LOCUS       NM_000958               3431 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens prostaglandin E receptor 4 (PTGER4), mRNA.
ACCESSION   NM_000958
VERSION     NM_000958.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Chen,H., Zhou,Q., Pu,X., Wang,N., Wang,S., Feng,Z., Wang,B.,
            Zhu,Z., Qiu,Y. and Sun,X.
  TITLE     Association between vertebral endplate defects and patient-reported
            symptoms: an immunohistochemical study investigating the
            COX-2/PGE-2/EP-4 axis
  JOURNAL   Spine J 24 (8), 1407-1415 (2024)
   PUBMED   38631491
  REMARK    GeneRIF: Association between vertebral endplate defects and
            patient-reported symptoms: an immunohistochemical study
            investigating the COX-2/PGE-2/EP-4 axis.
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Lavoie,J.C., Simard,M., Kalkan,H., Rakotoarivelo,V., Huot,S., Di
            Marzo,V., Cote,A., Pouliot,M. and Flamand,N.
  TITLE     Pharmacological evidence that the inhibitory effects of
            prostaglandin E2 are mediated by the EP2 and EP4 receptors in human
            neutrophils
  JOURNAL   J Leukoc Biol 115 (6), 1183-1189 (2024)
   PUBMED   38345417
  REMARK    GeneRIF: Pharmacological evidence that the inhibitory effects of
            prostaglandin E2 are mediated by the EP2 and EP4 receptors in human
            neutrophils.
REFERENCE   3  (bases 1 to 3431)
  AUTHORS   Ishikawa,S., Umemura,M., Nakakaji,R., Nagasako,A., Nagao,K.,
            Mizuno,Y., Sugiura,K., Kioi,M., Mitsudo,K. and Ishikawa,Y.
  TITLE     EP4-induced mitochondrial localization and cell migration mediated
            by CALML6 in human oral squamous cell carcinoma
  JOURNAL   Commun Biol 7 (1), 567 (2024)
   PUBMED   38745046
  REMARK    GeneRIF: EP4-induced mitochondrial localization and cell migration
            mediated by CALML6 in human oral squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3431)
  AUTHORS   Qiu,J., Li,Q., Li,J., Zhou,F., Sang,P., Xia,Z., Wang,W., Wang,L.,
            Yu,Y. and Jiang,J.
  TITLE     Complementary roles of EP2 and EP4 receptors in malignant glioma
  JOURNAL   Br J Pharmacol 180 (20), 2623-2640 (2023)
   PUBMED   37232020
  REMARK    GeneRIF: Complementary roles of EP2 and EP4 receptors in malignant
            glioma.
REFERENCE   5  (bases 1 to 3431)
  AUTHORS   Yu,S., Tu,R., Chen,Z., Song,J., Li,P., Hu,F., Yuan,G., Zhang,R. and
            Li,Y.
  TITLE     Association of PTGER4 and PRKAA1 genetic polymorphisms with gastric
            cancer
  JOURNAL   BMC Med Genomics 16 (1), 209 (2023)
   PUBMED   37670284
  REMARK    GeneRIF: Association of PTGER4 and PRKAA1 genetic polymorphisms
            with gastric cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3431)
  AUTHORS   Foord,S.M., Marks,B., Stolz,M., Bufflier,E., Fraser,N.J. and
            Lee,M.G.
  TITLE     The structure of the prostaglandin EP4 receptor gene and related
            pseudogenes
  JOURNAL   Genomics 35 (1), 182-188 (1996)
   PUBMED   8661119
REFERENCE   7  (bases 1 to 3431)
  AUTHORS   Duncan,A.M., Anderson,L.L., Funk,C.D., Abramovitz,M. and Adam,M.
  TITLE     Chromosomal localization of the human prostanoid receptor gene
            family
  JOURNAL   Genomics 25 (3), 740-742 (1995)
   PUBMED   7759114
REFERENCE   8  (bases 1 to 3431)
  AUTHORS   Regan,J.W., Bailey,T.J., Pepperl,D.J., Pierce,K.L., Bogardus,A.M.,
            Donello,J.E., Fairbairn,C.E., Kedzie,K.M., Woodward,D.F. and
            Gil,D.W.
  TITLE     Cloning of a novel human prostaglandin receptor with
            characteristics of the pharmacologically defined EP2 subtype
  JOURNAL   Mol Pharmacol 46 (2), 213-220 (1994)
   PUBMED   8078484
REFERENCE   9  (bases 1 to 3431)
  AUTHORS   Bastien,L., Sawyer,N., Grygorczyk,R., Metters,K.M. and Adam,M.
  TITLE     Cloning, functional expression, and characterization of the human
            prostaglandin E2 receptor EP2 subtype
  JOURNAL   J Biol Chem 269 (16), 11873-11877 (1994)
   PUBMED   8163486
REFERENCE   10 (bases 1 to 3431)
  AUTHORS   An,S., Yang,J., Xia,M. and Goetzl,E.J.
