LOCUS       NM_144709               4025 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant
            1, mRNA.
ACCESSION   NM_144709
VERSION     NM_144709.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Mukhopadhyay,S., Deogharia,M. and Gupta,R.
  TITLE     Mammalian nuclear TRUB1, mitochondrial TRUB2, and cytoplasmic PUS10
            produce conserved pseudouridine 55 in different sets of tRNA
  JOURNAL   RNA 27 (1), 66-79 (2021)
   PUBMED   33023933
  REMARK    GeneRIF: Mammalian nuclear TRUB1, mitochondrial TRUB2, and
            cytoplasmic PUS10 produce conserved pseudouridine 55 in different
            sets of tRNA.
REFERENCE   3  (bases 1 to 4025)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4025)
  AUTHORS   Song,J., Zhuang,Y., Zhu,C., Meng,H., Lu,B., Xie,B., Peng,J., Li,M.
            and Yi,C.
  TITLE     Differential roles of human PUS10 in miRNA processing and tRNA
            pseudouridylation
  JOURNAL   Nat Chem Biol 16 (2), 160-169 (2020)
   PUBMED   31819270
  REMARK    GeneRIF: PUS10 differentially participates in the metabolic
            pathways of miRNA and tRNA. In the nucleus, PUS10 promotes miRNA
            biogenesis via interaction with pri-miRNA.In the cytoplasm, PUS10
            modifies tRNA, and its pseudouridylation activity is required for
            cell growth.
REFERENCE   5  (bases 1 to 4025)
  AUTHORS   Deogharia,M., Mukhopadhyay,S., Joardar,A. and Gupta,R.
  TITLE     The human ortholog of archaeal Pus10 produces pseudouridine 54 in
            select tRNAs where its recognition sequence contains a modified
            residue
  JOURNAL   RNA 25 (3), 336-351 (2019)
   PUBMED   30530625
REFERENCE   6  (bases 1 to 4025)
  AUTHORS   Park,S.Y., Shin,J.N., Woo,H.N., Piya,S., Moon,A.R., Seo,Y.W.,
            Seol,D.W. and Kim,T.H.
  TITLE     DOBI is cleaved by caspases during TRAIL-induced apoptotic cell
            death
  JOURNAL   BMB Rep 42 (8), 511-515 (2009)
   PUBMED   19712588
  REMARK    GeneRIF: These data suggest that the cleaved DOBI may acquire a new
            function, possibly by cooperating with tBid in the mitochondrial
            event of cell death caused by TRAIL.
REFERENCE   7  (bases 1 to 4025)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 4025)
  AUTHORS   Anderson,C.A., Massey,D.C., Barrett,J.C., Prescott,N.J.,
            Tremelling,M., Fisher,S.A., Gwilliam,R., Jacob,J., Nimmo,E.R.,
            Drummond,H., Lees,C.W., Onnie,C.M., Hanson,C., Blaszczyk,K.,
            Ravindrarajah,R., Hunt,S., Varma,D., Hammond,N., Lewis,G.,
            Attlesey,H., Watkins,N., Ouwehand,W., Strachan,D., McArdle,W.,
            Lewis,C.M., Lobo,A., Sanderson,J., Jewell,D.P., Deloukas,P.,
            Mansfield,J.C., Mathew,C.G., Satsangi,J. and Parkes,M.
  CONSRTM   Wellcome Trust Case Control Consortium
  TITLE     Investigation of Crohn's disease risk loci in ulcerative colitis
            further defines their molecular relationship
  JOURNAL   Gastroenterology 136 (2), 523-9 (2009)
   PUBMED   19068216
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 4025)
  AUTHORS   McCleverty,C.J., Hornsby,M., Spraggon,G. and Kreusch,A.
  TITLE     Crystal structure of human Pus10, a novel pseudouridine synthase
  JOURNAL   J Mol Biol 373 (5), 1243-1254 (2007)
   PUBMED   17900615
  REMARK    GeneRIF: crystal structure of Pus10 (previously denoted as
            FLJ32312) at 2.0 A resolution and modeling studies describe protein
            domains and active sites
REFERENCE   10 (bases 1 to 4025)
  AUTHORS   Aza-Blanc,P., Cooper,C.L., Wagner,K., Batalov,S., Deveraux,Q.L. and
            Cooke,M.P.
  TITLE     Identification of modulators of TRAIL-induced apoptosis via
            RNAi-based phenotypic screening
  JOURNAL   Mol Cell 12 (3), 627-637 (2003)
   PUBMED   14527409
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL832208.1, BP354604.1, BC101680.1, AC010733.8 and AA878516.1.
            
            On Nov 23, 2018 this sequence version replaced NM_144709.3.
