LOCUS       NM_002864               4635 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens PZP alpha-2-macroglobulin like (PZP), mRNA.
ACCESSION   NM_002864
VERSION     NM_002864.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Hu,Z., Zhang,M., Fan,J., Hu,J., Lin,G., Piao,S., Liu,P., Liu,J.,
            Fu,S., Sun,W., Gygi,S.P., Zhang,J. and Zhou,C.
  TITLE     High-Level Secretion of Pregnancy Zone Protein Is a Novel Biomarker
            of DNA Damage-Induced Senescence and Promotes Spontaneous
            Senescence
  JOURNAL   J Proteome Res 22 (11), 3570-3579 (2023)
   PUBMED   37831546
  REMARK    GeneRIF: High-Level Secretion of Pregnancy Zone Protein Is a Novel
            Biomarker of DNA Damage-Induced Senescence and Promotes Spontaneous
            Senescence.
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Lob,S., Vattai,A., Kuhn,C., Mittelberger,J., Herbert,S.L.,
            Wockel,A., Schmoeckel,E., Mahner,S. and Jeschke,U.
  TITLE     The Pregnancy Zone Protein (PZP) is significantly downregulated in
            the placenta of preeclampsia and HELLP syndrome patients
  JOURNAL   J Reprod Immunol 153, 103663 (2022)
   PUBMED   35843132
  REMARK    GeneRIF: The Pregnancy Zone Protein (PZP) is significantly
            downregulated in the placenta of preeclampsia and HELLP syndrome
            patients.
REFERENCE   3  (bases 1 to 4635)
  AUTHORS   Wu,M., Lan,H., Ye,Z. and Wang,Y.
  TITLE     Hypermethylation of the PZP gene is associated with hepatocellular
            carcinoma cell proliferation, invasion and migration
  JOURNAL   FEBS Open Bio 11 (3), 826-832 (2021)
   PUBMED   33471436
  REMARK    GeneRIF: Hypermethylation of the PZP gene is associated with
            hepatocellular carcinoma cell proliferation, invasion and
            migration.
REFERENCE   4  (bases 1 to 4635)
  AUTHORS   Lob,S., Vattai,A., Kuhn,C., Schmoeckel,E., Mahner,S., Wockel,A.,
            Kolben,T., Keil,C., Jeschke,U. and Vilsmaier,T.
  TITLE     Pregnancy Zone Protein (PZP) is significantly upregulated in the
            decidua of recurrent and spontaneous miscarriage and negatively
            correlated to Glycodelin A (GdA)
  JOURNAL   J Reprod Immunol 143, 103267 (2021)
   PUBMED   33388716
  REMARK    GeneRIF: Pregnancy Zone Protein (PZP) is significantly upregulated
            in the decidua of recurrent and spontaneous miscarriage and
            negatively correlated to Glycodelin A (GdA).
REFERENCE   5  (bases 1 to 4635)
  AUTHORS   Kumar,R., Kuligina,E., Sokolenko,A., Siddiqui,Q., Gardi,N.,
            Gupta,S., Varma,A.K. and Hasan,S.K.
  TITLE     Genetic ablation of pregnancy zone protein promotes breast cancer
            progression by activating TGF-beta/SMAD signaling
  JOURNAL   Breast Cancer Res Treat 185 (2), 317-330 (2021)
   PUBMED   33057846
  REMARK    GeneRIF: Genetic ablation of pregnancy zone protein promotes breast
            cancer progression by activating TGF-beta/SMAD signaling.
            Erratum:[Breast Cancer Res Treat. 2023 Jun;199(3):627-629. doi:
            10.1007/s10549-023-06939-7.. PMID: 37079214]
REFERENCE   6  (bases 1 to 4635)
  AUTHORS   Devriendt,K., Van den Berghe,H., Cassiman,J.J. and Marynen,P.
  TITLE     Primary structure of pregnancy zone protein. Molecular cloning of a
            full-length PZP cDNA clone by the polymerase chain reaction
  JOURNAL   Biochim Biophys Acta 1088 (1), 95-103 (1991)
   PUBMED   1989698
REFERENCE   7  (bases 1 to 4635)
  AUTHORS   Christensson,A., Laurell,C.B. and Lilja,H.
