LOCUS       NM_001020658            5385 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript
            variant 1, mRNA.
ACCESSION   NM_001020658
VERSION     NM_001020658.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Muhtadi,R., Stewart,S., Bunert,F., Fatanmi,O.O., Wise,S.Y.,
            Gartner,C., Motzke,S., Ruf,C., Ostheim,P., Schule,S., Schwanke,D.,
            Singh,V.K., Port,M. and Abend,M.
  TITLE     PUM1 and PGK1 are Favorable Housekeeping Genes over Established
            Biodosimetry-related Housekeeping Genes such as HPRT1, ITFG1, DPM1,
            MRPS5, 18S rRNA and Others after Radiation Exposure
  JOURNAL   Radiat Res 201 (5), 487-498 (2024)
   PUBMED   38471523
  REMARK    GeneRIF: PUM1 and PGK1 are Favorable Housekeeping Genes over
            Established Biodosimetry-related Housekeeping Genes such as HPRT1,
            ITFG1, DPM1, MRPS5, 18S rRNA and Others after Radiation Exposure.
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Lu,C., Zheng,F., Pan,L., Han,Q., Wu,J. and Zhang,W.
  TITLE     Suppression of circular RNA serum and glucocorticoid-induced kinase
            1 elevates antioxidant molecules and angiogenesis in trophoblast
            cells to attenuate preeclampsia via microRNA-508-3p to target and
            restrain PUM homolog 1
  JOURNAL   J Obstet Gynaecol Res 50 (3), 322-333 (2024)
   PUBMED   38081797
  REMARK    GeneRIF: Suppression of circular RNA serum and
            glucocorticoid-induced kinase 1 elevates antioxidant molecules and
            angiogenesis in trophoblast cells to attenuate preeclampsia via
            microRNA-508-3p to target and restrain PUM homolog 1.
REFERENCE   3  (bases 1 to 5385)
  AUTHORS   Wang,H., Zhou,Z., Zhang,J., Hao,T., Wang,P., Wu,P., Su,R., Yang,H.,
            Deng,G., Chen,S., Gu,L., He,Y., Zeng,L., Zhang,C. and Yin,S.
  TITLE     Pumilio1 regulates NPM3/NPM1 axis to promote PD-L1-mediated immune
            escape in gastric cancer
  JOURNAL   Cancer Lett 581, 216498 (2024)
   PUBMED   38029539
  REMARK    GeneRIF: Pumilio1 regulates NPM3/NPM1 axis to promote
            PD-L1-mediated immune escape in gastric cancer.
REFERENCE   4  (bases 1 to 5385)
  AUTHORS   Caponnetto,A., Ferrara,C., Fazzio,A., Agosta,N., Scribano,M.,
            Vento,M.E., Borzi,P., Barbagallo,C., Stella,M., Ragusa,M.,
            Scollo,P., Barbagallo,D., Purrello,M., Di Pietro,C. and
            Battaglia,R.
  TITLE     A Circular RNA Derived from the Pumilio 1 Gene Could Regulate PTEN
            in Human Cumulus Cells
  JOURNAL   Genes (Basel) 15 (1), 124 (2024)
   PUBMED   38275605
  REMARK    GeneRIF: A Circular RNA Derived from the Pumilio 1 Gene Could
            Regulate PTEN in Human Cumulus Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5385)
  AUTHORS   Yin,S., Liu,H., Zhou,Z., Xu,X., Wang,P., Chen,W., Deng,G., Wang,H.,
            Yu,H., Gu,L., Huo,M., Li,M., Zeng,L., He,Y. and Zhang,C.
  TITLE     PUM1 Promotes Tumor Progression by Activating DEPTOR-Meditated
            Glycolysis in Gastric Cancer
  JOURNAL   Adv Sci (Weinh) 10 (27), e2301190 (2023)
   PUBMED   37469018
  REMARK    GeneRIF: PUM1 Promotes Tumor Progression by Activating
            DEPTOR-Meditated Glycolysis in Gastric Cancer.
REFERENCE   6  (bases 1 to 5385)
  AUTHORS   Wang,X., McLachlan,J., Zamore,P.D. and Hall,T.M.
  TITLE     Modular recognition of RNA by a human pumilio-homology domain
  JOURNAL   Cell 110 (4), 501-512 (2002)
   PUBMED   12202039
REFERENCE   7  (bases 1 to 5385)
  AUTHORS   Wang,X., Zamore,P.D. and Hall,T.M.
  TITLE     Crystal structure of a Pumilio homology domain
  JOURNAL   Mol Cell 7 (4), 855-865 (2001)
   PUBMED   11336708
REFERENCE   8  (bases 1 to 5385)
  AUTHORS   Kirchhausen,T.
