LOCUS       NM_005975               2953 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens protein tyrosine kinase 6 (PTK6), transcript variant
            1, mRNA.
ACCESSION   NM_005975
VERSION     NM_005975.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Li,Y., Fan,A., Zhang,Y., Meng,W., Pan,W., Wu,F., Ma,Z. and Chen,W.
  TITLE     Circular RNA hsa_circ_0001610 promotes prostate cancer progression
            by sponging miR-1324 and upregulating PTK6
  JOURNAL   Gene 930, 148818 (2024)
   PUBMED   39098513
  REMARK    GeneRIF: Circular RNA hsa_circ_0001610 promotes prostate cancer
            progression by sponging miR-1324 and upregulating PTK6.
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Mandapati,A., Ning,Z., Baharani,A. and Lukong,K.E.
  TITLE     BRK confers tamoxifen-resistance in breast cancer via regulation of
            tyrosine phosphorylation of CDK1
  JOURNAL   Cell Signal 108, 110723 (2023)
   PUBMED   37216999
  REMARK    GeneRIF: BRK confers tamoxifen-resistance in breast cancer via
            regulation of tyrosine phosphorylation of CDK1.
REFERENCE   3  (bases 1 to 2953)
  AUTHORS   Kan,Y., Paung,Y., Kim,Y., Seeliger,M.A. and Miller,W.T.
  TITLE     Biochemical Studies of Systemic Lupus Erythematosus-Associated
            Mutations in Nonreceptor Tyrosine Kinases Ack1 and Brk
  JOURNAL   Biochemistry 62 (6), 1124-1137 (2023)
   PUBMED   36854171
  REMARK    GeneRIF: Biochemical Studies of Systemic Lupus
            Erythematosus-Associated Mutations in Nonreceptor Tyrosine Kinases
            Ack1 and Brk.
REFERENCE   4  (bases 1 to 2953)
  AUTHORS   Liu,B., Yao,X., Zhang,C., Liu,Y., Wei,L., Huang,Q., Wang,M.,
            Zhang,Y., Hu,D. and Wu,W.
  TITLE     PTK6 inhibits autophagy to promote uveal melanoma tumorigenesis by
            binding to SOCS3 and regulating mTOR phosphorylation
  JOURNAL   Cell Death Dis 14 (1), 55 (2023)
   PUBMED   36690663
  REMARK    GeneRIF: PTK6 inhibits autophagy to promote uveal melanoma
            tumorigenesis by binding to SOCS3 and regulating mTOR
            phosphorylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2953)
  AUTHORS   Brauer,P.M., Zheng,Y., Evans,M.D., Dominguez-Brauer,C., Peehl,D.M.
            and Tyner,A.L.
  TITLE     The alternative splice variant of protein tyrosine kinase 6
            negatively regulates growth and enhances PTK6-mediated inhibition
            of beta-catenin
  JOURNAL   PLoS One 6 (3), e14789 (2011)
   PUBMED   21479203
  REMARK    GeneRIF: Alternative transcript-PTK6 is able to negatively regulate
            growth and modulate PTK6 activity, protein-protein associations
            and/or subcellular localization.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2953)
  AUTHORS   Mitchell,P.J., Barker,K.T., Shipley,J. and Crompton,M.R.
  TITLE     Characterisation and chromosome mapping of the human non receptor
            tyrosine kinase gene, brk
  JOURNAL   Oncogene 15 (12), 1497-1502 (1997)
   PUBMED   9333026
  REMARK    Erratum:[Oncogene 1998 Jul 9;17(1):129]
REFERENCE   7  (bases 1 to 2953)
  AUTHORS   Park,S.H., Lee,K.H., Kim,H. and Lee,S.T.
  TITLE     Assignment of the human PTK6 gene encoding a non-receptor protein
            tyrosine kinase to 20q13.3 by fluorescence in situ hybridization
  JOURNAL   Cytogenet Cell Genet 77 (3-4), 271-272 (1997)
   PUBMED   9284935
REFERENCE   8  (bases 1 to 2953)
  AUTHORS   Kamalati,T., Jolin,H.E., Mitchell,P.J., Barker,K.T., Jackson,L.E.,
            Dean,C.J., Page,M.J., Gusterson,B.A. and Crompton,M.R.
