LOCUS       NM_000264               8662 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.
ACCESSION   NM_000264
VERSION     NM_000264.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Patel,H.V., Joshi,J.S. and Shah,F.D.
  TITLE     Implicating clinical utility of altered expression of PTCH1 & SMO
            in oral squamous cell carcinoma
  JOURNAL   J Mol Histol 55 (4), 379-389 (2024)
   PUBMED   38954185
  REMARK    GeneRIF: Implicating clinical utility of altered expression of
            PTCH1 & SMO in oral squamous cell carcinoma.
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Yu,P., Yang,J. and Zhang,Y.
  TITLE     Identification and characterization of two novel PTCH1 splice
            variants
  JOURNAL   Biochem Biophys Res Commun 487 (1), 68-75 (2017)
   PUBMED   28390897
  REMARK    GeneRIF: Thus, our study clearly demonstrated the unique
            involvement of the two novel PTCH1 splice variants in HH signal
            transduction.
REFERENCE   3  (bases 1 to 8662)
  AUTHORS   Wu,F., Zhang,Y., Sun,B., McMahon,A.P. and Wang,Y.
  TITLE     Hedgehog Signaling: From Basic Biology to Cancer Therapy
  JOURNAL   Cell Chem Biol 24 (3), 252-280 (2017)
   PUBMED   28286127
  REMARK    Review article
REFERENCE   4  (bases 1 to 8662)
  AUTHORS   Shimokawa,T., Svard,J., Heby-Henricson,K., Teglund,S., Toftgard,R.
            and Zaphiropoulos,P.G.
  TITLE     Distinct roles of first exon variants of the tumor-suppressor
            Patched1 in Hedgehog signaling
  JOURNAL   Oncogene 26 (34), 4889-4896 (2007)
   PUBMED   17310997
REFERENCE   5  (bases 1 to 8662)
  AUTHORS   Nagao,K., Toyoda,M., Takeuchi-Inoue,K., Fujii,K., Yamada,M. and
            Miyashita,T.
  TITLE     Identification and characterization of multiple isoforms of a
            murine and human tumor suppressor, patched, having distinct first
            exons
  JOURNAL   Genomics 85 (4), 462-471 (2005)
   PUBMED   15780749
  REMARK    GeneRIF: Seven isoforms of human PTCH mRNA were identified.
REFERENCE   6  (bases 1 to 8662)
  AUTHORS   Johnson,R.L., Rothman,A.L., Xie,J., Goodrich,L.V., Bare,J.W.,
            Bonifas,J.M., Quinn,A.G., Myers,R.M., Cox,D.R., Epstein,E.H. Jr.
            and Scott,M.P.
  TITLE     Human homolog of patched, a candidate gene for the basal cell nevus
            syndrome
  JOURNAL   Science 272 (5268), 1668-1671 (1996)
   PUBMED   8658145
REFERENCE   7  (bases 1 to 8662)
  AUTHORS   Hahn,H., Christiansen,J., Wicking,C., Zaphiropoulos,P.G.,
            Chidambaram,A., Gerrard,B., Vorechovsky,I., Bale,A.E., Toftgard,R.,
            Dean,M. and Wainwright,B.
  TITLE     A mammalian patched homolog is expressed in target tissues of sonic
            hedgehog and maps to a region associated with developmental
            abnormalities
  JOURNAL   J Biol Chem 271 (21), 12125-12128 (1996)
   PUBMED   8647801
REFERENCE   8  (bases 1 to 8662)
  AUTHORS   Muller,E. II and Hudgins,L.
  TITLE     9q22.3 Microdeletion - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21850767
REFERENCE   9  (bases 1 to 8662)
  AUTHORS   Tekendo-Ngongang,C., Muenke,M. and Kruszka,P.
  TITLE     Holoprosencephaly Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301702
REFERENCE   10 (bases 1 to 8662)
  AUTHORS   Evans,D.G.
  TITLE     Nevoid Basal Cell Carcinoma Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL161729.27.
