LOCUS       NM_001146154            2765 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens prostaglandin reductase 2 (PTGR2), transcript variant
            2, mRNA.
ACCESSION   NM_001146154
VERSION     NM_001146154.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Chang,E.Y., Chang,Y.C., Shun,C.T., Tien,Y.W., Tsai,S.H., Hee,S.W.,
            Chen,I.J. and Chuang,L.M.
  TITLE     Inhibition of Prostaglandin Reductase 2, a Putative Oncogene
            Overexpressed in Human Pancreatic Adenocarcinoma, Induces Oxidative
            Stress-Mediated Cell Death Involving xCT and CTH Gene Expressions
            through 15-Keto-PGE2
  JOURNAL   PLoS One 11 (1), e0147390 (2016)
   PUBMED   26820738
  REMARK    GeneRIF: Silencing of PTGR2 expression enhances reactive oxygen
            species production, suppresses pancreatic cell proliferation, and
            promotes cell death through increasing 15-keto-PGE2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2765)
  AUTHORS   Chang,E.Y., Tsai,S.H., Shun,C.T., Hee,S.W., Chang,Y.C., Tsai,Y.C.,
            Tsai,J.S., Chen,H.J., Chou,J.W., Lin,S.Y. and Chuang,L.M.
  TITLE     Prostaglandin reductase 2 modulates ROS-mediated cell death and
            tumor transformation of gastric cancer cells and is associated with
            higher mortality in gastric cancer patients
  JOURNAL   Am J Pathol 181 (4), 1316-1326 (2012)
   PUBMED   22998775
  REMARK    GeneRIF: Functional data and clinical relevance for the role of
            PTGR2 in gastric cancer, are provided.
REFERENCE   4  (bases 1 to 2765)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2765)
  AUTHORS   Wu,Y.H., Ko,T.P., Guo,R.T., Hu,S.M., Chuang,L.M. and Wang,A.H.
  TITLE     Structural basis for catalytic and inhibitory mechanisms of human
            prostaglandin reductase PTGR2
  JOURNAL   Structure 16 (11), 1714-1723 (2008)
   PUBMED   19000823
  REMARK    GeneRIF: Besides targeting cyclooxygenase, indomethacin inhibits
            PTGR2 with a binding mode similar to that of 15-keto-PGE(2).
REFERENCE   6  (bases 1 to 2765)
  AUTHORS   Gao,K., Lockwood,W.W., Li,J., Lam,W. and Li,G.
  TITLE     Genomic analyses identify gene candidates for acquired irinotecan
            resistance in melanoma cells
  JOURNAL   Int J Oncol 32 (6), 1343-1349 (2008)
   PUBMED   18497997
REFERENCE   7  (bases 1 to 2765)
  AUTHORS   Chou,W.L., Chuang,L.M., Chou,C.C., Wang,A.H., Lawson,J.A.,
            FitzGerald,G.A. and Chang,Z.F.
  TITLE     Identification of a novel prostaglandin reductase reveals the
            involvement of prostaglandin E2 catabolism in regulation of
            peroxisome proliferator-activated receptor gamma activation
  JOURNAL   J Biol Chem 282 (25), 18162-18172 (2007)
   PUBMED   17449869
REFERENCE   8  (bases 1 to 2765)
  AUTHORS   Zhang,L., Zhang,F. and Huo,K.
  TITLE     Cloning and characterization of a novel splicing variant of the
            ZADH1 gene
  JOURNAL   Cytogenet Genome Res 103 (1-2), 79-83 (2003)
   PUBMED   15004468
  REMARK    GeneRIF: Cloning and tissue distribution of a splicing variant
            ZADH1b.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP357520.1, AC005520.2,
            AY346133.1 and BU616297.1.
            
            On Jul 10, 2019 this sequence version replaced NM_001146154.1.