  TITLE     Brk, a breast tumor-derived non-receptor protein-tyrosine kinase,
            sensitizes mammary epithelial cells to epidermal growth factor
  JOURNAL   J Biol Chem 271 (48), 30956-30963 (1996)
   PUBMED   8940083
REFERENCE   9  (bases 1 to 2953)
  AUTHORS   Mitchell,P.J., Barker,K.T., Martindale,J.E., Kamalati,T.,
            Lowe,P.N., Page,M.J., Gusterson,B.A. and Crompton,M.R.
  TITLE     Cloning and characterisation of cDNAs encoding a novel non-receptor
            tyrosine kinase, brk, expressed in human breast tumours
  JOURNAL   Oncogene 9 (8), 2383-2390 (1994)
   PUBMED   8036022
REFERENCE   10 (bases 1 to 2953)
  AUTHORS   Lee,S.T., Strunk,K.M. and Spritz,R.A.
  TITLE     A survey of protein tyrosine kinase mRNAs expressed in normal human
            melanocytes
  JOURNAL   Oncogene 8 (12), 3403-3410 (1993)
   PUBMED   8247543
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121829.30, DA762278.1,
            BC035843.1 and X78549.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005975.3.
            
            Summary: The protein encoded by this gene is a cytoplasmic
            nonreceptor protein kinase which may function as an intracellular
            signal transducer in epithelial tissues. Overexpression of this
            gene in mammary epithelial cells leads to sensitization of the
            cells to epidermal growth factor and results in a partially
            transformed phenotype. Expression of this gene has been detected at
            low levels in some breast tumors but not in normal breast tissue.
            The encoded protein has been shown to undergo autophosphorylation.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X78549.1, BC035843.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000542869.3/ ENSP00000442460.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AL121829.30        66376-66381         c
            7-22                DA762278.1         1-16
            23-2198             BC035843.1         1-2176
            2199-2259           X78549.1           2165-2225
            2260-2953           AL121829.30        57006-57699         c
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     gene            1..2953
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /note="protein tyrosine kinase 6"
                     /db_xref="GeneID:5753"
                     /db_xref="HGNC:HGNC:9617"
                     /db_xref="MIM:602004"
     exon            1..292
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1418
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /EC_number="2.7.10.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     breast tumor kinase; protein-tyrosine kinase BRK;
                     tyrosine-protein kinase BRK; PTK6 protein tyrosine kinase
                     6"
                     /codon_start=1
                     /product="protein-tyrosine kinase 6 isoform 1"
                     /protein_id="NP_005966.1"
                     /db_xref="CCDS:CCDS13524.1"
                     /db_xref="GeneID:5753"
                     /db_xref="HGNC:HGNC:9617"
                     /db_xref="MIM:602004"
                     /translation="MVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQW
                     WWATLLDEAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGAF
                     LIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSL
                     SHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAI
                     KVISRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSLLELL
                     RDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLA
                     RLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMS
                     NHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENP
                     T"
     exon            293..414
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     exon            415..578
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     exon            579..732
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     exon            733..894
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1076
