LOCUS       NM_002830              11090 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens protein tyrosine phosphatase non-receptor type 4
            (PTPN4), mRNA.
ACCESSION   NM_002830
VERSION     NM_002830.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Zhao,X., Zhu,X., Xiao,C. and Hu,Z.
  TITLE     LncRNA SH3BP5-AS1 promotes hepatocellular carcinoma progression by
            sponging miR-6838-5p and activation of PTPN4
  JOURNAL   Aging (Albany NY) 16 (10), 8511-8523 (2024)
   PUBMED   38761175
  REMARK    GeneRIF: LncRNA SH3BP5-AS1 promotes hepatocellular carcinoma
            progression by sponging miR-6838-5p and activation of PTPN4.
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Liu,Y., Qin,Y. and Zhang,Y.
  TITLE     circRNA-PTPN4 mediated regulation of FOXO3 and ZO-1 expression:
            implications for blood-brain barrier integrity and cognitive
            function in uremic encephalopathy
  JOURNAL   Cell Biol Toxicol 40 (1), 22 (2024)
   PUBMED   38630149
  REMARK    GeneRIF: circRNA-PTPN4 mediated regulation of FOXO3 and ZO-1
            expression: implications for blood-brain barrier integrity and
            cognitive function in uremic encephalopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11090)
  AUTHORS   Chen,C., Wang,Y., Zhao,Q., Li,G.D., Wang,Y.H., Xu,L.C., Huang,H.Z.,
            Song,G., Li,W.T. and He,X.H.
  TITLE     E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and
            Metastasis by Activating STAT3 via Degradation of PTPN4
  JOURNAL   Pancreas 52 (4), e224-e234 (2023)
   PUBMED   37747937
  REMARK    GeneRIF: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer
            Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
REFERENCE   4  (bases 1 to 11090)
  AUTHORS   Gan,J., Liu,S., Zhang,Y., He,L., Bai,L., Liao,R., Zhao,J., Guo,M.,
            Jiang,W., Li,J., Li,Q., Mu,G., Wu,Y., Wang,X., Zhang,X., Zhou,D.,
            Lv,H., Wang,Z., Zhang,Y., Qian,C., Feng,M., Chen,H., Meng,Q. and
            Huang,X.
  TITLE     MicroRNA-375 is a therapeutic target for castration-resistant
            prostate cancer through the PTPN4/STAT3 axis
  JOURNAL   Exp Mol Med 54 (8), 1290-1305 (2022)
   PUBMED   36042375
  REMARK    GeneRIF: MicroRNA-375 is a therapeutic target for
            castration-resistant prostate cancer through the PTPN4/STAT3 axis.
REFERENCE   5  (bases 1 to 11090)
  AUTHORS   Lee,H.S., Yun,H.Y., Lee,E.W., Shin,H.C., Kim,S.J. and Ku,B.
  TITLE     Structural and biochemical analysis of the PTPN4 PDZ domain bound
            to the C-terminal tail of the human papillomavirus E6 oncoprotein
  JOURNAL   J Microbiol 60 (4), 395-401 (2022)
   PUBMED   35089587
  REMARK    GeneRIF: Structural and biochemical analysis of the PTPN4 PDZ
            domain bound to the C-terminal tail of the human papillomavirus E6
            oncoprotein.
REFERENCE   6  (bases 1 to 11090)
  AUTHORS   Gjorloff-Wingren,A., Saxena,M., Han,S., Wang,X., Alonso,A.,
            Renedo,M., Oh,P., Williams,S., Schnitzer,J. and Mustelin,T.
  TITLE     Subcellular localization of intracellular protein tyrosine
            phosphatases in T cells
  JOURNAL   Eur J Immunol 30 (8), 2412-2421 (2000)
   PUBMED   10940933
REFERENCE   7  (bases 1 to 11090)
  AUTHORS   Hironaka,K., Umemori,H., Tezuka,T., Mishina,M. and Yamamoto,T.
