LOCUS       NM_206854              15336 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens QKI, KH domain containing RNA binding (QKI),
            transcript variant 3, mRNA.
ACCESSION   NM_206854
VERSION     NM_206854.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15336)
  AUTHORS   Zakutansky,P.M., Ku,L., Zhang,G., Shi,L., Li,Y., Yao,B.,
            Bassell,G.J., Read,R.D. and Feng,Y.
  TITLE     Isoform balance of the long noncoding RNA NEAT1 is regulated by the
            RNA-binding protein QKI, governs the glioma transcriptome, and
            impacts cell migration
  JOURNAL   J Biol Chem 300 (8), 107595 (2024)
   PUBMED   39032650
  REMARK    GeneRIF: Isoform balance of the long noncoding RNA NEAT1 is
            regulated by the RNA-binding protein QKI, governs the glioma
            transcriptome, and impacts cell migration.
REFERENCE   3  (bases 1 to 15336)
  AUTHORS   Wu,W., Cai,Y., Jin,J., Chen,Z. and Teng,M.
  TITLE     Circular RNA Circ_0001322 inhibits gastric cancer progression by
            modulating the miR-1264/QKI axis and suppressing the Hedgehog
            pathway
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (5), 190-197 (2024)
   PUBMED   38814217
  REMARK    GeneRIF: Circular RNA Circ_0001322 inhibits gastric cancer
            progression by modulating the miR-1264/QKI axis and suppressing the
            Hedgehog pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 15336)
  AUTHORS   Wang,H.F., Zhou,X.F., Zhang,Q.M., Wu,J.Q., Hou,J.H., Xu,X.L.,
            Li,X.M. and Liu,Y.L.
  TITLE     Involvement of circRNA Regulators MBNL1 and QKI in the Progression
            of Esophageal Squamous Cell Carcinoma
  JOURNAL   Cancer Control 31, 10732748241257142 (2024)
   PUBMED   38769028
  REMARK    GeneRIF: Involvement of circRNA Regulators MBNL1 and QKI in the
            Progression of Esophageal Squamous Cell Carcinoma.
REFERENCE   5  (bases 1 to 15336)
  AUTHORS   Min,K.W., Jo,M.H., Song,M., Lee,J.W., Shim,M.J., Kim,K., Park,H.B.,
            Ha,S., Mun,H., Polash,A., Hafner,M., Cho,J.H., Kim,D., Jeong,J.H.,
            Ko,S., Hohng,S., Kang,S.U. and Yoon,J.H.
  TITLE     Mature microRNA-binding protein QKI promotes microRNA-mediated gene
            silencing
  JOURNAL   RNA Biol 21 (1), 1-15 (2024)
   PUBMED   38372062
  REMARK    GeneRIF: Mature microRNA-binding protein QKI promotes
            microRNA-mediated gene silencing.
REFERENCE   6  (bases 1 to 15336)
  AUTHORS   Wu,J.I., Reed,R.B., Grabowski,P.J. and Artzt,K.
  TITLE     Function of quaking in myelination: regulation of alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 99 (7), 4233-4238 (2002)
   PUBMED   11917126
REFERENCE   7  (bases 1 to 15336)
  AUTHORS   Li,Z.Z., Kondo,T., Murata,T., Ebersole,T.A., Nishi,T., Tada,K.,
            Ushio,Y., Yamamura,K. and Abe,K.
  TITLE     Expression of Hqk encoding a KH RNA binding protein is altered in
            human glioma
  JOURNAL   Jpn J Cancer Res 93 (2), 167-177 (2002)
   PUBMED   11856480
REFERENCE   8  (bases 1 to 15336)
  AUTHORS   Saccomanno,L., Loushin,C., Jan,E., Punkay,E., Artzt,K. and
            Goodwin,E.B.
  TITLE     The STAR protein QKI-6 is a translational repressor
  JOURNAL   Proc Natl Acad Sci U S A 96 (22), 12605-12610 (1999)
   PUBMED   10535969
  REMARK    GeneRIF: There are many isoforms of this RNA binding protein. QKI-6
            has been shown to be a translational repressor.
REFERENCE   9  (bases 1 to 15336)
  AUTHORS   Chen,T. and Richard,S.
  TITLE     Structure-function analysis of Qk1: a lethal point mutation in
            mouse quaking prevents homodimerization
  JOURNAL   Mol Cell Biol 18 (8), 4863-4871 (1998)
   PUBMED   9671495
REFERENCE   10 (bases 1 to 15336)
  AUTHORS   Ebersole,T.A., Chen,Q., Justice,M.J. and Artzt,K.
