LOCUS       NM_005049               3188 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens PWP2 small subunit processome component (PWP2), mRNA.
ACCESSION   NM_005049
VERSION     NM_005049.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Singh,S., Vanden Broeck,A., Miller,L., Chaker-Margot,M. and
            Klinge,S.
  TITLE     Nucleolar maturation of the human small subunit processome
  JOURNAL   Science 373 (6560), eabj5338 (2021)
   PUBMED   34516797
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3188)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   4  (bases 1 to 3188)
  AUTHORS   Nagamine,K., Kudoh,J., Minoshima,S., Kawasaki,K., Asakawa,S.,
            Ito,F. and Shimizu,N.
  TITLE     Genomic organization and complete nucleotide sequence of the human
            PWP2 gene on chromosome 21
  JOURNAL   Genomics 42 (3), 528-531 (1997)
   PUBMED   9205129
REFERENCE   5  (bases 1 to 3188)
  AUTHORS   Nagamine,K., Kudoh,J., Kawasaki,K., Minoshima,S., Asakawa,S.,
            Ito,F. and Shimizu,N.
  TITLE     Genomic organization and complete nucleotide sequence of the TMEM1
            gene on human chromosome 21q22.3
  JOURNAL   Biochem Biophys Res Commun 235 (1), 185-190 (1997)
   PUBMED   9196060
REFERENCE   6  (bases 1 to 3188)
  AUTHORS   Lafreniere,R.G., Rochefort,D.L., Chretien,N., Neville,C.E.,
            Korneluk,R.G., Zuo,L., Wei,Y., Lichter,J. and Rouleau,G.A.
  TITLE     Isolation and genomic structure of a human homolog of the yeast
            periodic tryptophan protein 2 (PWP2) gene mapping to 21q22.3
  JOURNAL   Genome Res 6 (12), 1216-1226 (1996)
   PUBMED   8973917
REFERENCE   7  (bases 1 to 3188)
  AUTHORS   Lalioti,M.D., Chen,H., Rossier,C., Shafaatian,R., Reid,J.D. and
            Antonarakis,S.E.
  TITLE     Cloning the cDNA of human PWP2, which encodes a protein with WD
            repeats and maps to 21q22.3
  JOURNAL   Genomics 35 (2), 321-327 (1996)
   PUBMED   8661145
REFERENCE   8  (bases 1 to 3188)
  AUTHORS   Yamakawa,K., Gao,D.Q. and Korenberg,J.R.
  TITLE     A periodic tryptophan protein 2 gene homologue (PWP2H) in the
            candidate region of progressive myoclonus epilepsy on 21q22.3
  JOURNAL   Cytogenet Cell Genet 74 (1-2), 140-145 (1996)
   PUBMED   8893822
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB001517.1 and KF510567.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005049.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.158677.1,
                                           SRR1803617.100273.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291576.12/ ENSP00000291576.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AB001517.1         6444-6479
            37-149              AB001517.1         8029-8141
            150-244             AB001517.1         12798-12892
            245-344             AB001517.1         13224-13323
            345-488             AB001517.1         13670-13813
            489-624             AB001517.1         14309-14444
            625-854             AB001517.1         14736-14965
            855-998             AB001517.1         16865-17008
            999-1069            AB001517.1         17808-17878
            1070-1206           AB001517.1         18432-18568
            1207-1355           AB001517.1         19393-19541
            1356-1503           AB001517.1         19676-19823
            1504-1654           AB001517.1         19997-20147
            1655-1835           AB001517.1         21222-21402
            1836-1983           AB001517.1         23625-23772
            1984-2092           AB001517.1         25056-25164
            2093-2158           AB001517.1         25945-26010
            2159-2377           AB001517.1         26977-27195
            2378-2528           AB001517.1         27292-27442
            2529-2603           AB001517.1         27597-27671
            2604-2773           KF510567.1         189-358