  TITLE     The protein-tyrosine phosphatase PTPMEG interacts with glutamate
            receptor delta 2 and epsilon subunits
  JOURNAL   J Biol Chem 275 (21), 16167-16173 (2000)
   PUBMED   10748123
REFERENCE   8  (bases 1 to 11090)
  AUTHORS   Gu,M., Meng,K. and Majerus,P.W.
  TITLE     The effect of overexpression of the protein tyrosine phosphatase
            PTPMEG on cell growth and on colony formation in soft agar in COS-7
            cells
  JOURNAL   Proc Natl Acad Sci U S A 93 (23), 12980-12985 (1996)
   PUBMED   8917530
REFERENCE   9  (bases 1 to 11090)
  AUTHORS   Gu,M. and Majerus,P.W.
  TITLE     The properties of the protein tyrosine phosphatase PTPMEG
  JOURNAL   J Biol Chem 271 (44), 27751-27759 (1996)
   PUBMED   8910369
REFERENCE   10 (bases 1 to 11090)
  AUTHORS   Gu,M.X., York,J.D., Warshawsky,I. and Majerus,P.W.
  TITLE     Identification, cloning, and expression of a cytosolic
            megakaryocyte protein-tyrosine-phosphatase with sequence homology
            to cytoskeletal protein 4.1
  JOURNAL   Proc Natl Acad Sci U S A 88 (13), 5867-5871 (1991)
   PUBMED   1648233
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M68941.1, BC010674.1 and
            AC016691.10.
            
            On Nov 22, 2018 this sequence version replaced NM_002830.3.
            
            Summary: The protein encoded by this gene is a member of the
            protein tyrosine phosphatase (PTP) family. PTPs are known to be
            signaling molecules that regulate a variety of cellular processes
            including cell growth, differentiation, mitotic cycle, and
            oncogenic transformation. This protein contains a C-terminal PTP
            domain and an N-terminal domain homologous to the band 4.1
            superfamily of cytoskeletal-associated proteins. This PTP has been
            shown to interact with glutamate receptor delta 2 and epsilon
            subunits, and is thought to play a role in signalling downstream of
            the glutamate receptors through tyrosine dephosphorylation.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.276116.1,
                                           SRR1660803.170204.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263708.7/ ENSP00000263708.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                M68941.1           292-344
            54-3652             BC010674.1         1-3599
            3653-11090          AC016691.10        20797-28234
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.2"
     gene            1..11090
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /note="protein tyrosine phosphatase non-receptor type 4"
                     /db_xref="GeneID:5775"
                     /db_xref="HGNC:HGNC:9656"
                     /db_xref="MIM:176878"
     exon            1..463
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            464..618
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             481..3261
