LOCUS       NM_006775               9384 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens QKI, KH domain containing RNA binding (QKI),
            transcript variant 1, mRNA.
ACCESSION   NM_006775 XM_945803
VERSION     NM_006775.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Zakutansky,P.M., Ku,L., Zhang,G., Shi,L., Li,Y., Yao,B.,
            Bassell,G.J., Read,R.D. and Feng,Y.
  TITLE     Isoform balance of the long noncoding RNA NEAT1 is regulated by the
            RNA-binding protein QKI, governs the glioma transcriptome, and
            impacts cell migration
  JOURNAL   J Biol Chem 300 (8), 107595 (2024)
   PUBMED   39032650
  REMARK    GeneRIF: Isoform balance of the long noncoding RNA NEAT1 is
            regulated by the RNA-binding protein QKI, governs the glioma
            transcriptome, and impacts cell migration.
REFERENCE   3  (bases 1 to 9384)
  AUTHORS   Wu,W., Cai,Y., Jin,J., Chen,Z. and Teng,M.
  TITLE     Circular RNA Circ_0001322 inhibits gastric cancer progression by
            modulating the miR-1264/QKI axis and suppressing the Hedgehog
            pathway
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (5), 190-197 (2024)
   PUBMED   38814217
  REMARK    GeneRIF: Circular RNA Circ_0001322 inhibits gastric cancer
            progression by modulating the miR-1264/QKI axis and suppressing the
            Hedgehog pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9384)
  AUTHORS   Wang,H.F., Zhou,X.F., Zhang,Q.M., Wu,J.Q., Hou,J.H., Xu,X.L.,
            Li,X.M. and Liu,Y.L.
  TITLE     Involvement of circRNA Regulators MBNL1 and QKI in the Progression
            of Esophageal Squamous Cell Carcinoma
  JOURNAL   Cancer Control 31, 10732748241257142 (2024)
   PUBMED   38769028
  REMARK    GeneRIF: Involvement of circRNA Regulators MBNL1 and QKI in the
            Progression of Esophageal Squamous Cell Carcinoma.
REFERENCE   5  (bases 1 to 9384)
  AUTHORS   Min,K.W., Jo,M.H., Song,M., Lee,J.W., Shim,M.J., Kim,K., Park,H.B.,
            Ha,S., Mun,H., Polash,A., Hafner,M., Cho,J.H., Kim,D., Jeong,J.H.,
            Ko,S., Hohng,S., Kang,S.U. and Yoon,J.H.
  TITLE     Mature microRNA-binding protein QKI promotes microRNA-mediated gene
            silencing
  JOURNAL   RNA Biol 21 (1), 1-15 (2024)
   PUBMED   38372062
  REMARK    GeneRIF: Mature microRNA-binding protein QKI promotes
            microRNA-mediated gene silencing.
REFERENCE   6  (bases 1 to 9384)
  AUTHORS   Wu,J.I., Reed,R.B., Grabowski,P.J. and Artzt,K.
  TITLE     Function of quaking in myelination: regulation of alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 99 (7), 4233-4238 (2002)
   PUBMED   11917126
REFERENCE   7  (bases 1 to 9384)
  AUTHORS   Li,Z.Z., Kondo,T., Murata,T., Ebersole,T.A., Nishi,T., Tada,K.,
            Ushio,Y., Yamamura,K. and Abe,K.
  TITLE     Expression of Hqk encoding a KH RNA binding protein is altered in
            human glioma
  JOURNAL   Jpn J Cancer Res 93 (2), 167-177 (2002)
   PUBMED   11856480
REFERENCE   8  (bases 1 to 9384)
  AUTHORS   Saccomanno,L., Loushin,C., Jan,E., Punkay,E., Artzt,K. and
            Goodwin,E.B.
  TITLE     The STAR protein QKI-6 is a translational repressor
  JOURNAL   Proc Natl Acad Sci U S A 96 (22), 12605-12610 (1999)
   PUBMED   10535969
  REMARK    GeneRIF: There are many isoforms of this RNA binding protein. QKI-6
            has been shown to be a translational repressor.
REFERENCE   9  (bases 1 to 9384)
  AUTHORS   Chen,T. and Richard,S.