  TITLE     The quaking gene product necessary in embryogenesis and myelination
            combines features of RNA binding and signal transduction proteins
  JOURNAL   Nat Genet 12 (3), 260-265 (1996)
   PUBMED   8589716
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB067801.1, AF272349.1,
            AB067800.1, AL031781.1 and AW131362.1.
            
            On May 31, 2019 this sequence version replaced NM_206854.2.
            
            Summary: The protein encoded by this gene is an RNA-binding protein
            that regulates pre-mRNA splicing, export of mRNAs from the nucleus,
            protein translation, and mRNA stability. The encoded protein is
            involved in myelinization and oligodendrocyte differentiation and
            may play a role in schizophrenia. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (3) differs in the 3' UTR and
            coding region compared to variant 1, which results in a frameshift.
            The resulting isoform (3; also known as HQK-7 and QKI-7) is shorter
            and has a distinct C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB067800.1, SRR1803612.57377.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               AB067801.1         76-479
            405-1410            AF272349.1         362-1367
            1411-6889           AB067800.1         1487-6965
            6890-15293          AL031781.1         4352-12755          c
            15294-15336         AW131362.1         21-63               c
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q26"
     gene            1..15336
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="QKI, KH domain containing RNA binding"
                     /db_xref="GeneID:9444"
                     /db_xref="HGNC:HGNC:21100"
                     /db_xref="MIM:609590"
     exon            1..618
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             477..1454
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="isoform 3 is encoded by transcript variant 3;
                     homolog of mouse quaking QKI (KH domain RNA binding
                     protein); RNA binding protein HQK; quaking homolog, KH
                     domain RNA binding; QKI/LOC100132735 fusion; KH
                     domain-containing RNA-binding protein QKI"
                     /codon_start=1
                     /product="KH domain-containing RNA-binding protein QKI
                     isoform 3"
                     /protein_id="NP_996736.1"
                     /db_xref="CCDS:CCDS5287.1"
                     /db_xref="GeneID:9444"
                     /db_xref="HGNC:HGNC:21100"
                     /db_xref="MIM:609590"
                     /translation="MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERL
                     LDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRI
                     LGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDA
                     QNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSL
                     AATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVP
                     PGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLEWIEMPVMPDISAH"
     exon            619..761
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            762..878
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1022
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1110