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /EC_number="3.1.3.48"
                     /note="megakaryocyte phosphatase; PTPase-MEG1; protein
                     tyrosine phosphatase MEG1; megakaryocyte protein-tyrosine
                     phosphatase; protein tyrosine phosphatase, non-receptor
                     type 4 (megakaryocyte)"
                     /codon_start=1
                     /product="tyrosine-protein phosphatase non-receptor type
                     4"
                     /protein_id="NP_002821.1"
                     /db_xref="CCDS:CCDS2129.1"
                     /db_xref="GeneID:5775"
                     /db_xref="HGNC:HGNC:9656"
                     /db_xref="MIM:176878"
                     /translation="MTSRFRLPAGRTYNVRASELARDRQHTEVVCNILLLDNTVQAFK
                     VNKHDQGQVLLDVVFKHLDLTEQDYFGLQLADDSTDNPRWLDPNKPIRKQLKRGSPYS
                     LNFRVKFFVSDPNKLQEEYTRYQYFLQIKQDILTGRLPCPSNTAALLASFAVQSELGD
                     YDQSENLSGYLSDYSFIPNQPQDFEKEIAKLHQQHIGLSPAEAEFNYLNTARTLELYG
                     VEFHYARDQSNNEIMIGVMSGGILIYKNRVRMNTFPWLKIVKISFKCKQFFIQLRKEL
                     HESRETLLGFNMVNYRACKNLWKACVEHHTFFRLDRPLPPQKNFFAHYFTLGSKFRYC
                     GRTEVQSVQYGKEKANKDRVFARSPSKPLARKLMDWEVVSRNSISDDRLETQSLPSRS
                     PPGTPNHRNSTFTQEGTRLRPSSVGHLVDHMVHTSPSEVFVNQRSPSSTQANSIVLES
                     SPSQETPGDGKPPALPPKQSKKNSWNQIHYSHSQQDLESHINETFDIPSSPEKPTPNG
                     GIPHDNLVLIRMKPDENGRFGFNVKGGYDQKMPVIVSRVAPGTPADLCVPRLNEGDQV
                     VLINGRDIAEHTHDQVVLFIKASCERHSGELMLLVRPNAVYDVVEEKLENEPDFQYIP
                     EKAPLDSVHQDDHSLRESMIQLAEGLITGTVLTQFDQLYRKKPGMTMSCAKLPQNISK
                     NRYRDISPYDATRVILKGNEDYINANYINMEIPSSSIINQYIACQGPLPHTCTDFWQM
                     TWEQGSSMVVMLTTQVERGRVKCHQYWPEPTGSSSYGCYQVTCHSEEGNTAYIFRKMT
                     LFNQEKNESRPLTQIQYIAWPDHGVPDDSSDFLDFVCHVRNKRAGKEEPVVVHCSAGI
                     GRTGVLITMETAMCLIECNQPVYPLDIVRTMRDQRAMMIQTPSQYRFVCEAILKVYEE
                     GFVKPLTTSTNK"
     exon            619..726
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            727..769
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            770..848
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            849..893
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            894..946
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1067
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1155
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1244
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1308
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1481
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1550
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1676
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1835
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1995
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2079
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2136
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2293