  TITLE     Enzymatic activity of prostate-specific antigen and its reactions
            with extracellular serine proteinase inhibitors
  JOURNAL   Eur J Biochem 194 (3), 755-763 (1990)
   PUBMED   1702714
REFERENCE   8  (bases 1 to 4635)
  AUTHORS   Marynen,P., Devriendt,K., Van den Berghe,H. and Cassiman,J.J.
  TITLE     A genetic polymorphism in a functional domain of human pregnancy
            zone protein: the bait region. Genomic structure of the bait
            domains of human pregnancy zone protein and alpha 2 macroglobulin
  JOURNAL   FEBS Lett 262 (2), 349-352 (1990)
   PUBMED   1692292
  REMARK    Erratum:[FEBS Lett 1990 Jun 18;266(1-2):195]
REFERENCE   9  (bases 1 to 4635)
  AUTHORS   Sottrup-Jensen,L. and Birkedal-Hansen,H.
  TITLE     Human fibroblast collagenase-alpha-macroglobulin interactions.
            Localization of cleavage sites in the bait regions of five
            mammalian alpha-macroglobulins
  JOURNAL   J Biol Chem 264 (1), 393-401 (1989)
   PUBMED   2462561
REFERENCE   10 (bases 1 to 4635)
  AUTHORS   Sand,O., Folkersen,J., Westergaard,J.G. and Sottrup-Jensen,L.
  TITLE     Characterization of human pregnancy zone protein. Comparison with
            human alpha 2-macroglobulin
  JOURNAL   J Biol Chem 260 (29), 15723-15735 (1985)
   PUBMED   2415522
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010175.4, X54380.1 and
            DB040351.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002864.2.
            
            Summary: The protein encoded by this gene is highly expressed in
            late-pregnancy serum and is similar in structure to
            alpha-2-macroglobulin. The encoded protein, which acts as a
            homotetramer, inhibits the activity of all four classes of
            proteinases. This protein contains cleavage sites for several
            proteinases. Upon binding of a proteinase, the conformation of this
            protein changes to trap the proteinase, limiting its activity. This
            protein appears to be elevated in the sera of presymptomatic
            Alzheimer's disease patients. [provided by RefSeq, Dec 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X54380.1, AK313639.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN04284274 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261336.7/ ENSP00000261336.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AC010175.4         61590-61614         c
            26-1370             X54380.1           1-1345
            1371-1371           AC010175.4         45896-45896         c
            1372-2390           X54380.1           1347-2365
            2391-2710           DB040351.1         261-580
            2711-2825           X54380.1           2686-2800
            2826-2826           AC010175.4         15832-15832         c
            2827-4121           X54380.1           2802-4096
            4122-4122           AC010175.4         6096-6096           c
            4123-4635           X54380.1           4098-4610
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..4635
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /note="PZP alpha-2-macroglobulin like"
                     /db_xref="GeneID:5858"
                     /db_xref="HGNC:HGNC:9750"
                     /db_xref="MIM:176420"
     exon            1..137
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..4503
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /note="Pregnancy zone protein; C3 and PZP-like
                     alpha-2-macroglobulin domain-containing protein 6;