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1410
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..15336
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6864..6869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      6889
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="major polyA site"
     regulatory      10517..10522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      10540
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="major polyA site"
     regulatory      10596..10601
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      10608
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      13399..13404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      13420
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      15252..15257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: ATTAAA"
     polyA_site      15271
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      15312..15317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: ATTAAA"
     polyA_site      15336
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
ORIGIN      
        1 gcagtcggag cgcggcggca gcggcagagg cgccgcggcg gggaccagcc cagagagacc
       61 ccccgagccc gcggcacagg cggcggcggc gctgagcggg ctcgaccagc cgagcgagcg
      121 gcggccccgg ctcctcctcg tccggcgcca gcagcccgcg ccccgcgccc gcactcggcc
      181 agccgagacc cgcctcccgc ccgcccgccg gcctcgtgag ggacgcgccg agccgggcgg
      241 ccgagagcgg cggcggctgg gagcgcgtcg ggcccgggcg gaaagtgcct gcggggggcg
      301 ggcgagcgcg cggtgccggc cgccccgggg ctcggcgcgg gagccagagc gggagccggc
      361 gcggagcggg acgccgggtc ccgagcggcc cgcggccggg gctcgccccc gcccctccct
      421 cctctccggc ggcggcggcg gcggcggcgg gcggagtgag ctgcggagcc tggaatatgg
      481 tcggggaaat ggaaacgaag gagaagccga agcccacccc agattacctg atgcagctga
      541 tgaacgacaa gaagctcatg agcagcctgc ccaacttctg cgggatcttc aaccacctcg
      601 agcggctgct ggacgaagaa attagcagag tacggaaaga catgtacaat gacacattaa
      661 atggcagtac agagaaaagg agtgcagaat tgcctgatgc tgtgggacct attgttcagt
      721 tacaagagaa actttatgtg cctgtaaaag aatacccaga ttttaatttt gttgggagaa
      781 tccttggacc tagaggactt acagccaaac aacttgaagc agaaaccgga tgtaaaatca
      841 tggtccgagg caaaggctca atgagggata aaaaaaagga ggagcaaaat agaggcaagc
      901 ccaattggga gcatctaaat gaagatttac atgtactaat cactgtggaa gatgctcaga
      961 acagagcaga aatcaaattg aagagagcag ttgaagaagt gaagaaatta ttggtacctg
     1021 cagcagaagg agaagacagc ctgaagaaga tgcagctgat ggagcttgcg attctgaatg
     1081 gcacctacag agatgccaac attaaatcac cagcccttgc cttttctctt gcagcaacag
     1141 cccaggctgc tccaaggatc attactgggc ctgcgccggt tctcccacca gctgccctgc
     1201 gtactcctac gccagctggc cctaccataa tgcctttgat cagacaaata cagaccgctg
     1261 tcatgccaaa cggaactcct cacccaactg ctgcaatagt tcctccaggg cccgaagctg
     1321 gtttaatcta tacaccctat gagtacccct acacattggc accagctaca tcaatccttg
     1381 agtatcctat tgaacctagt ggtgtattag agtggattga aatgccagtc atgcctgata
     1441 tttcagccca ttgacttgct ggatgaagga ctagaataca gcagctgtta taacacgacc
     1501 agtcaatgtg gaacaaactg tttctgtgca acccctttgt tttaccagac aaaatttgaa
     1561 tacttttttt cctgaattgt atatgacctt ggtgctgcat gcatgctgtt gacttttagg
     1621 actttgatct tttaaggttt tttttcccca gcattaatat tgatttataa agatttgaaa
     1681 atctttaatg aactggagaa cactaagatt taaactcgaa aattcgttgt tcaagtaaag
     1741 aaagccatga tgctctgtat gttatctgtg tgtgtgcatg cactcaggtg ccctttgttt
