LOCUS       NM_023078               3342 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens pyrroline-5-carboxylate reductase 3 (PYCR3),
            transcript variant 1, mRNA.
ACCESSION   NM_023078
VERSION     NM_023078.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Zhuang,F., Huang,S. and Liu,L.
  TITLE     PYCR3 modulates mtDNA copy number to drive proliferation and
            doxorubicin resistance in triple-negative breast cancer
  JOURNAL   Int J Biochem Cell Biol 171, 106581 (2024)
   PUBMED   38642827
  REMARK    GeneRIF: PYCR3 modulates mtDNA copy number to drive proliferation
            and doxorubicin resistance in triple-negative breast cancer.
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Meeks,K.R. and Tanner,J.J.
  TITLE     Expression and kinetic characterization of PYCR3
  JOURNAL   Arch Biochem Biophys 733, 109468 (2023)
   PUBMED   36414121
REFERENCE   3  (bases 1 to 3342)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3342)
  AUTHORS   Kirin,M., Chandra,A., Charteris,D.G., Hayward,C., Campbell,S.,
            Celap,I., Bencic,G., Vatavuk,Z., Kirac,I., Richards,A.J.,
            Tenesa,A., Snead,M.P., Fleck,B.W., Singh,J., Harsum,S.,
            Maclaren,R.E., den Hollander,A.I., Dunlop,M.G., Hoyng,C.B.,
            Wright,A.F., Campbell,H., Vitart,V. and Mitry,D.
  TITLE     Genome-wide association study identifies genetic risk underlying
            primary rhegmatogenous retinal detachment
  JOURNAL   Hum Mol Genet 22 (15), 3174-3185 (2013)
   PUBMED   23585552
REFERENCE   5  (bases 1 to 3342)
  AUTHORS   De Ingeniis,J., Ratnikov,B., Richardson,A.D., Scott,D.A.,
            Aza-Blanc,P., De,S.K., Kazanov,M., Pellecchia,M., Ronai,Z.,
            Osterman,A.L. and Smith,J.W.
  TITLE     Functional specialization in proline biosynthesis of melanoma
  JOURNAL   PLoS One 7 (9), e45190 (2012)
   PUBMED   23024808
REFERENCE   6  (bases 1 to 3342)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   7  (bases 1 to 3342)
  AUTHORS   Merrill,M.J., Yeh,G.C. and Phang,J.M.
  TITLE     Purified human erythrocyte pyrroline-5-carboxylate reductase.
            Preferential oxidation of NADPH
  JOURNAL   J Biol Chem 264 (16), 9352-9358 (1989)
   PUBMED   2722838
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA629534.1, BC007993.1 and
            AC067930.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_023078.5.
            
            Summary: This gene encodes a protein that belongs to the
            pyrroline-5-carboxylate reductase family of enzymes. Members of
            this family catalyze the final step in proline biosynthesis,
            converting pyrroline-5-carboxylate to proline. Glutamate and
            ornithine are precursors in the synthesis of proline. The protein
            encoded by this gene is a cytoplasmic enzyme involved in the
            biosynthesis of proline from ornithine. [provided by RefSeq, Aug
            2016].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.29245.1,
                                           SRR1803612.51706.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000495276.6/ ENSP00000480945.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-536               DA629534.1         38-573
            537-1141            BC007993.1         556-1160
            1142-3342           AC067930.7         141375-143575       c
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..3342
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /note="pyrroline-5-carboxylate reductase 3"
                     /db_xref="GeneID:65263"
                     /db_xref="HGNC:HGNC:25846"
                     /db_xref="MIM:616408"
     exon            1..118
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..852
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /EC_number="1.5.1.2"
                     /note="isoform 1 is encoded by transcript variant 1; P5CR
                     3; P5C reductase 3; pyrroline-5-carboxylate
                     reductase-like"
                     /codon_start=1
                     /product="pyrroline-5-carboxylate reductase 3 isoform 1"
                     /protein_id="NP_075566.3"
                     /db_xref="CCDS:CCDS6407.3"
                     /db_xref="GeneID:65263"
                     /db_xref="HGNC:HGNC:25846"
                     /db_xref="MIM:616408"
                     /translation="MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPT
                     DRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVA
                     AGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETKLLQHLLEACG
                     RCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAK
                     MLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK"
     exon            119..183
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /inference="alignment:Splign:2.1.0"
     exon            184..363
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /inference="alignment:Splign:2.1.0"
     exon            364..576
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /inference="alignment:Splign:2.1.0"
