LOCUS       NM_007062               2549 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens PWP1 homolog, endonuclein (PWP1), transcript variant
            1, mRNA.
ACCESSION   NM_007062
VERSION     NM_007062.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Liu,Y., Mattila,J., Ventela,S., Yadav,L., Zhang,W., Lamichane,N.,
            Sundstrom,J., Kauko,O., Grenman,R., Varjosalo,M., Westermarck,J.
            and Hietakangas,V.
  TITLE     PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar
            Regulation of Ribosomal Gene Expression
  JOURNAL   Dev Cell 43 (2), 240-252 (2017)
   PUBMED   29065309
  REMARK    GeneRIF: High PWP1 expression is associated with head and neck
            squamous cell carcinoma.
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Ludvigsen,M., Ostergaard,M., Vorum,H., Jacobsen,C. and Honore,B.
  TITLE     Identification and characterization of endonuclein binding
            proteins: evidence of modulatory effects on signal transduction and
            chaperone activity
  JOURNAL   BMC Biochem 10, 34 (2009)
   PUBMED   20028516
  REMARK    GeneRIF: Endonuclein interacts with several proteins indicating a
            broad function including signal transduction and chaperone activity
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2549)
  AUTHORS   Honore,B., Baandrup,U., Nielsen,S. and Vorum,H.
  TITLE     Endonuclein is a cell cycle regulated WD-repeat protein that is
            up-regulated in adenocarcinoma of the pancreas
  JOURNAL   Oncogene 21 (7), 1123-1129 (2002)
   PUBMED   11850830
REFERENCE   4  (bases 1 to 2549)
  AUTHORS   Honore,B., Leffers,H., Madsen,P. and Celis,J.E.
  TITLE     Cloning of a cDNA encoding a novel human nuclear phosphoprotein
            belonging to the WD-40 family
  JOURNAL   Gene 151 (1-2), 291-296 (1994)
   PUBMED   7828893
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC380488.1, L07758.1,
            AC007622.28 and CA449439.1.
            
            On Nov 23, 2018 this sequence version replaced NM_007062.2.
            
            Summary: The protein encoded by this gene contains several WD-40
            repeats and is found mostly in the nucleus. The expression and
            localization of this protein are cell cycle dependent. Expression
            of this gene is upregulated in pancreatic adenocarcinoma. Three
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.94992.1,
                                           SRR1660803.118476.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000412830.8/ ENSP00000387365.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                DC380488.1         68-81
            15-1867             L07758.1           1-1853
            1868-2525           AC007622.28        79146-79803         c
            2526-2549           CA449439.1         16-39               c
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..2549
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="PWP1 homolog, endonuclein"
                     /db_xref="GeneID:11137"
                     /db_xref="HGNC:HGNC:17015"
                     /db_xref="MIM:620055"
     exon            1..173
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1607
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nuclear phosphoprotein similar to S. cerevisiae PWP1;
                     periodic tryptophan protein 1 homolog; keratinocyte
                     protein IEF SSP 9502; endonuclein"
                     /codon_start=1
                     /product="periodic tryptophan protein 1 homolog isoform 1"
                     /protein_id="NP_008993.1"
                     /db_xref="CCDS:CCDS9114.1"
                     /db_xref="GeneID:11137"
                     /db_xref="HGNC:HGNC:17015"
                     /db_xref="MIM:620055"
                     /translation="MNRSRQVTCVAWVRCGVAKETPDKVELSKEEVKRLIAEAKEKLQ
                     EEGGGSDEEETGSPSEDGMQSARTQARPREPLEDGDPEDDRTLDDDELAEYDLDKYDE
                     EGDPDAETLGESLLGLTVYGSNDQDPYVTLKDTEQYEREDFLIKPSDNLIVCGRAEQD
                     QCNLEVHVYNQEEDSFYVHHDILLSAYPLSVEWLNFDPSPDDSTGNYIAVGNMTPVIE
                     VWDLDIVDSLEPVFTLGSKLSKKKKKKGKKSSSAEGHTDAVLDLSWNKLIRNVLASAS
                     ADNTVILWDMSLGKPAASLAVHTDKVQTLQFHPFEAQTLISGSYDKSVALYDCRSPDE
                     SHRMWRFSGQIERVTWNHFSPCHFLASTDDGFVYNLDARSDKPIFTLNAHNDEISGLD
                     LSSQIKGCLVTASADKYVKIWDILGDRPSLVHSRDMKMGVLFCSSCCPDLPFIYAFGG
                     QKEGLRVWDISTVSSVNEAFGRRERLVLGSARNSSISGPFGSRSSDTPMES"
