LOCUS       NM_030667               6128 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens protein tyrosine phosphatase receptor type O (PTPRO),
            transcript variant 1, mRNA.
ACCESSION   NM_030667
VERSION     NM_030667.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Tan,S., Chi,H., Wang,P., Zhao,R., Zhang,Q., Gao,Z., Xue,H., Tang,Q.
            and Li,G.
  TITLE     Protein tyrosine phosphatase receptor type O serves as a key
            regulator of insulin resistance-induced alpha-synuclein aggregation
            in Parkinson's disease
  JOURNAL   Cell Mol Life Sci 81 (1), 403 (2024)
   PUBMED   39276174
  REMARK    GeneRIF: Protein tyrosine phosphatase receptor type O serves as a
            key regulator of insulin resistance-induced alpha-synuclein
            aggregation in Parkinson's disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Dai,Y., Shi,S., Liu,H., Zhou,H., Ding,W., Liu,C., Jin,L., Xie,W.,
            Kong,H. and Zhang,Q.
  TITLE     Protein tyrosine phosphatase PTPRO represses lung adenocarcinoma
            progression by inducing mitochondria-dependent apoptosis and
            restraining tumor metastasis
  JOURNAL   Cell Death Dis 15 (1), 11 (2024)
   PUBMED   38182570
  REMARK    GeneRIF: Protein tyrosine phosphatase PTPRO represses lung
            adenocarcinoma progression by inducing mitochondria-dependent
            apoptosis and restraining tumor metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6128)
  AUTHORS   Dong,H., Xie,C., Yao,Z., Zhao,R., Lin,Y., Luo,Y., Chen,S., Qin,Y.,
            Chen,Y. and Zhang,H.
  TITLE     PTPRO-related CD8+ T-cell signatures predict prognosis and
            immunotherapy response in patients with breast cancer
  JOURNAL   Front Immunol 13, 947841 (2022)
   PUBMED   36003382
  REMARK    GeneRIF: PTPRO-related CD8(+) T-cell signatures predict prognosis
            and immunotherapy response in patients with breast cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6128)
  AUTHORS   Jacob,S.T. and Motiwala,T.
  TITLE     Epigenetic regulation of protein tyrosine phosphatases: potential
            molecular targets for cancer therapy
  JOURNAL   Cancer Gene Ther 12 (8), 665-672 (2005)
   PUBMED   15803146
  REMARK    Review article
REFERENCE   5  (bases 1 to 6128)
  AUTHORS   Wang,Z., Bhattacharya,N., Meyer,M.K., Seimiya,H., Tsuruo,T.,
            Tonani,J.A. and Magnuson,N.S.
  TITLE     Pim-1 negatively regulates the activity of PTP-U2S phosphatase and
            influences terminal differentiation and apoptosis of monoblastoid
            leukemia cells
  JOURNAL   Arch Biochem Biophys 390 (1), 9-18 (2001)
   PUBMED   11368509
REFERENCE   6  (bases 1 to 6128)
  AUTHORS   Pixley,F.J., Lee,P.S., Condeelis,J.S. and Stanley,E.R.
  TITLE     Protein tyrosine phosphatase phi regulates paxillin tyrosine
            phosphorylation and mediates colony-stimulating factor 1-induced
            morphological changes in macrophages
  JOURNAL   Mol Cell Biol 21 (5), 1795-1809 (2001)
   PUBMED   11238916
REFERENCE   7  (bases 1 to 6128)
  AUTHORS   Aguiar,R.C., Yakushijin,Y., Kharbanda,S., Tiwari,S., Freeman,G.J.
            and Shipp,M.A.
  TITLE     PTPROt: an alternatively spliced and developmentally regulated
            B-lymphoid phosphatase that promotes G0/G1 arrest
  JOURNAL   Blood 94 (7), 2403-2413 (1999)
   PUBMED   10498613
REFERENCE   8  (bases 1 to 6128)
  AUTHORS   Taniguchi,Y., London,R., Schinkmann,K., Jiang,S. and Avraham,H.
  TITLE     The receptor protein tyrosine phosphatase, PTP-RO, is upregulated
            during megakaryocyte differentiation and Is associated with the
            c-Kit receptor
  JOURNAL   Blood 94 (2), 539-549 (1999)
   PUBMED   10397721
REFERENCE   9  (bases 1 to 6128)
  AUTHORS   Seimiya,H., Sawabe,T., Inazawa,J. and Tsuruo,T.
