LOCUS       NM_033224               9232 bp    mRNA    linear   PRI 14-APR-2024
DEFINITION  Homo sapiens purine rich element binding protein B (PURB), mRNA.
ACCESSION   NM_033224
VERSION     NM_033224.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Kelm,R.J. Jr., Lamba,G.S., Levis,J.E. and Holmes,C.E.
  TITLE     Characterization of purine-rich element binding protein B as a
            novel biomarker in acute myelogenous leukemia prognostication
  JOURNAL   J Cell Biochem 119 (2), 2073-2083 (2018)
   PUBMED   28834593
  REMARK    GeneRIF: Results showed that Purbeta ssDNA-binding activity was
            significantly increased in myeloid cells from AML patients
            suggesting that the enhanced ssDNA-binding activity of Purbeta in
            transformed myeloid cells may serve as a unique and measurable
            phenotypic trait for improving prognostic risk stratification in
            AML.
REFERENCE   3  (bases 1 to 9232)
  AUTHORS   Rumora,A.E., Ferris,L.A., Wheeler,T.R. and Kelm,R.J. Jr.
  TITLE     Electrostatic and Hydrophobic Interactions Mediate Single-Stranded
            DNA Recognition and Acta2 Repression by Purine-Rich Element-Binding
            Protein B
  JOURNAL   Biochemistry 55 (19), 2794-2805 (2016)
   PUBMED   27064749
  REMARK    GeneRIF: site-directed mutagenesis revealed several basic amino
            acid residues in the intermolecular (R267) and intramolecular (K82
            and R159) subdomains that are essential for Purbeta transcriptional
            repressor function in Acta2 promoter-reporter assays. In keeping
            with their diminished Acta2 repressor activity in fibroblasts,
            purified Purbeta variants containing an R267A mutation exhibited
            reduced binding affinity for purine-ric...
REFERENCE   4  (bases 1 to 9232)
  AUTHORS   Hariharan,S., Kelm,R.J. Jr. and Strauch,A.R.
  TITLE     The Puralpha/Purbeta single-strand DNA-binding proteins attenuate
            smooth-muscle actin gene transactivation in myofibroblasts
  JOURNAL   J Cell Physiol 229 (9), 1256-1271 (2014)
   PUBMED   24446247
  REMARK    GeneRIF: Suggest dynamic interplay between transcriptional
            activators Pur-alpha/Pur-beta and repressors in regulating SMalphaA
            gene output during myofibroblast differentiation.
REFERENCE   5  (bases 1 to 9232)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 9232)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   7  (bases 1 to 9232)
  AUTHORS   Gupta,M., Sueblinvong,V., Raman,J., Jeevanandam,V. and Gupta,M.P.
  TITLE     Single-stranded DNA-binding proteins PURalpha and PURbeta bind to a
            purine-rich negative regulatory element of the alpha-myosin heavy
            chain gene and control transcriptional and translational regulation
            of the gene expression. Implications in the repression of
            alpha-myosin heavy chain during heart failure
  JOURNAL   J Biol Chem 278 (45), 44935-44948 (2003)
   PUBMED   12933792
  REMARK    GeneRIF: results show that PUR proteins are capable of binding to
            alpha-MHC mRNA and attenuate its translational efficiency; also
            show robust expression of PUR proteins in failing hearts where
            alpha-MHC mRNA levels are suppressed
REFERENCE   8  (bases 1 to 9232)
  AUTHORS   Lezon-Geyda,K., Najfeld,V. and Johnson,E.M.
  TITLE     Deletions of PURA, at 5q31, and PURB, at 7p13, in myelodysplastic
            syndrome and progression to acute myelogenous leukemia
  JOURNAL   Leukemia 15 (6), 954-962 (2001)
   PUBMED   11417483
REFERENCE   9  (bases 1 to 9232)
  AUTHORS   Kelm,R.J. Jr., Cogan,J.G., Elder,P.K., Strauch,A.R. and Getz,M.J.