            2774-3188           AB001517.1         29813-30227
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..3188
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /note="PWP2 small subunit processome component"
                     /db_xref="GeneID:5822"
                     /db_xref="HGNC:HGNC:9711"
                     /db_xref="MIM:601475"
     exon            1..36
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..2778
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /note="PWP2 periodic tryptophan protein homolog; PWP2,
                     UTPB SSU processome subunit"
                     /codon_start=1
                     /product="periodic tryptophan protein 2 homolog"
                     /protein_id="NP_005040.2"
                     /db_xref="CCDS:CCDS33579.1"
                     /db_xref="GeneID:5822"
                     /db_xref="HGNC:HGNC:9711"
                     /db_xref="MIM:601475"
                     /translation="MKFAYRFSNLLGTVYRRGNLNFTCDGNSVISPVGNRVTVFDLKN
                     NKSDTLPLATRYNVKCVGLSPDGRLAIIVDEGGDALLVSLVCRSVLHHFHFKGSVHSV
                     SFSPDGRKFVVTKGNIAQMYHAPGKKREFNAFVLDKTYFGPYDETTCIDWTDDSRCFV
                     VGSKDMSTWVFGAERWDNLIYYALGGHKDAIVACFFESNSLDLYSLSQDGVLCMWQCD
                     TPPEGLRLKPPAGWKADLLQREEEEEEEEDQEGDRETTIRGKATPAEEEKTGKVKYSR
                     LAKYFFNKEGDFNNLTAAAFHKKSHLLVTGFASGIFHLHELPEFNLIHSLSISDQSIA
                     SVAINSSGDWIAFGCSGLGQLLVWEWQSESYVLKQQGHFNSMVALAYSPDGQYIVTGG
                     DDGKVKVWNTLSGFCFVTFTEHSSGVTGVTFTATGYVVVTSSMDGTVRAFDLHRYRNF
                     RTFTSPRPTQFSCVAVDASGEIVSAGAQDSFEIFVWSMQTGRLLDVLSGHEGPISGLC
                     FNPMKSVLASASWDKTVRLWDMFDSWRTKETLALTSDALAVTFRPDGAELAVATLNSQ
                     ITFWDPENAVQTGSIEGRHDLKTGRKELDKITAKHAAKGKAFTALCYSADGHSILAGG
                     MSKFVCIYHVREQILMKRFEISCNLSLDAMEEFLNRRKMTEFGNLALIDQDAGQEDGV
                     AIPLPGVRKGDMSSRHFKPEIRVTSLRFSPTGRCWAATTTEGLLIYSLDTRVLFDPFE
                     LDTSVTPGRVREALRQQDFTRAILMALRLNESKLVQEALEAVPRGEIEVVTSSLPELY
                     VEKVLEFLASSFEVSRHLEFYLLWTHKLLMLHGQKLKSRAGTLLPVIQFLQKSIQRHL
                     DDLSKLCSWNHYNMQYALAVSKQRGTKRSLDPLGSEEEAEASEDDSLHLLGGGGRDSE
                     EEMLA"
     exon            37..149
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            150..244
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            245..344
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            345..488
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            489..624
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            625..854
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            855..998
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1069
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1206
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1355
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1503
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1654
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1835
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1983
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2092
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2158
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2159..2377
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2528
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2603
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..3188
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3168..3173
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /note="hexamer: ATTAAA"
     polyA_site      3188
                     /gene="PWP2"
                     /gene_synonym="EHOC-17; PWP2H; UTP1"
                     /note="major polyA site"
ORIGIN      
        1 acacgtgcga cggccgtgat gaagttcgct taccggtttt caaatttgct gggtacggtg
       61 taccggcgtg ggaacctaaa ttttacctgc gatggaaatt cagttatcag tcccgtgggc