  TITLE     Structure-function analysis of Qk1: a lethal point mutation in
            mouse quaking prevents homodimerization
  JOURNAL   Mol Cell Biol 18 (8), 4863-4871 (1998)
   PUBMED   9671495
REFERENCE   10 (bases 1 to 9384)
  AUTHORS   Ebersole,T.A., Chen,Q., Justice,M.J. and Artzt,K.
  TITLE     The quaking gene product necessary in embryogenesis and myelination
            combines features of RNA binding and signal transduction proteins
  JOURNAL   Nat Genet 12 (3), 260-265 (1996)
   PUBMED   8589716
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB067801.1, AF272349.1,
            AB067798.1, AL031781.1 and AW131362.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006775.2.
            
            Summary: The protein encoded by this gene is an RNA-binding protein
            that regulates pre-mRNA splicing, export of mRNAs from the nucleus,
            protein translation, and mRNA stability. The encoded protein is
            involved in myelinization and oligodendrocyte differentiation and
            may play a role in schizophrenia. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1; also known as HQK-5 and QKI-5).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB067798.1, SRR1803614.124586.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361752.8/ ENSP00000355094.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               AB067801.1         76-479
            405-1475            AF272349.1         362-1432
            1476-4588           AB067798.1         1552-4664
            4589-9341           AL031781.1         4352-9104           c
            9342-9384           AW131362.1         21-63               c
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q26"
     gene            1..9384
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="QKI, KH domain containing RNA binding"
                     /db_xref="GeneID:9444"
                     /db_xref="HGNC:HGNC:21100"
                     /db_xref="MIM:609590"
     exon            1..618
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             477..1502
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homolog of mouse quaking QKI (KH domain RNA binding
                     protein); RNA binding protein HQK; quaking homolog, KH
                     domain RNA binding; QKI/LOC100132735 fusion; KH
                     domain-containing RNA-binding protein QKI"
                     /codon_start=1
                     /product="KH domain-containing RNA-binding protein QKI
                     isoform 1"
                     /protein_id="NP_006766.1"
                     /db_xref="CCDS:CCDS5285.1"
                     /db_xref="GeneID:9444"
                     /db_xref="HGNC:HGNC:21100"
                     /db_xref="MIM:609590"
                     /translation="MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERL
                     LDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRI
                     LGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDA
                     QNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSL
                     AATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVP
                     PGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTA
                     DRAATGN"