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2460
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2551
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2613
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2760
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2889
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..3038
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3174
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3175..11090
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9977..9982
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /note="hexamer: AATAAA"
     polyA_site      10001
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /note="major polyA site"
     regulatory      11064..11069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /note="hexamer: AATAAA"
     polyA_site      11090
                     /gene="PTPN4"
                     /gene_synonym="MEG; PTPMEG; PTPMEG1"
                     /note="major polyA site"
ORIGIN      
        1 aggattaccc gccagctcac gccgcgcagt gcgcttttcc gctcctcgcg ccccaccacc
       61 aacattgttc tctcaggact cctgggtccc aggggccgga attgggcctg agcgggagag
      121 gaaagagact tggctttggc cgcggggtcg gaggattggg gccaggcccc ctcccccacg
      181 cacttttggg ggtgtggatt atctcatccc tgcagggagg taggagaggt cgccggctgc
      241 ccgcctccct gccacctccc cagcggcgcc ggcccgcggc tgcccagcag catgaggtgg
      301 tgctggcggc tccgggtcgt ggcgcgaccg ctgcggcggc ggctgctcgg ggggcgctga
      361 ggtagccccc cggagcggca cggaggacgc gcttctcctc tgcgcgccgg ggcctcgagg
      421 ctttttttct ccagccgaga ggacgcggct gtgatatacg aagactttgt gtggacagta
      481 atgacctcac gtttccgatt gcctgctggc agaacctaca atgtacgagc atcagagttg
      541 gcccgagaca gacagcatac tgaagtggtt tgcaacatcc ttcttctgga taacactgta
      601 caagctttca aagtcaataa acatgatcag gggcaagtct tgttggatgt cgtcttcaag
      661 catctagatt tgactgagca ggactatttt ggtttacagt tggctgatga ttccacagat
      721 aacccaaggt ggctggatcc aaacaaacca ataaggaagc agctaaagag aggatctcct
      781 tacagtttga actttagagt caaatttttt gtaagtgacc ccaacaagtt acaagaagaa
      841 tatacaaggt accagtattt tttgcaaatt aaacaagaca ttcttactgg aagattaccc
      901 tgtccttcta atactgctgc ccttttagct tcatttgctg ttcagtctga acttggagac
      961 tacgatcagt cagagaactt gtcaggctac ctctcagatt attctttcat tcctaatcaa
     1021 cctcaagatt ttgaaaaaga aattgcaaaa ttacatcagc aacacatagg cttatctcct
     1081 gcagaagcag aatttaatta cctaaacaca gcacgtacct tagaactcta tggagttgaa
     1141 ttccactatg caagggatca gagtaacaat gaaattatga ttggagtgat gtcaggagga
     1201 attctgattt ataagaacag ggtacgaatg aatacctttc catggttgaa gattgtaaaa
     1261 atttctttta agtgcaaaca gttttttatt caacttagaa aagaattgca tgaatctaga
     1321 gaaacattat tgggatttaa tatggtgaat tacagagcat gtaaaaattt gtggaaagca
     1381 tgtgtagaac atcacacatt cttccgtttg gacagaccac ttccacctca aaagaatttt
     1441 tttgcacatt attttacatt aggttcaaaa ttccggtact gtgggagaac tgaagtccaa
     1501 tcagttcagt atggcaaaga aaaggcaaat aaagacaggg tatttgcaag atccccaagt