     1801 catgaacaaa tacatttcat tgtacatgtt ttctgtttaa atcattgtat aaagtaattg
     1861 caggtcagaa ttataccaca gaactgttta tgagaggctt gtgtctgttg cacatttctt
     1921 gaagcatttt taaaataaca tgtaacctgt aaccttgttg tttaagtttt cttttctatt
     1981 aatactctgt cctgtggtcc cgtgcatgct ccttttccca gaactcctct ctgctgcacc
     2041 cacagcatct gttcccgagg agttatgact cttgacttcc tgcagggctg gggctcttag
     2101 ccaccagctg ctgttccagc actttcagcg caagatctcc ctgattttgc cacgtggaat
     2161 tgtacttgta tatgattacc ttatctaaaa tgaataagag gtgatggacc agtttactgc
     2221 ttagaaatag caagaggcac tgcagtaaaa cttgtttctc attgtaaagc ttcatgtctt
     2281 ttgtttgttg gaaaattttt acttatagaa acttaattat tagactggta aaataaagac
     2341 caaaatatgc agatttctaa ttggcattca taaggtgaat aataataagt gcccaatgaa
     2401 aaaatctatt atggttaatt tcatttcttg ctttgccacc taagcagtaa aacatgatat
     2461 tgaccacttg gagaactcag aaaattattt taaatttcta agttataata aatttgcaca
     2521 cagataacat gcatgctatt tatgtcacat ctcacattaa attattttaa aataagcagt
     2581 gcccttcaaa acagatgcag acatgtgtgt tggtagtagt gaggagattg gtattagcat
     2641 caagtcttca ttgatgacta atttttaatt cccttccttt tatctttagg tatggctttc
     2701 ccaacgaaag gctaagaatt caagaacggt cttaactgaa ccctcatcag atctgaattt
     2761 aacaaatgct tagtctcagc agcctccggg ggaaaaaagc ttagcctagc agtcagtgac
     2821 ttacttgcac tttttgcaca tagatataaa gtaaaattat gttattaatt tggtttagtc
     2881 tgtaatatta cacagtaatg gtaatttata aaggagtgta tagtagtata ctgactgcta
     2941 agtgtactat actgtttgct ttactaatca cctaattcat tgcagttcat acttattgac
     3001 tcagagttta aaaccacaat ctgtaggctt taagaattgc ttttaaacct ttttaagtga
     3061 taaactcttg aagtggtctt aaggttagca aataattgtc tgtattaaac atggcgttat
     3121 ttaagtagtc ctgctgcaag aaaggcactt cagtgaatat gtgactagta aagcttaaat
     3181 atgtttggat ctaatagagc tcatgaaata ctgtaacttg gggattgtaa atgaatggat
     3241 acaataggtt aaggcccaag atgtttgaaa tgaatgcaat attaatagat gcatatatac
     3301 atgacatatt gtggttaatt ttaaaactac tgtgccttaa cgtgtttctt aaacttttgt
     3361 agtaaatgaa catttgaaat ccattttgat aaacctgctg ttaatgtttt ttcccccctt
     3421 gtgaatgttt tctaactttg tcttggtaat tgcaatttaa ctaggtgcgg tggctactaa
     3481 agttcgaagg cacgatatgc gtgtccatcc ttaccaaagg attgtgaccg cagaccgagg
     3541 ttagtttagt tctgcagttc ttgtctataa gaaatgcgtt gggtgtccat aaaatttgca
     3601 acaccacacc tgatggaaaa atgtaattgc ttacctgcac acagtttttt ttccttttct
     3661 aaatttgttt gtgatcattg acagatttaa gggttgggtt ttttgttttg gtttggtttt
     3721 ttctcccttc atattttaac ttattaaatc ttctattatt atgtactatg atttattcct
     3781 actaaaacat aaattagtca atttggggag ctattaaatt ttgatgttgc ctcagttatt
     3841 ttcattaata acttttgcta aaatttacct tttaaaagtt ctgaaatttg gggttggtat
     3901 tgcaaaatga attgaacgga tggtgcttta atgagagaag taaaggaata tatgcctcct
     3961 gtgtactttt ggagaaaact gaagatttag tttccatttt tctttataat aaaatatttg
     4021 agccactgta taaacatgag ggtgcacatg tcaagtactt agctaaaatc aacaaagtga
     4081 ggactgtgaa gataactttc atgtagttgg atttttttat ataaattaga gcgtcaattc
     4141 ccttttattc ttacattctc ttgccaaaat ggatcctttg atttttgtca tttgctgcta
     4201 aaatatatta tctgaaggta aactgtctct tgaagttctg tggtggtatg agatccagct
     4261 gtgtgaatat aatttttgac taacctgtga ctggaaaata agtcttcatt tttctccttt
     4321 acaactttta taatttgtgt cctgtatatg cccttggacc cttttataaa ttatttttgc
     4381 ttattgagga atggtatttt cggtttattg acattgaatt gttttctaag tttttccatc
     4441 acaagatagg aatatcaagc atggtagtaa aaaaggattt tttacaagtt tctattatgg