            
            On Jul 26, 2019 this sequence version replaced NM_000264.4.
            
            Summary: This gene encodes a member of the patched family of
            proteins and a component of the hedgehog signaling pathway.
            Hedgehog signaling is important in embryonic development and
            tumorigenesis. The encoded protein is the receptor for the secreted
            hedgehog ligands, which include sonic hedgehog, indian hedgehog and
            desert hedgehog. Following binding by one of the hedgehog ligands,
            the encoded protein is trafficked away from the primary cilium,
            relieving inhibition of the G-protein-coupled receptor smoothened,
            which results in activation of downstream signaling. Mutations of
            this gene have been associated with basal cell nevus syndrome and
            holoprosencephaly. [provided by RefSeq, Aug 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2692930.1,
                                           SRR18074969.1188707.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000331920.11/ ENSP00000332353.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1106              AL161729.27        51586-52691
            1107-1299           AL161729.27        54253-54445
            1300-1489           AL161729.27        74978-75167
            1490-1559           AL161729.27        78649-78718
            1560-1651           AL161729.27        78812-78903
            1652-1850           AL161729.27        80264-80462
            1851-1972           AL161729.27        80762-80883
            1973-2120           AL161729.27        81705-81852
            2121-2252           AL161729.27        82666-82797
            2253-2408           AL161729.27        83150-83305
            2409-2507           AL161729.27        83995-84093
            2508-2633           AL161729.27        84693-84818
            2634-2752           AL161729.27        90921-91039
            2753-3155           AL161729.27        91699-92101
            3156-3465           AL161729.27        93427-93736
            3466-3608           AL161729.27        98854-98996
            3609-3792           AL161729.27        101069-101252
            3793-4073           AL161729.27        102559-102839
            4074-4211           AL161729.27        104439-104576
            4212-4354           AL161729.27        107232-107374
            4355-4454           AL161729.27        110912-111011
            4455-4709           AL161729.27        111529-111783
            4710-5250           AL161729.27        113401-113941
            5251-8662           AL161729.27        114461-117872
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.32"
     gene            1..8662
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /note="patched 1"
                     /db_xref="GeneID:5727"
                     /db_xref="HGNC:HGNC:9585"
                     /db_xref="MIM:601309"
     exon            1..1106
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1299
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1489
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1559
