LOCUS       NM_001317962            2472 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens PWP1 homolog, endonuclein (PWP1), transcript variant
            2, mRNA.
ACCESSION   NM_001317962
VERSION     NM_001317962.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Liu,Y., Mattila,J., Ventela,S., Yadav,L., Zhang,W., Lamichane,N.,
            Sundstrom,J., Kauko,O., Grenman,R., Varjosalo,M., Westermarck,J.
            and Hietakangas,V.
  TITLE     PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar
            Regulation of Ribosomal Gene Expression
  JOURNAL   Dev Cell 43 (2), 240-252 (2017)
   PUBMED   29065309
  REMARK    GeneRIF: High PWP1 expression is associated with head and neck
            squamous cell carcinoma.
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Ludvigsen,M., Ostergaard,M., Vorum,H., Jacobsen,C. and Honore,B.
  TITLE     Identification and characterization of endonuclein binding
            proteins: evidence of modulatory effects on signal transduction and
            chaperone activity
  JOURNAL   BMC Biochem 10, 34 (2009)
   PUBMED   20028516
  REMARK    GeneRIF: Endonuclein interacts with several proteins indicating a
            broad function including signal transduction and chaperone activity
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2472)
  AUTHORS   Honore,B., Baandrup,U., Nielsen,S. and Vorum,H.
  TITLE     Endonuclein is a cell cycle regulated WD-repeat protein that is
            up-regulated in adenocarcinoma of the pancreas
  JOURNAL   Oncogene 21 (7), 1123-1129 (2002)
   PUBMED   11850830
REFERENCE   4  (bases 1 to 2472)
  AUTHORS   Honore,B., Leffers,H., Madsen,P. and Celis,J.E.
  TITLE     Cloning of a cDNA encoding a novel human nuclear phosphoprotein
            belonging to the WD-40 family
  JOURNAL   Gene 151 (1-2), 291-296 (1994)
   PUBMED   7828893
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK296252.1, AC007622.28 and
            CA449439.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001317962.1.
            
            Summary: The protein encoded by this gene contains several WD-40
            repeats and is found mostly in the nucleus. The expression and
            localization of this protein are cell cycle dependent. Expression
            of this gene is upregulated in pancreatic adenocarcinoma. Three
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (2) uses an alternate first exon
            in place of that of variant 1. The resulting isoform (2) is shorter
            at the N-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK296252.1, SRR14038193.1217956.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151119, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1722              AK296252.1         2-1723
            1723-2448           AC007622.28        79146-79871         c
            2449-2472           CA449439.1         16-39               c
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..2472
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="PWP1 homolog, endonuclein"
                     /db_xref="GeneID:11137"
                     /db_xref="HGNC:HGNC:17015"
                     /db_xref="MIM:620055"
     exon            1..96
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            97..155
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            156..343
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     CDS             211..1530
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="isoform 2 is encoded by transcript variant 2;
                     nuclear phosphoprotein similar to S. cerevisiae PWP1;
                     periodic tryptophan protein 1 homolog; keratinocyte
                     protein IEF SSP 9502; endonuclein"
                     /codon_start=1
                     /product="periodic tryptophan protein 1 homolog isoform 2"
                     /protein_id="NP_001304891.1"
                     /db_xref="CCDS:CCDS81735.1"
                     /db_xref="GeneID:11137"
                     /db_xref="HGNC:HGNC:17015"
                     /db_xref="MIM:620055"
                     /translation="MQSARTQARPREPLEDGDPEDDRTLDDDELAEYDLDKYDEEGDP
                     DAETLGESLLGLTVYGSNDQDPYVTLKDTEQYEREDFLIKPSDNLIVCGRAEQDQCNL
                     EVHVYNQEEDSFYVHHDILLSAYPLSVEWLNFDPSPDDSTGNYIAVGNMTPVIEVWDL
                     DIVDSLEPVFTLGSKLSKKKKKKGKKSSSAEGHTDAVLDLSWNKLIRNVLASASADNT
                     VILWDMSLGKPAASLAVHTDKVQTLQFHPFEAQTLISGSYDKSVALYDCRSPDESHRM
                     WRFSGQIERVTWNHFSPCHFLASTDDGFVYNLDARSDKPIFTLNAHNDEISGLDLSSQ
