LOCUS       NM_015317               6309 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens pumilio RNA binding family member 2 (PUM2), transcript
            variant 1, mRNA.
ACCESSION   NM_015317
VERSION     NM_015317.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Bai,T. and Liu,N.
  TITLE     RNA-binding protein PUM2 promotes T-cell acute lymphoblastic
            leukemia via competitively binding to RBM5 3'UTR with miR-28-5p
  JOURNAL   Eur J Haematol 110 (5), 498-509 (2023)
   PUBMED   36536516
  REMARK    GeneRIF: RNA-binding protein PUM2 promotes T-cell acute
            lymphoblastic leukemia via competitively binding to RBM5 3'UTR with
            miR-28-5p.
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Enwerem,I.I.I., Elrod,N.D., Chang,C.T., Lin,A., Ji,P., Bohn,J.A.,
            Levdansky,Y., Wagner,E.J., Valkov,E. and Goldstrohm,A.C.
  TITLE     Human Pumilio proteins directly bind the CCR4-NOT deadenylase
            complex to regulate the transcriptome
  JOURNAL   RNA 27 (4), 445-464 (2021)
   PUBMED   33397688
  REMARK    GeneRIF: Human Pumilio proteins directly bind the CCR4-NOT
            deadenylase complex to regulate the transcriptome.
REFERENCE   3  (bases 1 to 6309)
  AUTHORS   Zhang,T., Zhang,P. and Li,H.X.
  TITLE     CAFs-Derived Exosomal miRNA-130a Confers Cisplatin Resistance of
            NSCLC Cells Through PUM2-Dependent Packaging
  JOURNAL   Int J Nanomedicine 16, 561-577 (2021)
   PUBMED   33542625
  REMARK    GeneRIF: CAFs-Derived Exosomal miRNA-130a Confers Cisplatin
            Resistance of NSCLC Cells Through PUM2-Dependent Packaging.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6309)
  AUTHORS   Wolfe,M.B., Schagat,T.L., Paulsen,M.T., Magnuson,B., Ljungman,M.,
            Park,D., Zhang,C., Campbell,Z.T., Goldstrohm,A.C. and
            Freddolino,P.L.
  TITLE     Principles of mRNA control by human PUM proteins elucidated from
            multimodal experiments and integrative data analysis
  JOURNAL   RNA 26 (11), 1680-1703 (2020)
   PUBMED   32753408
  REMARK    GeneRIF: Principles of mRNA control by human PUM proteins
            elucidated from multimodal experiments and integrative data
            analysis.
REFERENCE   5  (bases 1 to 6309)
  AUTHORS   Fox,M., Urano,J. and Reijo Pera,R.A.
  TITLE     Identification and characterization of RNA sequences to which human
            PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind
  JOURNAL   Genomics 85 (1), 92-105 (2005)
   PUBMED   15607425
  REMARK    GeneRIF: PUM2 and DAZL, are capable of binding the same mRNA
            sequences in the 3'UTR of human SDAD1 mRNA.
REFERENCE   6  (bases 1 to 6309)
  AUTHORS   Dazard,J.E., Gal,H., Amariglio,N., Rechavi,G., Domany,E. and
            Givol,D.
  TITLE     Genome-wide comparison of human keratinocyte and squamous cell
            carcinoma responses to UVB irradiation: implications for skin and
            epithelial cancer
  JOURNAL   Oncogene 22 (19), 2993-3006 (2003)
   PUBMED   12771951
REFERENCE   7  (bases 1 to 6309)
  AUTHORS   Jaruzelska,J., Kotecki,M., Kusz,K., Spik,A., Firpo,M. and Reijo
            Pera,R.A.
  TITLE     Conservation of a Pumilio-Nanos complex from Drosophila germ plasm
            to human germ cells
  JOURNAL   Dev Genes Evol 213 (3), 120-126 (2003)
   PUBMED   12690449
REFERENCE   8  (bases 1 to 6309)
  AUTHORS   Moore,F.L., Jaruzelska,J., Fox,M.S., Urano,J., Firpo,M.T.,
            Turek,P.J., Dorfman,D.M. and Pera,R.A.