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1651
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1850
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1972
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2120
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            2121..2252
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2408
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2507
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            2508..2633
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..2752
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..3155
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            3156..3465
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            3466..3608
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            3609..3792
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            3793..4073
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            4074..4211
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            4212..4354
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            4355..4454
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            4455..4709
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            4710..5250
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     exon            5251..8662
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8204..8209
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /note="hexamer: AATAAA"
     polyA_site      8231
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /note="major polyA site"
     regulatory      8640..8645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
                     /note="hexamer: AATAAA"
     polyA_site      8662
                     /gene="PTCH1"
                     /gene_synonym="BCNS; BCNS1; NBCCS; PTC; PTC1; PTCH"
ORIGIN      
        1 accccctccc ctcccctcct cctcttcttt ctccaaatgg agagagttct tttttttctt
       61 cttccatctc atctattgaa tcaaggagct gctgcggccg ctgcccgctg cacacacaca
      121 gagcggagtc cccaggtccc gggagcgaga gaggcgcgct gcacggggac agaatggtcc
      181 agcgggttgc cgaggagcac aagaaagcag agtccgggac cgagcagcca ccgcgaaccc
      241 agcagccaga gcccgagcag cccgagcagc agctcctgga gccgccaccg ccagcagcag
      301 ccaccgcggg agcagcggca gctgcggctg cccgggcccg gcagcgctga gacccgccgg
      361 gcaccctcgg accccgcggc ggcggcggct ctgcgcttgc cctccgcggc cgctcgggcg
      421 acccgggagg cgccgagaga gccagcgcgg ccggcgggag cagcggggat tcgctggctc
      481 tttctgcagt gaaggggtcg cggcgcgggg cggggggcgg gtagggggag ttggggaccg
      541 caaggagtgc cgcggaagcg cccgaaggac aggctcgctc ggcgcgccgg ctctcgctct
      601 tccgcgaact ggatgtgggc agcggcggcc gcagagacct cgggaccccc gcgcaatgtg
      661 gcaatggaag gcgcagggtc tgactccccg gcagcggccg cggccgcagc ggcagcagcg
      721 cccgccgtgt gagcagcagc agcggctggt ctgtcaaccg gagcccgagc ccgagcagcc
      781 tgcggccagc agcgtcctcg caagccgagc gcccaggcgc gccaggagcc cgcagcagcg
      841 gcagcagcgc gccgggccgc ccgggaagcc tccgtccccg cggcggcggc ggcggcggcg
      901 gcaacatggc ctcggctggt aacgccgccg agccccagga ccgcggcggc ggcggcagcg
      961 gctgtatcgg tgccccggga cggccggctg gaggcgggag gcgcagacgg acgggggggc
