LOCUS       NM_001130864            3373 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens PWWP domain containing 2A (PWWP2A), transcript variant
            2, mRNA.
ACCESSION   NM_001130864
VERSION     NM_001130864.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3373)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3373)
  AUTHORS   Hong,H., Sun,Y., Deng,H., Yuan,K., Chen,J., Liu,W. and Cui,Z.
  TITLE     Dysregulation of cPWWP2A-miR-579 axis mediates
            dexamethasone-induced cytotoxicity in human osteoblasts
  JOURNAL   Biochem Biophys Res Commun 517 (3), 491-498 (2019)
   PUBMED   31376935
  REMARK    GeneRIF: It has been shown that dysregulation of the circular RNA
            PWWP2A-miR-579 axis is involved in dexamethasone-induced
            cytotoxicity in human osteoblasts.
REFERENCE   5  (bases 1 to 3373)
  AUTHORS   Link,S., Spitzer,R.M.M., Sana,M., Torrado,M., Volker-Albert,M.C.,
            Keilhauer,E.C., Burgold,T., Punzeler,S., Low,J.K.K., Lindstrom,I.,
            Nist,A., Regnard,C., Stiewe,T., Hendrich,B., Imhof,A., Mann,M.,
            Mackay,J.P., Bartkuhn,M. and Hake,S.B.
  TITLE     PWWP2A binds distinct chromatin moieties and interacts with an
            MTA1-specific core NuRD complex
  JOURNAL   Nat Commun 9 (1), 4300 (2018)
   PUBMED   30327463
  REMARK    GeneRIF: PWWP2A is a complex chromatin-binding protein that serves
            to direct the deacetylase complex M1HR to H2A.Z-containing
            chromatin, thereby promoting changes in histone acetylation
            levels.PWWP2A's internal region binds H2A.Z nucleosomes and DNA.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3373)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   7  (bases 1 to 3373)
  AUTHORS   Zhang,T., Wei,G., Millard,C.J., Fischer,R., Konietzny,R.,
            Kessler,B.M., Schwabe,J.W.R. and Brockdorff,N.
  TITLE     A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to
            regulate transcription at active genes
  JOURNAL   Nat Commun 9 (1), 3798 (2018)
   PUBMED   30228260
  REMARK    GeneRIF: Study in HeLa, HEK293F, and mouse embryonic stem cells
            reveal that loss of PWWP2A/B leads to increases in histone
            acetylation predominantly at highly expressed genes, accompanied by
            decreases in Pol II elongation. Collectively, these findings
            suggest a role for PWWP2A/B in regulating transcription through the
            fine-tuning of histone acetylation dynamics at actively transcribed
            genes.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3373)
  AUTHORS   Punzeler,S., Link,S., Wagner,G., Keilhauer,E.C., Kronbeck,N.,
            Spitzer,R.M., Leidescher,S., Markaki,Y., Mentele,E., Regnard,C.,
            Schneider,K., Takahashi,D., Kusakabe,M., Vardabasso,C., Zink,L.M.,
            Straub,T., Bernstein,E., Harata,M., Leonhardt,H., Mann,M.,
            Rupp,R.A. and Hake,S.B.
  TITLE     Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural
            crest differentiation
  JOURNAL   EMBO J 36 (15), 2263-2279 (2017)
   PUBMED   28645917
  REMARK    GeneRIF: Thus, PWWP2A is a novel H2A.Z-specific multivalent
            chromatin binder providing a surprising link between H2A.Z,
            chromosome segregation, and organ development.
REFERENCE   9  (bases 1 to 3373)
  AUTHORS   Hein,M.Y., Hubner,N.C., Poser,I., Cox,J., Nagaraj,N., Toyoda,Y.,
            Gak,I.A., Weisswange,I., Mansfeld,J., Buchholz,F., Hyman,A.A. and
            Mann,M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   10 (bases 1 to 3373)
  AUTHORS   Joshi,P., Greco,T.M., Guise,A.J., Luo,Y., Yu,F., Nesvizhskii,A.I.
            and Cristea,I.M.
