LOCUS       NM_138499               2615 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens PWWP domain containing 2B (PWWP2B), transcript variant
            1, mRNA.
ACCESSION   NM_138499 XM_096329
VERSION     NM_138499.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ju,M.K., Lee,J.R., Choi,Y., Park,S.Y., Sul,H.J., Chung,H.J., An,S.,
            Lee,S., Jung,E., Kim,B., Choi,B.Y., Kim,B.J., Kim,H.S., Lim,H.,
            Kang,H.S., Soh,J.S., Myung,K., Kim,K.C., Cho,J.W., Seo,J.,
            Kim,T.M., Lee,J.Y., Kim,Y., Kim,H. and Zang,D.Y.
  TITLE     PWWP2B promotes DNA end resection and homologous recombination
  JOURNAL   EMBO Rep 23 (7), e53492 (2022)
   PUBMED   35582821
  REMARK    GeneRIF: PWWP2B promotes DNA end resection and homologous
            recombination.
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2615)
  AUTHORS   Zhang,T., Wei,G., Millard,C.J., Fischer,R., Konietzny,R.,
            Kessler,B.M., Schwabe,J.W.R. and Brockdorff,N.
  TITLE     A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to
            regulate transcription at active genes
  JOURNAL   Nat Commun 9 (1), 3798 (2018)
   PUBMED   30228260
  REMARK    GeneRIF: Study in HeLa, HEK293F, and mouse embryonic stem cells
            reveal that loss of PWWP2A/B leads to increases in histone
            acetylation predominantly at highly expressed genes, accompanied by
            decreases in Pol II elongation. Collectively, these findings
            suggest a role for PWWP2A/B in regulating transcription through the
            fine-tuning of histone acetylation dynamics at actively transcribed
            genes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2615)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   5  (bases 1 to 2615)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB477789.1, BC068574.1 and AW166547.1.
            
            On Nov 24, 2018 this sequence version replaced NM_138499.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068574.1, ERR3218379.699185.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305233.6/ ENSP00000306324.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DB477789.1         33-40
            9-291               BC068574.1         9-291
            292-319             AW166547.1         191-218             c
            320-2615            BC068574.1         320-2615
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.3"
     gene            1..2615
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /note="PWWP domain containing 2B"
                     /db_xref="GeneID:170394"
                     /db_xref="HGNC:HGNC:25150"
     exon            1..152
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1800
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /note="isoform 1 is encoded by transcript variant 1; PWWP
                     domain containing 2; PWWP domain-containing protein 2B"
                     /codon_start=1
                     /product="PWWP domain-containing protein 2B isoform 1"
                     /protein_id="NP_612508.3"
                     /db_xref="CCDS:CCDS7667.2"
                     /db_xref="GeneID:170394"
                     /db_xref="HGNC:HGNC:25150"
                     /translation="MEPRAGCRLPVRVEQVVNGALVVTVSCGERSFAGILLDCTKKSG
                     LFGLPPLAPLPQVDESPVNDSHGRAPEEGDAEVMQLGSSSPPPARGVQPPETTRPEPP
                     PPLVPPLPAGSLPPYPPYFEGAPFPHPLWLRDTYKLWVPQPPPRTIKRTRRRLSRNRD
                     PGRLILSTIRLRPRQVLCEKCKSTLSPPEASPGPPAAPRARRRLGSGPDRELRKPEEP
                     ENGEPTAAATARRSKRERREEDRAPAEQVPRSPVIKISYSTPQGKGEVVKIPSRVHGS
                     LEPFRPQQAPQDDGSQDPEVLDRESRDRPSCAPSASIPKLKLTRPVPAGADLPPPKIR
                     LKPHRLGDSEHEPVYRAELVGELNGYLRDSSPAPCADGPAGGLADLSSGSSGEDDDFK
                     SCPQGPQGREGLAFLVSCPEGRADCASESACSSDSLDEARSSGSEGTPADTGDLSPGH
                     GASAPSVSREARQTVPPLTVRLHTQSVSECITEDGRTVAVGDIVWGKIHGFPWWPARV