  TITLE     Molecular interactions between single-stranded DNA-binding proteins
            associated with an essential MCAT element in the mouse smooth
            muscle alpha-actin promoter
  JOURNAL   J Biol Chem 274 (20), 14238-14245 (1999)
   PUBMED   10318844
REFERENCE   10 (bases 1 to 9232)
  AUTHORS   Bergemann,A.D., Ma,Z.W. and Johnson,E.M.
  TITLE     Sequence of cDNA comprising the human pur gene and
            sequence-specific single-stranded-DNA-binding properties of the
            encoded protein
  JOURNAL   Mol Cell Biol 12 (12), 5673-5682 (1992)
   PUBMED   1448097
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004854.3.
            
            On May 17, 2019 this sequence version replaced NM_033224.4.
            
            Summary: This gene product is a sequence-specific, single-stranded
            DNA-binding protein. It binds preferentially to the single strand
            of the purine-rich element termed PUR, which is present at origins
            of replication and in gene flanking regions in a variety of
            eukaryotes from yeasts through humans. Thus, it is implicated in
            the control of both DNA replication and transcription. Deletion of
            this gene has been associated with myelodysplastic syndrome and
            acute myelogenous leukemia. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR1803611.133393.1, SRR1803615.68634.1
                                        [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395699.5/ ENSP00000379051.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9232              AC004854.3         35146-44377
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p13"
     gene            1..9232
                     /gene="PURB"
                     /gene_synonym="PURBETA"
                     /note="purine rich element binding protein B"
                     /db_xref="GeneID:5814"
                     /db_xref="HGNC:HGNC:9702"
                     /db_xref="MIM:608887"
     exon            1..9232
                     /gene="PURB"
                     /gene_synonym="PURBETA"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..1121
                     /gene="PURB"
                     /gene_synonym="PURBETA"
                     /note="transcriptional activator protein Pur-beta"
                     /codon_start=1
                     /product="transcriptional regulator protein Pur-beta"
                     /protein_id="NP_150093.1"
                     /db_xref="CCDS:CCDS5499.1"
                     /db_xref="GeneID:5814"
                     /db_xref="HGNC:HGNC:9702"
                     /db_xref="MIM:608887"
                     /translation="MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNK
                     RFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSP
                     EQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAG
                     PGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGE
                     LPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYA
                     DEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED"
     regulatory      9210..9215
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PURB"
                     /gene_synonym="PURBETA"
                     /note="hexamer: AATAAA"
     polyA_site      9232
                     /gene="PURB"
                     /gene_synonym="PURBETA"
                     /note="major polyA site"
ORIGIN      
        1 agggctgcac ggaagtcgcg ggggcggggc gcggaggcga tgggggggcg gcggcggccg
       61 gcgatgagcg gcgggagcgc gtgagcgagc cgggcgggcg aggctggggg gccccggagc
      121 gcgagccgga gggcgggggc gcgagcgcgg cgggcggtgg cggcgaggcg gcggcctaga
      181 agatggcgga cggcgacagc ggcagcgagc gcggcggcgg cggtgggccg tgcgggttcc
      241 agcccgcgtc ccgcggcggc ggcgagcaag agacgcagga gctggcctcg aagcggctgg
      301 acatccagaa caagcgcttc tacttagatg tgaagcagaa cgccaagggc cgcttcctca
      361 agatcgccga ggtgggcgcg ggcggttcca agagccgcct cacgctgtcc atggcggtgg
      421 ccgccgagtt ccgcgactcg ctgggcgact tcatagaaca ctacgcgcag ctgggcccta
      481 gcagccccga gcagctggcg gctggcgccg aggagggcgg cgggccgcgg cgcgcgctca
      541 agagcgaatt cttggtgcgt gagaaccgca agtactacct ggacctcaag gagaaccagc
      601 gcggccgctt cctgcgcatc cgccaaacgg tcaaccgcgg cggtggcggc ttcggcgcgg
      661 gccccgggcc gggcggcttg cagagcggcc agaccatcgc gctgcctgcg cagggcctca
      721 tcgagttccg cgacgcgctg gcgaagctca tagacgacta cggaggcgag gacgacgagc
      781 tggcaggcgg cccgggaggc ggcgccgggg gcccaggggg cggcctgtat ggagagctcc
      841 cggagggcac ctccatcacc gtggactcca agcgcttctt cttcgatgtg ggctgcaaca
      901 aatacggggt gtttctgcga gtgagcgagg tgaagccgtc ctaccgcaat gccatcaccg
      961 tacccttcaa agcctggggc aagttcggag gcgccttttg ccggtatgcg gatgagatga
     1021 aagaaatcca ggaacgacag agggataagc tttatgagcg acgtggtggg ggcagcggcg
     1081 gcggcgaaga gtcagagggt gaggaggtgg atgaggattg aaacgggcag cttcccctac
     1141 aggcctccac ccaaccacca tccccttggc tagagaattc cctttcctgc tcatccccag
     1201 tgagctagtg gagagggagc aagggaggcc gcagagggaa aaaacaaaac gtaacacagt
     1261 taagagaaat aatcgtaaga gaacagtgac ggacaacttg agaaaagcag tcaagttcca
     1321 aggaactgac agcaacctgc aaagaggaaa acagcatctc ctcacctgcg taaaattgtc
     1381 tcagcttctg ttgtttctca actgaggttc gtaaacccat caggataatc cctggaggga
     1441 atagatcctt gcacatccag ggcaagaaac atgtccaagt tacccagacc attgataaca
     1501 gttgcattta ggttgcacct gggtaatctg gcataaaaga tctctctagg cctcactgtt
     1561 gcggtgtcta tcccttcacc tccattgaaa tcagcatttt ggatctaggt cttcatggaa
     1621 tccttgagaa gagaggcctt tacaattacc cagttctgag ggttcaggtt cacgaaaaga
     1681 aatgcaactt gggataatca tgaacaggtt aaagataaga tttcaagaag ccatctaaga
     1741 atacagaacc aaattggatc cattttttta aaaaaatggt tttgcatgga acctggacca
     1801 aggcaaatgt cttttcttcg cagaattgtt ttccaggatg ccagtggatt cagatagcaa
     1861 tgcttggagt agaatccgtt actaaaatag tttcaaagtt gacaaaaaat tttcaaagat
     1921 aaaagcagtt ttacattggg ggttgctgag gtaggcacaa gaaaaagtca ggcataaagc
     1981 acaaggcaga ctgtttgagt ggattggttg ctgctcacta aagttgttcc cctgatctct