     exon            619..761
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            762..878
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1022
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1110
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1410
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1485
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..9384
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4565..4570
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      4588
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="major polyA site"
     regulatory      4644..4649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      4656
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      7447..7452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: AATAAA"
     polyA_site      7468
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      9300..9305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: ATTAAA"
     polyA_site      9319
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
     regulatory      9360..9365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
                     /note="hexamer: ATTAAA"
     polyA_site      9384
                     /gene="QKI"
                     /gene_synonym="Hqk; hqkI; QK; QK1; QK3"
ORIGIN      
        1 gcagtcggag cgcggcggca gcggcagagg cgccgcggcg gggaccagcc cagagagacc
       61 ccccgagccc gcggcacagg cggcggcggc gctgagcggg ctcgaccagc cgagcgagcg
      121 gcggccccgg ctcctcctcg tccggcgcca gcagcccgcg ccccgcgccc gcactcggcc
      181 agccgagacc cgcctcccgc ccgcccgccg gcctcgtgag ggacgcgccg agccgggcgg
      241 ccgagagcgg cggcggctgg gagcgcgtcg ggcccgggcg gaaagtgcct gcggggggcg
      301 ggcgagcgcg cggtgccggc cgccccgggg ctcggcgcgg gagccagagc gggagccggc
      361 gcggagcggg acgccgggtc ccgagcggcc cgcggccggg gctcgccccc gcccctccct
      421 cctctccggc ggcggcggcg gcggcggcgg gcggagtgag ctgcggagcc tggaatatgg
      481 tcggggaaat ggaaacgaag gagaagccga agcccacccc agattacctg atgcagctga
      541 tgaacgacaa gaagctcatg agcagcctgc ccaacttctg cgggatcttc aaccacctcg
      601 agcggctgct ggacgaagaa attagcagag tacggaaaga catgtacaat gacacattaa
      661 atggcagtac agagaaaagg agtgcagaat tgcctgatgc tgtgggacct attgttcagt
      721 tacaagagaa actttatgtg cctgtaaaag aatacccaga ttttaatttt gttgggagaa
      781 tccttggacc tagaggactt acagccaaac aacttgaagc agaaaccgga tgtaaaatca
      841 tggtccgagg caaaggctca atgagggata aaaaaaagga ggagcaaaat agaggcaagc
      901 ccaattggga gcatctaaat gaagatttac atgtactaat cactgtggaa gatgctcaga
      961 acagagcaga aatcaaattg aagagagcag ttgaagaagt gaagaaatta ttggtacctg
     1021 cagcagaagg agaagacagc ctgaagaaga tgcagctgat ggagcttgcg attctgaatg
     1081 gcacctacag agatgccaac attaaatcac cagcccttgc cttttctctt gcagcaacag
     1141 cccaggctgc tccaaggatc attactgggc ctgcgccggt tctcccacca gctgccctgc
     1201 gtactcctac gccagctggc cctaccataa tgcctttgat cagacaaata cagaccgctg
     1261 tcatgccaaa cggaactcct cacccaactg ctgcaatagt tcctccaggg cccgaagctg
     1321 gtttaatcta tacaccctat gagtacccct acacattggc accagctaca tcaatccttg
     1381 agtatcctat tgaacctagt ggtgtattag gtgcggtggc tactaaagtt cgaaggcacg
     1441 atatgcgtgt ccatccttac caaaggattg tgaccgcaga ccgagccgcc accggcaact
     1501 aacctatgac cttctgacct ctgaactctt cacccaatga tgacctgacc atgcctgcct