     4501 cacatttgtt tcctacaaat taaattgatc aaagtctcaa atattagtgg aggaaaaagt
     4561 gctggatttg tacttcaatt gtacatcttc tatgaattta tgaaggacct acctgcttta
     4621 tgtgattacc ttgagtactg atgataaata ctagacacag ctgttgtcca catcagaaat
     4681 aacatgcctt tcccaaagac attgttaata tttgtttagc ataatccata aagaacgttg
     4741 aagactttta aactaatgta ttcatcacta acagtgattg ttcagtgaac aaaatagacc
     4801 ccagctccgt ggtcgtctat gaggatggta ttccttgtaa gtgttctgta tgagcacgtc
     4861 tcatagggat ggggaagttt tttggaaagt cacttgagtt ttaaagaaac tggacaattc
     4921 catgaataat ttgaggtttt attcatattg ctaatgtcta caattgttct aaagagtatt
     4981 ttaaagtagt ttttttcccc cagctaatct ttccctatgt atgccccctt ttaggagatt
     5041 tctcaccatt ttgctttaac acaataatta ttggcctact taatactcca gtgccttgag
     5101 atagttggac atattttagg aagattttat tgtatttgga aaattacgac acattccatg
     5161 catggatctt ttgtagttac gtgttttgat tagtcctgtc actgttttta tttaaaatgc
     5221 agtgtcagca aaccgaaatg ctcatttctt ttttagtaac agagtactcg tatacttatc
     5281 actgtatgca ctcattgggt ctaagggaga tgtctgaagt ccttcactta aattatttta
     5341 tgatactact gttttctcta tttttgaggt taaaattatt ttaaaaaaat attttgaaga
     5401 gaagtttatt agtataagca ttcatttaat gcaaaaaaac tcagcccttg gacatataac
     5461 catgtaattc tatatgaacg tttagagtac tacatggttg accaatatct agttctttac
     5521 taaaagtaaa ctcacctctt tatatatatt tcaaaactga atttgaagcc tattagagac
     5581 caactgacct tttagttgta atgaactgta tggtcttgca tggtgacaca cctgtgtagg
     5641 aggccaagac ttggcagagg atgtagtcta ggtgtcctgg tatatataaa tggtcactca
     5701 cttcgtttat atattccaca ctcagaaaag aagtcagagt tttttctgag taatacaaaa
     5761 accatcactt acataatttg tataagaaac tttaaacagt tttaatcaaa actatgagta
     5821 acatcaaaaa tgctttaaga ttctatattt tggaatacct gtgaagtagt atgactacat
     5881 catgttaaat atttattttc ttctatgatt ttggagcagc aatttttatt ttaaataaaa
     5941 ttccactttt aagaaattca gggaagattt ggtcacattg aagatacagt atttttgtag
     6001 tatttataaa ctgttctaaa tgatagacta tagaaaacat ttttgtcata tgaaggtaaa
     6061 tcagtccatt attttggatc atttaaactg aacattacac cttctgggct ttgatttatg
     6121 aagtggcaca gaatctaact ttgcactgaa tatcttttca ttcacatctc ctctgcctta
     6181 gtcacaatgg caacagtaca gaaaattcta tcaaacctca gaatatagta gaaataatta
     6241 agctgttgaa tgagtcttaa aaattatact actgttaagt ggaccaagtt tggtgaagca
     6301 gaatgtgaca aaggttgatt aaggaaggaa caactcaagg acattgggaa tgataacttt
     6361 tccacttgag aactacttta tgttttactg taatttttaa agtttttttg tcctttttgt
     6421 tattttgcaa aagaaaatag tatttacagg tggcttcttt taaaatataa aaatataaag
     6481 caggaatgta tatgaaatgt cagattttat tgtatttgca gagtattagc tttgaaattg
     6541 aataaagaaa gctgtttgta gttttaaaat gccttattgg tatcaattag aaatttcttc
     6601 tattttttga tgtacttaga gctttttgag tgtagaattt taaatggcag gattttacag
     6661 tgtttacatg caagtgcatt ttataagtgt tctatatgtg taaaatagta ttttcaactg
     6721 gaaagtgttg gctagtgcaa aaggcctggc cattttctgg ttcccatgat gttgcctaca
     6781 ctgctagact acagtttagt atcgctttgt atcatgaggc caagaaattc catgttgttt
     6841 gtaaatagaa taattgaaaa agcaataaac atttattgaa caaaagaaac cttgtttggc
     6901 ccagagttta tgttgaacca aatggttcat agaattttga atttctttag attcatttct
     6961 gttaatcatc agttattaat aatcacatcc atcattaata gttgctttaa tgcttgcatc
     7021 ttagagaata ctaatgctta atagcagtca gatactgata actgcactga ctgtttaaaa
     7081 attctgtata ccaaacctat tcattgttgt ttttcaattt ccttcattga ctgctactaa
     7141 ctattagttg ttagttgtat tttatttcta ttttttcttg ttaaccagtt tttttttttt
     7201 ttttgtggga gtgggctctt cttcatttgt tcaaatcatg atacgaaatt gtcatgattt