     2041 aaatatggag gtcattacca agaaatgctt tggtatgaat gagagccaga tctccactgt
     2101 gtgagccagt gaattatggc taattcggct gttacagcca ctggttggct ggattttaaa
     2161 ccataaaact tgaagattac ctacaaaagt aacagtgtgg ctataagcct gagctttaat
     2221 ggatatacat cctcacagaa aagttggaaa taaccaaaac tgaagtctta atttaccttc
     2281 agtttaatct gtggatttgt tcaaatacta aagatcctca ggtccagaat tccagcatca
     2341 tttattcttt taaaattttt aagaacttga tccattgtat cagtacctca caatcagagt
     2401 tggcaaatga tggatgagtg attcaagcag tgcacccggt ggaagctgaa atccatctgt
     2461 gaatggaact gaagtgaacg tgaatatgct gactatatcc tggaagcatt tttataccat
     2521 cttgaaattt caacaaactg gcttttgcca gttaatccag ctgtctttca agaataaaag
     2581 ttggggtttt caaggatcgc ctcttctata ttttaaatgg attttcagta gaaatgattt
     2641 ttactaatca agttaatccc accccatcaa aaggtattcc tagaaatgtc atagacctag
     2701 gtaactttga attgaatggg agctaacgtt ctttccaaag ttttcaggta ttctttgtgt
     2761 gacaccttct caaccaggag gcaagtaacc ccgcctccac aatcttagta ttttttttaa
     2821 actgcatgcc tgccccttat ttgagctgcc tttttaattt attgcatatc ctttttatta
     2881 tcttattttg gtattattca atctatacaa tctttttgta tttattggga aatgagtaat
     2941 atacaaaaag gttttcatgt atttgtggct gagagggcgg gaaataattg tgtacataaa
     3001 attaggcttt tttaaaaaaa tagattatga tgcagaatat tgttgatctt agattaaaaa
     3061 gtggaagagc cacaaacatt ggtgcccttt tcagactatt tctctactct catcatccac
     3121 agtagaattt ttaaacagat ttttttaaag cttttctttt aaatttttct ccgttgcaaa
     3181 gaatgtttcc taaattgtat gggagcaata gtatttttga tgttttaatg acatccgtat
     3241 acttgtactg tattttgtac tacaaggcag ctgtttttca ataatgtcct gctgtattta
     3301 cctacgtgtt ttgagtgtct atttctttgc tgcggagaac aaattcctaa atagttttag
     3361 taaaggagct gagaagctag cattaggttt gcagaaacta tttaagtttc aactctgagg
     3421 cagcaatgaa aatttaagtt gcagctatta gttgattgct gtaacttttt cattttcaaa
     3481 ccatgtacaa ttcttgtata gaccaacttg ttttcttgct tcagtggtgg ttctgttgct
     3541 cagctgcagt gagccagttc aattttgcaa aggtgcagta cctctccttt ttaaggggtt
     3601 ggtttattct tttttctttt tgtttggctg aattgcagta actagccttg cctttctatt
     3661 ctgtagaaat gacagggtct tcacaatcct tcaccagtgg ctactaagct ataattagct
     3721 gaatagaaag aatgtggaag tggtctgagg catatagagt atatgccaag aacactacca
     3781 tatatggcat cagctttggt taccagagaa attttcttag tcattagacc atgtaacagt
     3841 aatatatcat atgtaaatct ttagatatca atttgaaaat cctccaaaaa aaggagcaaa
     3901 gaatgcataa gctatgtgtt ggcaaaagta atttatatta aaattttgac ctgcctatgt
     3961 aagattaagt ggtaatgtca tagtggtggg tttttaagtc ttaaccaatc tctaaggttt
     4021 gtttctcctg caagggatgg ttcatggcct ctcttcccac tgcaggaaga ttgcagaagg
     4081 ctgtggatta attgtagcat ttcactgatc ttcactccag tcactaggga caatagaaac
     4141 ctgcaaaaca cagattcatt cgtaaatatt attaatagct tattaaagga aatggtcttt
     4201 gttaattcca gtcagataat ggcattgtac agacatggga aacaaccaat ttttttgttt
     4261 ttcagttgtt gctatgaatg attttgagcc ttttttttta agcttggcaa acatcccagc
     4321 taatcaaaat agtcatattc ctgagaagta ggaaactaaa acttcttttc atataattgt
     4381 tagaaggttt gtttcccaaa ctaccatagt tacaaaggtg aaaagccaaa ttttaggaac