  TITLE     Cloning, expression and chromosomal localization of a novel gene
            for protein tyrosine phosphatase (PTP-U2) induced by various
            differentiation-inducing agents
  JOURNAL   Oncogene 10 (9), 1731-1738 (1995)
   PUBMED   7753550
REFERENCE   10 (bases 1 to 6128)
  AUTHORS   Wiggins,R.C., Wiggins,J.E., Goyal,M., Wharram,B.L. and Thomas,P.E.
  TITLE     Molecular cloning of cDNAs encoding human GLEPP1, a membrane
            protein tyrosine phosphatase: characterization of the GLEPP1
            protein distribution in human kidney and assignment of the GLEPP1
            gene to human chromosome 12p12-p13
  JOURNAL   Genomics 27 (1), 174-181 (1995)
   PUBMED   7665166
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR000351.1, BC126201.1 and
            AC007542.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_030667.2.
            
            Summary: This gene encodes a member of the R3 subtype family of
            receptor-type protein tyrosine phosphatases. These proteins are
            localized to the apical surface of polarized cells and may have
            tissue-specific functions through activation of Src family kinases.
            This gene contains two distinct promoters, and alternatively
            spliced transcript variants encoding multiple isoforms have been
            observed. The encoded proteins may have multiple isoform-specific
            and tissue-specific functions, including the regulation of
            osteoclast production and activity, inhibition of cell
            proliferation and facilitation of apoptosis. This gene is a
            candidate tumor suppressor, and decreased expression of this gene
            has been observed in several types of cancer. [provided by RefSeq,
            May 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.125443.1,
                                           SRR1803615.275739.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281171.9/ ENSP00000281171.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                DR000351.1         234-282
            50-5196             BC126201.1         1-5147
            5197-6128           AC007542.3         145310-146241
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.3"
     gene            1..6128
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /note="protein tyrosine phosphatase receptor type O"
                     /db_xref="GeneID:5800"
                     /db_xref="HGNC:HGNC:9678"
                     /db_xref="MIM:600579"
     exon            1..294
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..3870
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /EC_number="3.1.3.48"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; PTPase U2; osteoclastic transmembrane
                     protein-tyrosine phosphatase; protein tyrosine phosphatase
                     PTP-U2; phosphotyrosine phosphatase U2; PTP phi;
                     glomerular epithelial protein 1"
                     /codon_start=1
                     /product="receptor-type tyrosine-protein phosphatase O
                     isoform a precursor"
                     /protein_id="NP_109592.1"
                     /db_xref="CCDS:CCDS8675.1"
                     /db_xref="GeneID:5800"
                     /db_xref="HGNC:HGNC:9678"
                     /db_xref="MIM:600579"
                     /translation="MGHLPTGIHGARRLLPLLWLFVLFKNATAFHVTVQDDNNIVVSL
                     EASDVISPASVYVVKITGESKNYFFEFEEFNSTLPPPVIFKASYHGLYYIITLVVVNG
                     NVVTKPSRSITVLTKPLPVTSVSIYDYKPSPETGVLFEIHYPEKYNVFTRVNISYWEG
                     KDFRTMLYKDFFKGKTVFNHWLPGMCYSNITFQLVSEATFNKSTLVEYSGVSHEPKQH
                     RTAPYPPQNISVRIVNLNKNNWEEQSGNFPEESFMRSQDTIGKEKLFHFTEETPEIPS