  TITLE     The functional interactome landscape of the human histone
            deacetylase family
  JOURNAL   Mol Syst Biol 9, 672 (2013)
   PUBMED   23752268
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC008706.5, DB450981.1, BC105110.1, AK055921.1, AI972512.1 and
            BC018680.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001130864.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            at its 3'-terminal exon, compared to variant 1, which results in a
            protein (isoform B) with a longer and distinct C-terminus, compared
            to isoform A.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.18035.1,
                                           SRR3476690.851419.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000307063.9/ ENSP00000305151.7
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AC008706.5         61998-62002
            6-28                DB450981.1         6-28
            29-1611             BC105110.1         1-1583
            1612-2682           AK055921.1         832-1902
            2683-3278           AI972512.1         41-636              c
            3279-3373           BC018680.1         1250-1344
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.3"
     gene            1..3373
                     /gene="PWWP2A"
                     /gene_synonym="MST101"
                     /note="PWWP domain containing 2A"
                     /db_xref="GeneID:114825"
                     /db_xref="HGNC:HGNC:29406"
                     /db_xref="MIM:617823"
     exon            1..646
                     /gene="PWWP2A"
                     /gene_synonym="MST101"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..2330
                     /gene="PWWP2A"
                     /gene_synonym="MST101"
                     /note="isoform b is encoded by transcript variant 2; PWWP
                     domain-containing protein 2A; MSTP101"
                     /codon_start=1
                     /product="PWWP domain-containing protein 2A isoform b"
                     /protein_id="NP_001124336.1"
                     /db_xref="CCDS:CCDS47332.1"
                     /db_xref="GeneID:114825"
                     /db_xref="HGNC:HGNC:29406"
                     /db_xref="MIM:617823"
                     /translation="MAAVAAEAAATAASPGEGGAGEAEPEMEPIPGSEAGTDPLPVTA
                     TEASVPDGETDGQQSAPQADEPPLPPPPPPPGELARSPEAVGPELEAEEKLSVRVAES
                     AAAAPQGGPELPPSPASPPEQPPAPEEREEPPLPQPVAPALVPPAGGDSTVSQLIPGS
                     EVRVTLDHIIEDALVVSFRFGEKLFSGVLMDLSKRFGPHGIPVTVFPKREYKDKPEAM
                     PLQSNTFQEGTEVKCEANGAVPDDPSPVPHPELSLAESLWTSKPPPLFHEGAPYPPPL
                     FIRDTYNQSIPQPPPRKIKRPKRKMYREEPTSIMNAIKLRPRQVLCDKCKNSVVAEKK
                     EIRKGSSATDSSKYEDKKRRNESVTTVNKKLKTDHKVDGKNQNESQKRNAVVKVSNIA
                     HSRGRVVKVSAQANTSKAQLSTKKVLQSKNMDHAKAREVLKIAKEKAQKKQNETSTSK
                     NAHSKVHFTRRYQNPSSGSLPPRVRLKPQRYRNEENDSSLKTGLEKMRSGKMAPKPQS
                     RCTSTRSAGEAPSENQSPSKGPEEASSEVQDTNEVHVPGDQDEPQTLGKKGSKNNISV
                     YMTLNQKKSDSSSASVCSIDSTDDLKSSNSECSSSESFDFPPGSMHAPSTSSTSSSSK
                     EEKKLSNSLKMKVFSKNVSKCVTPDGRTICVGDIVWAKIYGFPWWPARILTITVSRKD
                     NGLLVRQEARISWFGSPTTSFLALSQLSPFLENFQSRFNKKRKGLYRKAITEAAKAAK
                     QLTPEVRALLTQFET"
     exon            647..3373
                     /gene="PWWP2A"
                     /gene_synonym="MST101"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3352..3357
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWWP2A"
                     /gene_synonym="MST101"
                     /note="hexamer: AATAAA"
     polyA_site      3373
                     /gene="PWWP2A"
                     /gene_synonym="MST101"
                     /note="major polyA site"
ORIGIN      
        1 aagcagcgct gtcgccgccg ccgctgcagc cggagttgga ggagggagaa gctaggaaga
       61 aaatggcggc cgtggctgca gaggcggcag cgactgcagc gtcccccggg gaggggggcg
      121 ccggcgaggc cgagccggag atggagccca tccccggcag tgaggccggc actgaccccc