     exon            577..669
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /inference="alignment:Splign:2.1.0"
     exon            670..3342
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2295..2300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /note="hexamer: AATAAA"
     polyA_site      2314
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /note="major polyA site"
     regulatory      2482..2487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /note="hexamer: AATACA"
     polyA_site      2499
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
     polyA_site      3216
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
     regulatory      3307..3312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
                     /note="hexamer: AATAAG"
     polyA_site      3342
                     /gene="PYCR3"
                     /gene_synonym="PYCRL"
ORIGIN      
        1 gagcgcagcg gcgtccgagg caacaagatg gcagctgcgg agccgtctcc gcggcgcgtg
       61 ggcttcgtgg gcgcgggccg catggcgggg gccatcgcgc agggcctcat cagagcagga
      121 aaagtggaag ctcagcacat actggccagt gcaccaacag acaggaacct atgtcacttt
      181 caagctctgg gttgccggac cacgcactcc aaccaggagg tgctgcagag ctgcctgctc
      241 gtcatctttg ccaccaagcc tcatgtgctg ccagctgtcc tggcagaggt ggctcctgtg
      301 gtcaccactg aacacatctt ggtgtccgtg gctgctgggg tgtctctgag caccctggag
      361 gagctgctgc ccccaaacac acgggtgctg cgggtcttgc ccaacctgcc ctgtgtggtc
      421 caggaagggg ccatagtgat ggcgcggggc cgccacgtgg ggagcagcga gaccaagctc
      481 ctgcagcatc tgctggaggc ctgtgggcgg tgtgaggagg tgcctgaagc ctacgtcgac
      541 atccacactg gcctcagtgg cagtggcgtg gccttcgtgt gtgcattctc cgaggccctg
      601 gctgaaggag ccgtcaagat gggcatgccc agcagcctgg cccaccgcat cgctgcccag
      661 accctgctgg ggacggccaa gatgctgctg cacgagggcc aacacccagc ccagctgcgc
      721 tcagacgtgt gcaccccggg tggcaccacc atctatggac tccacgccct ggagcagggc
      781 gggctgcgag cagccaccat gagcgccgtg gaggctgcca cctgccgggc caaggagctc
      841 agcagaaagt aggctgggct ctggccatcc tttcctgcct ctgtgcccct gcctctccct
      901 gtgtcccttc ccctgaggac tgcggctccc tccctcctgc atgagggtct cctactgctc
      961 cttctcccct tgcacaggga aatgcagggg gcaggacttg ggaggttcca gcaggcgggg
     1021 gagccccgac cagtggggac actcctccct ccccagtgag cagaaggcac cgtggtggtg
     1081 gctctgcccc ttgctgcagt gagcccacct tgctgcaaca ttggttctga ggggcccaag
     1141 agatggcgtc ttggtcattt gcccgcatgg ttgggcagtt ggttgaggcc atgaacagaa
     1201 cttacggtaa caggcacggc tggcccaatg cctggtctgg agctggagct tgcctttggc
     1261 tttccaggtg gctccgtgca gctacagcca ggccggctgc ctcatctcag ctctaggggg
     1321 cacgagccat atggggtctg cacaagagac cctctcccct gcagtaaagc caggggccct
     1381 ggcctgatgg ggcccccatg gggagctgga gcctgccctg cagcctggag aagagggtgg
     1441 ctgtggtggg cgtgctcatc ccctgctaag gagcaggagc tgctgggcca ggtctgcggc
     1501 agtgctgggg tggcaccagg tgggcagtgg taggtggggt ggcttgaggt ctgggagggt
     1561 ggccctggcc agccaggaca catgcagacc cctggcttta gtctggatac aggctccctc
     1621 tttcctccca atcctaagct cctgacaagt ggccaggtgg ctctgggccc tcctgccccg
     1681 tgcctaggtc aggggtcctg gaataccccg tagctctggc accaccacac tggcctctga
     1741 tggcaagact tggcccctcc acctgtccct aacggacggc aggtcaggaa agccaggact
     1801 caggggagaa gcaaaccccc aggattgaag gctagggttc tagggccttt gggtggggag
     1861 ggcccgggcc ggacagcctc agctccgtcc cctgccccac aagattcacc tgggcctcca
     1921 gtcccacgct ggccccaact gctgcagctc tcggcttccg cccaacagcc tctggaggtg
     1981 aggcgggagc atgccctcag cgaggctggg cggcgggtcc tgctgtgcca tctccctgtg
     2041 cgcctgagca gatcaatcca ccagtgcaaa acagggctaa cggcacctgc aggacagcag
     2101 cacgctccat ccctcatgct cagctgcctc tgcggccacg gacttctgcc cttcatctgc
     2161 tctctcttac tctcctgagc ctagcccgtc cgtaagctcc ctcccctgcc tggttcccag
     2221 ggcaggctga ctcagttgac tgcttggtcc aagcctggcc ctggcacttg tcagggtcag
     2281 cctaaggaga tgggaataaa gaggccagag agcaccaagt gagctcatgt ttcaccagag
     2341 gtggataaaa ccaagttctg ggctgggccc agtggctcac atctgtaatt ccagcacttt
     2401 gggaggccga ggcgggcaga tcacaaagtc aggagtttga gaccaacctg accaacatgg
     2461 agaaacccca tctctactaa aaatacaaaa ttagctggat gtggtggtgc gtgcctgtaa
     2521 tcccagctac ttgggaggct gaggcaggag aattgcttga atccaggagg cggagattgc
     2581 agagagccaa gatcatgcca ctgcactcca gcctgggtga cagcaagact ctgtcttgga
     2641 aaaaaaaaaa aagaaggtcc aatgcctgcc ccataagttg aggcctggaa ccttggtcaa
     2701 gcacaggtgt cagccgaagg gggtgctggc actgtggaca gcagaggtct ccccatgcct
     2761 gctacaggcc tctacatcac ctgggggctt tgtagggcat ggaattgcag tttcccctgc
     2821 acgctgtcac cccaggggac tgtatgccaa gcctgggcag gcctgggagc ctggggtctg
     2881 gtgcaggcgg ccccctgaag gaaggtggac agcccttctg acggccacct gcattcacac
     2941 caagcccatc tgcaaggaga aatcattgac tttgcaccaa cacctgtctc cccctgggac
     3001 ctctgacaag ccctgggcac taggtgagct cagctcggga ggtggagatc ccaagccagc
     3061 cactcctggc ccagctggtg agggcctgtg acaaagacac gggctcagtt ctcggcacgg
     3121 cagggagcgt tgtggtgagc caggcctccc ctgtgccttc tacttccagg tgaaaactgt
     3181 tcttcaagac agaaaacgat ggatatgatt acggtatctt tgatcaatgt tccactttta
     3241 tgccctatgc cccaactgtt aatctagggg gtattcctga tccaagcgtt ggatcaagag
     3301 tttaaaaata agacttcaaa acagggatgc tcatgaagtg ga
//