                     pregnancy-zone protein"
                     /codon_start=1
                     /product="pregnancy zone protein precursor"
                     /protein_id="NP_002855.2"
                     /db_xref="CCDS:CCDS8600.1"
                     /db_xref="GeneID:5858"
                     /db_xref="HGNC:HGNC:9750"
                     /db_xref="MIM:176420"
                     /translation="MRKDRLLHLCLVLLLILLSASDSNSTEPQYMVLVPSLLHTEAPK
                     KGCVLLSHLNETVTVSASLESGRENRSLFTDLVAEKDLFHCVSFTLPRISASSEVAFL
                     SIQIKGPTQDFRKRNTVLVLNTQSLVFVQTDKPMYKPGQTVRFRVVSVDENFRPRNEL
                     IPLIYLENPRRNRIAQWQSLKLEAGINQLSFPLSSEPIQGSYRVVVQTESGGRIQHPF
                     TVEEFVLPKFEVKVQVPKIISIMDEKVNITVCGEYTYGKPVPGLATVSLCRKLSRVLN
                     CDKQEVCEEFSQQLNSNGCITQQVHTKMLQITNTGFEMKLRVEARIREEGTDLEVTAN
                     RISEITNIVSKLKFVKVDSHFRQGIPFFAQVLLVDGKGVPIPNKLFFISVNDANYYSN
                     ATTNEQGLAQFSINTTSISVNKLFVRVFTVHPNLCFHYSWVAEDHQGAQHTANRVFSL
                     SGSYIHLEPVAGTLPCGHTETITAHYTLNRQAMGELSELSFHYLIMAKGVIVRSGTHT
                     LPVESGDMKGSFALSFPVESDVAPIARMFIFAILPDGEVVGDSEKFEIENCLANKVDL
                     SFSPAQSPPASHAHLQVAAAPQSLCALRAVDQSVLLMKPEAELSVSSVYNLLTVKDLT
                     NFPDNVDQQEEEQGHCPRPFFIHNGAIYVPLSSNEADIYSFLKGMGLKVFTNSKIRKP
                     KSCSVIPSVSAGAVGQGYYGAGLGVVERPYVPQLGTYNVIPLNNEQSSGPVPETVRSY
                     FPETWIWELVAVNSSGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPF
                     FVELTMPYSVIRGEVFTLKATVLNYLPKCIRVSVQLKASPAFLASQNTKGEESYCICG
                     NERQTLSWTVTPKTLGNVNFSVSAEAMQSLELCGNEVVEVPEIKRKDTVIKTLLVEAE
                     GIEQEKTFSSMTCASGANVSEQLSLKLPSNVVKESARASFSVLGDILGSAMQNIQNLL
                     QMPYGCGEQNMVLFAPNIYVLNYLNETQQLTQEIKAKAVGYLITGYQRQLNYKHQDGS
                     YSTFGERYGRNQGNTWLTAFVLKTFAQARSYIFIDEAHITQSLTWLSQMQKDNGCFRS
                     SGSLLNNAIKGGVEDEATLSAYVTIALLEIPLPVTNPIVRNALFCLESAWNVAKEGTH
                     GSHVYTKALLAYAFSLLGKQNQNREILNSLDKEAVKEDNLVHWERPQRPKAPVGHLYQ
                     TQAPSAEVEMTSYVLLAYLTAQPAPTSGDLTSATNIVKWIMKQQNAQGGFSSTQDTVV
                     ALHALSRYGAATFTRTEKTAQVTVQDSQTFSTNFQVDNNNLLLLQQISLPELPGEYVI
                     TVTGERCVYLQTSMKYNILPEKEDSPFALKVQTVPQTCDGHKAHTSFQISLTISYTGN
                     RPASNMVIVDVKMVSGFIPLKPTVKMLERSSSVSRTEVSNNHVLIYVEQVTNQTLSFS
                     FMVLQDIPVGDLKPAIVKVYDYYETDESVVAEYIAPCSTDTEHGNV"
     exon            138..321
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            322..481
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            482..534
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            535..555
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            556..724
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            725..809
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            810..921
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1036
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1146
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1308
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1536
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1600
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1743
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1893
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2055
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2161
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2312
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2541
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2668
