LOCUS       NM_000961               5570 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens prostaglandin I2 synthase (PTGIS), mRNA.
ACCESSION   NM_000961
VERSION     NM_000961.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ge,J., Zhou,Y., Li,H., Zeng,R., Xie,K., Leng,J., Chen,X., Yu,G.,
            Shi,X., Xu,Y., He,D., Guo,P., Zhou,Y., Luo,H., Luo,W. and Liu,B.
  TITLE     Prostacyclin Synthase Deficiency Leads to Exacerbation or
            Occurrence of Endothelium-Dependent Contraction and Causes
            Cardiovascular Disorders Mainly via the Non-TxA2 Prostanoids/TP
            Axis
  JOURNAL   Circ Res 135 (6), e133-e149 (2024)
   PUBMED   39082135
  REMARK    GeneRIF: Prostacyclin Synthase Deficiency Leads to Exacerbation or
            Occurrence of Endothelium-Dependent Contraction and Causes
            Cardiovascular Disorders Mainly via the Non-TxA2 Prostanoids/TP
            Axis.
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Chida-Nagai,A., Akagawa,H., Sawai,S., Ma,Y.J., Yakuwa,S.,
            Muneuchi,J., Yasuda,K., Yamazawa,H., Yamamoto,T., Takakuwa,E.,
            Tomaru,U., Furutani,Y., Kato,T., Harada,G., Inai,K., Nakanishi,T.,
            Manabe,A., Takeda,A. and Jing,Z.C.
  TITLE     Identification of Prostaglandin I2 Synthase Rare Variants in
            Patients With Williams Syndrome and Severe Peripheral Pulmonary
            Stenosis
  JOURNAL   J Am Heart Assoc 13 (9), e032872 (2024)
   PUBMED   38639351
  REMARK    GeneRIF: Identification of Prostaglandin I2 Synthase Rare Variants
            in Patients With Williams Syndrome and Severe Peripheral Pulmonary
            Stenosis.
REFERENCE   3  (bases 1 to 5570)
  AUTHORS   Wang,B., Ge,S., Wang,Z., Wang,W., Wang,Y., Leng,H. and Ma,X.
  TITLE     Analysis and experimental validation of fatty acid
            metabolism-related genes prostacyclin synthase (PTGIS) in
            endometrial cancer
  JOURNAL   Aging (Albany NY) 15 (19), 10322-10346 (2023)
   PUBMED   37796199
  REMARK    GeneRIF: Analysis and experimental validation of fatty acid
            metabolism-related genes prostacyclin synthase (PTGIS) in
            endometrial cancer.
REFERENCE   4  (bases 1 to 5570)
  AUTHORS   Ding,H., Wang,K.Y., Chen,S.Y., Guo,K.W. and Qiu,W.H.
  TITLE     Validating the role of PTGIS gene in colorectal cancer by
            bioinformatics analysis and in vitro experiments
  JOURNAL   Sci Rep 13 (1), 16496 (2023)
   PUBMED   37779109
  REMARK    GeneRIF: Validating the role of PTGIS gene in colorectal cancer by
            bioinformatics analysis and in vitro experiments.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5570)
  AUTHORS   Qiao,D., Liu,Y., Lei,Y., Zhang,C., Bu,Y., Tang,Y. and Zhang,Y.
  TITLE     rRNA-Derived Small RNA rsRNA-28S Regulates the Chemoresistance of
            Prostate Cancer Cells by Targeting PTGIS
  JOURNAL   Front Biosci (Landmark Ed) 28 (5), 102 (2023)
   PUBMED   37258478
  REMARK    GeneRIF: rRNA-Derived Small RNA rsRNA-28S Regulates the
            Chemoresistance of Prostate Cancer Cells by Targeting PTGIS.
REFERENCE   6  (bases 1 to 5570)
  AUTHORS   Nelson,D.R., Zeldin,D.C., Hoffman,S.M., Maltais,L.J., Wain,H.M. and
            Nebert,D.W.
  TITLE     Comparison of cytochrome P450 (CYP) genes from the mouse and human
            genomes, including nomenclature recommendations for genes,
            pseudogenes and alternative-splice variants
  JOURNAL   Pharmacogenetics 14 (1), 1-18 (2004)
   PUBMED   15128046
  REMARK    Review article
REFERENCE   7  (bases 1 to 5570)
  AUTHORS   Wang,L.H. and Chen,L.
  TITLE     Organization of the gene encoding human prostacyclin synthase
  JOURNAL   Biochem Biophys Res Commun 226 (3), 631-637 (1996)
   PUBMED   8831668
REFERENCE   8  (bases 1 to 5570)
  AUTHORS   Yokoyama,C., Yabuki,T., Inoue,H., Tone,Y., Hara,S., Hatae,T.,
            Nagata,M., Takahashi,E.I. and Tanabe,T.