            
            Summary: This gene encodes an enzyme involved in the metabolism of
            prostaglandins. The encoded protein catalyzes the NADPH-dependent
            conversion of 15-keto-prostaglandin E2 to
            15-keto-13,14-dihydro-prostaglandin E2. This protein may also be
            involved in regulating activation of the peroxisome
            proliferator-activated receptor. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Apr 2009].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, 3 and 4 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.4210160.1,
                                           SRR18074969.3771803.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000555661.6/ ENSP00000452280.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-111               BP357520.1         1-111
            112-112             AC005520.2         63297-63297         c
            113-148             AY346133.1         99-134
            149-149             AC005520.2         56388-56388         c
            150-387             AY346133.1         136-373
            388-388             AC005520.2         41145-41145         c
            389-714             AY346133.1         375-700
            715-715             AC005520.2         36108-36108         c
            716-1444            AY346133.1         702-1430
            1445-2010           AC005520.2         30268-30833         c
            2011-2498           BU616297.1         23-510              c
            2499-2765           AC005520.2         29513-29779         c
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.3"
     gene            1..2765
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="prostaglandin reductase 2"
                     /db_xref="GeneID:145482"
                     /db_xref="HGNC:HGNC:20149"
                     /db_xref="MIM:608642"
     exon            1..113
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            114..197
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1216
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /EC_number="1.3.1.48"
                     /note="isoform 1 is encoded by transcript variant 2;
                     15-oxoprostaglandin-delta13-reductase; zinc binding
                     alcohol dehydrogenase, domain containing 1; PRG-2;
                     15-oxoprostaglandin 13-reductase; zinc-binding alcohol
                     dehydrogenase domain-containing protein 1; epididymis
                     secretory protein Li 298; epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="prostaglandin reductase 2 isoform 1"
                     /protein_id="NP_001139626.1"
                     /db_xref="CCDS:CCDS9820.1"
                     /db_xref="GeneID:145482"
                     /db_xref="HGNC:HGNC:20149"
                     /db_xref="MIM:608642"
                     /translation="MIVQRVVLNSRPGKNGNPVAENFRMEEVYLPDNINEGQVQVRTL
                     YLSVDPYMRCRMNEDTGTDYITPWQLSQVVDGGGIGIIEESKHTNLTKGDFVTSFYWP
                     WQTKVILDGNSLEKVDPQLVDGHLSYFLGAIGMPGLTSLIGIQEKGHITAGSNKTMVV
                     SGAAGACGSVAGQIGHFLGCSRVVGICGTHEKCILLTSELGFDAAINYKKDNVAEQLR
                     ESCPAGVDVYFDNVGGNISDTVISQMNENSHIILCGQISQYNKDVPYPPPLSPAIEAI
                     QKERNITRERFLVLNYKDKFEPGILQLSQWFKEGKLKIKETVINGLENMGAAFQSMMT
                     GGNIGKQIVCISEEISL"
     exon            198..316
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            317..508
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            509..679
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            680..889
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1011
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1099
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1139
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..2765
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1673
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="major polyA site"
     regulatory      2477..2482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="hexamer: AGTAAA"
     polyA_site      2504
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="major polyA site"
