LOCUS       NM_178000               2640 bp    mRNA    linear   PRI 20-AUG-2024
DEFINITION  Homo sapiens protein phosphatase 2 phosphatase activator (PTPA),
            transcript variant 2, mRNA.
ACCESSION   NM_178000
VERSION     NM_178000.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Crespo,J.R., Martin-Martin,N., Garcia-Longarte,S.,
            Corres-Mendizabal,J., Carlevaris,O., Astobiza,I., Zabala-Letona,A.,
            Guiu,M., Azkargorta,M., Gonzalez-Lopez,M., Macias-Camara,N.,
            Doan,P., Elortza,F., Mendizabal,I., Westermack,J., Gomis,R.R.,
            Ercilla,A. and Carracedo,A.
  TITLE     The PP2A regulator IER5L supports prostate cancer progression
  JOURNAL   Cell Death Dis 15 (7), 514 (2024)
   PUBMED   39025841
  REMARK    GeneRIF: The PP2A regulator IER5L supports prostate cancer
            progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Wasserman,J.S., Faezov,B., Patel,K.R., Kurimchak,A.M.,
            Palacio,S.M., Glass,D.J., Fowle,H., McEwan,B.C., Xu,Q., Zhao,Z.,
            Cressey,L., Johnson,N., Duncan,J.S., Kettenbach,A.N., Dunbrack,R.L.
            Jr. and Grana,X.
  TITLE     FAM122A ensures cell cycle interphase progression and checkpoint
            control by inhibiting B55alpha/PP2A through helical motifs
  JOURNAL   Nat Commun 15 (1), 5776 (2024)
   PUBMED   38982062
  REMARK    GeneRIF: FAM122A ensures cell cycle interphase progression and
            checkpoint control by inhibiting B55alpha/PP2A through helical
            motifs.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2640)
  AUTHORS   Ando,S., Tanaka,K., Matsumoto,M., Oyama,Y., Tomabechi,Y.,
            Yamagata,A., Shirouzu,M., Nakagawa,R., Okimoto,N., Taiji,M.,
            Sato,K. and Ohama,T.
  TITLE     The luciferase-based in vivo protein-protein interaction assay
            revealed that CHK1 promotes PP2A and PME-1 interaction
  JOURNAL   J Biol Chem 300 (5), 107277 (2024)
   PUBMED   38588804
  REMARK    GeneRIF: The luciferase-based in vivo protein-protein interaction
            assay revealed that CHK1 promotes PP2A and PME-1 interaction.
REFERENCE   4  (bases 1 to 2640)
  AUTHORS   Wachter,F., Nowak,R.P., Ficarro,S., Marto,J. and Fischer,E.S.
  TITLE     Structural characterization of methylation-independent PP2A
            assembly guides alphafold2Multimer prediction of family-wide PP2A
            complexes
  JOURNAL   J Biol Chem 300 (5), 107268 (2024)
   PUBMED   38582449
  REMARK    GeneRIF: Structural characterization of methylation-independent
            PP2A assembly guides alphafold2Multimer prediction of family-wide
            PP2A complexes.
REFERENCE   5  (bases 1 to 2640)
  AUTHORS   Palanivel,C., Madduri,L.S.V., Hein,A.L., Jenkins,C.B., Graff,B.T.,
            Camero,A.L., Zhou,S., Enke,C.A., Ouellette,M.M. and Yan,Y.
  TITLE     PR55alpha-controlled protein phosphatase 2A inhibits p16 expression
            and blocks cellular senescence induction by gamma-irradiation
  JOURNAL   Aging (Albany NY) 16 (5), 4116-4137 (2024)
   PUBMED   38441530
  REMARK    GeneRIF: PR55alpha-controlled protein phosphatase 2A inhibits p16
            expression and blocks cellular senescence induction by
            gamma-irradiation.
REFERENCE   6  (bases 1 to 2640)
  AUTHORS   Okamoto,K., Kamibayashi,C., Serrano,M., Prives,C., Mumby,M.C. and
            Beach,D.
  TITLE     p53-dependent association between cyclin G and the B' subunit of
            protein phosphatase 2A
  JOURNAL   Mol Cell Biol 16 (11), 6593-6602 (1996)
   PUBMED   8887688
REFERENCE   7  (bases 1 to 2640)
  AUTHORS   Van Hoof,C., Aly,M.S., Garcia,A., Cayla,X., Cassiman,J.J.,
            Merlevede,W. and Goris,J.
  TITLE     Structure and chromosomal localization of the human gene of the
            phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase
            2A
  JOURNAL   Genomics 28 (2), 261-272 (1995)
   PUBMED   8530035
REFERENCE   8  (bases 1 to 2640)
  AUTHORS   Turowski,P., Fernandez,A., Favre,B., Lamb,N.J. and Hemmings,B.A.