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1230
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..2953
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2931..2936
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /note="hexamer: AATAAA"
     polyA_site      2953
                     /gene="PTK6"
                     /gene_synonym="BRK"
                     /note="major polyA site"
ORIGIN      
        1 acgcccagct ctgggtgctc cagctgggcc acagcctggt cctgccgctg cgcccgcccg
       61 ccatggtgtc ccgggaccag gctcacctgg gccccaagta tgtgggcctc tgggacttca
      121 agtcccggac ggacgaggag ctgagcttcc gcgcggggga cgtcttccac gtggccagga
      181 aggaggagca gtggtggtgg gccacgctgc tggacgaggc gggtggggcc gtggcccagg
      241 gctatgtgcc ccacaactac ctggccgaga gggagacggt ggagtcggaa ccgtggttct
      301 ttggctgcat ctcccgctcg gaagctgtgc gtcggctgca ggccgagggc aacgccacgg
      361 gcgccttcct gatcagggtc agcgagaagc cgagtgccga ctacgtcctg tcggtgcggg
      421 acacgcaggc tgtgcggcac tacaagatct ggcggcgtgc cgggggccgg ctgcacctga
      481 acgaggcggt gtccttcctc agcctgcccg agcttgtgaa ctaccacagg gcccagagcc
      541 tgtcccacgg cctgcggctg gccgcgccct gccggaagca cgagcctgag cccctgcccc
      601 attgggatga ctgggagagg ccgagggagg agttcacgct ctgcaggaag ctggggtccg
      661 gctactttgg ggaggtcttc gaggggctct ggaaagaccg ggtccaggtg gccattaagg
      721 tgatttctcg agacaacctc ctgcaccagc agatgctgca gtcggagatc caggccatga
      781 agaagctgcg gcacaaacac atcctggcgc tgtacgccgt ggtgtccgtg ggggaccccg
      841 tgtacatcat cacggagctc atggccaagg gcagcctgct ggagctgctc cgcgactctg
      901 atgagaaagt cctgcccgtt tcggagctgc tggacatcgc ctggcaggtg gctgagggca
      961 tgtgttacct ggagtcgcag aattacatcc accgggacct ggccgccagg aacatcctcg
     1021 tcggggaaaa caccctctgc aaagttgggg acttcgggtt agccaggctt atcaaggagg
     1081 acgtctacct ctcccatgac cacaatatcc cctacaagtg gacggcccct gaagcgctct
     1141 cccgaggcca ttactccacc aaatccgacg tctggtcctt tgggattctc ctgcatgaga
     1201 tgttcagcag gggtcaggtg ccctacccag gcatgtccaa ccatgaggcc ttcctgaggg
     1261 tggacgccgg ctaccgcatg ccctgccctc tggagtgccc gcccagcgtg cacaagctga
     1321 tgctgacatg ctggtgcagg gaccccgagc agagaccctg cttcaaggcc ctgcgggaga
     1381 ggctctccag cttcaccagc tacgagaacc cgacctgagc tgctgtggag cgggcatggc
     1441 cgggccctgc tgaggagggg cctgggcaga gggcctggac ctgggatcaa ggcccacgcg
     1501 cttccctggg gtttactgag gtgatgggtg caggaaaggt tcacaaatgt ggagtgtctg
     1561 cgtccaatac acgcgtgtgc tcctctcctt actccatcgt gtgtgccttg ggtctcagct
     1621 gctgacacgc agcctgctct ggagcctgca gatgagatcc gggagactga cacgaagcca
     1681 gcagaggtca gaggggactc tgaccacagc ccgctctctg gctgtctgtc tgcagtgccc
     1741 ggctgagggt gggaggcaaa cacgccttgt tcctgctctt cccagttcag cttggtggga
     1801 gaaagtcatt cgcgtggctc gggacgctca tgtaaatttg gttttggtgc tcaagggttc
     1861 tttcctccca ggggcaggtg tttctttcct gtttgtcttg tgtcttgaga gcttggcctt
     1921 atgaccagtg agaactctct ccctggtctc tgccagccca agcatcactg cccgaggcgc
     1981 cagctcagtt tcaccgtcca cgtccacaag gggcttttcc caccttcacc tttgtcgctg
     2041 ggtcagtgct ggaaagcgcc cctcactcct gcgctgacaa gggcccttct ctactgtctg
     2101 tggggtggtt ccgggctggg ggggctgcct cctttgcacc tgattttgaa ggtgtctctt
     2161 tcatccatgg ttaagtcata aaaagcttat tggttttggt tttgactcac ctgaaagttt
     2221 ttttggttta aaagaagaat aggcggggca cggtggctca tgcctgtaat cccagcactt
     2281 tgggaggctg aggcaggtgg atcacgaggt caggagatcg acaccatcct ggctaacacg
     2341 gtgaaacccc gtctctacta aaaaatacaa aaaattagct gggtgtggtg gtgggggtgg
     2401 gcgcctgtag tcccagctac gtgggaggct gaggcagcag actggtgtga acccgggagg
     2461 tggagcttgc agtgagccga gatcgcgcca ctgcactcca gcctgggcga cagagcgaga
     2521 ctccatctca aaaaaaaaaa aaaaaaaaag aaagaaagaa aaataatttt gggagtttct
     2581 ggaaaggtac taggatttct caaaaggatt tgtcttctgc cttgtgaaag acagatgtca
     2641 gactaatcag gcttatccga tgtgctacat gagatggaaa gcgtgtgaaa tagtaagtca
     2701 cactaagtct tctggaggtt ctatttacgg gtttggtttt gatatgaatg ttgcagaaac
     2761 tctataaaat tcctagaaat ctgatacgtt atcctatgat atatatccta tctcacatgc
     2821 tattcctaga aatctggtgc aatgttatca gtcataattt tggttattac attaaaatgc
     2881 tgcatgccac agaaataacc aagtttcctt gccaaccgtg tcacgaatat aataaactct
     2941 catcagatcc tga
//