  TITLE     Human gene encoding prostacyclin synthase (PTGIS): genomic
            organization, chromosomal localization, and promoter activity
  JOURNAL   Genomics 36 (2), 296-304 (1996)
   PUBMED   8812456
REFERENCE   9  (bases 1 to 5570)
  AUTHORS   Nakayama,T., Soma,M., Izumi,Y. and Kanmatsuse,K.
  TITLE     Organization of the human prostacyclin synthase gene
  JOURNAL   Biochem Biophys Res Commun 221 (3), 803-806 (1996)
   PUBMED   8630042
  REMARK    Erratum:[Biochem Biophys Res Commun. 1996 Jul 5;224(1):281. doi:
            10.1006/bbrc.1996.1021.. PMID: 8694828]
REFERENCE   10 (bases 1 to 5570)
  AUTHORS   Miyata,A., Hara,S., Yokoyama,C., Inoue,H., Ullrich,V. and Tanabe,T.
  TITLE     Molecular cloning and expression of human prostacyclin synthase
  JOURNAL   Biochem Biophys Res Commun 200 (3), 1728-1734 (1994)
   PUBMED   8185632
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D83402.2, AF297048.1, D38145.1
            and AL118525.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000961.3.
            
            Summary: This gene encodes a member of the cytochrome P450
            superfamily of enzymes. The cytochrome P450 proteins are
            monooxygenases which catalyze many reactions involved in drug
            metabolism and synthesis of cholesterol, steroids and other lipids.
            However, this protein is considered a member of the cytochrome P450
            superfamily on the basis of sequence similarity rather than
            functional similarity. This endoplasmic reticulum membrane protein
            catalyzes the conversion of prostglandin H2 to prostacyclin
            (prostaglandin I2), a potent vasodilator and inhibitor of platelet
            aggregation. An imbalance of prostacyclin and its physiological
            antagonist thromboxane A2 contribute to the development of
            myocardial infarction, stroke, and atherosclerosis. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.511141.1,
                                           SRR14372080.4329853.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000244043.5/ ENSP00000244043.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                D83402.2           1491-1511
            22-1524             AF297048.1         1-1503
            1525-1971           D38145.1           1531-1977
            1972-5570           AL118525.17        15617-19215         c
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.13"
     gene            1..5570
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /note="prostaglandin I2 synthase"
                     /db_xref="GeneID:5740"
                     /db_xref="HGNC:HGNC:9603"
                     /db_xref="MIM:601699"
     exon            1..95
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1524
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /EC_number="5.3.99.4"
                     /EC_number="4.2.1.152"
                     /note="cytochrome P450, family 8, subfamily A, polypeptide
                     1; prostaglandin I2 (prostacyclin) synthase; hydroperoxy
                     icosatetraenoate dehydratase"
                     /codon_start=1
                     /product="prostacyclin synthase"
                     /protein_id="NP_000952.1"
                     /db_xref="CCDS:CCDS13419.1"
                     /db_xref="GeneID:5740"
                     /db_xref="HGNC:HGNC:9603"
                     /db_xref="MIM:601699"
                     /translation="MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYAL
                     DFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIF
                     LMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWH
                     EMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLA
                     RGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARA
                     LVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKV
                     LDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQ
                     RDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNS
                     IKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP"
     exon            96..219
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            220..398
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            399..542
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            543..694
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            695..876
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            877..1045
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1227
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1379
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..5570
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5547..5552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /note="hexamer: ATTAAA"
     polyA_site      5570
                     /gene="PTGIS"
                     /gene_synonym="CYP8; CYP8A1; PGIS; PTGI"
                     /note="major polyA site"
ORIGIN      
        1 gccagccccg ccagccccgc gatggcttgg gccgcgctcc tcggcctcct ggccgcactg
       61 ttgctgctgc tgctactgag ccgccgccgc acgcggcgac ctggtgagcc tcccctggac
      121 ctgggcagca tcccctggtt ggggtatgcc ttggactttg gaaaagatgc tgccagcttc
      181 ctcacgagga tgaaggagaa gcacggtgac atctttacta tactggttgg gggcaggtat
      241 gtcaccgttc tcctggaccc acactcctac gacgcggtgg tgtgggagcc tcgcaccagg
      301 ctcgacttcc atgcctatgc catcttcctc atggagagga tttttgatgt gcagcttcca
      361 cattacagcc ccagtgatga aaaggccagg atgaaactga ctcttctcca cagagagctc
      421 caggcactca cagaagccat gtataccaac ctccatgcag tgctgttggg cgatgctaca
      481 gaagcaggca gtggctggca cgagatgggt ctcctcgact tctcctacag cttcctgctc
      541 agagccggct acctgactct ttacggaatt gaggcgctgc cacgcaccca tgaaagccag
      601 gcccaggacc gcgtccactc agctgatgtc ttccacacct ttcgccagct cgaccggctg
      661 ctccccaaac tggcccgtgg ctccctgtca gtgggggaca aggaccacat gtgcagtgtc
      721 aaaagtcgcc tgtggaagct gctatcccca gccaggctgg ccaggcgggc ccaccggagc
      781 aaatggctgg agagttacct gctgcacctg gaggagatgg gtgtgtcaga ggagatgcag
      841 gcacgggccc tggtgctgca gctgtgggcc acacagggga atatgggtcc cgctgccttc
      901 tggctcctgc tcttccttct caagaatcct gaagccctgg ctgctgtccg cggagagctc
      961 gagagtatcc tttggcaagc ggagcagcct gtctcgcaga cgaccactct cccacagaag
     1021 gttctagaca gcacacctgt gcttgatagc gtgctgagtg agagcctcag gcttacagct
     1081 gcccccttca tcacccgcga ggttgtggtg gacctggcca tgcccatggc agacgggcga
     1141 gaattcaacc tgcgacgtgg tgaccgcctc ctcctcttcc ccttcctgag cccccagaga
     1201 gacccagaaa tctacacaga cccagaggta tttaaataca accgattcct gaaccctgac
     1261 ggatcagaga agaaagactt ttacaaggat gggaaacggc tgaagaatta caacatgccc
     1321 tggggggcgg ggcacaatca ctgcctgggg aggagttatg cggtcaacag catcaaacaa
     1381 tttgtgttcc ttgtgctggt gcacttggac ttggagctga tcaacgcaga tgtggagatc
     1441 cctgagtttg acctcagcag gtacggcttc ggtctgatgc agccggaaca cgacgtgccc
     1501 gtccgctacc gcatccgccc atgacacagg gagcagatgg atccacgtgc tcgcctctgc
     1561 ccagcctgcc ccagcctgcc ccagcctccc agctttctgt gtgcacagtt ggcccgggtg
     1621 caggtgctag cattaccact tccctgcttt tctcccagaa ggctgggtcc aggggaggga
     1681 aaagctaaga gggtgaacaa agaaaagaca ttgaaagctc tatggattat ccactgcaaa
     1741 gttttctttc caaaatcagg ctttgtctgc tcccaattca cctcgttact ctcacctcgt
     1801 gatatccaca aatgctattc agataaggca gaactaggag tcttcactgc tctgccccca
     1861 actcccggag gtgtcacctt cctagttctt atgagctagc atggcccggg ccttatccag
     1921 tcaaagcgga tgctggccac agaaaggcca ctcaggatgt cctttgtgtc cattgatgtc
     1981 attcagcagt cagtccccca ataatcctta aactagctaa aaccaaagga gtcccttaga
     2041 agatctgctt ccctggggcc ccatttgcca gattgcccca ttgctcacac tacttgagaa
     2101 aatgcaggag agcttccccc aaggctgatg cattcccggt gcagaacagg ggcaccctcc
     2161 aaacactggg ctctgaggag tggagttctc tgttctagag tgacaggcac cagatgggat
     2221 gggctttctc agtgtcagca ctcaggtagg gagctaagga agacacagcc cagacaagat
     2281 ggctggaagg agccagccag gactccttag actgatcaag ccaaaaaaga aggtgccgat
     2341 ttcatgcatt ctagtgcaga agccccaact gtgatcacga tccagtctgc agacgtgttt