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2790
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2842
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..2926
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3103
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3104..3191
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3192..3348
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3349..3423
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3604
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3605..3828
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3829..4047
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4048..4175
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4176..4266
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4335
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4336..4438
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4439..4480
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4481..4635
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4609..4614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /note="hexamer: AATAAA"
     polyA_site      4635
                     /gene="PZP"
                     /gene_synonym="CPAMD6"
                     /note="major polyA site"
ORIGIN      
        1 agggcgaggc tgagggcaga gagttggaca caaccctgag atttatccct cacaatgcgg
       61 aaagacagac ttcttcattt atgtcttgtg ctacttctta tcctgctttc tgccagtgac
      121 tcaaactcta cagaaccgca gtatatggtg ctggtcccct ccctgctcca cactgaggcc
      181 cctaagaagg gctgtgtcct tctgagccac ctgaatgaga cagtgactgt aagtgcttcc
      241 ttggagtctg gcagggaaaa caggagcctc ttcactgacc tggtggcgga gaaggactta
      301 ttccactgtg tctccttcac tctcccaagg atctcagcct cttcagaggt ggcattcctt
      361 agcatccaga taaaggggcc tacgcaagat ttcaggaaga ggaacacagt tctggtactg
      421 aacacccaaa gtctggtctt tgtccagaca gacaaaccca tgtataaacc aggacagaca
      481 gtaagattcc gtgttgtctc cgtggatgaa aattttcgcc ctcgaaatga actgattcca
      541 ctgatatacc ttgagaaccc aagaagaaat cgaattgcac aatggcagag tctcaagcta
      601 gaagctggca tcaatcagtt gtcctttccc ctctcatcag agcccattca gggctcctac
      661 agggtggtgg tacagacaga atcaggtgga aggatacagc accccttcac cgtggaggaa
      721 tttgtgcttc ccaagtttga ggtcaaagtt caggtgccaa agataatcag tatcatggat
      781 gaaaaagtga acataacagt ctgtggagaa tacacttatg ggaagcctgt cccaggactt
      841 gcaactgtga gcctgtgtag aaaattatct cgtgttctta attgtgacaa gcaggaggtc
      901 tgtgaggaat tcagtcaaca gcttaacagc aatggctgca tcacccaaca agtacacacc
      961 aaaatgctcc agattacaaa tacgggcttt gaaatgaagc ttagagtgga agccaggatc
     1021 agagaagagg ggacagacct ggaagtcact gcaaacagga tcagtgaaat cacaaacatt
     1081 gtatccaaac tcaaattcgt gaaagtggat tcacacttta gacaaggaat cccctttttt
     1141 gcacaggtgc ttctggtgga tggaaaaggt gtgcccatcc ccaataaact cttcttcatc
     1201 tctgtgaatg acgccaatta ttactccaat gcaaccacca atgagcaggg tcttgcacag
     1261 ttttcaatca atactaccag tatctcggtt aataaacttt ttgtccgggt tttcactgtg
     1321 catcccaact tgtgttttca ctattcatgg gtagcagaag accaccaggg tgctcagcac
     1381 actgcaaatc gtgttttctc cttaagtgga agttacattc acctggagcc tgtggctggt
     1441 accctgccct gtggccacac ggagactatc acggcacact atacactgaa tagacaggcc
     1501 atgggagagt tatcggagct cagtttccat tacctgatca tggctaaggg agtcatcgtc
     1561 agatctggaa cccacactct gcctgtggag tcaggagaca tgaaaggcag ttttgcctta
     1621 tccttccctg tggagtcaga cgttgccccc attgcacgaa tgttcatctt tgccatttta
     1681 ccagatggag aagttgttgg agactctgaa aaatttgaga ttgaaaactg tctagccaac
     1741 aaggtggatt tgagcttcag cccagcacaa agtcccccag cctcacatgc ccacctgcaa