            
            Summary: Pseudouridination, the isomerization of uridine to
            pseudouridine, is the most common posttranscriptional nucleotide
            modification found in RNA and is essential for biologic functions
            such as spliceosome biogenesis. Pseudouridylate synthases, such as
            PUS10, catalyze pseudouridination of structural RNAs, including
            transfer, ribosomal, and splicing RNAs. These enzymes also act as
            RNA chaperones, facilitating the correct folding and assembly of
            tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by
            OMIM, May 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.82250.1, AK056874.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316752.11/ ENSP00000326003.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AL832208.1         6-141
            137-204             BP354604.1         121-188
            205-1930            BC101680.1         1-1726
            1931-3622           AC010733.8         94081-95772         c
            3623-3835           AA878516.1         11-223              c
            3836-4025           AC010733.8         93678-93867         c
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.1-p15"
     gene            1..4025
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="pseudouridine synthase 10"
                     /db_xref="GeneID:150962"
                     /db_xref="HGNC:HGNC:26505"
                     /db_xref="MIM:612787"
     exon            1..248
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            249..389
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     CDS             264..1853
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /EC_number="5.4.99.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     coiled-coil domain containing 139; putative tRNA
                     pseudouridine synthase Pus10; psi55 synthase; tRNA-uridine
                     isomerase; tRNA pseudouridylate synthase; tRNA
                     pseudouridine 55 synthase; pseudouridylate synthase 10;
                     coiled-coil domain-containing protein 139"
                     /codon_start=1
                     /product="tRNA pseudouridine synthase Pus10 isoform 1"
                     /protein_id="NP_653310.2"
                     /db_xref="CCDS:CCDS1865.1"
                     /db_xref="GeneID:150962"
                     /db_xref="HGNC:HGNC:26505"
                     /db_xref="MIM:612787"
                     /translation="MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKEL
                     LNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASK
                     NSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAW
                     LLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPE
                     TVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVC
                     AVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAES
                     FNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDL
                     QLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLH
                     RRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVT
                     ADILELDVESVDVDWPPALDD"
     exon            390..644
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            645..731
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            732..766
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            767..878
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            879..940
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            941..986
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1051
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1137
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1263
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1320
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..1397
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1453
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1571
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1714
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1814
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..4025
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2262..2267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="hexamer: AATAAA"
     polyA_site      2280
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="major polyA site"
     regulatory      3586..3591
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="hexamer: AATGAA"
     polyA_site      3613
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
     regulatory      3815..3820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="hexamer: CATAAA"
     polyA_site      3835
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="major polyA site"
     regulatory      4003..4008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
                     /note="hexamer: AATAAA"
     polyA_site      4025
                     /gene="PUS10"
                     /gene_synonym="CCDC139; DOBI; Hup10"