     1021 tgcgccgtgc tgccgcgccg gaccgggact atctgcaccg gcccagctac tgcgacgccg
     1081 ccttcgctct ggagcagatt tccaagggga aggctactgg ccggaaagcg ccgctgtggc
     1141 tgagagcgaa gtttcagaga ctcttattta aactgggttg ttacattcaa aaaaactgcg
     1201 gcaagttctt ggttgtgggc ctcctcatat ttggggcctt cgcggtggga ttaaaagcag
     1261 cgaacctcga gaccaacgtg gaggagctgt gggtggaagt tggaggacga gtaagtcgtg
     1321 aattaaatta tactcgccag aagattggag aagaggctat gtttaatcct caactcatga
     1381 tacagacccc taaagaagaa ggtgctaatg tcctgaccac agaagcgctc ctacaacacc
     1441 tggactcggc actccaggcc agccgtgtcc atgtatacat gtacaacagg cagtggaaat
     1501 tggaacattt gtgttacaaa tcaggagagc ttatcacaga aacaggttac atggatcaga
     1561 taatagaata tctttaccct tgtttgatta ttacaccttt ggactgcttc tgggaagggg
     1621 cgaaattaca gtctgggaca gcatacctcc taggtaaacc tcctttgcgg tggacaaact
     1681 tcgacccttt ggaattcctg gaagagttaa agaaaataaa ctatcaagtg gacagctggg
     1741 aggaaatgct gaataaggct gaggttggtc atggttacat ggaccgcccc tgcctcaatc
     1801 cggccgatcc agactgcccc gccacagccc ccaacaaaaa ttcaaccaaa cctcttgata
     1861 tggcccttgt tttgaatggt ggatgtcatg gcttatccag aaagtatatg cactggcagg
     1921 aggagttgat tgtgggtggc acagtcaaga acagcactgg aaaactcgtc agcgcccatg
     1981 ccctgcagac catgttccag ttaatgactc ccaagcaaat gtacgagcac ttcaaggggt
     2041 acgagtatgt ctcacacatc aactggaacg aggacaaagc ggcagccatc ctggaggcct
     2101 ggcagaggac atatgtggag gtggttcatc agagtgtcgc acagaactcc actcaaaagg
     2161 tgctttcctt caccaccacg accctggacg acatcctgaa atccttctct gacgtcagtg
     2221 tcatccgcgt ggccagcggc tacttactca tgctcgccta tgcctgtcta accatgctgc
     2281 gctgggactg ctccaagtcc cagggtgccg tggggctggc tggcgtcctg ctggttgcac
     2341 tgtcagtggc tgcaggactg ggcctgtgct cattgatcgg aatttccttt aacgctgcaa
     2401 caactcaggt tttgccattt ctcgctcttg gtgttggtgt ggatgatgtt tttcttctgg
     2461 cccacgcctt cagtgaaaca ggacagaata aaagaatccc ttttgaggac aggaccgggg
     2521 agtgcctgaa gcgcacagga gccagcgtgg ccctcacgtc catcagcaat gtcacagcct
     2581 tcttcatggc cgcgttaatc ccaattcccg ctctgcgggc gttctccctc caggcagcgg
     2641 tagtagtggt gttcaatttt gccatggttc tgctcatttt tcctgcaatt ctcagcatgg
     2701 atttatatcg acgcgaggac aggagactgg atattttctg ctgttttaca agcccctgcg
     2761 tcagcagagt gattcaggtt gaacctcagg cctacaccga cacacacgac aatacccgct
     2821 acagcccccc acctccctac agcagccaca gctttgccca tgaaacgcag attaccatgc
     2881 agtccactgt ccagctccgc acggagtacg acccccacac gcacgtgtac tacaccaccg
     2941 ctgagccgcg ctccgagatc tctgtgcagc ccgtcaccgt gacacaggac accctcagct
     3001 gccagagccc agagagcacc agctccacaa gggacctgct ctcccagttc tccgactcca
     3061 gcctccactg cctcgagccc ccctgtacga agtggacact ctcatctttt gctgagaagc
     3121 actatgctcc tttcctcttg aaaccaaaag ccaaggtagt ggtgatcttc ctttttctgg
     3181 gcttgctggg ggtcagcctt tatggcacca cccgagtgag agacgggctg gaccttacgg
     3241 acattgtacc tcgggaaacc agagaatatg actttattgc tgcacaattc aaatactttt
     3301 ctttctacaa catgtatata gtcacccaga aagcagacta cccgaatatc cagcacttac
     3361 tttacgacct acacaggagt ttcagtaacg tgaagtatgt catgttggaa gaaaacaaac
     3421 agcttcccaa aatgtggctg cactacttca gagactggct tcagggactt caggatgcat
     3481 ttgacagtga ctgggaaacc gggaaaatca tgccaaacaa ttacaagaat ggatcagacg
     3541 atggagtcct tgcctacaaa ctcctggtgc aaaccggcag ccgcgataag cccatcgaca