                     LDISLGQKEDGEPSWREAKVSWFGSPTTSFLSISKLSPFSEFFKLRFNRKKKGMYRKA
                     ITEAANAARHVAPEIRELLTQFET"
     exon            153..1816
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..2615
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2597..2602
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /note="hexamer: AAGAAA"
     polyA_site      2615
                     /gene="PWWP2B"
                     /gene_synonym="bA432J24.1; pp8607; PWWP2"
                     /note="major polyA site"
ORIGIN      
        1 gggggcggcc tgggacgcgg cgggagcatg gagccgcgcg ccggctgccg gctgccggtg
       61 cgggtggagc aggtcgtcaa cggcgcgctg gtggtcacgg tgagctgcgg cgagcggagc
      121 ttcgcgggga tcctgctgga ctgcacgaaa aagtccggcc tctttggcct acccccgttg
      181 gctccgctgc cccaggtcga tgagtcccct gtcaacgaca gccatggccg ggctcccgag
      241 gagggggatg cagaggtgat gcagctgggg tccagctccc cccctcctgc ccgcggggtt
      301 cagccccccg agaccacccg ccccgagcca cccccgcccc tcgtgccgcc gctgcccgcc
      361 ggaagcctgc ccccgtaccc tccctacttc gaaggcgccc ccttccctca cccgctgtgg
      421 ctccgggaca cgtacaagct gtgggtgccc cagccgccgc ccaggaccat caagcgcacg
      481 cggcggcgtc tgtcccgcaa ccgcgacccg gggcgcctca tcctcagcac catccgcctg
      541 cggccgcgcc aggtgctctg tgagaagtgc aagagcacgc tgagcccccc ggaggccagc
      601 cccggacccc cagccgcgcc cagggcccgc aggaggctgg gcagcggccc ggacagggag
      661 ctccgcaagc cggaggagcc ggagaacggc gagcccacgg ctgcggccac cgccaggagg
      721 agcaagaggg agaggcgcga ggaggacagg gccccggcag agcaggtccc gcggagcccg
      781 gtcatcaaga tctcctacag cacgccccag ggcaagggag aggtggtcaa gatcccctcc
      841 cgcgtgcacg gctctctgga gcccttccgt ccccagcagg ccccgcagga cgacggcagc
      901 caggaccccg aggtgctgga cagagagtcc cgggaccggc cgtcctgcgc gccctcggcc
      961 tccatcccca agttgaaact gacacggcct gtgccggccg gcgcggacct gccgccccct
     1021 aagatccgcc tgaagcccca ccgtctgggg gacagcgagc acgagcccgt gtaccgggcc
     1081 gagctggtgg gggagctgaa cgggtacctg cgggacagct cgccggcgcc ctgtgcggac
     1141 ggccctgccg gtgggctggc ggacttgtct tctggaagtt cgggtgagga cgatgacttc
     1201 aagagctgtc cccagggtcc acagggacgc gagggcttgg cttttctcgt cagctgccct
     1261 gaggggagag cggactgtgc cagtgagtcg gcgtgcagca gcgacagcct ggacgaggcc
     1321 agatcgtccg gctcggaagg gacgccggca gacacgggtg acctctcgcc tggccacggc
     1381 gcgtcagcgc cctcggtgtc cagagaggct cgccaaacgg tgccgcccct gacggtcagg
     1441 ctgcacacac agagcgtgtc ggagtgcatc acggaggacg gcaggactgt ggccgtgggg
     1501 gacatcgtct ggggtaagat ccatggtttt ccttggtggc cggcgcgtgt tcttgacatc
     1561 agtctcggcc agaaggagga cggagagccg tcttggcgag aagcgaaggt ctcgtggttt
     1621 ggttctccga ctacgtcgtt cttgtctatt tcaaaactct cccctttctc tgaatttttc
     1681 aaactgagat ttaatcgtaa gaagaagggg atgtatcgga aagctataac cgaggctgca
     1741 aatgccgcaa gacacgtggc cccggaaatc agggagctct taacccagtt tgaaacgtaa
     1801 ctggttccct gaccaggtca ccgacgatga gggcgcacct gctgtcctgg agcttccgat
     1861 ctctgttcgg ggaccctgtg agccttctgg ccggctgcgt gcagagccca ctgggcacgg
     1921 tggtcggcct ggtgtgaggc cccccgggga ccggcagtgt gtccagggag gggatggccc
     1981 tgagcccagc ggctcctccc gctgagtgta tttcttccca ccactcagcg catggctgcc
     2041 ctggctcacg aggcagtcgg gactcgtgac ttgctggctg ctggctgctg ctgcctcgcg
     2101 cgctggggtt ccatggagga actgggcctg ccgccccggc ctcacctgct gcccgcatgc
     2161 tggggtccca tggaggaacc aggcctggcg ccccggcctc gcccagcggc tggtgtgggc
     2221 agctgttccc tgcctcgcag tgtcctggag gcagctgtct cgcagactca gcttggtctc
     2281 ccgcaggctt cagaaaaacc caattgcacg tgtgggattc ttccccagcc cttgggtgcg
     2341 gggtctctgt gcacttgaga gctgggggac ccacccaccg tctcccactc caagttcatc
     2401 ccagagcgtg gggcccgctg ggcacctggc acctggcctg cagatgctgc tacgagtgac
     2461 cagtgctgtg tggaggaggc caagggcggc ccctgggaaa ccgggcttca acagtacaag
     2521 gaaaagaaac ttgctctgtt ttgtaagagc gtgttccttt tcctcttttt tgtcacttta
     2581 aagaccaaaa agaataaaga aagaaaagaa aaaaa
//