  TITLE     Human Pumilio-2 is expressed in embryonic stem cells and germ cells
            and interacts with DAZ (Deleted in AZoospermia) and DAZ-like
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 538-543 (2003)
   PUBMED   12511597
  REMARK    GeneRIF: PUM2 as a component of conserved cellular machinery that
            may be required for germ cell development.
REFERENCE   9  (bases 1 to 6309)
  AUTHORS   Nakayama,M., Kikuno,R. and Ohara,O.
  TITLE     Protein-protein interactions between large proteins: two-hybrid
            screening using a functionally classified library composed of long
            cDNAs
  JOURNAL   Genome Res 12 (11), 1773-1784 (2002)
   PUBMED   12421765
REFERENCE   10 (bases 1 to 6309)
  AUTHORS   Spassov,D.S. and Jurecic,R.
  TITLE     Cloning and comparative sequence analysis of PUM1 and PUM2 genes,
            human members of the Pumilio family of RNA-binding proteins
  JOURNAL   Gene 299 (1-2), 195-204 (2002)
   PUBMED   12459267
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007041.3, BG717417.1,
            AK093847.1 and AF315591.1.
            
            On May 21, 2020 this sequence version replaced NM_015317.4.
            
            Summary: This gene encodes a protein that belongs to a family of
            RNA-binding proteins. The encoded protein functions as a
            translational repressor during embryonic development and cell
            differentiation. This protein is also thought to be a positive
            regulator of cell proliferation in adipose-derived stem cells.
            Alternate splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.3006346.1, AK093847.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361078.7/ ENSP00000354370.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC007041.3         114492-114492       c
            2-31                BG717417.1         12-41
            32-32               AC007041.3         114461-114461       c
            33-142              BG717417.1         42-151
            143-186             AK093847.1         1-44
            187-187             AC007041.3         114306-114306       c
            188-2260            AK093847.1         46-2118
            2261-2261           AC007041.3         27071-27071         c
            2262-3565           AK093847.1         2120-3423
            3566-3567           AC007041.3         15127-15128         c
            3568-3736           AK093847.1         3427-3595
            3737-6309           AF315591.1         3494-6066
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p24.1"
     gene            1..6309
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /note="pumilio RNA binding family member 2"
                     /db_xref="GeneID:23369"
                     /db_xref="HGNC:HGNC:14958"
                     /db_xref="MIM:607205"
     exon            1..248
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            249..317
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..3461
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pumilio-2; pumilio homolog 2"
                     /codon_start=1
                     /product="pumilio homolog 2 isoform 1"
                     /protein_id="NP_056132.1"
                     /db_xref="CCDS:CCDS1698.1"
                     /db_xref="GeneID:23369"
                     /db_xref="HGNC:HGNC:14958"
                     /db_xref="MIM:607205"
                     /translation="MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDE
                     WRETAWGASHHSMSQPIMVQRRSGQGFHGNSEVNAILSPRSESGGLGVSMVEYVLSSS