      181 tcccggtcac ggccactgaa gcgtctgtgc cggatggcga gactgacggg cagcaatccg
      241 ctcctcaggc cgacgagccg ccgctcccgc cgccaccgcc gccgccgggg gagctcgccc
      301 gcagcccaga ggcggtgggg ccggagctgg aggctgagga gaaactgtcc gttcgggtgg
      361 cggagtcggc ggcagccgcg cctcagggag ggccggaact tccaccttct cctgcatcgc
      421 cgccggagca gcccccggct cccgaggagc gcgaggagcc gccgctgcct cagcccgtag
      481 ccccggcgct cgtgccgccg gcgggcgggg actccacggt gtcgcaactg atcccgggct
      541 cggaggtgcg ggtcacgctg gaccacatca ttgaggacgc gcttgtcgtg tcgttccgct
      601 tcggggagaa gctcttctcc ggggtcctca tggatctgtc caaaaggttt gggccccatg
      661 gtatccctgt gacagtattt cccaaaaggg aatataagga taaaccagaa gccatgccgc
      721 tccaaagtaa tacattccaa gaagggacag aagtcaagtg tgaagcaaat ggtgctgttc
      781 ccgatgaccc ttctcctgtc ccgcatcccg agctgagctt ggctgaaagc ctgtggactt
      841 ccaaaccacc acctctcttc catgaaggag caccttatcc tccccctttg tttatcaggg
      901 acacatataa ccaatcaata cctcagccac ctcctcggaa aattaagcga cccaaacgaa
      961 aaatgtacag ggaagaaccc acttcaataa tgaatgctat taaactacga cccaggcaag
     1021 ttctgtgtga taaatgtaaa aacagtgttg ttgctgaaaa aaaggaaatt agaaaaggta
     1081 gtagtgcaac tgactcttct aaatatgaag ataaaaaacg gagaaatgaa agtgtaacta
     1141 ctgtgaacaa aaaactgaaa actgaccata aagtggatgg gaaaaaccaa aatgaaagcc
     1201 agaaaagaaa tgctgtggtt aaggtttcaa atattgctca cagcagaggc agagtagtaa
     1261 aagtttctgc tcaggcaaat acatcaaaag ctcagttaag tactaaaaaa gttctccaga
     1321 gtaagaacat ggatcatgcg aaagctcggg aagtgttaaa aattgccaaa gaaaaggcac
     1381 aaaagaagca aaatgaaacc tctacttcca aaaatgcaca ttcaaaagtc catttcacac
     1441 gtcgatatca gaatcctagc tcaggttccc ttccaccccg ggttcgttta aaaccacaga
     1501 ggtacaggaa tgaagaaaat gactcttctc tgaagacagg acttgagaaa atgcggagtg
     1561 gcaagatggc acccaagccc cagtctcgct gcacctctac ccgctcagca ggtgaggccc
     1621 cttcagaaaa tcagagtccc tcaaaaggcc ctgaagaggc cagcagtgag gttcaggaca
     1681 caaatgaagt gcatgtgcct ggtgatcagg atgaaccaca gacattgggc aaaaagggca
     1741 gcaaaaacaa tatctctgtt tatatgaccc taaatcaaaa gaaatctgac tcttccagtg
     1801 cttcagtgtg tagcattgat agcacagatg atttgaaatc ttccaactct gagtgtagtt
     1861 cttctgaaag ctttgatttt cctccaggca gtatgcatgc accttccacc tcctccactt
     1921 cctcctcttc aaaggaagag aaaaagctca gtaattcctt gaaaatgaaa gtcttttcca
     1981 aaaacgtctc taaatgcgtc acaccagatg gcaggaccat atgtgtaggg gacattgttt
     2041 gggccaagat atatggcttc ccttggtggc cagcccgtat tcttactata actgtgagcc
     2101 ggaaagataa cggcctttta gtccgacagg aggcccgtat ttcatggttt gggtctccaa
     2161 caacatcttt ccttgctctt tcacaactct cccccttttt agaaaacttc cagtcacgct
     2221 ttaataagaa gagaaagggc ctgtatcgca aggctatcac agaggcagct aaggctgcca
     2281 agcagctgac ccccgaagtg cgggctttgt tgacacagtt tgaaacgtga acatgggcag
     2341 taaggtaggc aagaccattg gaagtcacca cagattttct agtctagtta ggaataattt
     2401 ctacaaaata gcgtggccag attggagaga gaagttgcac tcagttggct ggctttttaa
     2461 tacttacctt atagccattt ttagactgag agcttaaact gaacatataa tcaaattttg
     2521 tgttaaggaa gtgagatttt agcagtattt ttcagttttg aagttcgaaa ccatcccaag
     2581 gcataggagc catagcctca actgaaattg aatttttgta gggactgtta attgccattt
     2641 gtacctaata ctgtatatat acatatatat acgtgtgtgt gtgtgtatat atatatatcc
     2701 gtgtatgtat atatacacac atatatatgt atatatacac atatatatat atgaaataca
     2761 gcctgtcact gtgtgacaca gattgcatat ttgggattgc aataagggct tggtgagctg
     2821 ggggaatagt ggatatttaa tgactacttg ttttttaaat aatgaacact tagcctttct
     2881 atgcgcataa tggtgtaaaa gttagatggt taggtgtttg acaaacagat ggttgcaatt
     2941 cagttacaga ctggggaaat agcccagtgt ttgggattaa aactgtcaag actggatagg
     3001 tgttagtcat cactgtcttt cgtccagtgg ggatttaata aatactttca agcttctggt
     3061 ttgggagttt agataggttt gattttggat ttgtaaatag tgattgctaa atttggtcag
     3121 atttctcctg actggctgtt cctaaattct taggatatgt cccagtaaaa ttatactttg
     3181 tgaattaatt gtcatatgtg taattgttgc attttggatg tacctaattt gataattaaa
     3241 acaaaataat ttccgtttaa ggtatctgtt tgcaggtcag aaaatgagaa aatgtaattg
     3301 tgtaatgctc tgaaatgtaa aaaaaaaaaa aaaaaccaaa aaaaagctgt aaataaaatc
     3361 aactaaatcc aaa
//