     1561 aagcccttgg cacggaaatt aatggattgg gaagtagtaa gcagaaattc aatatctgat
     1621 gacaggttag aaacacaaag tcttccatca cgatctccac cgggaactcc taatcatcga
     1681 aattctacat tcacgcagga aggaacccgg ttacgaccat cttcagttgg tcatttggta
     1741 gaccatatgg ttcatacttc cccaagcgaa gtgtttgtaa atcagagatc tccgtcatca
     1801 acacaagcta atagcattgt tctggaatca tcaccatcac aagagacccc tggagatggg
     1861 aagcctccag ctttaccacc caaacagtca aagaaaaaca gttggaacca aattcattat
     1921 tcacattcgc aacaagatct agaaagtcat attaatgaaa catttgatat tccatcttct
     1981 cctgaaaaac ccactcctaa tggtggtatt ccacatgata atcttgtcct aatcagaatg
     2041 aaacctgatg aaaatgggag gtttggattc aatgtaaagg gaggatatga tcagaagatg
     2101 cctgtgattg tgtctcgagt agcaccagga acacctgctg acctctgtgt ccctagactg
     2161 aatgaagggg accaagttgt actgatcaat ggtcgggaca ttgcagaaca cactcatgat
     2221 caggttgtgc tgtttattaa agctagttgt gagagacatt ctggggaact catgcttcta
     2281 gttcgaccta atgctgtata tgatgtagtg gaagaaaagc tagaaaatga gccagatttc
     2341 cagtatattc ctgagaaagc cccactagat agtgtgcatc aggatgacca ttccctgcgg
     2401 gagtcaatga tccagctagc tgaggggctt atcactggaa cagtcctgac acagtttgat
     2461 caactgtatc ggaaaaaacc tggaatgaca atgtcctgtg ccaaattacc tcagaatatt
     2521 tccaaaaata gatacagaga tatttcgcct tatgatgcca cacgggtcat tttaaaaggt
     2581 aatgaagact acatcaatgc gaactatata aatatggaaa ttccttcttc cagcattata
     2641 aatcagtaca ttgcttgtca agggccatta ccacacactt gtacagattt ttggcagatg
     2701 acttgggaac aaggctcctc tatggttgta atgttgacca cacaagttga acgtggcaga
     2761 gttaaatgtc accaatattg gccagaaccc acaggcagtt catcttatgg atgctaccaa
     2821 gttacctgcc actctgaaga aggaaacact gcctatatct tcaggaagat gaccctattt
     2881 aaccaagaga aaaatgaaag tcgtccactc actcagatcc agtacatagc ctggcctgac
     2941 catggagtcc ctgatgattc gagtgacttt ctagattttg tttgtcatgt acgaaacaag
     3001 agggctggca aggaagaacc cgttgttgtc cattgcagtg ctggaatcgg aagaactggg
     3061 gttcttatta ctatggaaac agccatgtgt ctcattgaat gcaatcagcc agtttatcca
     3121 ctagatattg taagaacaat gagagatcag cgagccatga tgatccaaac acctagtcaa
     3181 tacagatttg tatgtgaagc tattttgaaa gtttatgaag aaggctttgt taaaccctta
     3241 acaacatcaa caaataaata agaaagcaaa aagatctggg atatgtgttg gaaaactgct
     3301 ttcccttatg ttcactgtgc cataatgctg ctcgcaggaa atggcatttt acaaaaaaaa
     3361 aatgaagaac tcaaaaaaac tttgaaaact tcagcactgt tgcactttat gttttaaaaa
     3421 atgtcactct ttcaaaatct ataactcatg tatttgaaga ctgtttcatg ctttgctccg
     3481 aacaaatagt aaataactga gtatgttcag ggtaatttat gaaattttgt ggtggtgcca
     3541 tgcaatcccc ttttggtaga attgccacaa acaaggctca aaattctcat catctctgtt
     3601 atacacctgt atcatgaaag caaaaagaag taaacatcag gagtcagctc tggtcttttg
     3661 cagtggtttg cgtacttact atactgatta ccagatttta tttttaaaat tttagcaata
     3721 tcgttcttaa tattagccaa tacactgcct atggatgcag cacatttttc cctgcacacc
     3781 ccctgtagag acctgcctgc tgctcagaag aagaaagatg gaatttgctg ttcccaggaa
     3841 atgctgcaca ttgtccattt accagcatct tatagaaaat ataaatatga atctacaaat
     3901 tctcttggat ttaataatgt aacttatatt tatcataagg ttggcttatt ccaaatcatg