     1561 gctgatcagt taactggtaa tcgcctttgc ttgcctgtcg tcagtgcagc gagctgaggc
     1621 acttgtccgt tcgtcttacc atctaaccaa acaaaagaca aagaaattgt tgtcctccaa
     1681 ctcagctttt tttttttttt tttcctgttt gggtgaaagt ggttctagaa actgcactga
     1741 atagtagtaa agcaataagg cccaattcat cccacagcac tgatcatctt ttaatatccc
     1801 accctaagcg aacggtaaga aggcctctct taagaagggg agacagatgg tccttaacta
     1861 ctcaatgaca gaggcagtta ctgtgagaga cttctaggaa tctttttctt ctcatagcga
     1921 agtcaaagct ctctctgaat gtactgtgtg atgatgcatc atgcatgaac cttcggtcag
     1981 ggatatcatt ggtgaagtga tttcaaaaag tattcaaaat ttgatatgct gtttagtcac
     2041 tacagtgccc tcaaagggca gaagttgcag ccttttttat attgcctgcc aaaatttgaa
     2101 gtattagaag aaagtgtgcc atgagagaaa aacttaagga gttttgaaaa gtaatgcaaa
     2161 taacaaaact gcaacactat ttttaaaaag ataaatatct gagttaaaat tactgaatct
     2221 ttattttaca cctaaaaaaa tatgagaaca aggtacatgc attatgtgtc acattactgg
     2281 gcaaactgtt caagtatttt tttttaaacc tccctgtata gaaaaaaatc attaaggatg
     2341 taaaagccat gcttgcctat ttgctgtata catgtaatga aattgtagat aaagtgtagt
     2401 gcattgaaac aaatgaacaa aaagtagata cttttactat acaagggtgc tggtgcagaa
     2461 aaaaatatat atatttttgg aaatgtagca ttttatactt tcaagtgtta taaaaaaaaa
     2521 gaaaaagaac aaagaaaccc tttatttcat taaatgattt tttctggtgg ttgtcaagaa
     2581 acaaaagacc aaaagagcct ttttgtcttt cttttttatt ttttaagtat tggaataagt
     2641 ctaaagaaaa gaaagtgcct tattttcctt catggtcatt cagtcaagtg tctcataaag
     2701 atcagctcct ccctcagaat acaagttaat gcatgttact cagtcgccca gtatgtgaaa
     2761 gaagatatga gggagacaaa tgagttgata gtttgaatta taggattttt gccacgttac
     2821 cttgatgcaa atttgccaaa tctgatactg tgaaaagatt tgataacttt tctaatgcct
     2881 gactagtaag ttttaaaaca tcattcttta aaagaatagt ttaatacttt tggttattcc
     2941 aatcttcaat gttaacccaa aggattctga gataaaaatt atgtgtcatc taaacttcag
     3001 tttacagaca aaattagagc tttgttttat ttggagaagg gggatgcatt catttgattt
     3061 tgttttttgt ttttaattaa ttgaatataa taggatacct agcaaatgtt ttcacaaaag
     3121 tgaatctttg tgattttcat tctagaaaac aatgttttaa atcacccaaa tttaatcaca
     3181 tgtctttata tcataccaga taactggtgt agtgtatcct tttactgttt caataacctc
     3241 tgcctccccc accccttaac tttaaaagaa tgtgaaaaac tagcatcata gtgcatataa
     3301 atactcaacc acatttcagc ttaagcttct aatttctcaa gaagttgaaa cagttagtta
     3361 tgtagttgaa gcaatttgtg tgcaaagcct acatctaaac acttgagtga ataatcatta
     3421 actgctcagt accagacgtg gcaaatctca agtgacagtg gacccccccc cccgcccagc
     3481 ttatcaactc gtcccctctt ccacaccttc ccagcatcac agtcagaggt cagcaggaag
     3541 ctgagtcatt accttcttgg tgacctcccc caccatcttg tctcacttgg aaaccgttgt
     3601 cagtttattt actatgcaat agacattcat tgttttgtat ccagctagct ttggtttaat
     3661 atgccactgc tttgtctttc tgttacacat acagttttga tttatttaca atatatgctg
     3721 tgccattttg atttttatat tgtttggttg gttgaataaa tttgcgacac tcaaacactt
     3781 gaggtgatag ttgttaaaaa caataccagt atttgatcca gtttcatctc aactatgtta
     3841 tacctggaca ttttagatgt ttatcaaagg tcttttatgg ggaagaaagt aaatgtatga
     3901 aataataaat gtgtgacatt tatagggaat gttggctctg aacaaacttt tgaaaacttg
     3961 gttgacaaaa ttttggatca actgaaaaaa gcatgacttt tgaaatctct gaatgccttg
     4021 gttctcagta ttatcattct ttattgaatt tatttcttat taaaatatgt agtttttaag
     4081 actttttttc tgacagtatt atgtaatttt ttagcgtggg tagatgggag tgtcgcttgt
     4141 atgttatcgt acagctgaca tgtatttttg tctattcttt attatcttag tttcatgcta