     1801 gtagcagctg ctccgcagtc cctctgtgcc cttcgtgctg tggaccaaag tgtgctgctc
     1861 atgaagcctg aggctgagct ctctgtgtcc tcagtatata atctgctaac tgtgaaggat
     1921 ctcaccaatt ttcctgacaa tgtggaccag caggaggaag aacaaggaca ctgtccccgt
     1981 cctttcttca ttcataatgg agccatctat gttcccttat caagtaatga agcagatatt
     2041 tatagcttcc tcaaggggat gggattgaag gtgttcacta actcaaaaat ccgaaaacca
     2101 aagtcgtgtt cagtcatccc ttccgtgtct gcaggagcag taggtcaagg atactatgga
     2161 gcaggtctag gagtagtaga gagaccatat gttcctcaat taggcacata taatgtgata
     2221 cccttaaata atgaacaaag ttcagggcca gtccctgaaa cggtgcgaag ctattttcct
     2281 gagacttgga tctgggagtt ggtggcagtg aactcatcag gtgtggctga ggtaggagta
     2341 acagtccctg acaccatcac cgagtggaag gcaggggcct tctgcctgtc cgaagatgct
     2401 ggacttggta tctcttccac tgcctctctc cgagccttcc agcccttctt tgtggagctc
     2461 acaatgcctt actctgtgat tcgtggagag gtcttcacac tcaaggccac ggtcctaaac
     2521 taccttccca aatgcatccg ggtcagtgtg cagctgaaag cctctccagc cttcctagct
     2581 tcccaaaata caaagggaga agaatcctat tgtatctgtg gaaatgagag acaaaccttg
     2641 tcttggacag tgactcctaa aactctgggg aatgtgaact tctcagtgag tgcagaggca
     2701 atgcagtcct tagaactctg tggaaatgag gttgttgagg tccctgagat taaaagaaaa
     2761 gacacagtca tcaaaaccct gttggtggag gctgaaggta ttgagcaaga aaagactttc
     2821 agttctatga cctgtgcctc aggtgctaat gtgtctgagc agttgtcctt gaagctccca
     2881 tcaaatgtgg tcaaagaatc tgccagagct tctttctcag ttctgggtga catattaggt
     2941 tctgctatgc aaaatataca aaatctcctc cagatgccat atggctgtgg agaacagaac
     3001 atggtcctat ttgctcctaa catctatgtc ttgaactatc tgaatgaaac ccagcagctg
     3061 acgcaggaga tcaaggccaa ggccgttggc tatctcatca ctggttacca gagacagctg
     3121 aactacaaac accaagatgg ctcctacagc acctttgggg aacgatatgg caggaaccag
     3181 ggcaacactt ggctcacagc ttttgtactg aagactttcg cccaggctcg atcctacatc
     3241 ttcattgatg aagcacacat tacccaatct ctcacgtggc tctcccagat gcagaaggac
     3301 aatggctgtt tcaggagctc tgggtcactg ctcaacaatg ccataaaggg aggtgtagaa
     3361 gatgaagcga ccctctccgc ctatgttact attgcccttc tggaaattcc tctcccagtc
     3421 actaacccta ttgttcgcaa tgccctgttc tgcctggagt cagcctggaa tgtagcaaag
     3481 gaggggaccc atgggagcca tgtctacacc aaggcattgc tggcctatgc tttttcccta
     3541 ctgggaaagc aaaatcagaa tagagaaata ctgaactcac ttgataagga agctgtgaaa
     3601 gaagacaacc tcgtccattg ggagcgccct cagagaccca aggcaccagt ggggcatctt
     3661 taccaaaccc aggctccctc tgctgaggtg gagatgacat cctatgtgct cctcgcttat
     3721 ctcacggccc agccagcccc cacctcaggg gacctgacct ctgcaactaa cattgtgaag
     3781 tggatcatga agcagcagaa cgcccaaggt ggtttctcct ccacccagga cacagtggtg
     3841 gctctccatg ccctgtccag gtatggagca gccactttca ccagaactga gaaaactgca
     3901 caggtcaccg ttcaggattc acagaccttt tctacaaatt tccaagtaga caacaacaac
     3961 ctcctattac tgcagcagat ctcattgcca gagctccctg gagaatatgt cataacagta
     4021 actggggaaa gatgtgtgta tcttcagaca tccatgaaat acaatattct tccagagaaa
     4081 gaggactccc catttgcttt aaaagtgcag actgtgcccc aaacttgcga tggacacaaa
     4141 gcccacacca gctttcagat ctcactgacc atcagttaca caggaaaccg tcctgcttcc
     4201 aatatggtga ttgttgatgt aaagatggta tctggtttta ttcccctgaa accaacagta
     4261 aaaatgcttg aaagatctag ctctgtgagc cggacagaag tgagcaacaa ccatgtcctc
     4321 atttatgtgg aacaggtgac aaatcagacg ctaagttttt ccttcatggt tctgcaagac
     4381 atcccagtag gagacttgaa gccagcaatt gttaaagtct atgattacta tgagacagat
     4441 gagtctgtgg ttgctgagta tatcgccccc tgcagcacag atacagagca tggaaatgtt
     4501 tgaggaccat acaggctgta tattttggtg gattctctgt cctatacatt tacttagaag
     4561 gaatggagtt atttgtctct ataaaataga cactaaaaat atttgctgaa taaatatgta
     4621 cttctggtca aacta
//