  TITLE     Cloning and expression of the EP2 subtype of human receptors for
            prostaglandin E2
  JOURNAL   Biochem Biophys Res Commun 197 (1), 263-270 (1993)
   PUBMED   8250933
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093264.2, BC027934.1,
            L28175.1, BM312677.1, CD000197.1 and BQ029720.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000958.2.
            
            Summary: The protein encoded by this gene is a member of the
            G-protein coupled receptor family. This protein is one of four
            receptors identified for prostaglandin E2 (PGE2). This receptor can
            activate T-cell factor signaling. It has been shown to mediate PGE2
            induced expression of early growth response 1 (EGR1), regulate the
            level and stability of cyclooxygenase-2 mRNA, and lead to the
            phosphorylation of glycogen synthase kinase-3. Knockout studies in
            mice suggest that this receptor may be involved in the neonatal
            adaptation of circulatory system, osteoporosis, as well as
            initiation of skin immune responses. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.2343316.1,
                                           SRR11853567.23867.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302472.4/ ENSP00000302846.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AC093264.2         70962-70976         c
            16-564              BC027934.1         1-549
            565-2177            L28175.1           346-1958
            2178-2640           BM312677.1         73-535
            2641-3205           CD000197.1         143-707
            3206-3431           BQ029720.1         17-242              c
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.1"
     gene            1..3431
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /note="prostaglandin E receptor 4"
                     /db_xref="GeneID:5734"
                     /db_xref="HGNC:HGNC:9596"
                     /db_xref="MIM:601586"
     exon            1..564
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /inference="alignment:Splign:2.1.0"
     exon            565..1474
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /inference="alignment:Splign:2.1.0"
     CDS             608..2074
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /note="PGE receptor, EP4 subtype; prostanoid EP4 receptor;
                     PGE2 receptor EP4 subtype; prostaglandin E receptor 4
                     (subtype EP4)"
                     /codon_start=1
                     /product="prostaglandin E2 receptor EP4 subtype"
                     /protein_id="NP_000949.1"
                     /db_xref="CCDS:CCDS3930.1"
                     /db_xref="GeneID:5734"
                     /db_xref="HGNC:HGNC:9596"
                     /db_xref="MIM:601586"
                     /translation="MSTPGVNSSASLSPDRLNSPVTIPAVMFIFGVVGNLVAIVVLCK
                     SRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYMKGQWPGGQPLCEYSTFILLFF
                     SLSGLSIICAMSVERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPNMGLGS
                     SRLQYPDTWCFIDWTTNVTAHAAYSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFM
                     RRTSLGTEQHHAAAAASVASRGHPAASPALPRLSDFRRRRSFRRIAGAEIQMVILLIA
                     TSLVVLICSIPLVVRVFVNQLYQPSLEREVSKNPDLQAIRIASVNPILDPWIYILLRK
                     TVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTS
                     QTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSES
                     VLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI"
     exon            1475..3431
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3413..3418
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /note="hexamer: AATAAA"
     polyA_site      3431
                     /gene="PTGER4"
                     /gene_synonym="EP4; EP4R"
                     /note="major polyA site"
ORIGIN      
        1 acagagggtg gaaaggcgag agcggagctc caagcccggc agcccgagag gaagatgaac
       61 agccccaggc cagagcctct ggcagagtgg accccgagcc gcccccaggt agccaggagc