  TITLE     Three ways to make a vesicle
  JOURNAL   Nat Rev Mol Cell Biol 1 (3), 187-198 (2000)
   PUBMED   11252894
  REMARK    Review article
            Erratum:[Nat Rev Mol Cell Biol 2001 Mar;2(3):216]
REFERENCE   9  (bases 1 to 5385)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
REFERENCE   10 (bases 1 to 5385)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL699026.1, AK222605.1,
            AF315592.1 and CN402209.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001020658.1.
            
            Summary: This gene encodes a member of the PUF family,
            evolutionarily conserved RNA-binding proteins related to the
            Pumilio proteins of Drosophila and the fem-3 mRNA binding factor
            proteins of C. elegans. The encoded protein contains a
            sequence-specific RNA binding domain comprised of eight repeats and
            N- and C-terminal flanking regions, and serves as a translational
            regulator of specific mRNAs by binding to their 3' untranslated
            regions. The evolutionarily conserved function of the encoded
            protein in invertebrates and lower vertebrates suggests that the
            human protein may be involved in translational regulation of
            embryogenesis, and cell development and differentiation.
            Alternatively spliced transcript variants encoding different
            isoforms have been described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK222605.1, SRR1803617.118284.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000426105.7/ ENSP00000391723.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AL699026.1         1-22
            23-759              AK222605.1         1-737
            760-760             AF315592.1         742-742
            761-1517            AK222605.1         739-1495
            1518-1518           AF315592.1         1500-1500
            1519-2449           AK222605.1         1497-2427
            2450-2450           AF315592.1         2432-2432
            2451-4057           AK222605.1         2429-4035
            4058-4249           AF315592.1         4034-4225
            4250-4251           CN402209.1         179-180
            4252-5385           AF315592.1         4226-5359
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.2"
     gene            1..5385
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /note="pumilio RNA binding family member 1"
                     /db_xref="GeneID:9698"
                     /db_xref="HGNC:HGNC:14957"
                     /db_xref="MIM:607204"
     exon            1..102
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            103..476
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..3680
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pumilio-1; pumilio homolog 1"
                     /codon_start=1
                     /product="pumilio homolog 1 isoform 1"
                     /protein_id="NP_001018494.1"
                     /db_xref="CCDS:CCDS44099.1"
                     /db_xref="GeneID:9698"
                     /db_xref="HGNC:HGNC:14957"
                     /db_xref="MIM:607204"
                     /translation="MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGS
                     QAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGG
                     YNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLPGKKFWETDESSK
                     DGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLG
                     VSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVM
                     DKTNGLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPV
                     EDFSNMESQSVPLDPMEHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPN
                     ALAVQQLTAAQQQQYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTDPYTAGLAAAAT
                     LGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGA
                     SQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALV
                     VNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLG
                     TQQPQPQPQQQPNNNLASSSFYGNNSLNSNSQSSSLFSQGSAQPANTSLGFGSSSSLG
                     ATLGSALGGFGTAVANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGLSFSSS
                     PGPVGMPLPSQGPGHSQTPPPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRSA
                     SSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEF
                     SQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQK
                     LALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGHVLKCVKDQNG
                     NHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEE
                     LHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCV
                     THASRTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKI
                     RPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII"
     exon            477..545
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            546..654
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            655..833
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            834..1000
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1271
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1365
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1467
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1619
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1758
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1902
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2199
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2436
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2704
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2834
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2969
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..3107
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            3108..3233
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3355
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3548
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     exon            3549..5385
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5365..5370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /note="hexamer: AATACA"
     polyA_site      5385
                     /gene="PUM1"
                     /gene_synonym="HSPUM; NEDMSF; PUMH; PUMH1; PUML1; SCA47"
                     /note="major polyA site"
ORIGIN      
        1 agtgggccgc catgttgtcg gagtgaaagg taagggggag cgagagcgcc agagagagaa
       61 gatcgggggg ctgaaatcca tcttcatcct accgctccgc ccgtgttggt ggaatgagcg
      121 ttgcatgtgt cttgaagaga aaagcagtgc tttggcagga ctctttcagc ccccacctga
      181 aacatcaccc tcaagaacca gctaatccca acatgcctgt tgttttgaca tctggaacag
      241 ggtcgcaagc gcagccacaa ccagctgcaa atcaggctct tgcagctggg actcactcca
      301 gccctgtccc aggatctata ggagttgcag gccgttccca ggacgacgct atggtggact
      361 acttctttca gaggcagcat ggtgagcagc ttgggggagg aggaagtgga ggaggcggct
      421 ataataatag caaacatcga tggcctactg gggataacat tcatgcagaa catcaggtgc
      481 gttccatgga tgaactgaat catgattttc aagcacttgc tctggaggga agagcgatgg
      541 gagagcagct cttgccaggt aaaaagtttt gggaaacaga tgaatccagc aaagatggac
      601 caaaaggaat attcctgggt gatcaatggc gagacagtgc ctggggaaca tcagatcatt
      661 cagtttccca gccaatcatg gtgcagagaa gacctggtca gagtttccat gtgaacagtg
      721 aggtcaattc tgtactgtcc ccacgatcgg agagtggggg actaggcgtt agcatggtgg
      781 agtatgtgtt gagctcatcc ccgggcgatt cctgtctaag aaaaggagga tttggcccaa
      841 gggatgcaga cagtgatgaa aacgacaaag gtgaaaagaa gaacaagggt acgtttgatg
      901 gagataagct aggagatttg aaggaggagg gtgatgtgat ggacaagacc aatggtttac
      961 cagtgcagaa tgggattgat gcagacgtca aagattttag ccgtacccct ggtaattgcc
     1021 agaactctgc taatgaagtg gatcttctgg gtccaaacca gaatggttct gagggcttag
     1081 cccagctgac cagcaccaat ggtgccaagc ctgtggagga tttctccaac atggagtccc
     1141 agagtgtccc cttggacccc atggaacatg tgggcatgga gcctcttcag tttgattatt
     1201 caggcacgca ggtacctgtg gactcagcag cagcaactgt gggacttttt gactacaatt
     1261 ctcaacaaca gctgttccaa agacctaatg cgcttgctgt ccagcagttg acagctgctc
     1321 agcagcagca gtatgcactg gcagctgctc atcagccgca catcggttta gctcccgctg
     1381 cgtttgtccc caatccatac atcatcagcg ctgctccccc agggacggac ccctacacag
     1441 ctggattggc tgcagcagcg acactaggcc cagctgtggt ccctcaccag tattatggag
     1501 ttactccctg gggagtctac cctgccagtc ttttccagca gcaagctgcc gctgccgctg
     1561 cagcaactaa ttcagctaat caacagacca ccccacaggc tcagcaagga cagcagcagg
     1621 ttctccgtgg aggagccagc caacgtcctt tgaccccaaa ccagaaccag cagggacagc
     1681 aaacggatcc ccttgtggca gctgcagcag tgaattctgc ccttgcattt ggacaaggtc
     1741 tggcagcagg catgccaggt tatccggtgt tggctcctgc tgcttactat gaccaaactg
     1801 gtgcccttgt agtgaatgca ggcgcgagaa atggtcttgg agctcctgtt cgacttgtag