                     GNISSGWPDFNSSDYETTSQPYWWDSASAAPESEDEFVSVLPMEYENNSTLSETEKST
                     SGSFSFFPVQMILTWLPPKPPTAFDGFHIHIEREENFTEYLMVDEEAHEFVAELKEPG
                     KYKLSVTTFSSSGSCETRKSQSAKSLSFYISPSGEWIEELTEKPQHVSVHVLSSTTAL
                     MSWTSSQENYNSTIVSVVSLTCQKQKESQRLEKQYCTQVNSSKPIIENLVPGAQYQVV
                     IYLRKGPLIGPPSDPVTFAIVPTGIKDLMLYPLGPTAVVLSWTRPYLGVFRKYVVEMF
                     YFNPATMTSEWTTYYEIAATVSLTASVRIANLLPAWYYNFRVTMVTWGDPELSCCDSS
                     TISFITAPVAPEITSVEYFNSLLYISWTYGDDTTDLSHSRMLHWMVVAEGKKKIKKSV
                     TRNVMTAILSLPPGDIYNLSVTACTERGSNTSMLRLVKLEPAPPKSLFAVNKTQTSVT
                     LLWVEEGVADFFEVFCQQVGSSQKTKLQEPVAVSSHVVTISSLLPATAYNCSVTSFSH
                     DSPSVPTFIAVSTMVTEMNPNVVVISVLAILSTLLIGLLLVTLIILRKKHLQMARECG
                     AGTFVNFASLERDGKLPYNWRRSIFAFLTLLPSCLWTDYLLAFYINPWSKNGLKKRKL
                     TNPVQLDDFDAYIKDMAKDSDYKFSLQFEELKLIGLDIPHFAADLPLNRCKNRYTNIL
                     PYDFSRVRLVSMNEEEGADYINANYIPGYNSPQEYIATQGPLPETRNDFWKMVLQQKS
                     QIIVMLTQCNEKRRVKCDHYWPFTEEPIAYGDITVEMISEEEQDDWACRHFRINYADE
                     MQDVMHFNYTAWPDHGVPTANAAESILQFVHMVRQQATKSKGPMIIHCSAGVGRTGTF
                     IALDRLLQHIRDHEFVDILGLVSEMRSYRMSMVQTEEQYIFIHQCVQLMWMKKKQQFC
                     ISDVIYENVSKS"
     exon            295..568
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            569..727
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            728..880
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1324
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1486
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1683
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1804
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1998
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2110
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2262
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2263..2383
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2523
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2524..2656
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..2777
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2778..2846
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..2930
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2931..2966
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            2967..3048
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3049..3139
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3216
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3217..3351
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3474
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3629
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3765
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3766..3886
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     exon            3887..6128
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5109..5114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /note="hexamer: AATAAG"
     polyA_site      5143
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
     regulatory      5158..5163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /note="hexamer: AGTAAA"
     polyA_site      5190
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /note="major polyA site"
     regulatory      6107..6112
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
                     /note="hexamer: AATAAA"
     polyA_site      6128
                     /gene="PTPRO"
                     /gene_synonym="GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT;
                     PTPU2; R-PTP-O"
ORIGIN      
        1 agtcattaaa gaacagcagc gcctggcacg ttcttggagg accccgggcg cagaggagga
       61 aagggagcag gcgcaggggg actggaaagg cagcatgcgc tcgccaggag caacctcggc
