LOCUS       NM_032906               1311 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens PIGY upstream open reading frame (PYURF), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_032906
VERSION     NM_032906.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Rensvold,J.W., Shishkova,E., Sverchkov,Y., Miller,I.J.,
            Cetinkaya,A., Pyle,A., Manicki,M., Brademan,D.R., Alanay,Y.,
            Raiman,J., Jochem,A., Hutchins,P.D., Peters,S.R., Linke,V.,
            Overmyer,K.A., Salome,A.Z., Hebert,A.S., Vincent,C.E.,
            Kwiecien,N.W., Rush,M.J.P., Westphall,M.S., Craven,M., Akarsu,N.A.,
            Taylor,R.W., Coon,J.J. and Pagliarini,D.J.
  TITLE     Defining mitochondrial protein functions through deep multiomic
            profiling
  JOURNAL   Nature 606 (7913), 382-388 (2022)
   PUBMED   35614220
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1311)
  AUTHORS   Murakami,Y., Siripanyaphinyo,U., Hong,Y., Tashima,Y., Maeda,Y. and
            Kinoshita,T.
  TITLE     The initial enzyme for glycosylphosphatidylinositol biosynthesis
            requires PIG-Y, a seventh component
  JOURNAL   Mol Biol Cell 16 (11), 5236-5246 (2005)
   PUBMED   16162815
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK312082.1, CV812938.1 and
            CB305903.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032906.4.
            
            Summary: The product of this gene, which is well-conserved, is
            encoded by the same bicistronic transcript that encodes
            phosphatidylinositol glycan anchor biosynthesis, class Y, but the
            two proteins are unrelated. This gene represents the protein
            encoded by the upstream open reading frame, while the protein
            encoded by the downstream open reading frame is represented by
            GeneID:84992. [provided by RefSeq, Aug 2012].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.603099.1,
                                           SRR3476690.914787.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            bicistronic transcript             :: PMID: 16162815
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000273968.5/
                                                  ENSP00000273968.4
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-421               AK312082.1         1-421
            422-609             CV812938.1         383-570
            610-1311            CB305903.1         39-740              c
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q22.1"
     gene            1..1311
                     /gene="PYURF"
                     /gene_synonym="NDUFAFQ; PREY"
                     /note="PIGY upstream open reading frame"
                     /db_xref="GeneID:100996939"
                     /db_xref="HGNC:HGNC:44317"
                     /db_xref="MIM:619956"
     exon            1..279
                     /gene="PYURF"
                     /gene_synonym="NDUFAFQ; PREY"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..421
                     /gene="PYURF"
                     /gene_synonym="NDUFAFQ; PREY"
                     /note="protein preY, mitochondrial; PIGY upstream reading
                     frame protein; NADH:ubiquinone oxidoreductase complex
                     assembly factor Q"
                     /codon_start=1
                     /product="protein preY, mitochondrial precursor"
                     /protein_id="NP_116295.1"
                     /db_xref="CCDS:CCDS3631.1"
                     /db_xref="GeneID:100996939"
                     /db_xref="HGNC:HGNC:44317"
                     /db_xref="MIM:619956"
                     /translation="MLSGARCRLASALRGTRAPPSAVARRCLHASGSRPLADRGKKTE
                     EPPRDFDPALLEFLVCPLSKKPLRYEASTNELINEELGIAYPIIDGIPNMIPQAARMT
                     RQSKKQEEVEQR"
     exon            280..1311
                     /gene="PYURF"
                     /gene_synonym="NDUFAFQ; PREY"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1277..1282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PYURF"
                     /gene_synonym="NDUFAFQ; PREY"
                     /note="hexamer: AATAAA"
     polyA_site      1311
                     /gene="PYURF"
                     /gene_synonym="NDUFAFQ; PREY"
                     /note="major polyA site"
ORIGIN      
        1 ctggacgaac gggaagccgg gagctcggcc acgggtggcg aggctgcggt gaggcctggt
       61 ctccggctgc cagaccatgc tgagtggagc acgctgcagg ctcgcctcag cgctgcgggg
      121 aacgcgcgcg ccgccgtccg cggtcgcccg taggtgcctg cacgcgtcgg ggtcgcggcc
      181 tttggccgac cggggcaaga agactgagga gccgccccgc gacttcgatc cggcgctgct
      241 ggagttcctg gtgtgcccgc tctccaagaa gccgctcaga tatgaagcat caacaaacga
      301 attgattaat gaagagttgg gaatagctta tccaatcatt gatgggatcc ctaatatgat
      361 accacaggca gctaggatga cacgtcaaag taagaagcaa gaagaagtgg agcagcgcta
      421 gttcataatt taaaaaaatt aaaaaaacgc aacagccaac ttttcttaat accatatacc
      481 ttttaaaaca cagtggcagg taataagtgg aagagaagaa tgtttctgtc tcttcctacg
      541 ttgactgttc ttattccact ggtttcttta gcaggactgt tctactcagc ctctgtggaa
      601 gaaaacttcc cacagggctg cactagcaca gccagccttt gcttttacag cctgctcttg
      661 cctattacca taccagtgta tgtattcttc cacctttgga cttggatggg tattaaactc
      721 ttcaggcata attgatgcaa ctagagtcaa tatgctgtat atattaatga tagctcttgg
      781 gcatcgatct ctgaaagctc aaatggatgg aatttagttt gcgggaaaga ggctttgctt
      841 tgcgcatatc aggcttagga ctgtgggagg cttaagttgc agatgcttct tttattgtac
      901 tcttgttctg cccttgtttt ttgaaggctc tgacttataa ctgctgtatc agaagaaaca
      961 ttttgacagt gtcttggttg gagatgaaca tccctaattg acatgtgatg actatttctt
     1021 attccattca tctaagagtc attgaaattt tgttttgctt gtttgtttag cttcaaggtc
     1081 tttggtaaag tcacatgtta aggatgactg aaataattcc aaaggagtga tgttggaata
     1141 gtccctctaa gggagagaaa tgcatttgaa cgaatgtgat ataaaaccac ataatcaaat
     1201 agaaacttca tgtacttaca aaaactgagt ttgtaaaatt accttcattt ctttgacatt
     1261 aaatgcttat attagcaata aacatgttga cactttccta taaaaaataa a
//