                     PADKLDSRFRKGNFGTRDAETDGPEKGDQKGKASPFEEDQNRDLKQGDDDDSKINGRG
                     LPNGMDADCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANKPLVEEFSN
                     PETQNLDAMEQVGLESLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTNALTVQQL
                     TAAQQQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAAT
                     LAGPAVVPPQYYGVPWGVYPANLFQQQAAAAANNTASQQAASQAQPGQQQVLRAGAGQ
                     RPLTPNQGQQGQQAESLAAAAAANPTLAFGQGLATGMPGYQVLAPTAYYDQTGALVVG
                     PGARTGLGAPVRLMAPTPVLISSAAAQAAAAAAAGGTASSLTGSTNGLFRPIGTQPPQ
                     QQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLGAAIGSA
                     LSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIGM
                     PLPSQTPGHSLTPPPSLSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSASSTSSL
                     FSSSSQLFPPSRLRYNRSDIMPSGRSRLLEDFRNNRFPNLQLRDLIGHIVEFSQDQHG
                     SRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATR
                     IRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGNHVVQKCI
                     ECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQL
                     VQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAER
                     ALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLR
                     KYTYGKHILAKLEKYYLKNSPDLGPIGGPPNGML"
     exon            318..426
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            427..614
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..784
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..1055
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1149
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1275
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1418
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1557
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1701
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1986
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2223
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2491
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2621
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2750
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            2751..2888
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            2889..3014
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3136
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            3137..3329
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     exon            3330..6309
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6274..6279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /note="hexamer: AATGAA"
     polyA_site      6309
                     /gene="PUM2"
                     /gene_synonym="PUMH2; PUML2"
                     /note="major polyA site"
ORIGIN      
        1 aggggagggc aggcggcggt ggcagccatg ttgttgtgag tctctgtgtc tcaccctcgg
       61 tacttcggtg gttcggagga ggagggggag gagaaggagg aggtggggtg gggggggagg
      121 gtggtaggtg gagaaagaag acattgccgc caccgaaggc ggaggagggg gaggaccgcc
      181 aagagcgacg ccgccgtgtg cccaacggtc ggtgtccccg gctgaggcag cgcggccgca
      241 gcagccctgt ttgatctgtg gatgaaatga atcatgattt tcaagctctt gcattagaat
      301 ctcggggaat gggagagctt ttgcctacca aaaagttttg ggaacctgat gattcaacaa
      361 aagatggaca aaaaggcata tttcttgggg atgatgaatg gagagagact gcatggggag
      421 cttctcacca ttcaatgtcc cagcctatta tggtacagag aagatctgga cagggttttc
      481 atggaaacag tgaagtaaat gcaatactgt ctccgcgatc agaaagtgga ggccttggtg
      541 tgagcatggt agaatatgta ttaagttctt ctcctgctga taaattggat tctcgattta