     4441 agaatcaaaa gaataaaaat ctgtgaagag atcctactac tcttccttct atgttttggt
     4501 tttggtttct attgtcccta tcatttcagc aagtggaaca gcagcaagtt tttcagtgca
     4561 tatgctgcac aagaacaaaa tataaatctg tatggcacca aaaatcaaag tgaaaaccaa
     4621 accaaaaacc caaacaccct atgtaactat cggaggcata tacgtggtat aaatgactgt
     4681 agctgtgata cacacatggc tacttgtcac atcactttcc ataattattt actgcaaaat
     4741 gattgagagg cttttggtgc aggcagccgt taacctcctg cttcctttgt tacctctgga
     4801 ttactttgca gtaaattgca ggtcttttaa gagatttaag cttcagtttt ctcaaaacaa
     4861 aacaattatc ctgtcttatc tgaagatgca gggttgtggg caaaagaggc tggttataat
     4921 aatgccctca tattgagtgg tctgtaaacg gctgcacact tcaggcactg tagttgctga
     4981 agatgctttg ttaaatgtga ccttgactgg ctttacaggg gtgtagaatg taatctacac
     5041 aaggtgactt tgcatctatc ttgctcttga ggtggatgaa attgagaagc tggagtgtgt
     5101 aagccatgca cataagtatt cttcactgta aattttgttt tcatttttaa cccaattatg
     5161 gtactttatc caatgcacaa ctgatctctc agtagatatt catttgaaaa tagtgtggcc
     5221 ttgatcagtg agaaagggaa ggagaaaagt gacttttttg cttatgtaga aatgactcat
     5281 ttgctgagag tttgtctttc tgcagcactc ttggtatata gtgatcggtc tcctttttga
     5341 ttggggaaag ttaatgtttt tgaccctgga gttaattcag ttgagttatc ttatattttt
     5401 aggaagtatc agaattgctc tgatgaataa caaagttgac tgttttgatg tccaatctca
     5461 ggttttagaa tatagtggtg taaagtccca ctatttttaa ttcttaaaac aactttaatt
     5521 tcgtacaccc taaaagtcac atgcataagg cctgttcaga gagcagagcc tccatctttt
     5581 tgctcctttt ctactttgta cttcacttga aaaaatatca agtgacttta cattgtatat
     5641 ttccattgta accctgacat ttctcaaaga taaagcactt tttgatcatg aaatacatga
     5701 aatctttgtg tgatgtggat catagtttct caggctccct tagataattg cttatgaata
     5761 ttgttctaac tctgtgtaag aagagtagaa atctttgcta atgttagaag gtttgtatta
     5821 ttgatccaga atgcattttg ctagtttcca atggatggga gagtaaataa tgctgcattc
     5881 acaatttaat aagttacttt cccttgagcc ttaaggtaac tttttctttc ctgtcaacta
     5941 cagcactgaa gttatagtaa gtgaatgaga ttatcagttt tcagggttgg ttttagagta
     6001 ctgtaaatca attagctgtc ttcctaaaga gttacaactc ccattcagta tactggataa
     6061 tgggtgtgtg ggtggggctg gggagggcgg gagatagttt gtagaaaaga aaaaagaaaa
     6121 aaaaaaaaac acatttacct taagaaaata cagacaaaaa agaataaagt atgtgtcata
     6181 tgctccattt gagattccaa atgcggccgg gcgtggtggc tcacgcctgt aatcccagca
     6241 ctctgggagg ctgaggtggg cgggtcagtt caggtcagcc tggacaacat ggtgaaaccc
     6301 cgtctctact aaaaatacta aaattagccg ggcatggtgg tgcatgcctg tagtcccagc
     6361 tacttgggag gctgaggcag gagaatggca tgaacccagg aagcggaggt tgcagtgagc
     6421 caagatcgcg ccattgcact ccagcctggg taacagaaca agactccgtc tcaaaaaata
     6481 aataaataaa ttccaaatgt aaggtggaaa tggcagatta taacttcatt tcccccattt
     6541 ttaacttaga aaaaacaaag tccttaattt tcaagtatta gccccccaga agtttcagca
     6601 gatagctgga ctcttgaata atgatttctt ttgatatgtc tgactttgct atttaaagtc
     6661 tcctttccca gccctcagtc ttaaaactca gttttattac tgacggattt aacagactct
     6721 tacttgaaaa ttgtccaatt atcagtactg aaaattagat ttggaaaatg aaaaaataat
     6781 aaaatcgcat caagaaaact tattacctta gagattccca attgatatct gtacccctga