     exon            174..232
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            233..420
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            421..506
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            507..603
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            604..714
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            715..845
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            846..907
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1004
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1066
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1178
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1269
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1391
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1497
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..2549
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1831..1836
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="hexamer: AATAAA"
     polyA_site      1855
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="major polyA site"
     regulatory      2530..2535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="hexamer: ATTAAA"
     polyA_site      2549
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
ORIGIN      
        1 agcggccctg tgcagatccc tgagcgtgtg gcagcagtgc ggtcgtggtc cctccctatg
       61 cagcctggtt tctagcgtga cacgcccttg acttgaggac catgaaccgc agccgccagg
      121 tgacgtgcgt ggcctgggtc cgctgcggcg tggccaaaga gacaccagac aaggtagagc
      181 tgagtaaaga agaagtaaaa cgcctcattg ctgaggcaaa ggagaaattg caagaagaag
      241 gtggtggcag tgatgaagag gagacaggca gtccttcaga agatggcatg cagagtgcac
      301 gcacccaggc acgcccaaga gagcccctgg aggatggtga cccagaggat gacaggacgc
      361 ttgatgatga tgagctggct gagtacgact tagataaata tgatgaggaa ggtgacccag
      421 atgctgagac tcttggtgaa tctctcttgg gtcttacggt ctacgggagt aatgatcaag
      481 atccttacgt tactctgaaa gatacagaac aatatgaacg tgaagatttc ttgattaagc
      541 ccagtgataa tcttatagtt tgtggccgag ctgaacagga ccagtgcaat ttagaggtgc
      601 atgtttataa tcaagaagaa gactcttttt atgtacacca tgatatactc ttgtctgcat
      661 atcctctgag tgtggaatgg ctgaattttg atcctagccc agatgattct actggaaatt
      721 acattgctgt aggaaacatg acccctgtta ttgaagtgtg ggaccttgat atagtggact
      781 ctttagagcc agtcttcaca ctcggaagta aactttcaaa aaagaagaaa aagaaaggaa
      841 agaagagttc ctcagcagaa gggcataccg atgctgtcct tgacctttca tggaataagc
      901 taatcagaaa tgttttagca agtgcatcag ctgacaacac tgtaattctg tgggatatgt
      961 ccttggggaa accagcagct agcctcgctg tacacacaga caaggtccaa acactgcagt
     1021 ttcatccatt tgaagcacag actctgattt ctggctcata tgataagtca gtggctttgt
     1081 atgactgccg aagtccagat gaaagccatc gaatgtggcg attcagtggg cagatagaga
     1141 gagtgacttg gaatcacttt tcaccttgtc atttcttggc cagtacagat gacggctttg
     1201 tatataattt ggatgcacgt tcagataagc caatttttac acttaatgca cacaatgatg
     1261 aaatctctgg tcttgatctt agcagtcaaa tcaagggctg tctcgtgact gcttcagctg
     1321 acaaatacgt gaagatctgg gacatcttag gagataggcc aagtctagtt cattctaggg
     1381 acatgaaaat gggagttctc ttctgttctt catgttgccc tgatttgcca tttatttatg
     1441 cctttggagg tcaaaaagaa gggcttcggg tctgggatat aagcacagtc tcttcagtaa
     1501 atgaagcatt tggaagacga gagaggcttg ttcttgggag tgcaagaaat tcatctatta
     1561 gtggcccttt tggcagcagg agctcagata cacccatgga gtcttaatga agatcatcta
     1621 atttcctgct taccttaact gggaatttta aaaagttggc ctaaaaatgt tccatgcgtg
     1681 gcagcaacca tgcagagtga ctgaaacaca attcatttct gactgacatt cctttctgca
     1741 actgcggtgg caccacaaat atccggtctt tgtgcttgct cttcagatgg atggtttgta
     1801 aggctcttgt tgcatttctt aaaaaagagt aataaaaagg atttttaaaa agtaattcct
     1861 taaacatacc atctgtcaca gttaatctag atttgtaaat aggtagtaat ttatagaatt
     1921 tttaaagcgt aaaatccggt aatattaaaa gataggtaaa cctaggcctg gaaaggctgt
     1981 tatttggcta aaattgcaca ggaggccatg aacagaggca agtgccccag agactccact
     2041 ttcattccta actgttctca aattaatgct catgattgag tattctcagt gcaactcgta
     2101 gagtttgata agtaaaagtt acatgcccct gttttcctag catgatattc actgttatca
     2161 aagacaagag gcagaccatt cattcattct caaaacactg aatgccattc tgtgcctagt
     2221 gctatacaag gcatgggaga ttcagtgtga ataagtcttt gctctccacc taacaaggga
     2281 cagttttaat tatagattgt cttcctatta agtatgagtt ttagtaggca ttaaaaatcg
     2341 taattagttt gataatatga gacccaaccc taacttgcca gaagagtaat cagttcatga
     2401 accattgata tttcctgtat atttcatgaa tgtgacttca gtcattctag tgttaatact
     2461 gtggaatgtc attggtgtag caacgtgggt tcaccaaaac acctttttat acaaaagaca
     2521 gatgtgtgaa ttaaagagat taaaggata
//