     7261 taattgtatt ctgtagatgg tgtcaatcag tttgtcagaa ttaaacatgc tttttttttt
     7321 attagttgtg attagttttt catgttgtca ttaagccgtc actagctgaa actaatctct
     7381 tctctatctt ttcttttctt ttttttgttt tgtttttttt tgtttctaac cacccagccg
     7441 ccaccggcaa ctaacctatg accttctgac ctctgaactc ttcacccaat gatgacctga
     7501 ccatgcctgc ctgctgatca gttaactggt aatcgccttt gcttgcctgt cgtcagtgca
     7561 gcgagctgag gcacttgtcc gttcgtctta ccatctaacc aaacaaaaga caaagaaatt
     7621 gttgtcctcc aactcagctt tttttttttt tttttcctgt ttgggtgaaa gtggttctag
     7681 aaactgcact gaatagtagt aaagcaataa ggcccaattc atcccacagc actgatcatc
     7741 ttttaatatc ccaccctaag cgaacggtaa gaaggcctct cttaagaagg ggagacagat
     7801 ggtccttaac tactcaatga cagaggcagt tactgtgaga gacttctagg aatctttttc
     7861 ttctcatagc gaagtcaaag ctctctctga atgtactgtg tgatgatgca tcatgcatga
     7921 accttcggtc agggatatca ttggtgaagt gatttcaaaa agtattcaaa atttgatatg
     7981 ctgtttagtc actacagtgc cctcaaaggg cagaagttgc agcctttttt atattgcctg
     8041 ccaaaatttg aagtattaga agaaagtgtg ccatgagaga aaaacttaag gagttttgaa
     8101 aagtaatgca aataacaaaa ctgcaacact atttttaaaa agataaatat ctgagttaaa
     8161 attactgaat ctttatttta cacctaaaaa aatatgagaa caaggtacat gcattatgtg
     8221 tcacattact gggcaaactg ttcaagtatt tttttttaaa cctccctgta tagaaaaaaa
     8281 tcattaagga tgtaaaagcc atgcttgcct atttgctgta tacatgtaat gaaattgtag
     8341 ataaagtgta gtgcattgaa acaaatgaac aaaaagtaga tacttttact atacaagggt
     8401 gctggtgcag aaaaaaatat atatattttt ggaaatgtag cattttatac tttcaagtgt
     8461 tataaaaaaa aagaaaaaga acaaagaaac cctttatttc attaaatgat tttttctggt
     8521 ggttgtcaag aaacaaaaga ccaaaagagc ctttttgtct ttctttttta ttttttaagt
     8581 attggaataa gtctaaagaa aagaaagtgc cttattttcc ttcatggtca ttcagtcaag
     8641 tgtctcataa agatcagctc ctccctcaga atacaagtta atgcatgtta ctcagtcgcc
     8701 cagtatgtga aagaagatat gagggagaca aatgagttga tagtttgaat tataggattt
     8761 ttgccacgtt accttgatgc aaatttgcca aatctgatac tgtgaaaaga tttgataact
     8821 tttctaatgc ctgactagta agttttaaaa catcattctt taaaagaata gtttaatact
     8881 tttggttatt ccaatcttca atgttaaccc aaaggattct gagataaaaa ttatgtgtca
     8941 tctaaacttc agtttacaga caaaattaga gctttgtttt atttggagaa gggggatgca
     9001 ttcatttgat tttgtttttt gtttttaatt aattgaatat aataggatac ctagcaaatg
     9061 ttttcacaaa agtgaatctt tgtgattttc attctagaaa acaatgtttt aaatcaccca
     9121 aatttaatca catgtcttta tatcatacca gataactggt gtagtgtatc cttttactgt
     9181 ttcaataacc tctgcctccc ccacccctta actttaaaag aatgtgaaaa actagcatca
     9241 tagtgcatat aaatactcaa ccacatttca gcttaagctt ctaatttctc aagaagttga
     9301 aacagttagt tatgtagttg aagcaatttg tgtgcaaagc ctacatctaa acacttgagt
     9361 gaataatcat taactgctca gtaccagacg tggcaaatct caagtgacag tggacccccc
     9421 cccccgccca gcttatcaac tcgtcccctc ttccacacct tcccagcatc acagtcagag
     9481 gtcagcagga agctgagtca ttaccttctt ggtgacctcc cccaccatct tgtctcactt
     9541 ggaaaccgtt gtcagtttat ttactatgca atagacattc attgttttgt atccagctag
     9601 ctttggttta atatgccact gctttgtctt tctgttacac atacagtttt gatttattta
     9661 caatatatgc tgtgccattt tgatttttat attgtttggt tggttgaata aatttgcgac
     9721 actcaaacac ttgaggtgat agttgttaaa aacaatacca gtatttgatc cagtttcatc
     9781 tcaactatgt tatacctgga cattttagat gtttatcaaa ggtcttttat ggggaagaaa
     9841 gtaaatgtat gaaataataa atgtgtgaca tttataggga atgttggctc tgaacaaact
     9901 tttgaaaact tggttgacaa aattttggat caactgaaaa aagcatgact tttgaaatct