      121 aatagagtca ctgtatttga ccttaaaaac aacaaatctg acacgctgcc cctggccact
      181 cggtacaacg tcaagtgcgt ggggctgtcc ccggatggcc gcctcgctat catcgtcgat
      241 gaagggggcg atgcgctgct ggtcagcctg gtctgcaggt ctgtgctgca ccacttccac
      301 ttcaagggct ctgtgcacag tgtgtccttc tcccctgatg gcaggaagtt tgttgtcaca
      361 aagggtaaca ttgcccagat gtatcatgcc cctgggaaga agcgggagtt caacgccttc
      421 gttctggaca agacctattt tgggccctac gatgagacca cctgcatcga ctggacggat
      481 gactccaggt gctttgtggt tgggagcaaa gacatgtcca cctgggtgtt cggagccgag
      541 cgctgggaca acctcatcta ctatgcactg gggggacata aggatgccat cgtggcctgc
      601 ttctttgaat ccaacagcct ggacctgtac tcactcagcc aggacggagt gctgtgcatg
      661 tggcagtgtg acacgccccc cgagggcttg cggctgaagc cccctgcggg ctggaaagca
      721 gacctgttgc agcgggagga ggaagaggag gaggaggagg accaggaggg cgacagagag
      781 accaccatcc ggggaaaagc cactccggcc gaggaggaga agacaggaaa agtgaagtac
      841 tcacggctgg ccaagtactt cttcaataaa gaaggggatt ttaacaacct gacagctgca
      901 gcatttcata agaagtctca cctcttggtc actggctttg cttctggaat cttccatctt
      961 catgagctgc cagagtttaa cctcatccac tccctgagca tttcagatca gagcatcgcc
     1021 tcagtggcca tcaatagctc gggggactgg attgcttttg gctgttcagg cctgggccag
     1081 ctgctggtgt gggagtggca gagtgagtcc tacgtgctca agcagcaggg ccacttcaac
     1141 agcatggtgg ccctggccta ctcgcccgac ggacagtaca tcgtgactgg cggggacgac
     1201 ggcaaggtca aggtgtggaa caccctcagc ggcttctgct tcgtcacttt tacggagcac
     1261 tccagcgggg tgaccggtgt gacctttact gccaccggct acgttgtggt gacctcatcc
     1321 atggacggga ccgtgcgagc ctttgacctt cacaggtacc gaaacttccg caccttcacc
     1381 tctccacgcc ccacccagtt ctcctgtgtg gcggtggatg cgagcggtga gatcgtctct
     1441 gcaggggcgc aggactcctt tgagattttc gtgtggtcca tgcagacagg caggctcctt
     1501 gatgttttgt ctggacacga agggcccatc agtggtctgt gttttaaccc aatgaagtcc
     1561 gtcctggcca gtgcctcctg ggacaagaca gtgcgcctat gggacatgtt tgacagctgg
     1621 aggaccaagg agacgctggc cctgacctct gatgctctgg ctgtgacttt tcgccctgat
     1681 ggtgcggagc tggctgtggc cacactgaac tcacagatca ccttctggga ccctgagaac
     1741 gcggtgcaga cgggctccat tgagggcagg catgacctca agactggcag gaaggagctg
     1801 gacaagatta cagccaagca cgcggccaag gggaaggcct tcaccgccct gtgctactct
     1861 gcagacggcc acagcatcct ggcgggaggc atgtccaagt tcgtgtgcat ctaccacgtc
     1921 cgtgagcaga ttctcatgaa gaggttcgag atctcttgca acctgtcttt ggacgccatg
     1981 gaggaatttt tgaaccgaag aaaaatgaca gagtttggca acctggcact aattgatcag
     2041 gatgctgggc aggaggatgg agtcgcgata ccactgccag gcgtcaggaa aggtgacatg
     2101 agttctcggc acttcaaacc tgagatcagg gtgacctcac tccgcttctc tcccactggg
     2161 cgctgctggg cggccaccac cacggaggga ctcctcatct actccctgga cacccgcgtg
     2221 ctctttgacc cgtttgagct ggacaccagc gtcacccccg ggagggtgcg cgaggcactg
     2281 cgccagcagg acttcaccag ggccatcctc atggccctcc ggctcaacga gagcaaactg
     2341 gtgcaggagg ccctggaggc ggtgcccagg ggcgagattg aagtggtcac ctcctccctt
     2401 cctgaactgt atgtggagaa agtgctggag tttttagctt cctcctttga agtgtctcgc
     2461 cacctggaat tctacctcct ctggactcac aaactgctca tgttgcacgg acagaagctg
     2521 aagtccagag ccgggacgct gctgcctgtc attcagttcc tccagaagag catccagcgg
     2581 cacctggacg acctgtcgaa actctgtagc tggaaccact ataacatgca gtacgcacta
     2641 gcagtttcca agcagcgggg cacaaaacgc tccctagacc cgctgggaag tgaggaggag
     2701 gcagaagcat ctgaagatga cagcctgcat ctgcttggag gaggaggcag agactcagaa
     2761 gaagagatgc tggcctagag ccagccggtt gcagcgttgg attgtgccgg ctaagacctg
     2821 ccagggagat gggacccttg tgccacctgg gccagcaaag aggaggggtc cagagaacag
     2881 ctgaaatact gtcactagtg gtagtgactt gcttttcctg tgcacacatg tagcccatca
     2941 ggacagcgag ccgacgggtc acgccagggg ccggcacgca ctggcacctg gccccaggag
     3001 cggggccgtg tgaacggtga tgaatgttga aaatgcgtct cagagaggta ttcacatgaa
     3061 ctttgtatga gacttattta tatctttaac ataaaggttt gataaagaac ttagggatta
     3121 aaaaaaagat ggagttttct aacgtgagga tgaagtctac acttcagatt aaaaagggtt
     3181 atgttgta
//
