LOCUS       NM_032345               1207 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens PYM homolog 1, exon junction complex associated factor
            (PYM1), transcript variant 1, mRNA.
ACCESSION   NM_032345
VERSION     NM_032345.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1207)
  AUTHORS   Ramage,H.R., Kumar,G.R., Verschueren,E., Johnson,J.R., Von
            Dollen,J., Johnson,T., Newton,B., Shah,P., Horner,J., Krogan,N.J.
            and Ott,M.
  TITLE     A combined proteomics/genomics approach links hepatitis C virus
            infection with nonsense-mediated mRNA decay
  JOURNAL   Mol Cell 57 (2), 329-340 (2015)
   PUBMED   25616068
REFERENCE   4  (bases 1 to 1207)
  AUTHORS   Singh,G., Kucukural,A., Cenik,C., Leszyk,J.D., Shaffer,S.A.,
            Weng,Z. and Moore,M.J.
  TITLE     The cellular EJC interactome reveals higher-order mRNP structure
            and an EJC-SR protein nexus
  JOURNAL   Cell 151 (4), 750-764 (2012)
   PUBMED   23084401
  REMARK    Erratum:[Cell. 2012 Nov 9;151(4):915-6]
REFERENCE   5  (bases 1 to 1207)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 1207)
  AUTHORS   Chamieh,H., Ballut,L., Bonneau,F. and Le Hir,H.
  TITLE     NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex
            and stimulate its RNA helicase activity
  JOURNAL   Nat Struct Mol Biol 15 (1), 85-93 (2008)
   PUBMED   18066079
REFERENCE   7  (bases 1 to 1207)
  AUTHORS   Diem,M.D., Chan,C.C., Younis,I. and Dreyfuss,G.
  TITLE     PYM binds the cytoplasmic exon-junction complex and ribosomes to
            enhance translation of spliced mRNAs
  JOURNAL   Nat Struct Mol Biol 14 (12), 1173-1179 (2007)
   PUBMED   18026120
  REMARK    GeneRIF: suggest that PYM functions as a bridge between EJC-bearing
            spliced mRNAs and the translation machinery to enhance translation
            of the mRNAs
REFERENCE   8  (bases 1 to 1207)
  AUTHORS   Gehring,N.H., Kunz,J.B., Neu-Yilik,G., Breit,S., Viegas,M.H.,
            Hentze,M.W. and Kulozik,A.E.
  TITLE     Exon-junction complex components specify distinct routes of
            nonsense-mediated mRNA decay with differential cofactor
            requirements
  JOURNAL   Mol Cell 20 (1), 65-75 (2005)
   PUBMED   16209946
REFERENCE   9  (bases 1 to 1207)
  AUTHORS   Forler,D., Kocher,T., Rode,M., Gentzel,M., Izaurralde,E. and
            Wilm,M.
  TITLE     An efficient protein complex purification method for functional
            proteomics in higher eukaryotes
  JOURNAL   Nat Biotechnol 21 (1), 89-92 (2003)
   PUBMED   12483225
REFERENCE   10 (bases 1 to 1207)
  AUTHORS   Gatfield,D. and Izaurralde,E.
  TITLE     REF1/Aly and the additional exon junction complex proteins are
            dispensable for nuclear mRNA export
  JOURNAL   J Cell Biol 159 (4), 579-588 (2002)
   PUBMED   12438415
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC025162.48 and AC023055.40.
            
            On Nov 23, 2018 this sequence version replaced NM_032345.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006135.1, SRR5189661.175290.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2147920
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000408946.7/ ENSP00000386156.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               AC025162.48        23825-23994         c
            171-264             AC023055.40        115533-115626       c
            265-1207            AC023055.40        113559-114501       c
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..1207
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /note="PYM homolog 1, exon junction complex associated
                     factor"
                     /db_xref="GeneID:84305"
                     /db_xref="HGNC:HGNC:30258"
                     /db_xref="MIM:619753"
     exon            1..170
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..748
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /note="isoform 1 is encoded by transcript variant 1;
                     partner of Y14 and mago; protein wibg homolog; within bgcn
                     homolog"
                     /codon_start=1
                     /product="partner of Y14 and mago isoform 1"
                     /protein_id="NP_115721.1"
                     /db_xref="CCDS:CCDS41795.1"
                     /db_xref="GeneID:84305"
                     /db_xref="HGNC:HGNC:30258"
                     /db_xref="MIM:619753"
                     /translation="MEAAGSPAATETGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPV
                     YENKYVKFFKSKPELPPGLSPEATAPVTPSRPEGGEPGLSKTAKRNLKRKEKRRQQQE
                     KGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLKKK
                     LRQVEELQQRIQAGEVSQPSKEQLEKLARRRALEEELEDLELGL"
     exon            171..264
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /inference="alignment:Splign:2.1.0"
     exon            265..1207
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1186..1191
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /note="hexamer: AATAAA"
     polyA_site      1207
                     /gene="PYM1"
                     /gene_synonym="PYM; WIBG"
                     /note="major polyA site"
ORIGIN      
        1 actcagggtt aggaggtctg ggcgagaagc aactagggcc ctcatcactt cgccgccgaa
       61 tccccggcgc cgcccagcgg ggcagagcca ggccagggcc gcccgcccaa cctggtccgc
      121 tgcctcttcg gccatggaag ctgccggcag ccctgcggct acggagacag gcaagtatat
      181 cgcgtcaaca cagcgacctg acgggacctg gcgcaagcag cggagggtga aagaaggata
      241 tgtgccccag gaggaggtcc cagtatatga aaacaagtat gtgaagtttt tcaagagtaa
      301 accagagttg cccccagggc taagccctga ggccactgct cctgtcaccc catccaggcc
      361 tgaaggtggt gaaccaggcc tctccaagac agccaaacgt aacctgaagc gaaaggagaa
      421 gaggcggcag cagcaagaga aaggagaggc agaggccttg agcaggactc ttgataaggt
      481 gtccctggaa gagacagccc aactccccag tgctccacag ggctctcggg cagcccccac
      541 agctgcatct gaccagcctg actcagctgc caccactgag aaagccaaga agataaagaa
      601 cctaaagaag aaactccggc aggtggaaga gctgcagcag cggatccagg ctggggaagt
      661 cagccagcct agcaaagagc agctagaaaa gctagcaagg aggagggcgc tagaagagga
      721 gttagaggac ttggagttag gcctctgagg cctttgggga atagggaatg gactgcagaa
      781 caaaccgtgg ggctctctgg ggtctggggg aatacgggca acagcagtca ggaggggtac
      841 cccccatact ggcttacttc cacctcctgc ggcccagctc tgtcctccag agcctagcgt
      901 ctccctcaat ccttcccttt tcttcccaac ttctactttt tggactttcc ccctcccatt
      961 cccagtgttc aaaatctcag tgactacccc aggtaccttt gctgctgatt tgggtgtctt
     1021 gtttaaaaga aaatcaggtg ggtgggaatc tcttggagaa ctgaggctga gggtagaggg
     1081 agtatgccca agtcttggag tcttggttcc tgttcgcggt gtttatgggt tatttccctc
     1141 tccatccctc attttttttt tttttttaaa aaaagcaaaa atgagaataa acacaagtag
     1201 acatgtc
//