     9961 ctgaatgcct tggttctcag tattatcatt ctttattgaa tttatttctt attaaaatat
    10021 gtagttttta agactttttt tctgacagta ttatgtaatt ttttagcgtg ggtagatggg
    10081 agtgtcgctt gtatgttatc gtacagctga catgtatttt tgtctattct ttattatctt
    10141 agtttcatgc tatgtatgta ccataaccaa cctattgcct atgagaaaca tgtaagataa
    10201 tgtatttaca gccattgtta caagtttata atgtattttt ctatcttgtt ttatatgtat
    10261 gttatataac attcaaaaag aatttttttc ttgattgaga aaaggataca aaatgcaaaa
    10321 tccacaattt tgataactga aaattgccaa ttgttttgca gtactttatt atattgggtg
    10381 tcttgtcttt tttgggcttt tagttagcta atgaatgaat acattagaca cttttgggtt
    10441 ttagttggga ttttacatag cttgcatttt aattctttgg ttctttgctg tttctattaa
    10501 cccacagcat tattttaata aatgttataa taccaaccta ctaactctgg actttgtctg
    10561 tttattaacc tttatatgtt taattaaaat aaacaaataa agacaaaaga atgtaaagtc
    10621 ttgagttact ggactaaccc tgaagaacgt gaccacagat aacatagtgt gacttgtttt
    10681 tatgttgttt gtcagctgac agttttgcac actactgtta aaatggcttt ggttattctg
    10741 gtcttttagc ttgggggccg gggcgggtgt gggtgtaggt gcagtagaga tgggaacaaa
    10801 gtagcctttg tttgaggcat tcttctcttg ccacttgcct ttcagcatct gcattactaa
    10861 ttccacacct tatttttcct tctccaaaat aatcactgtc tgctcacagc atcgagtaaa
    10921 aagatacatt gtcatggtat atacctggag aaaccagttt gattctttgt tgaaccattt
    10981 cttttaattt ctgtaaataa gtaattttat ggtcacattg taaagcagag aaactgttga
    11041 tattttacct ttatttagat ttttgtttta acttgacctt tttcctttgg aaagcgttga
    11101 acttgtttag ctaactactc ctattagggg agcttaactt gggtaaagtc aaaggcccac
    11161 tggctgtttc tcatggtggc ctgaagcccc gctgctttcc agtgaggaaa agaaccctca
    11221 gcacgttact gagctttcct caggtatttt tttctctgtc acctacttta caaatacagt
    11281 cagactaaaa cattaaacaa gaagtttaag ggggaataac attgggattt gaaaagttgt
    11341 atgtgattaa tttcttgcat gcaaatcaat ttaagatttc ttaaatatct tcatgctttt
    11401 cttggctgga acttgggtaa attaacctgt aattatgaat tatttagttt aactcaaggt
    11461 gcatttttat ttcacagatt gatacttgct tttttctgtg aaacagttct cctagatacc
    11521 aaaatcctca ggatgaattt ttgcatttgt aaataacaat ttatctttgt aaattacatt
    11581 ttatttattt agagttctaa aattgagggc tgaaatgaat gacatgtaat agaagacaaa
    11641 actgtaccaa tacgctgtta accaatcaga ctgtccaggt attctcattt cataaatatg
    11701 tatacacaca tataaataca cagagtagtg ttagctgatg tgagtgataa cagcgaatca
    11761 ccattgagat ggtcatttgg atcatgtaaa caaaccgtgt catgtctctg atgggagcgg
    11821 tgggtagtgt actgttaggt ggacaggtct tttaacatgc tactatttgt aaaagtttcc
    11881 gttgccataa cagttcatgt agtagtctgt gaaaattcga agctgtctgt caagctgcta
    11941 atatacaagt tgtcatggta ccatgtaact taaattgatg aatttctatg caatttacta
    12001 ttctctgaag cctatgaatg cagcctcatc tcattttcag ttaagggtac tttatgtata
    12061 attatgtcat tgctgccttt tatgtaggaa atgtaaaaaa gttttaaaag gcgaaaataa
    12121 cattacagat ttcattttta tacaggttac aatattaaac atgaaactac caaattccaa
    12181 gaatagtaaa attctagagt cagttgattg attgaaacta tttgacaaaa cagttgaaag
    12241 ctgagctttc agtcaagaag ttggtgaaat taatgtcctt aaacttttcc tttaggcaaa
    12301 atgtcaggga aaacttaaca tgaaagtagt tactcccata cgcaccagtg cagtagctcc
    12361 aggtgtaaga ggtcacgaag gcaccctgcc cataaaacca ggatgtgcat acgtacacac
    12421 aatcggtgtc tggttatggt tttctaaaca ctacataagg atctgaattt tttagtgtgc
    12481 caaaagcaaa cgataggttt aaatgaaatt gctcactacc aaatcaagac tcttcatata
    12541 tatacgtgtg tgtatatata tatgtcataa cactttcacg tgtgacagcc taaggtttct
    12601 ttagttttag gatggggtgg ggtgggagtg gttttgcatg tttgaccagg atgtgatgcc