     regulatory      2536..2541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="hexamer: AATATA"
     polyA_site      2554
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
     regulatory      2562..2567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="hexamer: AATAAG"
     polyA_site      2601
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
     regulatory      2746..2751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
                     /note="hexamer: AGTAAA"
     polyA_site      2765
                     /gene="PTGR2"
                     /gene_synonym="HEL-S-298; PGR2; ZADH1"
ORIGIN      
        1 gcgcgcctgc gtactgcggt cagagggccg aggcctgggg gcgagctggg gtcgtgcagt
       61 acagcctctt tccggcaaat cacgcgagat ttcgttcacc cgggctccac agggagtatt
      121 ttatacagaa atcttgtgaa accactgccc aaccagagca atgattgttc aaagagtggt
      181 attgaattct cgacctggaa aaaatggtaa tccagtggca gagaatttcc gaatggaaga
      241 agtctattta ccagataata ttaatgaagg acaagtacaa gttagaactc tttatctttc
      301 tgtggatcct tacatgcgtt gtagaatgaa tgaagacact ggcactgatt atataacacc
      361 ttggcagcta tctcaagtcg ttgatggagg aggtattgga attatagaag aaagcaaaca
      421 cacaaatttg actaaaggcg attttgtgac ttctttctat tggccctggc aaaccaaggt
      481 tattctggat ggaaatagcc ttgaaaaggt agacccacaa cttgtggatg gacacctttc
      541 atattttctt ggagctatag gtatgcctgg tttgacttcc ttgattggga tacaggaaaa
      601 aggtcatata actgctggat ctaataagac aatggttgtc agtggggccg caggtgcctg
      661 tggatctgtg gctgggcaga ttggccattt cttaggttgt tccagagtgg tgggaatttg
      721 tggaacacat gagaaatgca tcctcttgac ctcagaactg ggctttgatg ctgcaattaa
      781 ttataaaaaa gacaatgtgg cagaacagct ccgtgaatca tgcccagctg gagtggatgt
      841 ttattttgac aatgttggtg gtaacatcag tgatacagtg ataagtcaga tgaatgagaa
      901 cagccacatc atcctgtgtg gtcaaatttc tcagtacaac aaagatgtgc cttatcctcc
      961 cccgctatcc cctgctatag aggcaatcca gaaagaaaga aacatcacaa gggaaagatt
     1021 tctggtatta aattataaag acaaatttga gcctggcatt ctacagctga gtcagtggtt
     1081 taaagaagga aagctaaaga ttaaagagac ggtaataaat gggttggaaa acatgggagc
     1141 tgcattccag tccatgatga caggaggtaa cattggaaag cagatagttt gcatttcaga
     1201 agaaatctct ttgtaattgc tgtaaatgtc atcaaggcaa tcatagattt cttttccatt
     1261 ttgcatattt tcaaagatat gttaaaaaat ccttagacta tacatagctc ttgatttaaa
     1321 tgtgatcata ggtgttattt ttagttgcat agggtatttg atacaatcat taatggatca
     1381 tacacaatag gtttttaaaa attaataact tttagtaatt acttttatta atttaaaata
     1441 gaacgcttga gaggcacttt gtaaagattt gttaaactgg aaacgtttta catgatctga
     1501 tacaaccatt aatgaatcat acacaatagg ttttttaaaa ttaatattaa taacttttat
     1561 taatttaaaa tagaatgctt aaaataaaat agaatgcttg agaggcactg agtaaagatt
     1621 tgttgaactg gaaatgtttt acatgattct taaactgaaa cttggtgtaa aaatagaatt
     1681 gagatggcct ttttttcaca ttgtagactg aaaagagact taatggtatg atgtgtacat
     1741 agggactggg ggcaggattg ggggtttcgg agcttgtgta acagtttttg ggataggaga
     1801 ccagcggttt tgggttggga ttagtatggg aagataaata acttaggttg gttaagttga
     1861 caagatttac tccagaggat catctctttg tatttgccaa ataatttact gtatagccta
     1921 aaaactccat atatattgag aaaagcatat gtttatttta ggttagcagg cacatactgt
     1981 caagttgtaa agattgagag ggcaaacaga tgtaaacatc acttgtaggt gattaaaaag
     2041 attgacagcc gggcctgatg tctcaagcct gtaatcctag cactttggga ggcagaggcg
     2101 ggcagatcac ttgaggtcag gagttcaaga ccagcctggc caacacggtg aaaccccatc
     2161 tctctaaaaa tacaaaaatt agctgggcgt ggtggcacac gcctgtaatc ccagctactc
     2221 aggaggctga ggctggagaa tcatttgaac ctgggagggg gaggttgccg tgagctgagc
     2281 ttgcaccatt gcactccagc ctgggcgaca agagtgaaat tccatctcaa aaaacaaaaa
     2341 cagattgaca acaaagacag tttcagaaaa tgacaggact gggcaaatta acaaatgttt
     2401 gtaaacatga atgttcagga actactgatg tacctcaaaa gtttgtttta ttaattgtac
     2461 tcaaccctcg cagaacagta aaactgaaga ttattgtttc tgaatgtttt ggcttatgat
     2521 tgtctacaca ctgccaatat acttttgtat gcaacatttg taataagtag attcaaactg
     2581 tgttggtaat cagcctcccc agttttcgtg agtggccctt ttttgttact tttcctggac
     2641 catttcatcg cctgaggggg tttcactttt ctctgtatgt tgctaactcc caaatctcca
     2701 tctgtatttc tgacctcttt cttgagcact ggacctttac ctccaagtaa atatcctcaa
     2761 ctctg
//