     4201 tgtatgtacc ataaccaacc tattgcctat gagaaacatg taagataatg tatttacagc
     4261 cattgttaca agtttataat gtatttttct atcttgtttt atatgtatgt tatataacat
     4321 tcaaaaagaa tttttttctt gattgagaaa aggatacaaa atgcaaaatc cacaattttg
     4381 ataactgaaa attgccaatt gttttgcagt actttattat attgggtgtc ttgtcttttt
     4441 tgggctttta gttagctaat gaatgaatac attagacact tttgggtttt agttgggatt
     4501 ttacatagct tgcattttaa ttctttggtt ctttgctgtt tctattaacc cacagcatta
     4561 ttttaataaa tgttataata ccaacctact aactctggac tttgtctgtt tattaacctt
     4621 tatatgttta attaaaataa acaaataaag acaaaagaat gtaaagtctt gagttactgg
     4681 actaaccctg aagaacgtga ccacagataa catagtgtga cttgttttta tgttgtttgt
     4741 cagctgacag ttttgcacac tactgttaaa atggctttgg ttattctggt cttttagctt
     4801 gggggccggg gcgggtgtgg gtgtaggtgc agtagagatg ggaacaaagt agcctttgtt
     4861 tgaggcattc ttctcttgcc acttgccttt cagcatctgc attactaatt ccacacctta
     4921 tttttccttc tccaaaataa tcactgtctg ctcacagcat cgagtaaaaa gatacattgt
     4981 catggtatat acctggagaa accagtttga ttctttgttg aaccatttct tttaatttct
     5041 gtaaataagt aattttatgg tcacattgta aagcagagaa actgttgata ttttaccttt
     5101 atttagattt ttgttttaac ttgacctttt tcctttggaa agcgttgaac ttgtttagct
     5161 aactactcct attaggggag cttaacttgg gtaaagtcaa aggcccactg gctgtttctc
     5221 atggtggcct gaagccccgc tgctttccag tgaggaaaag aaccctcagc acgttactga
     5281 gctttcctca ggtatttttt tctctgtcac ctactttaca aatacagtca gactaaaaca
     5341 ttaaacaaga agtttaaggg ggaataacat tgggatttga aaagttgtat gtgattaatt
     5401 tcttgcatgc aaatcaattt aagatttctt aaatatcttc atgcttttct tggctggaac
     5461 ttgggtaaat taacctgtaa ttatgaatta tttagtttaa ctcaaggtgc atttttattt
     5521 cacagattga tacttgcttt tttctgtgaa acagttctcc tagataccaa aatcctcagg
     5581 atgaattttt gcatttgtaa ataacaattt atctttgtaa attacatttt atttatttag
     5641 agttctaaaa ttgagggctg aaatgaatga catgtaatag aagacaaaac tgtaccaata
     5701 cgctgttaac caatcagact gtccaggtat tctcatttca taaatatgta tacacacata
     5761 taaatacaca gagtagtgtt agctgatgtg agtgataaca gcgaatcacc attgagatgg
     5821 tcatttggat catgtaaaca aaccgtgtca tgtctctgat gggagcggtg ggtagtgtac
     5881 tgttaggtgg acaggtcttt taacatgcta ctatttgtaa aagtttccgt tgccataaca
     5941 gttcatgtag tagtctgtga aaattcgaag ctgtctgtca agctgctaat atacaagttg
     6001 tcatggtacc atgtaactta aattgatgaa tttctatgca atttactatt ctctgaagcc
     6061 tatgaatgca gcctcatctc attttcagtt aagggtactt tatgtataat tatgtcattg
     6121 ctgcctttta tgtaggaaat gtaaaaaagt tttaaaaggc gaaaataaca ttacagattt
     6181 catttttata caggttacaa tattaaacat gaaactacca aattccaaga atagtaaaat
     6241 tctagagtca gttgattgat tgaaactatt tgacaaaaca gttgaaagct gagctttcag
     6301 tcaagaagtt ggtgaaatta atgtccttaa acttttcctt taggcaaaat gtcagggaaa
     6361 acttaacatg aaagtagtta ctcccatacg caccagtgca gtagctccag gtgtaagagg
     6421 tcacgaaggc accctgccca taaaaccagg atgtgcatac gtacacacaa tcggtgtctg
     6481 gttatggttt tctaaacact acataaggat ctgaattttt tagtgtgcca aaagcaaacg
     6541 ataggtttaa atgaaattgc tcactaccaa atcaagactc ttcatatata tacgtgtgtg
     6601 tatatatata tgtcataaca ctttcacgtg tgacagccta aggtttcttt agttttagga
     6661 tggggtgggg tgggagtggt tttgcatgtt tgaccaggat gtgatgcctg tctttattcc
     6721 ttgataagaa gtagctgtta cagcatttca agcaaattat tttaggtaga atagaactta