     1861 ctcctgcccc agtcatcatt agttcctcag ctgcacaagc agctgttgca gcagccgcag
     1921 cttcagcaaa tggagcagct ggtggtcttg ctggaacaac aaatggacca tttcgccctt
     1981 taggaacaca gcagcctcag ccccagcccc agcagcagcc caataacaac ctggcatcca
     2041 gttctttcta cggcaacaac tctctgaaca gcaattcaca gagcagctcc ctcttctccc
     2101 agggctctgc ccagcctgcc aacacatcct tgggattcgg aagtagcagt tctctcggcg
     2161 ccaccctggg atccgccctt ggagggtttg gaacagcagt tgcaaactcc aacactggca
     2221 gtggctcccg ccgtgactcc ctgactggca gcagtgacct ttataagagg acatcgagca
     2281 gcttgacccc cattggacac agtttttata acggccttag cttttcctcc tctcctggac
     2341 ccgtgggcat gcctctccct agtcagggac caggacattc acagacacca cctccttccc
     2401 tctcttcaca tggatcctct tcaagcttaa acctgggagg actcacgaat ggcagtggaa
     2461 gatacatctc tgctgctcca ggcgctgaag ccaagtaccg cagtgcaagc agcgcctcca
     2521 gcctcttcag cccgagcagc actcttttct cttcctctcg tttgcgatat ggaatgtctg
     2581 atgtcatgcc ttctggcagg agcaggcttt tggaagattt tcgaaacaac cggtacccca
     2641 atttacaact gcgggagatt gctggacata taatggaatt ttcccaagac cagcatgggt
     2701 ccagattcat tcagctgaaa ctggagcgtg ccacaccagc tgagcgccag cttgtcttca
     2761 atgaaatcct ccaggctgcc taccaactca tggtggatgt gtttggtaat tacgtcattc
     2821 agaagttctt tgaatttggc agtcttgaac agaagctggc tttggcagaa cggattcgag
     2881 gccacgtcct gtcattggca ctacagatgt atggctgccg tgttatccag aaagctcttg
     2941 agtttattcc ttcagaccag caggtaatta atgagatggt tcgggaacta gatggccatg
     3001 tcttgaagtg tgtgaaagat cagaatggca atcacgtggt tcagaaatgc attgaatgtg
     3061 tacagcccca gtctttgcaa tttatcatcg atgcgtttaa gggacaggta tttgccttat
     3121 ccacacatcc ttatggctgc cgagtgattc agagaatcct ggagcactgt ctccctgacc
     3181 agacactccc tattttagag gagcttcacc agcacacaga gcagcttgta caggatcaat
     3241 atggaaatta tgtaatccaa catgtactgg agcacggtcg tcctgaggat aaaagcaaaa
     3301 ttgtagcaga aatccgaggc aatgtacttg tattgagtca gcacaaattt gcaagcaatg
     3361 ttgtggagaa gtgtgttact cacgcctcac gtacggagcg cgctgtgctc atcgatgagg
     3421 tgtgcaccat gaacgacggt ccccacagtg ccttatacac catgatgaag gaccagtatg
     3481 ccaactacgt ggtccagaag atgattgacg tggcggagcc aggccagcgg aagatcgtca
     3541 tgcataagat ccggccccac atcgcaactc ttcgtaagta cacctatggc aagcacattc
     3601 tggccaagct ggagaagtac tacatgaaga acggtgttga cttagggccc atctgtggcc
     3661 cccctaatgg tatcatctga ggcagtgtca cccgctgttc cctcattccc gctgacctca
     3721 ctggcccact ggcaaatcca accagcaacc agaaatgttc tagtgtagag tctgagacgg
     3781 gcaagtggtt gctccaggat tactccctcc tccaaaaaag gaatcaaatc cacgagtgga
     3841 aaagcctttg taaatttaat tttattacac ataacatgta ctattttttt taattgacta
     3901 attgccctgc tgttttactg gtgtatagga tacttgtaca taggtaacca atgtacatgg
     3961 gaggccacat attttgttca ctgttgtatc tatatttcac atgtggaaac tttcagggtg
     4021 gttggtttaa caaaaaaaaa aagctttaaa aaaaaaagaa aaaaaggaaa aggtttttag
     4081 ctcatttgcc tggccggcaa gttttgcaaa tagctcttcc ccacctcctc attttagtaa
     4141 aaaacaaaca aaaacaaaaa aacctgagaa gtttgaattg tagttaaatg accccaaact
     4201 ggcatttaac actgtttata aaaaatatat atatatatat atatatatat aatgaaaaag
     4261 gtttcagagt tgctaaagct tcagtttgtg acattaagtt tatgaaattc taaaaaatgc
     4321 cttttttgga gactatatta tgctgaagaa ggctgttcgt gaggaggaga tgcgagcacc
     4381 cagaacgtct tttgaggctg ggcgggtgtg attgtttact gcctactgga tttttttcta
     4441 ttaacattga aaggtaaaat ctgattattt agcatgagaa aaaaaaatcc aactctgctt
     4501 ttggtcttgc ttctataaat atatagtgta tacttggtgt agactttgca tatatacaaa
     4561 tttgtagtat tttcttgttt tgatgtctaa tctgtatcta taatgtaccc tagtagtcga
     4621 acatactttt gattgtacaa ttgtacattt gtatacctgt aatgtaaatg tggagaagtt
     4681 tgaatcaaca taaacacgtt ttttggtaag aaaagagaat tagccagccc tgtgcattca
     4741 gtgtatattc tcacctttta tggtcgtagc atatagtgtt gtatattgta aattgtaatt
     4801 tcaaccagaa gtaaattttt ttcttttgaa ggaataaatg ttctttatac agcctagtta
     4861 atgtttaaaa agaaaaaaat agcttggttt tatttgtcat ctagtctcaa gtatagcgag
     4921 attctttcta aatgttattc aagattgagt tctcactagt gtttttttaa tcctaaaaaa
     4981 gtaatgtttt gattttgtga cagtcaaaag gacgtgcaaa agtctagcct tgcccgagct
     5041 ttccttacaa tcagagcccc tctcaccttg taaagtgtga atcgcccttc ccttttgtac
     5101 agaagatgaa ctgtattttg cattttgtct acttgtaagt gaatgtaaca tactgtcaat
     5161 tttccttgtt tgaatataga attgtaacac tacacggtgt acatttccag agccttgtgt
     5221 atatttccaa tgaacttttt tgcaagcaca cttgtaacca tatgtgtata attaacaaac
     5281 ctgtgtatgc ttatgcctgg gcaactattt tttgtaactc ttgtgtagat tgtctctaaa
     5341 caatgtgtga tctttatttt gaaaaataca gaactttgga atctg
//