     6841 tccaagtaag gcctttatga atgtatttta tatttcctaa agaaataggg aatatgccat
     6901 cagtgtaatc acctctattc acagtcattt aatttgaaaa tctaactcat gtctcttgaa
     6961 ttagacattc caattgggat aggtagagtc atgttaaaac aatagaatag caactgccat
     7021 attggtagtt tcactgctca atatgatttc taataagagc agagctttat tggaagctaa
     7081 aactcatttt tctccataga tgattctaac agtttaataa aaaagtataa ttatacttta
     7141 agtgtcaaca gattttgagt ctagaagcaa atttcatttt ccttgtgtta tatctaatat
     7201 ccattgacat gggtaagaaa actaattatg tctttttagt atgatgaaat gtgggcccag
     7261 agtattggaa agaaagcaat agccctattc agtgtccact aggatggata ttgcagaagg
     7321 catgagtttg tccttttttc ccccttacct taaataacac ttcaatagct aactttttcc
     7381 aaaatatcca tttaggccat ctcttatttc ttaaagccaa atatttcgtc cccagtctta
     7441 actctgagtt gcaagatcag gctggttgga agaatcttta tatttagaaa taggatggtg
     7501 agtggtttgt ttattctctt gtaaaattcc tttttcagag acataactgc agggagagtc
     7561 ccaagagaac tgaagaactg gattatatca ttaaacacat ttgtgttgta ggaatttggg
     7621 agtttcattt tgaactgata agatattaga cataaaccct tgttccagcc cacatgtgtc
     7681 atccctgtgt acaaaagctc tctggattct tttttttttt ttgagacgga gtttcgctct
     7741 tatcgtccag gctggagtga gtgtagtggc ttgatctcag ctcactgcaa cctttgcctc
     7801 ccgggctcaa gcgattctcc tgcctcagcc tcccaagtag ctgggattac aagcatgtgc
     7861 caccatgccc agctaatttt ctgtattttt agtagagacg gggtttcacc atgttggcca
     7921 ggctggtctc gaactcctga cctcaagtga tctgcccgcc tcagcttccc aaaatgctgg
     7981 aattacaggc atgagccatc acgcctagcc tactctctga atttctaaaa gtcagtaggt
     8041 tgaccaaaaa gtctagaaac tggctttaag tcagtatggg acgtacttat aaagagtcca
     8101 tggttttgca cgtttcggta gacaagtaaa tctgagttat ttttcaatga cttaccaata
     8161 tttgaatagt aactaagatc gtcagtgtat ctggacttct ttttttgaag ttctaaaaca
     8221 attatagtag ggatttatta ttttgggcct ccatccagat gtttttccaa gatcattttt
     8281 aaaattcatt tgtcttctgt ttccagataa catactttcc gttctatagg aatcttcact
     8341 gccaatcata gtatctacca gtggctttct tagactattc actccaaagc tgggactgat
     8401 gtcctgccag tagagaatct acagaaataa tttgaatgaa ttaaaaccaa atcttgatag
     8461 caggagacag cttcctgatc tagatgtaca attagagttt aggttggaaa ttactttaaa
     8521 atgtgttttt tggggatgtc ttcaatctct gtgtaaatac ccacatgctt atgcattgta
     8581 aaccaagtgt gtattcctgt gtatgaattt gtagaactga tttctgcttc aagagaagct
     8641 gcacctttaa ttttataagg tcccctccac ctgtaaccct ataaatgtct gtaaataaaa
     8701 cactaaaatt tgtagtgata ggatcaattt gggaatatct gctgagagac caaaaagttc
     8761 atttttttaa gtaccttggt taaagagtaa agattattcc tcttattttt taaagaagaa
     8821 tgcactttaa caaacataga gctgcatggg caattcaaac aaatctgtga agtgcagtac
     8881 ccattcagaa atcacacttc ctgaaaaccg ttcaaaagca gagtccagac gggctgttga
     8941 tctcactgcc tgtaggttga agctcagatt ctgatcaatt ttgagaggag cagggctgct
     9001 tcaaaagagc aatgtgaata cagtcagaag cttcagactg gtctgtaaaa atggcgggtc
     9061 ccgtatttac cactaactag caaaactgac agaaaaactc acagagaaaa aatgtaagaa
     9121 tccttcctgc tggtgtgcac tccttacaat agacttttgc aaatggagtt ttacagtcta
     9181 tatttaaaaa aaattgtatg tttgtaacaa ataaagtatg cagaaaagtg aa
//
