttc agcatctgca ttactaattc cacaccttat
    11821 ttttccttct ccaaaataat cactgtctgc tcacagcatc gagtaaaaag atacattgtc
    11881 atggtatata cctggagaaa ccagtttgat tctttgttga accatttctt ttaatttctg
    11941 taaataagta attttatggt cacattgtaa agcagagaaa ctgttgatat tttaccttta
    12001 tttagatttt tgttttaact tgaccttttt cctttggaaa gcgttgaact tgtttagcta
    12061 actactccta ttaggggagc ttaacttggg taaagtcaaa ggcccactgg ctgtttctca
    12121 tggtggcctg aagccccgct gctttccagt gaggaaaaga accctcagca cgttactgag
    12181 ctttcctcag gtattttttt ctctgtcacc tactttacaa atacagtcag actaaaacat
    12241 taaacaagaa gtttaagggg gaataacatt gggatttgaa aagttgtatg tgattaattt
    12301 cttgcatgca aatcaattta agatttctta aatatcttca tgcttttctt ggctggaact
    12361 tgggtaaatt aacctgtaat tatgaattat ttagtttaac tcaaggtgca tttttatttc
    12421 acagattgat acttgctttt ttctgtgaaa cagttctcct agataccaaa atcctcagga
    12481 tgaatttttg catttgtaaa taacaattta tctttgtaaa ttacatttta tttatttaga
    12541 gttctaaaat tgagggctga aatgaatgac atgtaataga agacaaaact gtaccaatac
    12601 gctgttaacc aatcagactg tccaggtatt ctcatttcat aaatatgtat acacacatat
    12661 aaatacacag agtagtgtta gctgatgtga gtgataacag cgaatcacca ttgagatggt
    12721 catttggatc atgtaaacaa accgtgtcat gtctctgatg ggagcggtgg gtagtgtact
    12781 gttaggtgga caggtctttt aacatgctac tatttgtaaa agtttccgtt gccataacag
    12841 ttcatgtagt agtctgtgaa aattcgaagc tgtctgtcaa gctgctaata tacaagttgt
    12901 catggtacca tgtaacttaa attgatgaat ttctatgcaa tttactattc tctgaagcct
    12961 atgaatgcag cctcatctca ttttcagtta agggtacttt atgtataatt atgtcattgc
    13021 tgccttttat gtaggaaatg taaaaaagtt ttaaaaggcg aaaataacat tacagatttc
    13081 atttttatac aggttacaat attaaacatg aaactaccaa attccaagaa tagtaaaatt
    13141 ctagagtcag ttgattgatt gaaactattt gacaaaacag ttgaaagctg agctttcagt
    13201 caagaagttg gtgaaattaa tgtccttaaa cttttccttt aggcaaaatg tcagggaaaa
    13261 cttaacatga aagtagttac tcccatacgc accagtgcag tagctccagg tgtaagaggt
    13321 cacgaaggca ccctgcccat aaaaccagga tgtgcatacg tacacacaat cggtgtctgg
    13381 ttatggtttt ctaaacacta cataaggatc tgaatttttt agtgtgccaa aagcaaacga
    13441 taggtttaaa tgaaattgct cactaccaaa tcaagactct tcatatatat acgtgtgtgt
    13501 atatatatat gtcataacac tttcacgtgt gacagcctaa ggtttcttta gttttaggat
    13561 ggggtggggt gggagtggtt ttgcatgttt gaccaggatg tgatgcctgt ctttattcct
    13621 tgataagaag tagctgttac agcatttcaa gcaaattatt ttaggtagaa tagaacttac
    13681 tgccaaatga ttatttattt agcaaaaatt attctccaac ttttcgaatt cacaattttt
    13741 ctaagtgcat agagtagttt tttacattgt cacgaattcc ttaatacctt tatttaaaat
    13801 ggaaaaaata tggacaatat tttagaaata tgtgctacac atctaatttt atctgtagtt
    13861 taagtgatct aaattgagat gcctttgata tgaagagatt taccaacatt atatgcactc
    13921 gtgccttcaa tgtggaatca aactggttaa cctcagcaca gtcctctcgt ttctgtgttt
    13981 ctgctgcact aattgccagg gggtggggga gaaggcgaat gaattaattc atagtagaag