      121 gcccagggtc tgaggctgca gccccagttc gccattgtga gccgccgccg ggggagtccg
      181 ctagcgcagc cgtgcccccg agtccccgtc cgcgcagcga tggggcacct gcccacgggg
      241 atacacggcg cccgccgcct cctgcctctg ctctggctct ttgtgctgtt caagaatgct
      301 acagctttcc atgtaactgt ccaagatgat aataacatcg ttgtctcatt agaagcttca
      361 gacgtcatca gtccagcatc tgtgtatgtt gtgaagataa ctggtgaatc caaaaattat
      421 ttcttcgaat ttgaggaatt caacagcact ttgcctcctc ctgttatttt caaggccagt
      481 tatcatggcc tttattatat aatcactctg gtagtggtaa atggaaatgt ggtgaccaag
      541 ccatccagat caatcactgt gttaacaaaa cctctacctg taaccagtgt ttccatatat
      601 gactataaac cttctcctga aacaggagtc ctgtttgaaa tacattatcc agaaaaatat
      661 aacgttttca caagagtgaa cattagctac tgggaaggta aagacttccg gacaatgcta
      721 tataaagatt tctttaaggg aaaaacagta tttaatcact ggctgccagg aatgtgttat
      781 agtaatatca cctttcagct ggtatctgag gcaactttta ataaaagtac ccttgttgag
      841 tacagtggtg tcagtcacga acccaaacag cacagaactg ccccttatcc acctcaaaat
      901 atttccgttc gtatcgtaaa cttgaacaaa aacaactggg aagaacagag tggcaatttc
      961 ccagaagaat ccttcatgag atcacaagat acaataggaa aagaaaaact cttccatttt
     1021 acagaagaaa cccctgaaat tccctcgggc aacatttctt ccggttggcc tgattttaat
     1081 agcagtgact atgaaactac gtctcagcca tattggtggg acagtgcatc tgcagctcct
     1141 gaaagtgaag atgaatttgt cagcgtactt cccatggaat acgaaaataa cagtacactc
     1201 agtgagacag agaagtcaac atcaggctct ttctcctttt tccctgtgca aatgatattg
     1261 acctggttac cacccaaacc acccactgct tttgatgggt tccatatcca tattgaacga
     1321 gaagagaact ttactgaata tttgatggtg gatgaagaag cacatgaatt tgttgcagaa
     1381 ctgaaggaac ctgggaaata taagttatct gtgacaacct ttagttcctc aggatcttgt
     1441 gaaactcgaa aaagtcagtc agcaaaatca ctcagctttt atatcagtcc ttcaggagag
     1501 tggattgaag aactgaccga gaagccgcag cacgtgagtg tccacgtttt aagctcaacc
     1561 actgccttga tgtcctggac atcttcccaa gagaactaca acagcaccat tgtgtctgtg
     1621 gtgtcgctga cctgccagaa acaaaaggag agccagaggc ttgaaaagca gtactgcact
     1681 caggtgaact caagcaaacc tattattgaa aatctggttc ctggtgccca gtaccaggtt
     1741 gtaatatacc taaggaaagg ccctttgatt ggaccacctt cagatcctgt gacatttgct
     1801 attgttccca caggaataaa ggatttaatg ctctatcctt tgggtcctac ggccgtggtt
     1861 ctgagctgga ccagacctta tttaggcgtg ttcagaaaat acgtggttga aatgttttat
     1921 ttcaaccctg ctacaatgac atcagagtgg accacctact atgaaatagc agcaactgtt
     1981 tccttaactg catccgtgag aatagctaat ctgctgccag catggtacta caacttccgg
     2041 gttaccatgg tgacgtgggg agatccagaa ttgagctgct gtgacagctc taccatcagc
     2101 ttcataacag ccccagtggc tccggaaatc acttctgtgg aatatttcaa cagtctgtta
     2161 tatatcagtt ggacatatgg ggatgataca acggacttgt cccattctag aatgcttcac
     2221 tggatggtgg ttgcagaagg aaaaaagaaa attaaaaaga gtgtaacacg caatgtcatg
     2281 actgcaattc tcagcttgcc tccaggcgac atctataacc tctcagtaac tgcttgtact
     2341 gaaagaggaa gtaatacctc catgctccgc cttgtcaagc tagaaccagc tccacccaaa
     2401 tcactcttcg cagtgaacaa aacccagact tcagtgactt tgctgtgggt ggaagaggga
     2461 gtagctgatt tctttgaagt tttctgtcaa caagttggct ccagtcagaa aaccaaactt
     2521 caggaaccag ttgctgtttc ttcccatgtc gtgaccatct ccagccttct tcctgccact
     2581 gcctacaatt gtagtgtcac cagctttagc catgacagcc ccagtgtccc tacgttcata
     2641 gccgtctcaa caatggttac agagatgaat cccaatgtgg tagtgatctc cgtgctggcc
     2701 atccttagca cacttttaat tggactgttg cttgttaccc tcattattct taggaaaaag
     2761 catctgcaga tggctaggga gtgtggagct ggtacatttg tcaattttgc atccttagag
     2821 agggatggaa agcttccata caactggcgt aggagtatat ttgctttctt aaccctgcta
     2881 ccctcatgtc tttggactga ttatcttttg gcattttata ttaatccttg gagtaaaaat
     2941 ggtttaaaga agaggaaact gacaaacccg gttcaactgg atgactttga tgcctatatt
     3001 aaggatatgg ccaaagactc tgactataaa ttttctcttc agtttgagga gttgaaattg
     3061 attggactgg atatcccaca ctttgctgca gatcttccac tgaatcgatg taaaaaccgt