     3601 tcagccagtt gactaaacag cgtctggtgg atgcagatgg catcattaat cccagcgctt
     3661 tctacatcta cctgacggct tgggtcagca acgaccccgt cgcgtatgct gcctcccagg
     3721 ccaacatccg gccacaccga ccagaatggg tccacgacaa agccgactac atgcctgaaa
     3781 caaggctgag aatcccggca gcagagccca tcgagtatgc ccagttccct ttctacctca
     3841 acggcttgcg ggacacctca gactttgtgg aggcaattga aaaagtaagg accatctgca
     3901 gcaactatac gagcctgggg ctgtccagtt accccaacgg ctaccccttc ctcttctggg
     3961 agcagtacat cggcctccgc cactggctgc tgctgttcat cagcgtggtg ttggcctgca
     4021 cattcctcgt gtgcgctgtc ttccttctga acccctggac ggccgggatc attgtgatgg
     4081 tcctggcgct gatgacggtc gagctgttcg gcatgatggg cctcatcgga atcaagctca
     4141 gtgccgtgcc cgtggtcatc ctgatcgctt ctgttggcat aggagtggag ttcaccgttc
     4201 acgttgcttt ggcctttctg acggccatcg gcgacaagaa ccgcagggct gtgcttgccc
     4261 tggagcacat gtttgcaccc gtcctggatg gcgccgtgtc cactctgctg ggagtgctga
     4321 tgctggcggg atctgagttc gacttcattg tcaggtattt ctttgctgtg ctggcgatcc
     4381 tcaccatcct cggcgttctc aatgggctgg ttttgcttcc cgtgcttttg tctttctttg
     4441 gaccatatcc tgaggtgtct ccagccaacg gcttgaaccg cctgcccaca ccctcccctg
     4501 agccaccccc cagcgtggtc cgcttcgcca tgccgcccgg ccacacgcac agcgggtctg
     4561 attcctccga ctcggagtat agttcccaga cgacagtgtc aggcctcagc gaggagcttc
     4621 ggcactacga ggcccagcag ggcgcgggag gccctgccca ccaagtgatc gtggaagcca
     4681 cagaaaaccc cgtcttcgcc cactccactg tggtccatcc cgaatccagg catcacccac
     4741 cctcgaaccc gagacagcag ccccacctgg actcagggtc cctgcctccc ggacggcaag
     4801 gccagcagcc ccgcagggac ccccccagag aaggcttgtg gccacccccc tacagaccgc
     4861 gcagagacgc ttttgaaatt tctactgaag ggcattctgg ccctagcaat agggcccgct
     4921 ggggccctcg cggggcccgt tctcacaacc ctcggaaccc agcgtccact gccatgggca
     4981 gctccgtgcc cggctactgc cagcccatca ccactgtgac ggcttctgcc tccgtgactg
     5041 tcgccgtgca cccgccgcct gtccctgggc ctgggcggaa cccccgaggg ggactctgcc
     5101 caggctaccc tgagactgac cacggcctgt ttgaggaccc ccacgtgcct ttccacgtcc
     5161 ggtgtgagag gagggattcg aaggtggaag tcattgagct gcaggacgtg gaatgcgagg
     5221 agaggccccg gggaagcagc tccaactgag ggtgattaaa atctgaagca aagaggccaa
     5281 agattggaaa ccccccaccc ccacctcttt ccagaactgc ttgaagagaa ctggttggag
     5341 ttatggaaaa gatgccctgt gccaggacag cagttcattg ttactgtaac cgattgtatt
     5401 attttgttaa atatttctat aaatatttaa gagatgtaca catgtgtaat ataggaagga
     5461 aggatgtaaa gtggtatgat ctggggcttc tccactcctg ccccagagtg tggaggccac
     5521 agtggggcct ctccgtattt gtgcattggg ctccgtgcca caaccaagct tcattagtct
     5581 taaatttcag catatgttgc tgctgcttaa atattgtata atttacttgt ataattctat
     5641 gcaaatattg cttatgtaat aggattattt tgtaaaggtt tctgtttaaa atattttaaa
     5701 tttgcatatc acaaccctgt ggtagtatga aatgttactg ttaactttca aacacgctat
     5761 gcgtgataat ttttttgttt aatgagcaga tatgaagaaa gcacgttaat cctggtggct
     5821 tctctaggtg tcgttgtgtg cggtcctctt gtttggctgt gcgtgtgaac acgtgtgtga
     5881 gttcaccatg tactgtactg tgattttttt tttgtcttgt tttgtttctc tacactgtct
     5941 gtaacctgta gtaggctctg acctagtcag gctggaagcg tcaggatatc ttttcttcgt
     6001 gctggtgagg gctggcccta aacatccacc taatcctttc aaatcagccc ggcaaaagct
     6061 agactctcct cgtgtctacg gcatctctta tgatcattgg ctgccatcca ggaccccaat