                     IKGCLVTASADKYVKIWDILGDRPSLVHSRDMKMGVLFCSSCCPDLPFIYAFGGQKEG
                     LRVWDISTVSSVNEAFGRRERLVLGSARNSSISGPFGSRSSDTPMES"
     exon            344..429
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            430..526
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            527..637
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            638..768
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            769..830
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            831..927
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            928..989
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1101
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1192
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1314
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1420
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..2472
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1754..1759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="hexamer: AATAAA"
     polyA_site      1778
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="major polyA site"
     regulatory      2453..2458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
                     /note="hexamer: ATTAAA"
     polyA_site      2472
                     /gene="PWP1"
                     /gene_synonym="IEF-SSP-9502; NCLB"
ORIGIN      
        1 aacgtggacc cggaactcgg ggcgttggct ggtgcgactg gctggggtga gggcggcttt
       61 gcccggattg tcgcgactag agctgcagtt cagaaggtag agctgagtaa agaagaagta
      121 aaacgcctca ttgctgaggc aaaggagaaa ttgcaagaag aaggtggtgg cagtgatgaa
      181 gaggagacag gcagtccttc agaagatggc atgcagagtg cacgcaccca ggcacgccca
      241 agagagcccc tggaggatgg tgacccagag gatgacagga cgcttgatga tgatgagctg
      301 gctgagtacg acttagataa atatgatgag gaaggtgacc cagatgctga gactcttggt
      361 gaatctctct tgggtcttac ggtctacggg agtaatgatc aagatcctta cgttactctg
      421 aaagatacag aacaatatga acgtgaagat ttcttgatta agcccagtga taatcttata
      481 gtttgtggcc gagctgaaca ggaccagtgc aatttagagg tgcatgttta taatcaagaa
      541 gaagactctt tttatgtaca ccatgatata ctcttgtctg catatcctct gagtgtggaa
      601 tggctgaatt ttgatcctag cccagatgat tctactggaa attacattgc tgtaggaaac
      661 atgacccctg ttattgaagt gtgggacctt gatatagtgg actctttaga gccagtcttc
      721 acactcggaa gtaaactttc aaaaaagaag aaaaagaaag gaaagaagag ttcctcagca
      781 gaagggcata ccgatgctgt ccttgacctt tcatggaata agctaatcag aaatgtttta
      841 gcaagtgcat cagctgacaa cactgtaatt ctgtgggata tgtccttggg gaaaccagca
      901 gctagcctcg ctgtacacac agacaaggtc caaacactgc agtttcatcc atttgaagca
      961 cagactctga tttctggctc atatgataag tcagtggctt tgtatgactg ccgaagtcca
     1021 gatgaaagcc atcgaatgtg gcgattcagt gggcagatag agagagtgac ttggaatcac
     1081 ttttcacctt gtcatttctt ggccagtaca gatgacggct ttgtatataa tttggatgca
     1141 cgttcagata agccaatttt tacacttaat gcacacaatg atgaaatctc tggtcttgat
     1201 cttagcagtc aaatcaaggg ctgtctcgtg actgcttcag ctgacaaata cgtgaagatc
     1261 tgggacatct taggagatag gccaagtcta gttcattcta gggacatgaa aatgggagtt
     1321 ctcttctgtt cttcatgttg ccctgatttg ccatttattt atgcctttgg aggtcaaaaa
     1381 gaagggcttc gggtctggga tataagcaca gtctcttcag taaatgaagc atttggaaga
     1441 cgagagaggc ttgttcttgg gagtgcaaga aattcatcta ttagtggccc ttttggcagc
     1501 aggagctcag atacacccat ggagtcttaa tgaagatcat ctaatttcct gcttacctta
     1561 actgggaatt ttaaaaagtt ggcctaaaaa tgttccatgc gtggcagcaa ccatgcagag
     1621 tgactgaaac acaattcatt tctgactgac attcctttct gcaactgcgg tggcaccaca
     1681 aatatccggt ctttgtgctt gctcttcaga tggatggttt gtaaggctct tgttgcattt
     1741 cttaaaaaag agtaataaaa aggattttta aaaagtaatt ccttaaacat accatctgtc
     1801 acagttaatc tagatttgta aataggtagt aatttataga atttttaaag cgtaaaatcc
     1861 ggtaatatta aaagataggt aaacctaggc ctggaaaggc tgttatttgg ctaaaattgc
     1921 acaggaggcc atgaacagag gcaagtgccc cagagactcc actttcattc ctaactgttc
     1981 tcaaattaat gctcatgatt gagtattctc agtgcaactc gtagagtttg ataagtaaaa
     2041 gttacatgcc cctgttttcc tagcatgata ttcactgtta tcaaagacaa gaggcagacc
     2101 attcattcat tctcaaaaca ctgaatgcca ttctgtgcct agtgctatac aaggcatggg
     2161 agattcagtg tgaataagtc tttgctctcc acctaacaag ggacagtttt aattatagat
     2221 tgtcttccta ttaagtatga gttttagtag gcattaaaaa tcgtaattag tttgataata
     2281 tgagacccaa ccctaacttg ccagaagagt aatcagttca tgaaccattg atatttcctg
     2341 tatatttcat gaatgtgact tcagtcattc tagtgttaat actgtggaat gtcattggtg
     2401 tagcaacgtg ggttcaccaa aacacctttt tatacaaaag acagatgtgt gaattaaaga
     2461 gattaaagga ta
//