      601 ggaagggaaa ttttggcact agagatgctg aaacagatgg acctgagaaa ggagatcaaa
      661 aaggcaaggc ttctccattt gaggaggacc aaaacagaga tcttaaacaa ggagatgatg
      721 atgattctaa aataaatggc agaggtttgc caaatggaat ggatgccgat tgcaaagatt
      781 ttaatcgtac tcctggaagt cgtcaagcct ctccaactga agtagttgag cgcttgggcc
      841 ccaatactaa tccctcagaa ggactggggc ctcttcctaa tcctacagct aataaaccac
      901 ttgttgaaga attttcaaat cctgaaactc agaatctgga tgccatggaa caagttggtc
      961 tggaatcctt acagtttgac tatcctggta atcaggtacc aatggactct tcaggagcta
     1021 ctgtaggcct ttttgactac aattcccagc agcagctctt tcagaggact aatgcactaa
     1081 cagttcaaca gttaactgca gctcaacagc agcaatatgc attagcagca gctcagcagc
     1141 cacatatagc tggtgtattc tcagcaggcc ttgctccagc tgcatttgtg ccaaatccat
     1201 acattattag tgctgctcct ccagggaccg atccgtatac tgcagcagga ttggctgcag
     1261 cagctacatt agcaggtcca gcagtggttc cacctcagta ttacggcgtt ccatgggggg
     1321 tgtatccagc caacttattt cagcagcaag ctgcagctgc ggcaaataac acagccagtc
     1381 agcaagcagc atcacaagct cagcctggac agcaacaggt tctccgtgct ggagcaggtc
     1441 agcgtcctct tactcccaat cagggtcagc aagggcagca agcagaatca cttgcggcag
     1501 ctgcagcagc aaatccaaca ttggcttttg gtcagggtct tgctactggc atgccaggct
     1561 atcaagtact agctccaact gcctattatg atcagactgg tgccttagtg gttggccctg
     1621 gagcaaggac tggccttgga gctccagttc ggttaatggc tccaacacct gttttaatta
     1681 gttcagcagc agcacaagct gcagcagcag cagcagctgg aggaactgca agtagcctta
     1741 caggcagcac aaatggtctg tttcggccaa ttggcactca gccaccacag cagcagcaac
     1801 agcagccaag cactaatctg caatctaatt cattttatgg aagcagttct ttgactaata
     1861 gctcccagag tagttcttta ttttctcatg gacctggtca acctggaagt acatctcttg
     1921 gctttggaag tggtaactct ttgggtgctg ctataggctc agccctcagt ggatttggtt
     1981 catcagttgg cagttctgca agtagtagtg ccacaaggag agagtctcta tctactagct
     2041 ctgacttgta caaaagatct agtagcagcc tagcacccat agggcaacca ttttacaata
     2101 gtctgggatt ttcctcctct ccaagtccaa taggcatgcc tctgccaagc caaactccag
     2161 gacattcact tacgccaccg ccatcacttt catcacatgg atcctcatcc agtttgcatt
     2221 taggaggact gacaaatggt agtggtcgat atatctctgc agcacctgga gcagaagcaa
     2281 aatatcgaag tgcttcaagc acttccagtc tatttagctc cagcagccag ctctttcctc
     2341 cttcccggct tcggtataat aggtctgata ttatgccttc tggccgcagt agattattgg
     2401 aagatttcag aaacaaccgc ttcccaaacc ttcagcttag agacttgatt ggacatatag
     2461 ttgagttttc tcaagaccag catggttcta gattcataca gcaaaaacta gagagagcta
     2521 ctccagctga gcgacagatg gtatttaatg aaattctgca agcagcctat caattaatga
     2581 ctgatgtttt tggcaactat gttatacaga agttttttga gtttgggagt ctggatcaaa
     2641 aattagccct ggctactcgt attcgtggtc atgttctacc cttagccttg cagatgtatg
     2701 gctgccgcgt tattcagaaa gcattagaat ctatttcttc tgaccagcag agtgaaatgg
     2761 taaaggagct ggatggtcat gtgctcaaat gtgtgaaaga tcagaatgga aaccatgttg
     2821 tacaaaaatg tatcgaatgt gttcagccac agtcactaca gttcatcatt gatgctttca
     2881 agggacaagt atttgtgctt tcaactcatc cttatggctg cagagtaatt cagcgcatcc
     2941 tagagcattg cactgcagaa cagaccttac ctatcttaga agaactccac caacatacag
     3001 agcagttggt acaggatcag tatggcaatt atgttattca gcatgtactg gaacacggtc
     3061 gacctgaaga caagagcaaa attgtttccg aaatcagggg aaaggtttta gccctgagtc
     3121 aacacaaatt tgccagcaat gtagtagaaa agtgtgttac tcatgcctcc cgtgctgaga
     3181 gagctttact gattgacgag gtttgctgcc agaatgatgg tcctcacagt gccttataca
     3241 ccatgatgaa ggaccagtat gccaattacg tggttcaaaa gatgattgat atggctgaac
     3301 ctgctcagag aaagataatc atgcacaaga ttcgacctca cattactact ttgcgcaaat
     3361 acacatacgg gaagcatata ctggccaagt tggaaaagta ttatttgaag aatagcccgg
     3421 acctaggacc tattggagga ccaccaaatg gaatgctgta aattacagga gcaagagaaa