     6121 ttgtgcttca gggggataat ctccttctct cggatcattg tgatggatgc tggaacctca
     6181 gggtatggag ctcacatcag ttcatcatgg tgggtgttag agaattcggt gacatgccta
     6241 gtgctgagcc ttggctgggc catgagagtc tgtatactct aaaaagcatg cagcatggtg
     6301 cccctcttct gaccaacaca cacacgaccc ctcccccaac acccccaaat tcaagagtgg
     6361 atgtggccct gtcacaggta gaaaaaccta tttagttaat tctttcttgg cccacagtct
     6421 cccagaaatg atgttttgag tccctatagt ttaaactccc tctcttaaat ggagcagctg
     6481 gttgaggctt tctagatctg tttgcatctt ctttaaaact aagtggtgag catgcattgt
     6541 ggtgtagagg caggcattat gtaggataag agctccgggg ggattcttca tgcaccagtg
     6601 tttagggtac gtgcttccta agtaaatcca aacattgtct ccatcctccc cgtcattagt
     6661 gctctttcaa tgtgatgtgg gaaagcagga ggatggacac accccactga aagatgtagg
     6721 caggggcagg tctctcaacc aggcatattt ttaaaagttg cttctgtact ggttctcttc
     6781 ttttgctctg aggtgtgggc tccctcatct cgtaaccaga gaccagcaca tgtcagggaa
     6841 gcacccagtg tcggctcccc atccaaatcc acaccagcac cttgttacag acaagaagtc
     6901 agaggaaagg gcggggtccc tgcagggctg aagcctaagc tactgtgagg cgctcacgag
     6961 tggcagctcc tgttactccc ttttaaatta cctgggaaat cttaacagaa aggtaatggg
     7021 cccccagaaa tacccacagc atagtgacct cagaccctga tactcaccac aaaactttta
     7081 agatgctgat tgggagccgc ttgtggctgc tgggtgtgtg tgtgtgtgtg tgcgtgcgtg
     7141 cgtgtgtgtg tgtctctgct ggggaccctg gccacccccc tgctgctgtc ttggtgcctg
     7201 tcacccacat ggtctgccat cctaacaccc agctctgctc agaaaacgtc ctgcgtggag
     7261 gagggatgat gcagaattct gaagtcgact tccctctggc tcctggcgtg ccctcgctcc
     7321 cttcctgagc ccagctcgtg ttgcgccgga ggctgcgcgg cccctgattt ctgcatggtg
     7381 tagaactttc tccaatagtc acattggcaa agggagaact ggggtgggcg gggggtgggg
     7441 ctggcaggga attagaattt ctctctctct tttaatagtt ttattttgtc tgtcctgttt
     7501 gttcatttgg atgttttaat ttttaaaaaa aaaaaaactt tgctgatatt tataattttg
     7561 tatcataaga atgttttcct ctacagtatt tgtcatgcca gtttataaca aaaaaaaatg
     7621 cagggatttt atttctattg gaaacattac agctatgttt tacttttgga cagaattttt
     7681 atttgtatag agtgcttact aatgttaaat agttcagagt atataacatt tacattaagg
     7741 actcatggta ggttttaggg taaggagttt aaaggaaata aatattcaaa ctgggtctca
     7801 ttgccaattt tggtggaaat gagtttgtgt catttcaatt acaaagataa aagtatgcca
     7861 tataatttat ttatatgaag atttattttt gtagtgtaca tagtagtcat caagtctttt
     7921 gacagaagta tatttttaaa gaatttatat gtgatgaatc cataatgtct ggaactttgc
     7981 tgagacatga gtgggcacag ttttcattgt aaattacagc aaggaaagaa aatgtttaac
     8041 agtgttaaga gagtcagagc agagtggata ttcatgcgat tatgaagtgt ttattagtta
     8101 ccattggcga cctagcatgc ttctcatttc aaaccttgga aggtgaaaat gtacaaactc
     8161 tctaaataat taatgttcaa acactgatag aaattctaac atgaataaaa aataatataa
     8221 cttgttggtt atatctttgt ttgtagaatg ctttttttct ttaaaatact tgggagagac
     8281 agttagtgtt ggagccatca gaaactgttg tttcatcttg ctgaccttgt gacactcttc
     8341 ttttttgctc tatctgatgg agagagtttt aggttttcct cctttttgtt ttgtataaat
     8401 agtgggtata agcatagctc tgttacatgg atgtattaca aagtgggggc tgagctttta
     8461 gtgtgatcat cacccaaata gtgtacattg tacccattaa ttaatttctt atcacctgac
     8521 cctatttttg ttttctcata ccaagaaact tcctaagtta accatcaaaa ttagtccttc
     8581 tgtcatctct cttagcatac ttatttccct tatgtgatgc ctaataatga gggaacataa
     8641 ataaagccaa attcaaagat ga
//