    14041 gaggcgatag gtgcagcaaa gggcagccac agtgttgatt ccacattata atgttgttgc
    14101 ctcttcttgg caaaagacac cacattgtgg gaagatctca gcttccaggg taaaagttaa
    14161 tttataactt aaaagtgcta ttaagttttt attaccaaat atatctttta tggtttatat
    14221 tgtagtggta tgtatgaaac atttaaaatt ttactgtgga aattgtgtat atatatatat
    14281 atagtcgaaa taggtgttca caggtcacat gtgaacggag aactgcatga ccgtacatga
    14341 aatgcaataa accaactgga aaaagtgcat gtgcttcatc ctctcaagcc aactgcagct
    14401 ggaaagtgct gcttatcctc cacccccaga aaatgcatgt atcaatatga gaataaagaa
    14461 cgcacacttt caattttatt gaggctttca acactattta aaagaaaatg taagaatttg
    14521 acattctgga gttattataa cattagaaaa tgagcataac attcactctg attttagcca
    14581 ttaagggaga ttagtaaaca gactgctaca gtgttccata gttggactgt gcatccaaaa
    14641 cattttttta tctttaataa atggtacagt ttttatgtag ttttcgaatg taagaagaaa
    14701 ggaatgctga ccaaaacttg atttcatcag cttcatgaaa aggactagtg tcattaacct
    14761 gttgaacaga attggtttat taaaaaaatc atttccagta gtgtgaaacc tttacgagtc
    14821 tttaacatct aaatgttatg actccttgta ccttaagttt tccagtcttt cttatttata
    14881 tcatctccaa gtacctctgg ctcctttcct cttgctcacc ggaaccttag ttttcctcaa
    14941 cagaatgctt tgttaaagta gcccacagtt gcaggatcca tagcaccgtc gtgcagacta
    15001 gcagcccaaa ggtgtgtttg gtttggctta tacggtgttt tgctttttaa actacttgcc
    15061 ataatttaaa agtggcaaca ctagacttaa aaaaaaaaaa gtctgattgc ccatattaga
    15121 tttttttttt aattcttcac aaaatctgct cttcctgaaa gatcaaagtg tctagaaagc
    15181 ccaaacatgt attcttaaca tagtaggcac cagctgaaac tgagtaatta aacggtcccc
    15241 tgaagccaag tattccctgg ttagtcgcca ccccaccact ccttcctgtc tctagtgtca
    15301 cacttgggct gttgattttc ttactcttct cttgctttaa tccttccccc cggctctctg
    15361 gctcctgtgg atatctgtgc ttgtttcctg gtccaggatg gtgatctgac tttcaaacca
    15421 gcttctcaaa aggggtgaca taaaatcagt tttgatgttt ttcctcctga aaaatcagat
    15481 gaatatttta gtcactgtta ctcatgcaca ttgtgttctt atgtttacag aagtgcttaa
    15541 gtgaatggaa gcactagaca tttggatttc cttcctaacg taatttttaa tgattacccc
    15601 ttttatacag taatttgtgg tctttagaaa gcagttaaac taattgacca tctaatagtt
    15661 gtactataca tatgtctaaa ataatagtca tggtaagttt ggcatcatat cttccccaaa
    15721 aaatgtttat taaaattaga ttattccagt ttaatgctat tttgtgaact gtataccctc
    15781 tgaaagcgct tatttttaca tgctacacaa cagttccaat tttaaggagt gtctcctaaa
    15841 attgggatga aaatctactg tagtctgttt taaagtatgc tatactatgt tcattggtta
    15901 cttaactgga tattaatata aaggttatta caagaaaaat gatgaagagc aaaaggagaa
    15961 gaaaatattt gcaagtgaat ccacaattct tgcagaacta tttgagttga tactaaagat
    16021 tttatgttca ctcctttacc ttagaactgt caagcttggg aatgaggaaa gtgccttggc
    16081 tgtgcttgaa acctgaattt tagtgcttcc cttattacat tcattgtttt caatgattga
    16141 tttataaaat taagacatac tggtagtaca agttgaaagt tggtttgaat acattttaat
    16201 taaatgataa tatgttaata tgcttttgtt cattgctttc tcactgaggt aaaacagcat
    16261 taaaaagttg tccagaattt aaa
//