     3121 tacacaaaca tcctaccata tgacttcagc cgtgtgagat tagtctccat gaatgaagag
     3181 gaaggtgcag actacatcaa tgccaactat attcctggat acaactcacc ccaggagtat
     3241 attgccaccc aggggccact gcctgaaacc agaaatgact tctggaagat ggtcctgcaa
     3301 caaaagtctc agattattgt catgctcact cagtgtaatg agaaaaggag ggtgaaatgt
     3361 gaccattact ggccattcac ggaagaacct atagcctatg gagacatcac tgtggagatg
     3421 atttcagagg aagagcagga cgactgggcc tgtagacact tccggatcaa ctatgctgac
     3481 gagatgcagg atgtgatgca ttttaactac actgcatggc ctgatcatgg tgtgcccaca
     3541 gcaaatgctg cagaaagtat cctgcagttt gtacacatgg tccgacagca agctaccaag
     3601 agcaaaggtc ccatgatcat tcactgcagt gctggcgtgg gacggacagg aacattcatt
     3661 gccctggaca ggctcttgca gcacattcgg gatcatgagt ttgttgacat cttagggctg
     3721 gtgtcagaaa tgaggtcata ccggatgtct atggtacaga cagaggagca gtacattttt
     3781 atccatcagt gtgtgcaact gatgtggatg aagaagaagc agcagttctg catcagtgat
     3841 gtcatatacg agaatgttag caagtcctag ttcagaatcc ggagcagaga ggacatgatg
     3901 tgcgcccatc ctcccttgct tccagattgt tttagtgggc cctgatggtc atttttctaa
     3961 acagaggccc tgctttgtaa tatgtggcca aggagataat ttatctcaca gaagcaccgg
     4021 gaagacttag ccttaaagag cctacagtgt ccttttggac tctttcactt cgggacattt
     4081 aataatggac caaattcaac agaacaccag gaaggtcaag acgctctcca aagggcagga
     4141 agtacagcac ttccgaagag tttagttggc cctttgctgg ttgggctgag ttttttattt
     4201 ttaagtgttt gtttttcagt gcaataattt ttgtgtgtgt gtgattctta tcagaaagtt
     4261 gaattgtttt ctgcctacac cgttcatcag ccccataacc caggaaggaa caggcattgt
     4321 tagcatcaga ttatacctca ttattaaaag gaggcatggc cacacatgaa gaaatggtca
     4381 ttctacttca aagaaattga gccagcacta tctgtactcc aacattaccg gatctggatt
     4441 ggggaggttg gtcagggaag agaggggttc tacccacaga tcaactgtgt aatcttttac
     4501 tattcaagct ataattcagc ttcaaagtag agtagaaaaa aaattgtctt aactgttcta
     4561 gttcttgatg gttttcttcc ttattaacag ttggtgtttc ttccttggcc cttttggact
     4621 aatgttactg tccaagttct ttctcaagaa accacatctg gttcagaaga gtgtcaagtt
     4681 ggactctttg aactctgttg ctgtctgagc aatcgtggtg cctagacttt gcattccttg
     4741 ttctgttgac ctgcatacat gtgagagcta tttctttaag aactatatag gctgtgaaaa
     4801 cgcactttct ttcccccaaa gagctgggaa tttatgaagt tatggcaatg aactgcagca
     4861 tgctgggaca attatttgac tacttttttt tgtaatattg tcaaatgtct ctatggattc
     4921 tgacagagat ttctttttgt tttgttattc ttttggttgt cagtttcatt ttaacgagtg
     4981 taactagtaa cattttattc tttggatttt gtataattac agtacatgat tgtgtattgt
     5041 gacatgaatg ctgtcaaaat gacattgatg gcattgtgaa gcctgttact ttgtgtcact
     5101 tcctgataaa taagaggtga tgacatggat atacaacaga aaacactttg agttgaaagt
     5161 aaacacaagc tggctgcttc cctgtggcaa ctgtggctat tgtgagcctc cgtgtgtgtg
     5221 cttccaggag gctttgctcc tcttcatgca cccgacactg ccccttccct ggcttccctc
     5281 catccctgat catcttcatg aagccacaga tataagatca gcttaatatt ttatcagtga
     5341 ccttagtgtt ggtgaaggat gccaaatgaa cagctctgca ccccaccgtc tcttgtcact
     5401 gaaaaggctt ggccatggca gaccttggca gagtttcttg caagatctgg ccagtgagtc
     5461 tcagtttcac cttgcagggt gaatggcaga ggatgcccat accctgcagg ttaacagctt
     5521 gggctcctca ggggttgtca gttcctactc acctccctgc taccacctcc tcctcttctt
     5581 ctcacccccg actcctttat ttcccttttt ggacatttta ataatgtgtc gcaccattct
     5641 caggaacaaa catggttaat gaaaggtggc aaagggatgt gtgacattct tagattttca
     5701 cacatgggca gtcaggagtg aaatgtgcaa ccttggaaga gctgatacaa tgtatggtaa
     5761 gctgaagtac gtttccaaca ctgctgctca gagcatacag aagttttgca tcctggaaca
     5821 ttgcttccca tgaaacaaag cacttcataa atattttatg tttttcttga gcaccctttc
     5881 aaggtaggga tcaaatagtg ccagatcaat tttcccaaaa ggaaagagga agtatccaga
     5941 gtttagactg aaggcttaga gtcaaaacta caggaaggtc acactgttct ctggaacact
     6001 gttgctcttc agtttgtaga gtttatgatg gaaaaggcat cagtctgtca aaagacttgt
     6061 ttaatcctga taatgacttt ggaattatct ctcctgggtc tcagttaata aactgaagaa
     6121 tgacctaa
//