     6781 ctgccaaatg attatttatt tagcaaaaat tattctccaa cttttcgaat tcacaatttt
     6841 tctaagtgca tagagtagtt ttttacattg tcacgaattc cttaatacct ttatttaaaa
     6901 tggaaaaaat atggacaata ttttagaaat atgtgctaca catctaattt tatctgtagt
     6961 ttaagtgatc taaattgaga tgcctttgat atgaagagat ttaccaacat tatatgcact
     7021 cgtgccttca atgtggaatc aaactggtta acctcagcac agtcctctcg tttctgtgtt
     7081 tctgctgcac taattgccag ggggtggggg agaaggcgaa tgaattaatt catagtagaa
     7141 ggaggcgata ggtgcagcaa agggcagcca cagtgttgat tccacattat aatgttgttg
     7201 cctcttcttg gcaaaagaca ccacattgtg ggaagatctc agcttccagg gtaaaagtta
     7261 atttataact taaaagtgct attaagtttt tattaccaaa tatatctttt atggtttata
     7321 ttgtagtggt atgtatgaaa catttaaaat tttactgtgg aaattgtgta tatatatata
     7381 tatagtcgaa ataggtgttc acaggtcaca tgtgaacgga gaactgcatg accgtacatg
     7441 aaatgcaata aaccaactgg aaaaagtgca tgtgcttcat cctctcaagc caactgcagc
     7501 tggaaagtgc tgcttatcct ccacccccag aaaatgcatg tatcaatatg agaataaaga
     7561 acgcacactt tcaattttat tgaggctttc aacactattt aaaagaaaat gtaagaattt
     7621 gacattctgg agttattata acattagaaa atgagcataa cattcactct gattttagcc
     7681 attaagggag attagtaaac agactgctac agtgttccat agttggactg tgcatccaaa
     7741 acattttttt atctttaata aatggtacag tttttatgta gttttcgaat gtaagaagaa
     7801 aggaatgctg accaaaactt gatttcatca gcttcatgaa aaggactagt gtcattaacc
     7861 tgttgaacag aattggttta ttaaaaaaat catttccagt agtgtgaaac ctttacgagt
     7921 ctttaacatc taaatgttat gactccttgt accttaagtt ttccagtctt tcttatttat
     7981 atcatctcca agtacctctg gctcctttcc tcttgctcac cggaacctta gttttcctca
     8041 acagaatgct ttgttaaagt agcccacagt tgcaggatcc atagcaccgt cgtgcagact
     8101 agcagcccaa aggtgtgttt ggtttggctt atacggtgtt ttgcttttta aactacttgc
     8161 cataatttaa aagtggcaac actagactta aaaaaaaaaa agtctgattg cccatattag
     8221 attttttttt taattcttca caaaatctgc tcttcctgaa agatcaaagt gtctagaaag
     8281 cccaaacatg tattcttaac atagtaggca ccagctgaaa ctgagtaatt aaacggtccc
     8341 ctgaagccaa gtattccctg gttagtcgcc accccaccac tccttcctgt ctctagtgtc
     8401 acacttgggc tgttgatttt cttactcttc tcttgcttta atccttcccc ccggctctct
     8461 ggctcctgtg gatatctgtg cttgtttcct ggtccaggat ggtgatctga ctttcaaacc
     8521 agcttctcaa aaggggtgac ataaaatcag ttttgatgtt tttcctcctg aaaaatcaga
     8581 tgaatatttt agtcactgtt actcatgcac attgtgttct tatgtttaca gaagtgctta
     8641 agtgaatgga agcactagac atttggattt ccttcctaac gtaattttta atgattaccc
     8701 cttttataca gtaatttgtg gtctttagaa agcagttaaa ctaattgacc atctaatagt
     8761 tgtactatac atatgtctaa aataatagtc atggtaagtt tggcatcata tcttccccaa
     8821 aaaatgttta ttaaaattag attattccag tttaatgcta ttttgtgaac tgtataccct
     8881 ctgaaagcgc ttatttttac atgctacaca acagttccaa ttttaaggag tgtctcctaa
     8941 aattgggatg aaaatctact gtagtctgtt ttaaagtatg ctatactatg ttcattggtt
     9001 acttaactgg atattaatat aaaggttatt acaagaaaaa tgatgaagag caaaaggaga
     9061 agaaaatatt tgcaagtgaa tccacaattc ttgcagaact atttgagttg atactaaaga
     9121 ttttatgttc actcctttac cttagaactg tcaagcttgg gaatgaggaa agtgccttgg
     9181 ctgtgcttga aacctgaatt ttagtgcttc ccttattaca ttcattgttt tcaatgattg
     9241 atttataaaa ttaagacata ctggtagtac aagttgaaag ttggtttgaa tacattttaa
     9301 ttaaatgata atatgttaat atgcttttgt tcattgcttt ctcactgagg taaaacagca
     9361 ttaaaaagtt gtccagaatt taaa
//