     3961 tgatttcaca tacttgatgt aaaggaatgc atgcattgtg tcttaacccc ttaagtgcct
     4021 tgagacgtcc ttgtatgtct aacgaaaagg cactcatttg accccactag gaggtatata
     4081 tgtatattgt acattcgtat atttttatgc attttgaata agttcacatt ttaaaaaaca
     4141 gctatattga gtgttataca gtcaaagaag ggtaaggaaa ttctgtttct tactcagtac
     4201 tcgtgatttg ctataagaag caaagataat ttgtgttcta ctgagtgcac actgctgcta
     4261 tggaattgtt caaaatctgc acattccttt acttgaggga ctgaggggtt acggtttagc
     4321 agctcaactt taaaagatgc ttttatgctt caaatttcat atttatttca tccctaactt
     4381 taggaagaca tctaacatgc agattaatgt taaacatatt gataatctaa gctaaagtta
     4441 gagcaaataa ttgtgattta ttcatgtcca taactaaaat attattcttt agcactcatt
     4501 tcagttaata tcaagctttg ttcacactga tatattgatt gggattcttc aaacgaagaa
     4561 tgaaacatta ttgacttatt tctaagtgta ataactcaat aactaaattg agaattttct
     4621 tgtttttttg tgtacaatta aattattcca ttttatatat acttattaag ttataagcat
     4681 gttaataagg agacttttga ctgtccagcc taatggagga tctgattccc attaaggatg
     4741 tgggaattcc cgtattttcc tcttcaggtt tttttgtttt taagtctaca gagagataga
     4801 accttaacat tgcccagttt gataaaagtg aaaatttgct cacctataca aatgttattt
     4861 tacacaatta ttttatgtgg attggggttt taaaagattc atttcctagc agtttctgaa
     4921 tatttctgta agatcttgat aaagtttaga gtcaaatggc agatattttg gaaagccatt
     4981 tagtatgagg ggaagagtac acattttaaa tctttggtat aatctaaacg atgtcatttc
     5041 agacattcaa aactcataat aataaatttc aggaaatcaa gctcctaaac attaatagcc
     5101 aagataccaa ataaattatc atattaaaat atttaagttt atttaaagag agaagtaaac
     5161 ataaacttat gtcaaagaga aattctctta atctatttcc tgacttcaca gtcaaaacag
     5221 tataagatag tgaagtaact tataatctta tttggtcatt ctgttctata aacagacaaa
     5281 tataatttag gctgctatat gacatgataa attaaaaact gagaaagtaa aattatgtcg
     5341 gggtctacac attaaatgac tgttttggca gtttttcacc tgaatttaaa aataaaattt
     5401 gtctgttcta tctgtatgga cagaatatga caaccataga ccaaaagata tcattgtata
     5461 gatgtaaaga gacaaaagtc atggttggca tgttctttaa tttagggcct tgtttatact
     5521 tgtgagttta ataatttctt ggcaatgagc actgctgcct ttttaaatca tcaatgcctg
     5581 tctttaaatg ccaatcatta gaagtttaga attacattta aatttctcat atatgaattt
     5641 ttcatctggg aactacccac tgtcgtcatt tttatgtgca gaaaagatcc tttttgaaaa
     5701 ccatatttat tgggatctta tttaaattta aattatcctt tacaacttct taaaatgaat
     5761 atgctaatag ataagacttt atccattaca tctaagaact ttttaacctg tatatcaatc
     5821 taaataggtt tctagaaata atcctagctt tcatacacca tgtagttcta gactccgtcc
     5881 atatcagtgt aacattgtgt gataaaatgc caaatgtctt gtatctaagc tacgctccca
     5941 ctcttccagc cacccctacc tcccaccttg acacccttaa gaaagcaatg ctgactttta
     6001 ttctgacaag tggaaatcaa tagtggttaa tcaagtggat tttgtaaatg ctaagaagtg
     6061 tttgtgaaaa ccttcccttt ctgagttagg attgtttttt cctgttcatt tcaggtgttt
     6121 tattgagcat ctgatttgtg tcagcaccat gtaaatatga tgaggagtat ttggaataga
     6181 ctttacattc accagaaaat tgatagtatt tgttaaacca atgcatccat tcaaaataga
     6241 ggcagagtaa acagcctaag aaatgatttc ctttctacag tctgctagga gaaagaggtg
     6301 agaggggagt ggttgatgat tttaatcaag agtaaaggga acatttatta catgaaatct