      121 ggcctcagcg gcagccgcaa actccagtag ccgcccgtgc tgcccgtggc tggggcggag
      181 ggcagccaga gctggggacc aaggctccgc gccacctgcg cgcacagcct cacacctgaa
      241 cgctgtcctc ccgcagacga gaccggcggg cactgcaaag ctgggactcg tctttgaagg
      301 aaaaaaaata gcgagtaaga aatccagcac cattcttcac tgacccatcc cgctgcacct
      361 cttgtttccc aagtttttga aagctggcaa ctctgacctc ggtgtccaaa aatcgacagc
      421 cactgagacc ggctttgaga agccgaagat ttggcagttt ccagactgag caggacaagg
      481 tgaaagcagg ttggaggcgg gtccaggaca tctgagggct gaccctgggg gctcgtgagg
      541 ctgccaccgc tgctgccgct acagacccag ccttgcactc caaggctgcg caccgccagc
      601 cactatcatg tccactcccg gggtcaattc gtccgcctcc ttgagccccg accggctgaa
      661 cagcccagtg accatcccgg cggtgatgtt catcttcggg gtggtgggca acctggtggc
      721 catcgtggtg ctgtgcaagt cgcgcaagga gcagaaggag acgaccttct acacgctggt
      781 atgtgggctg gctgtcaccg acctgttggg cactttgttg gtgagcccgg tgaccatcgc
      841 cacgtacatg aagggccaat ggcccggggg ccagccgctg tgcgagtaca gcaccttcat
      901 tctgctcttc ttcagcctgt ccggcctcag catcatctgc gccatgagtg tcgagcgcta
      961 cctggccatc aaccatgcct atttctacag ccactacgtg gacaagcgat tggcgggcct
     1021 cacgctcttt gcagtctatg cgtccaacgt gctcttttgc gcgctgccca acatgggtct
     1081 cggtagctcg cggctgcagt acccagacac ctggtgcttc atcgactgga ccaccaacgt
     1141 gacggcgcac gccgcctact cctacatgta cgcgggcttc agctccttcc tcattctcgc
     1201 caccgtcctc tgcaacgtgc ttgtgtgcgg cgcgctgctc cgcatgcacc gccagttcat
     1261 gcgccgcacc tcgctgggca ccgagcagca ccacgcggcc gcggccgcct cggttgcctc
     1321 ccggggccac cccgctgcct ccccagcctt gccgcgcctc agcgactttc ggcgccgccg
     1381 gagcttccgc cgcatcgcgg gcgccgagat ccagatggtc atcttactca ttgccacctc
     1441 cctggtggtg ctcatctgct ccatcccgct cgtggtgcga gtattcgtca accagttata
     1501 tcagccaagt ttggagcgag aagtcagtaa aaatccagat ttgcaggcca tccgaattgc
     1561 ttctgtgaac cccatcctag acccctggat atatatcctc ctgagaaaga cagtgctcag
     1621 taaagcaata gagaagatca aatgcctctt ctgccgcatt ggcgggtccc gcagggagcg
     1681 ctccggacag cactgctcag acagtcaaag gacatcttct gccatgtcag gccactctcg
     1741 ctccttcatc tcccgggagc tgaaggagat cagcagtaca tctcagaccc tcctgccaga
     1801 cctctcactg ccagacctca gtgaaaatgg ccttggaggc aggaatttgc ttccaggtgt
     1861 gcctggcatg ggcctggccc aggaagacac cacctcactg aggactttgc gaatatcaga
     1921 gacctcagac tcttcacagg gtcaggactc agagagtgtc ttactggtgg atgaggctgg
     1981 tgggagcggc agggctgggc ctgcccctaa ggggagctcc ctgcaagtca catttcccag
     2041 tgaaacactg aacttatcag aaaaatgtat ataataggca aggaaagaaa tacagtactg
     2101 tttctggacc cttataaaat cctgtgcaat agacacatac atgtcacatt tagctgtgct
     2161 cagaagggct atcatcatcc tacaactcac attagagaac atcctggctt ttgagcactt
     2221 ttcaaacaat caagttgact cacgtgggtc ctgaggcctg cagcacgtcg gatgctaccc
     2281 cactatgaca gaggattgtg gtcacaactt gatggctgcg aagacctacc ctccgttttt
     2341 ctactagata ggaggatggt agaagtttgg ctgctgtcat aacatccaga gctttgtcgt
     2401 atttggcaca cagcagaggc ccagatatta gaaaggctct attccaataa actatgagga
     2461 ctgccttatg gatgatttaa gtgtctcact aaagcatgaa atgtgaattt ttattgttgt
     2521 acatacgatt taaggtattt aaagtatttt cttctctgtg agaaggttta ttgttaatac
     2581 aaggtataat aaaattatcg caacccctct ccttccagta taaccagctg aagttgcaga
     2641 tgttagatat ttttcataaa caagttcgag tcaaagttga aaattcatag taagattgat
     2701 atctataaaa tagatataaa tttttaagag aaagaattta gtattatcaa agggataaag
     2761 aaaaaaatac tatttaagat gtgaaaatta cagtccaaaa tactgttctt tccaggctat
     2821 gtataaaata catagtgaaa attgtttagt gatattacat ttatttatcc agaaaactgt
     2881 gatttcagga gaacctaaca tgctggtgaa tattttcaac tttttccctc actaattggt
     2941 acttttaaaa acataacata aattttttga agtctttaat aaataaccca taattgaagt
     3001 gtataatata aaaaatttta aaaatctaag cagcttattg tttctctgaa agtgtgtgta
     3061 gttttacttt cctaaggaat taccaagaat atcctttaaa atttaaaagg atggcaagtt
     3121 gcatcagaaa gctttatttt gagatgtaaa aagattccca aacgtggtta cattagccat
     3181 tcatgtatgt cagaagtgca gaattggggc acttaatggt caccttgtaa cagttttgtg
     3241 taactcccag tgatgctgta cacatatttg aagggtcttt ctcaaagaaa tattaagcat
     3301 gttttgttgc tcagtgtttt tgtgaattgc ttggttgtaa ttaaattctg agcctgatat
     3361 tgatatggtt ttaagaagca gttgtaccaa gtgaaattat tttggagatt ataataaata
     3421 tatacattca a
//