     3481 gaagataatt taaccatgtg aaaagaattt ttttgtgtgt gaattatcaa aacacaactc
     3541 aactatgaat cttcaatttt tttttaaagc aaaactattt attgacttta ttcatccatt
     3601 tgtaaatttt ttaaggttct tgtgtatatt tggggggtgg gggatgaatt ataaattata
     3661 ttcagccctg agtggagacc tatcagattg gattgctggc aaagcacaga atgcctgtat
     3721 atgatgtaac tgtatcaaaa ataaaaagct gtcacatatt ttgtaaattt ttaccttgta
     3781 aagtcacaaa aatagttttt aaaggaaaaa gtacagtatt cttttaataa actggctcac
     3841 agtctggtag gtctacaacc ccatagcaca acaggtttat agagatgtat atagaattat
     3901 agtccttatt tttttccttt gcgtgaaacc ttttataaca gattaacaat caactgcata
     3961 aatattatta atattttaaa aagagttaag ttgtattttg ataattcaca aactatcatg
     4021 caaataacga gtaagtagac aagaataaag tggtttgaga tgaaaagaac ctaacattat
     4081 ttacagtaga tgtggtttta atacaattac tgccctaaaa tgtctctggc aatgtacaga
     4141 aatattgtat atacttacat atgtaattgt tgtaagagtt aaatacaaaa tcatggtgac
     4201 acttccaatt aagtgcacta aatgaaaagt taagtcactt attaactttt cagtttggtt
     4261 tgcaatgaga aagagtggaa atttgtattt tgttttgctt atagaattac agacatgttg
     4321 aggaagtgtt gagctttatt ttgctttttc atagaggcag aaagtaggaa ccagatagag
     4381 atgaaaaggg gccactgaaa agtgaatttg atagctcagc atttaagcat gattacatat
     4441 tcagatagct ctttttgctt tctataaata tatgcattgt gtgtgtagta atagatgtaa
     4501 gtttacactt tgaaaggaaa tcttgtttca atgtttatta taaaagcctt gctaatttag
     4561 tagtgatgct ttccttggtt gtacaggtgt acatttgtaa accttcatgc tgtaaatgga
     4621 atttgtttta tctctttggg atacatttgc attttagtgt acatttacgt ccctgccctc
     4681 tttgacctgg caatatagtg ttgtataatg taaatttatt tctccaaatc gagagtgatt
     4741 ttttaaaaat tttttatctt tatatggttt cagaagtatg aaccagcttt ctttttatta
     4801 ttgtgagatc attttgtttt ataacatagt tgttgactgt taatatggac ctgctagaat
     4861 ttggatcact ttcaattgaa gtcagggtat tgtgcataat agaaagtatt ggactgagat
     4921 atttggttac catggaggcc aatgcttttt tcatcttatt aaatgtgatg tgactttttt
     4981 ctttgtacag aagagtactg tatttttgaa tagcctactc ccaagtaaga gcaaatctgt
     5041 atgataacat tttttcctct ggacataaga cataacagta acacgatgta catttacaag
     5101 cggccttatg tacatttccc aacaatcttt ttaaggcaaa attgtgacca tatgtgtata
     5161 attaaaatcg tttttaatcc tttgcctatg aaaatatttt ggaaaaaaac ttgctgtgta
     5221 tattcagttt ctgaaagata aagaaagtgc tttgtatttt gttgaagtca gtattttgta
     5281 taaacattta tgttgaccca cttatgttca gtgctgaaaa ctaaaatgaa catgctattc
     5341 tgtcagctga atatggaaga gatctttttt tactagagat ctgcagaaga aacgcaatct
     5401 tctgagcaca atatggaatc taaaggtttt atcacttagt tgttcatatt atgaacctaa
     5461 aaataatggc ataaagtttg gggatgccag gcatactttt tcatgtttgg tgttgagtta
     5521 ttttactttt ctaacccaac attccttggt gagaccatta aatccaaaca cttgtcaccg
     5581 ttccttctca tagtcactct gggtcatcag catgtcccag tcactgcagc aacgccttgt
     5641 gtttgtttca tttttttaaa acccacacaa agccgctgtc tcactttttc ctactttacc
     5701 aacctcagag tatttcggcc cgtatcgaac ttttgttctc agtatcagcc catggtttca
     5761 ggatcaaagc tgtcatgttg gagattggta atggctttcc tgtctttgta cagttgaatt
     5821 cctagtcttc cttcatcctt gccctctgtt ggcacaggca ttatctctgc aattttagaa
     5881 aatgacaagt agagaatact acattgagaa actaaaccct cttcttgggg tcctgatact
     5941 cattcccatt tgtcccagtg ctgacaaccc aatcttccca atactttcag gcctgctcta
     6001 caaaagtacc tgttcttgta gaaattttac agtctgccat tttgggtgcc caccccaatt
     6061 tttacctttt agtaagttgg catgaaattt tggtaaaatc tgaaaatcac atttcagaat
     6121 aaaacaattg ggcaaaacta cctaggcttt actcttgagt gtctcctttt gatagggatt
     6181 gtttctggac cagtttgtct aagtcctggc tcttattggt tcatatgaaa taatgttaac
     6241 ttcacttctt tgtatattat gtataaatta gaaaatgaaa aatgtgtgaa taacattgta
     6301 tgaaataaa
//