     2401 tgtttggact tcacttaaaa aaatgcctta gttgttatca tctttgggag agttcattca
     2461 aaatgtccag cttctcttga aaacttggta tatctggcca cactgggctc acattcccaa
     2521 gggtaactct tggccagagc tgagtggcag ccgcctccct tatgcaggac atgtgctctc
     2581 ggcttcacca gggttctgac cgggtctgct tctgcattca cagcgcctcc tggacctgaa
     2641 ggcatctgag tgtgagaccc tgttctaact cttagaagtg acattgtaag aggtggtggg
     2701 gaccagctaa ttggtccaac ccagcctgag tgcaccaccc tttgaacaaa tgtatcagtg
     2761 atgaaaattt gcctttgccc cggcttgcct gtaatcccag cactttggga ggccgaggtg
     2821 ggcggatcac ttgaggtcgg gagttcaaga ccagcctggc caacatggcg aaaccccgtc
     2881 tctactaaac ataaaaaaat tagtcaggtg tggcggtgcg tgcctgtaat cccagctatt
     2941 caggaggctg aggcaccaga attgcttgaa cccaggaggt ggaggttgca gtgaactgag
     3001 actgcgccac ggcactccag cctgggcgac agagcaagac tctgtctcaa taaataaata
     3061 attaattaaa ataaaaaaca gcttaaagag aaaaatggcc tgcaaacctt ttttatgatg
     3121 ctatttttat taatataaag tcctgtttat tgagaccctt taaatgcctg cgagagaccc
     3181 tacagacagt atgtctactc ctcacagcat ctctatgaag agaaggaggg ttgtgcccac
     3241 ttcatagatg agaaaactga gaggtgaggt gacttgcctg gggccacata gctcataagc
     3301 tgtagaactc tgcaggcaga tttactgtcc caggagcaaa tgctggatga gcaactcctg
     3361 ttctttgggc tcaaggggac tggtgatggg acaattcttc tcgacttcag gagctgacag
     3421 agccagaggc acctaaactt gggtacatct tgtaagacac ataatgaggt ccctctagct
     3481 ttagctggag ggagataaaa gaaccccaga cctctgaatg tcccagaggc tagtctcttc
     3541 tcagagcagc actggggttt gggggcttcc ctgggcctca gcctccaggc acccccagga
     3601 ttcccagaga gacgctgtga ttggcaggag gcaggatatc cccagggaaa cccatcttca
     3661 cgcctgggtg acccccactg ccgcctcctt cttaactctg caggaaatca gagctggcag
     3721 cctccagtgg gaggacagag ccggtttccg tggcaaccct cagctgcctc atcgtggctg
     3781 ggaagggaag gaagcaagcc ggcagaaata gctatggaag gtcttgcgca ggctgcaacc
     3841 ctggtgtgct gggcgaaaac ccttatgact ccccccttcc aaatcaggct gggttgtcac
     3901 tgagactaga ttctcacctg ccttcaaaga agggccaatt ccctttaaag gtcgcacctc
     3961 cttggaacca cagtcattag tgaattacac tcaaggaaaa gatgtgctcc caccaggcag
     4021 ctccagctgt tacctgagat actgaagtgc agctcaggag acgattattt aaacctgccc
     4081 ttgttttaat cgttattttt ctctttaaaa aaaatagaag ctataaagaa aagagggaga
     4141 gatgagtggg ttagctacct gctatgcgct agttaggaag ttacctggat gccattgtat
     4201 ttcttcatcc cttgcttaag aatcaaaatt actggacata tgttaggaat actctttctt
     4261 tctttctttc tttttaagct cagtggcaag aatgaaatac tctttttttt tttttttttt
     4321 tgagatggag tctcgctctg ctgcccaggc tagagtgcag tggcgtgatc tcggctcact
     4381 gcaagctctg cctcccgtgt tcacaccatt ctcctgcctc agcctcctga gtagctggga
     4441 ctacaggcac ccgccaccac acccggctaa tttttggatt tttagtagag atgggatttc
     4501 accgtattag ccaggatggt cttgatctcc tgagctcgtg atctgcccgc ctcggcctcc
     4561 caaagtgctg ggattacagg tgtgagccac cgcgcccagc caagaataaa atactcttaa
     4621 gttgatctaa tgaagtgttt ccttaccatt gtgattattg ttactattat ttgctatatt
     4681 ttaatattgt tgtttaccaa atattctcct ttaaacagac tcgcttttta aacttttttt
     4741 tttttttttg agacggagtt tcacgcttat tgcccaggct ggagtgcaat ggcacgatct
     4801 cagctcacca caacctccac ctcctgggtt taagcaatgc ttctgcctca gcttcccaag
     4861 tagctgagat tacaggcgca caccaccacg cccaactgat ttttgtattt ttagtagaga
     4921 cggggtttcc ccatgttggt caggctggtc tcgaactcct gacctcgtga tttgcctgcc
     4981 tgggcctccc aaagtgctag gattacaggc atgagccacc atgcccggcc taaactttgt
     5041 ttttaaaatg aacttttttt ccccccaatt gctgccaata gtggataaca tgtatcactc
     5101 actgccaaaa atagaaagtg accatgaaaa ataaattcgc tggggaaggg ggctccatgc
     5161 tggtgtggcc aaggctgaga gctctctctt ctctgttaca aaacgagata agcaagtgtt
     5221 agaattgcct taaggccaca ctggcatctc cctgaccttc tccagggaca gaagcaggag
     5281 taagtttctc atcccatggg cgaccagggc catctcctcc caccagtggc ccccactcac
     5341 agggagctgg caatgcccta cctgcctgtt ctccagatgg agaaacaggc tctgagattt
     5401 cacaggtctt gcccaaagtc attgattttg atgattaaaa agaataaaca cagtgtttcc
     5461 tgagtagcag tgattgttat gccttgctat tttaataaag attctatttt cgtataacat
     5521 tgtcaagtgg aaacatgctg aaatctatta aaccatcttt gtttgtggaa
//