    12661 tgtctttatt ccttgataag aagtagctgt tacagcattt caagcaaatt attttaggta
    12721 gaatagaact tactgccaaa tgattattta tttagcaaaa attattctcc aacttttcga
    12781 attcacaatt tttctaagtg catagagtag ttttttacat tgtcacgaat tccttaatac
    12841 ctttatttaa aatggaaaaa atatggacaa tattttagaa atatgtgcta cacatctaat
    12901 tttatctgta gtttaagtga tctaaattga gatgcctttg atatgaagag atttaccaac
    12961 attatatgca ctcgtgcctt caatgtggaa tcaaactggt taacctcagc acagtcctct
    13021 cgtttctgtg tttctgctgc actaattgcc agggggtggg ggagaaggcg aatgaattaa
    13081 ttcatagtag aaggaggcga taggtgcagc aaagggcagc cacagtgttg attccacatt
    13141 ataatgttgt tgcctcttct tggcaaaaga caccacattg tgggaagatc tcagcttcca
    13201 gggtaaaagt taatttataa cttaaaagtg ctattaagtt tttattacca aatatatctt
    13261 ttatggttta tattgtagtg gtatgtatga aacatttaaa attttactgt ggaaattgtg
    13321 tatatatata tatatagtcg aaataggtgt tcacaggtca catgtgaacg gagaactgca
    13381 tgaccgtaca tgaaatgcaa taaaccaact ggaaaaagtg catgtgcttc atcctctcaa
    13441 gccaactgca gctggaaagt gctgcttatc ctccaccccc agaaaatgca tgtatcaata
    13501 tgagaataaa gaacgcacac tttcaatttt attgaggctt tcaacactat ttaaaagaaa
    13561 atgtaagaat ttgacattct ggagttatta taacattaga aaatgagcat aacattcact
    13621 ctgattttag ccattaaggg agattagtaa acagactgct acagtgttcc atagttggac
    13681 tgtgcatcca aaacattttt ttatctttaa taaatggtac agtttttatg tagttttcga
    13741 atgtaagaag aaaggaatgc tgaccaaaac ttgatttcat cagcttcatg aaaaggacta
    13801 gtgtcattaa cctgttgaac agaattggtt tattaaaaaa atcatttcca gtagtgtgaa
    13861 acctttacga gtctttaaca tctaaatgtt atgactcctt gtaccttaag ttttccagtc
    13921 tttcttattt atatcatctc caagtacctc tggctccttt cctcttgctc accggaacct
    13981 tagttttcct caacagaatg ctttgttaaa gtagcccaca gttgcaggat ccatagcacc
    14041 gtcgtgcaga ctagcagccc aaaggtgtgt ttggtttggc ttatacggtg ttttgctttt
    14101 taaactactt gccataattt aaaagtggca acactagact taaaaaaaaa aaagtctgat
    14161 tgcccatatt agattttttt tttaattctt cacaaaatct gctcttcctg aaagatcaaa
    14221 gtgtctagaa agcccaaaca tgtattctta acatagtagg caccagctga aactgagtaa
    14281 ttaaacggtc ccctgaagcc aagtattccc tggttagtcg ccaccccacc actccttcct
    14341 gtctctagtg tcacacttgg gctgttgatt ttcttactct tctcttgctt taatccttcc
    14401 ccccggctct ctggctcctg tggatatctg tgcttgtttc ctggtccagg atggtgatct
    14461 gactttcaaa ccagcttctc aaaaggggtg acataaaatc agttttgatg tttttcctcc
    14521 tgaaaaatca gatgaatatt ttagtcactg ttactcatgc acattgtgtt cttatgttta
    14581 cagaagtgct taagtgaatg gaagcactag acatttggat ttccttccta acgtaatttt
    14641 taatgattac cccttttata cagtaatttg tggtctttag aaagcagtta aactaattga
    14701 ccatctaata gttgtactat acatatgtct aaaataatag tcatggtaag tttggcatca
    14761 tatcttcccc aaaaaatgtt tattaaaatt agattattcc agtttaatgc tattttgtga
    14821 actgtatacc ctctgaaagc gcttattttt acatgctaca caacagttcc aattttaagg
    14881 agtgtctcct aaaattggga tgaaaatcta ctgtagtctg ttttaaagta tgctatacta
    14941 tgttcattgg ttacttaact ggatattaat ataaaggtta ttacaagaaa aatgatgaag
    15001 agcaaaagga gaagaaaata tttgcaagtg aatccacaat tcttgcagaa ctatttgagt
    15061 tgatactaaa gattttatgt tcactccttt accttagaac tgtcaagctt gggaatgagg
    15121 aaagtgcctt ggctgtgctt gaaacctgaa ttttagtgct tcccttatta cattcattgt
    15181 tttcaatgat tgatttataa aattaagaca tactggtagt acaagttgaa agttggtttg
    15241 aatacatttt aattaaatga taatatgtta atatgctttt gttcattgct ttctcactga
    15301 ggtaaaacag cattaaaaag ttgtccagaa tttaaa
//