     6361 gacttcagtt gtgcaaaggt atgttaagac attaagacaa ttgctggaag gtttcaaata
     6421 tgtgtacaca cacatagagc tacttttgtg tgtttattta tatgtatatt tcacaaaggc
     6481 taatgcccac agaggaaaat gattatttta acttctgggt tatcatctgc tggcagggtc
     6541 tgatcaggtc tagagtattt aaggaaaagt ctcctgtctt ctatctccac tagaagtact
     6601 gcagcttgag agctgaaagg aacttgaaaa atgttatcca gtcttcactt agcccatttt
     6661 tatcggcatt aggaaatagg gttcagcaat ggttattgat ttagcagtct ggtcttatat
     6721 gatctgcttt ctaattatct atctagtatt cctatattat actggaactt caataaatta
     6781 taacctatgt gaatatcata aattcttgtg aatttttgtc tatttccagt agagaagcat
     6841 actcagccaa tgttttcact taataactat gacaacctta aaggatgtca cttaagttag
     6901 aaaataagca ggcttaagaa gtgattctaa ttaggacccc cactcagttt gctagtcatg
     6961 cctgcctgac agaacataag cttttgtaaa gtatttagac tgataacaca tgcagtatga
     7021 aaacattata gactgcagtt agaagactaa ttttttaaaa gcactcaaaa catagaacta
     7081 agatttagaa tatatgtcca tttataatga aataatttgt gttcataatg tgaacatgta
     7141 agataagtaa ataaagcatt acttgtttta taatatcagg aagagagaag gaaacattag
     7201 tttataacaa tgtgctattt aatatctgta ttttgaacat ttaaaatact ctgatggtaa
     7261 ggttgccaaa gtaattttga attcataaaa attgtttata ccacaataat gtgggatatg
     7321 tttacaggct cttttatttt gtggtagtta ctttatagtt cagatgaatt tgcatttcag
     7381 tcacttatag taaataaagt atattaaata tttgttggaa taaaaatctc aatgtaatag
     7441 gacatagata aattatgcaa cagcctgctt tgatgaaagc tggtgcatta tgtgagctgc
     7501 cacagttaat actttgtatt aacattgctt acttccccca tttgcttcac ctatctgact
     7561 acaattgctg gaatcattct ttttgtggtg cttcctccaa gtgaaataac tatgttatta
     7621 aaacctactg gattaatttt atcaaatatc agacttactg atctcttggc agagatttat
     7681 tggtgtctca gtgtgatcac aatacaagtt gtagcaacca ttttataaaa ctcttagaga
     7741 attatgtaga taaaatggaa attacataat cctaaaacat ttttgagggc taattgataa
     7801 taatttcagc aattttatta agaacccagt aaattaatcc ctaatttgta tgttttttgt
     7861 tcaacttacc gtgtctgcaa ccattatata gacttcattg atactcagca attactacag
     7921 tgtgtcttac aatgttataa aaaacaaaca gcaatctctg tttctgccag aagttagata
     7981 tttttttata gtcctgatga atttgttgtg agtagacacc acatagttat ttctgtttgc
     8041 atataaattt gctgctattt atttgtatgt ttttctactg tagtatgcta ttgatttctg
     8101 aagttataat cataattttt attttagtaa tccaagctaa atgggcaacc agaagtaata
     8161 tctgctaggg tgcaatccat cagcattggt tcagtgcata tacataagta aaccagttta
     8221 aaaaaatttt actaagcaaa aggtagtagc aatattgcta gttaaagatt accattttag
     8281 gggaaagttc aaaggtttaa cttcctgaaa ccttttaaac ttttcagtgc aagaatgaaa
     8341 atgtttgaaa gccttgttaa gctcataaaa gtttcattta aacactgtag agtttcctac
     8401 ctttgttgtg taaaggatat agaaaatatt tttctatgga cagaaggttg aaactgttaa
     8461 cttctttaat ttcattgttt aaaggagata aaatgttatt tgtgtgtctt tcatgctgta
     8521 aagaaaatga atgtatcttt caaatgtcat tagaattatg gcttgattct aaggatactc
     8581 tatttgtttt aaagtctaca cgggattgaa aagaaaggga agagtattac acttcattgt
     8641 acaaaatgta attttagaag aaaaacattt tcattataaa caagagcaat cattgaagca