ORIGIN      
        1 agagactttg ctccctgtcg atgccgtggc tggacaaatg ccgctctcaa ctgctacaga
       61 ccaaagtcta cgctttaaag atagagaagc cattctgtca aagtgaaaac agaagcggta
      121 ggtaggaagc cgcccccctt tctttcaagc acagagaccc agagagacgc ccaggagctc
      181 ccgaaaaaaa cgttgctgat tagagctgcc gggtcagaaa cagctgtgag acactaaagt
      241 ccccactgtt ataattattc aatatgttcc cactgactga ggaaaacaag catgtggccc
      301 agttgttgct caatactggt acttgtccaa gatgtatctt cagattctgt ggtgtggatt
      361 ttcatgcacc ttacaaactt ccatacaagg agttgctcaa tgaactacag aaatttctgg
      421 aaactgaaaa agatgaatta attttggaag ttatgaaccc acctcccaag aaaattcgac
      481 tgcaagaact ggaagatagt attgataatc taagtcaaaa tggagaggga aggatctctg
      541 ttagtcatgt tggaagcact gcttccaaga actcaaattt aaatgtatgt aatgtatgcc
      601 taggaattct tcaagaattc tgtgagaaag atttcattaa aaaggtgtgc caaaaggttg
      661 aggcctctgg gtttgaattc accagcttgg tattttcagt ctccttccca ccacaactat
      721 ctgtaagaga gcatgctgca tggttgctgg taaaacagga aatgggaaag cagagtctgt
      781 cgctgggaag agatgatata gttcagctaa aagaagccta caaatggata actcaccccc
      841 tgttttcaga ggaactgggt gttcccattg atggaaagag cttgtttgaa gtgagtgtgg
      901 tctttgctca cccagaaaca gttgaggatt gccacttcct agctgcgata tgcccagatt
      961 gttttaagcc agccaaaaac aaacagtctg tattcactag aatggcagtt atgaaagcct
     1021 tgaataagat aaaggaagag gatttcctta agcagtttcc ttgtcctcca aactcaccaa
     1081 aggctgtatg cgctgttctt gaaattgaat gtgctcatgg tgctgttttt gtggctggga
     1141 gatataataa atactccagg aatctaccac aaactccttg gataattgat ggagaaagga
     1201 agctggaatc ttcagtggaa gaattaattt cagatcatct gttggcagta tttaaagcag
     1261 agagttttaa tttttcatcc tctggaagag aagatgtaga tgtgagaaca ttaggaaatg
     1321 gaaggccctt tgcaattgag ctggtgaatc ctcatagagt acatttcact tcacaagaaa
     1381 ttaaggaact tcagcagaaa attaataact catctaacaa aatccaagta cgtgacttgc
     1441 agcttgtcac aagagaggca ataggacata tgaaagaagg tgaagaagaa aagacaaaga
     1501 cctacagtgc cttaatttgg acaaataaag cgatacagaa gaaagacatt gaattcctaa
     1561 atgacataaa ggacttaaaa atcgaccaga aaacaccttt gcgcgtcctt caccgaaggc
     1621 ccctggctgt gcgagctcgc gtcattcact tcatggagac acagtacgtg gatgagcacc
     1681 acttccgcct ccacttgaaa actcaggctg gcacctacat taaagagttt gtacatggag
     1741 acttcgggag aaccaagcca aacattgggt ccctgatgaa tgtgactgca gacattctgg
     1801 agctggatgt tgagtctgta gatgttgact ggccacctgc tctggatgac tagctttcaa
     1861 atttggagac aaagagtatg gttttcctgg catgatgtgg acatccatgg agcacatgcc
     1921 gtaaaatggc tgtttaccca ccataacggt gtcttgaaaa ctatttggat catgttgatc
     1981 tatataattg ttaatttgtt gtaacatctc aggatctata tatgtgtata ttttgtgtta
     2041 aattgttcca aggatgtctt aggatttttc tcattccctc tttcaccccc acaaaccaaa
     2101 ctatgaataa tgaaataatt ctccttaatt ctttcattta gagaggtgca caaacaggac
     2161 acattctctg ttaacctaag aagctgtaat ttcagcaaga tttccctcca caagagatat
     2221 accaccttta aaatcatgtt ctaatttttg taaattatct gaataaaagt tatatctaga
     2281 tttatgcagc ataatatcct ataatagtct aagattgcaa atgcaaatct gacatcatta
     2341 ttttccccag gaagcctcaa acaagctatt accaatgaag ttcttttggc tgtgatttgt
     2401 cataaatctt aaccaaacta tgaataatga aataattctc attaatactt tcattaagaa
     2461 agaattttga caatctaaaa ttgtcaatat aggtttcctg tggagggaaa gtaaaaccgt
     2521 aggagccaag gcatgccatg gtaggagcct ttcataaatc ttttaaataa ggagaaacaa
     2581 gtgcctttaa taacttcaat cccagggtta agaatttgaa aaattgagat tgctaaacaa
     2641 agacgaactg gtattatttc agaatgcttc aatatattat ccaattataa acattttaat
     2701 aataccttat taaagtagat aggatattgt ctccatttta taaatatgga aaccaaagac
     2761 tagaagttaa atgacttatc caaaattgtt agctacttct atattgagga ctagaactga
     2821 agtttcttgg tttctagggt ctttctaata agccctatta ctgagcacct gtttgtgtga
     2881 aggggcagct atggatgcca tttaaactca tgtaactgat aattaacttt tggaatgtag
     2941 ttaccaaatc aaagagctta ccaaaaaata gcccaagact atttaataca atcttatata
     3001 gttaccattt gttattaagt taaacctcag ttggtattat gaagaggaaa aacattaagc
     3061 taaacctaaa aaaaatgaat gggtcattta agtttcagaa aggagcctca ttacaatttc
     3121 agatttgagt ataatttcca tcaccctaat tcaactattt gagctttttt taagaataca
     3181 tgtgacttga ctagtattag attactaacc atttcccatc tctatattaa acataaatca
     3241 cagataccaa gattcacttt agatatgagg aaaatggttt taatggacac aaaggagtca
     3301 gccacgttgg aaccaacata gtttcatacc acgttgaaac catgtgtttg atatgcaaat
     3361 aacagcaaat aattttttca ctcttgtcaa tgccaatgca ttgaaaggcc cagaaaatga
     3421 gaaaaggata acaaactttt gataaaaagg taagaatttc tgtgtggatg ttcctcagtt
     3481 tctgggaaaa ttttgctaca aaagagattt aaataattta ggaggcagtc ttttgcatgg
     3541 tatttttggc ttacctttga atctagtgga cctgtaatat gtaagaatga agaatgtgct
     3601 tagcaaactg aaatgagaag atttctattt cttaaatttc tattcctgag attatataaa
     3661 atttcacgca aagagtaagc attagcttta aatacttctt ccaaatgttc ctagaatttt
     3721 gagacaaaga ttgttaaaaa ttaatgtatg tctttgtttt aggcagaact ggaaatatta
     3781 agtagtgaga gtactatagt ttcccttttg cctacataaa agcattttaa aattagtttt
     3841 atgatttctt tgtaagcttt atttaaattc aaagtctttt tcataacact taagagaaag
     3901 agccctttgt acttcttagt atctcttata tagtcccaaa tatagtctgt ggtttatttt
     3961 aggaaaaaca tcaactctga atacctggaa aagtcaaaag aaaataaagt tggtaaaatg
     4021 aaaaa
//