  TITLE     Differential methylation and altered conformation of cytoplasmic
            and nuclear forms of protein phosphatase 2A during cell cycle
            progression
  JOURNAL   J Cell Biol 129 (2), 397-410 (1995)
   PUBMED   7721943
REFERENCE   9  (bases 1 to 2640)
  AUTHORS   Cayla,X., Van Hoof,C., Bosch,M., Waelkens,E., Vandekerckhove,J.,
            Peeters,B., Merlevede,W. and Goris,J.
  TITLE     Molecular cloning, expression, and characterization of PTPA, a
            protein that activates the tyrosyl phosphatase activity of protein
            phosphatase 2A
  JOURNAL   J Biol Chem 269 (22), 15668-15675 (1994)
   PUBMED   8195217
REFERENCE   10 (bases 1 to 2640)
  AUTHORS   Jakes,S., Mellgren,R.L. and Schlender,K.K.
  TITLE     Isolation and characterization of an inhibitor-sensitive and a
            polycation-stimulated protein phosphatase from rat liver nuclei
  JOURNAL   Biochim Biophys Acta 888 (1), 135-142 (1986)
   PUBMED   3017441
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL158151.16.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_178000.2.
            
            Summary: Protein phosphatase 2A is one of the four major Ser/Thr
            phosphatases and is implicated in the negative control of cell
            growth and division. Protein phosphatase 2A holoenzymes are
            heterotrimeric proteins composed of a structural subunit A, a
            catalytic subunit C, and a regulatory subunit B. The regulatory
            subunit is encoded by a diverse set of genes that have been grouped
            into the B/PR55, B'/PR61, and B''/PR72 families. These different
            regulatory subunits confer distinct enzymatic specificities and
            intracellular localizations to the holozenzyme. The product of this
            gene belongs to the B' family. This gene encodes a specific
            phosphotyrosyl phosphatase activator of the dimeric form of protein
            phosphatase 2A. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) lacks one of the coding exons
            compared to transcript variant 1. It maintains the reading frame
            and encodes a protein (isoform b), also known as isoform alpha,
            that is missing an internal segment of the protein compared to
            isoform a. Variants 2 and 3 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.648590.1,
                                           SRR3476690.12961.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393370.7/ ENSP00000377036.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-217               AL158151.16        18896-19112
            218-315             AL158151.16        27994-28091
            316-402             AL158151.16        30533-30619
            403-528             AL158151.16        36466-36591
            529-646             AL158151.16        39003-39120
            647-746             AL158151.16        42276-42375
            747-871             AL158151.16        43952-44076
            872-972             AL158151.16        45073-45173
            973-1080            AL158151.16        49926-50033
            1081-2640           AL158151.16        54868-56427
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..2640
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /note="protein phosphatase 2 phosphatase activator"
                     /db_xref="GeneID:5524"
                     /db_xref="HGNC:HGNC:9308"
                     /db_xref="MIM:600756"
     exon            1..217
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..1158
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /EC_number="5.2.1.8"
                     /note="isoform b is encoded by transcript variant 2;
                     protein phosphatase 2A, regulatory subunit B' (PR 53);
                     PP2A phosphatase activator; phosphotyrosyl phosphatase
                     activator; serine/threonine-protein phosphatase 2A
                     regulatory subunit B'; protein phosphatase 2A regulatory
                     subunit 4; protein phosphatase 2 regulatory subunit 4;
                     protein phosphatase 2A activator, regulatory subunit 4"
                     /codon_start=1
                     /product="serine/threonine-protein phosphatase 2A
                     activator isoform b"
                     /protein_id="NP_821067.1"
                     /db_xref="CCDS:CCDS6920.1"
                     /db_xref="GeneID:5524"
                     /db_xref="HGNC:HGNC:9308"
                     /db_xref="MIM:600756"
                     /translation="MAEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQA
                     YADYIGFILTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGN
                     KAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFA
                     AFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFL
                     PFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLW
                     NISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG"
     exon            218..315
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            316..402
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            403..528
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            529..646
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            647..746
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            747..871
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            872..972
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1080
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..2640
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2614..2619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /note="hexamer: ATTAAA"
     polyA_site      2640
                     /gene="PTPA"
                     /gene_synonym="PARK25; PP2A; PPP2R4; PR53"
                     /note="major polyA site"
ORIGIN      
        1 accgacatgg cggccgtctt cgctgtggtg actttaactc tcggttttcg gttatagccg
       61 gccggcgctc acttgtcttc aggaagctcg gagcctttgg tggagccggg gagaggaagg
      121 gtgggtgcaa gagtgaaagg cgagagggga ctgcaagcat ccgggtcggc tcctggccgg
      181 agcaagatgg ctgagggcga gcggcagccg ccgccagatt cttcagagga ggcccctcca
      241 gccactcaga acttcatcat tccaaaaaag gagatccaca cagttccaga catgggcaaa
      301 tggaagcgtt ctcaggcata cgctgactac atcggattca tccttaccct caacgaaggt
      361 gtgaagggga agaagctgac cttcgagtac agagtctccg aggccattga gaaactagtc
      421 gctcttctca acacgctgga caggtggatt gatgagactc ctccagtgga ccagccctct
      481 cggtttggga ataaggcata caggacctgg tatgccaaac ttgatgagga agcagaaaac
      541 ttggtggcca cagtggtccc tacccatctg gcagctgctg tgcctgaggt ggctgtttac
      601 ctaaaggagt cagtggggaa ctccacgcgc attgactacg gcacagggca tgaggcagcc
      661 ttcgctgctt tcctctgctg tctctgcaag attggggtgc tccgggtgga tgaccaaata
      721 gctattgtct tcaaggtgtt caatcggtac cttgaggtta tgcggaaact ccagaaaaca
      781 tacaggatgg agccagccgg cagccaggga gtgtggggtc tggatgactt ccagtttctg
      841 cccttcatct ggggcagttc gcagctgata gaccacccat acctggagcc cagacacttt
      901 gtggatgaga aggccgtgaa tgagaaccac aaggactaca tgttcctgga gtgtatcctg
      961 tttattaccg agatgaagac tggcccattt gcagagcact ccaaccagct gtggaacatc
     1021 agcgccgtcc cttcctggtc caaagtgaac cagggtctca tccgcatgta taaggccgag
     1081 tgcctggaga agttccctgt gatccagcac ttcaagttcg ggagcctgct gcccatccat
     1141 cctgtcacgt cgggctagga ggggccaagc cgaagagcca cccaggccac agttcctgtg
     1201 cctgccttcc ccaccccagc agtggcccct ccccatcccc tccctctgtt cgtcccgttt
     1261 gatgagaggc tgtttactgg ggtggggtgg cgagatgggc ttgagggggc tcagagcata
     1321 aggcttcagg gcccaagttg ggagaagtga ccaaagtgta gccagttttc tgagttcccg
     1381 tgtgctagac tggccagaag agagggtctg gggcctggtc actcggccac tctctcctgt
     1441 ttctggcctc ttctcccttc actcccgtcc agtctggttt tgagagcagg ggctgttctg
     1501 cagcaccgca gggaagggag gagagatacc tgctgcttcc attgcttttc ccttcctgga
     1561 gtcgatgcct ttctaagggt tggagctgct ccttgcaggg gcgggtcagt ttcccaggcc
     1621 atgccggggt ggccatctat ggtagggctg gaagctgagg ctggccgcca gctgtgggct
     1681 ggggtggggt gggtggggtc gggtggtgga gaggccttag ctgtcctggc tggtgcccct
     1741 cccaggctcc ttttcaccct gccccctggg cctgaggccc cctgtgtcca agcctccccc
     1801 tggctcttca gttctctagc ccttggctct gctgggtttc ctgactgtag ccacatctct
     1861 cccgctccct aagggtaacc tagccaatgg aagctgccct ttgggtaggt gctgggctcc
     1921 tgggagggcc cagatgatgg ggtgaggcat gtctttccag aactttccct ggcagggagg
     1981 ggatggcaga aactcaggga ggggcttggg gcccattgta tctggagagc ctggattcct
     2041 cttggcagtc ttaggcccgg ccacttctgc tacctttgcg ctgctgtgag cctcaccctg
     2101 ggcccctggg ccctgcttct ctgctcccct gggtgatggg tgggcccaga aggtggcagt
     2161 cccacacctt gtcctcccac ctccctgaac tgtccattgc ttttataggg tgaggtaaga
     2221 gacagcctcc caagcccagg ctttggcact cagaatgggc ccagtggggg ctgggcaggc
     2281 ccattgaggg ccaccgccga ggtttctcct agggctgttc ctgggcctgg ctcttacagg
     2341 ctcgtccccc aggcctgccc ttctccactg ccccctcctg tgtctgggtc cacacaccct
     2401 tcaggaaggg ggagcactga gaagcacagc acaggggctc agcctgggat ccggtgatgg
     2461 tctgggcaga ggctgggtca ggagtcccaa aggtcagtga cagtttctca gaagaggccc
     2521 agcgtccacc tctctcccag ggccagacag cccttcctgg ctcccccatc cccctatggg
     2581 ctcccagccc cttgcaccct cattgctgtt cagattaaag cctctgtttt gcacctgtca
//