     8701 ttttcagata taatgtacat tctttttcat tttgctttgc ctctagaata gaaacttttc
     8761 agaaaatttg cacatactgt ttgtaagtat aaaataattg aattctagac ttttggagtg
     8821 tggaagggtg tttgtgtgtg aaggtaataa atgtgttgct gcatgtgttt accatagaga
     8881 acgttgccag gctttgtcaa atgaggacat tgttgtgagg gtgtgacttt tgtgaagcca
     8941 gaaaactgtg cctaagattc attacagtga gtatgtgcag aggcccctga cccagtaaat
     9001 gctttttgcc tttttattcc caatgtcttg gacagttgag acaacttggc ccaactctga
     9061 attgtagcag tgatgtctta tatgcttacg agtttatatt aattcagctg aacaagaact
     9121 acatcttaaa ccacctttgt aggtatgatt aatacgtgca acagctgctt taaaaggtgg
     9181 tatttattat tcatcactca tgaaacttga ctcagtgagg acttcattat ttttatttct
     9241 ctttccacgt ctccaacttt ttaaaaatca ctaatttaac tctttgttaa gtactcaagt
     9301 acaaagtgat ctcttctttc atgaactttg ctataactgt tgaaaggatt ggtgtcaggc
     9361 tctctcaacc ctgggagttt ccaaaattta gcaaatatta cttgtgataa gcttaaatgc
     9421 cacatcgcta agtacaatta ttaccaggaa tccgtcaagg gggagatagc cccgctcaca
     9481 cccttagacc caaacaggtg ctggttcatg ctttctccac aaattggcag ttgggtactt
     9541 ccccactgtc atcacagttg agctcatttt aacatatatt caaagaagct ttttagtttc
     9601 tactcctgaa ttgtagtgtt caagttattt gccttcctgg gtaagaaaat tttaacccaa
     9661 gtctgggatt ctctggcacc tgtcttttcc catcttttaa tgagttggca agattaggtc
     9721 aaatttcaat gctaaatttg attttttgaa ttttaaatag ttgcacttta tttcatgctg
     9781 accaccaact taatttatac attttaaaat aaaattatcc tagaagtgtt gtaaacgtgg
     9841 gaaaatttta attagtgttg gaggtcatta gattggaccc aggtttaaat ataatgatct
     9901 cttcacagct tatttgatgt ttaagagcaa cttgtgtgat cttagctttg caggttttct
     9961 gtaatttatg tagcacaata aaggataacc agttgactgt gttactggac tctgagttct
    10021 cacagctagt ttcatctaag cttggtttat tatcatttct gctttggatt tttttgtgca
    10081 tcacatgaat acttagaaat ccatttgttt tcagtgtagt acctagggtg aagtagatgc
    10141 tgcacaagta agtttaaggg aataaaagtc ccgacacttt atatacatgt tgaggggcac
    10201 gatttaagag actgaaacag tttacattaa actgttttta ttttctgcca gtagactcta
    10261 tctgcttaaa aaaataaaaa ttgttcaacc cagtgttctc cagcatcagg acattacagt
    10321 tgtaatctat gttgtaatct tttcaaataa gaaaagctac tccttattct ctacagtgta
    10381 ggcttaattt tagaaccgat aatttactat atctgcatta ttgatatttt taagtagtag
    10441 ttttaaaaat aattatttct atgtggaggg tgttttaatt tgggattttt tttttcttgt
    10501 aacaggtgct atttgtaaat atgaagggga aaagtcactt agttaaaagt ctagcttatg
    10561 tcataattaa gatacaatta ttcatttcat gtttgattct attaaactag tggcaaaaca
    10621 gaattggtcc cttagttttt tagattacct ttccccctat atcacaaaaa tatctctttc
    10681 catatgatct cataattgag gcaaagaagc taagggttta tttaaaatgt gtataagctt
    10741 gaatttggtc aacactgcat aatttgaaat cactctgcat ttgtcactgc agcttactgt
    10801 atgcttgaaa ggccttgtgt gtttgtctta attttagtga aaaaattaga atttctgcca
    10861 ttcatgtaca aaaaaattac aactacagca aacaagataa aaatgctggt ttgcattaat
    10921 actgttttag tcttaagagc aatttatatt atgtgaaatg ctgttacaca tattttgttg
    10981 gccatatttc attttgagaa acagttgttc aggtacaaac atgacaaaca actctagcta
    11041 tgactcttaa tgttcaattg caaaataaag atgtgcttta gtaatctaaa
//
