LOCUS       NM_017952               6681 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pentatricopeptide repeat domain 3 (PTCD3), mRNA.
ACCESSION   NM_017952
VERSION     NM_017952.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Munoz-Pujol,G., Ortigoza-Escobar,J.D., Paredes-Fuentes,A.J.,
            Jou,C., Ugarteburu,O., Gort,L., Yubero,D., Garcia-Cazorla,A.,
            O'Callaghan,M., Campistol,J., Muchart,J., Yepez,V.A., Gusic,M.,
            Gagneur,J., Prokisch,H., Artuch,R., Ribes,A., Urreizti,R. and
            Tort,F.
  TITLE     Leigh syndrome is the main clinical characteristic of PTCD3
            deficiency
  JOURNAL   Brain Pathol 33 (3), e13134 (2023)
   PUBMED   36450274
  REMARK    GeneRIF: Leigh syndrome is the main clinical characteristic of
            PTCD3 deficiency.
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 6681)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6681)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6681)
  AUTHORS   Borna,N.N., Kishita,Y., Kohda,M., Lim,S.C., Shimura,M., Wu,Y.,
            Mogushi,K., Yatsuka,Y., Harashima,H., Hisatomi,Y., Fushimi,T.,
            Ichimoto,K., Murayama,K., Ohtake,A. and Okazaki,Y.
  TITLE     Mitochondrial ribosomal protein PTCD3 mutations cause oxidative
            phosphorylation defects with Leigh syndrome
  JOURNAL   Neurogenetics 20 (1), 9-25 (2019)
   PUBMED   30607703
  REMARK    GeneRIF: Sanger sequencing of gDNA from the affected individual and
            unaffected family members confirming the PTCD3 variants
REFERENCE   6  (bases 1 to 6681)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   7  (bases 1 to 6681)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 6681)
  AUTHORS   Davies,S.M., Rackham,O., Shearwood,A.M., Hamilton,K.L., Narsai,R.,
            Whelan,J. and Filipovska,A.
  TITLE     Pentatricopeptide repeat domain protein 3 associates with the
            mitochondrial small ribosomal subunit and regulates translation
  JOURNAL   FEBS Lett 583 (12), 1853-1858 (2009)
   PUBMED   19427859
  REMARK    GeneRIF: lowering PTCD3 in 143B osteosarcoma cells decreased
            mitochondrial protein synthesis, mitochondrial respiration and the
            activity of Complexes III and IV, suggesting that PTCD3 has a role
            in mitochondrial translation
            GeneRIF: PTCD3 associates with the small subunit of the
            mitochondrial ribosome and is required for efficient translation.
REFERENCE   9  (bases 1 to 6681)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   10 (bases 1 to 6681)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA479719.1, BC011832.2, AC009309.4 and BQ574414.1.
            
            On Nov 24, 2018 this sequence version replaced NM_017952.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.262796.1,
                                           SRR1803611.179978.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000254630.12/
                                                  ENSP00000254630.7
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                DA479719.1         54-65
            13-2109             BC011832.2         1-2097
            2110-6582           AC009309.4         78516-82988         c
            6583-6681           BQ574414.1         19-117              c
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..6681
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /note="pentatricopeptide repeat domain 3"
                     /db_xref="GeneID:55037"
                     /db_xref="HGNC:HGNC:24717"
                     /db_xref="MIM:614918"
     exon            1..117
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..2083
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /note="pentatricopeptide repeat-containing protein 3,
                     mitochondrial; TRG-15; transformation-related gene 15
                     protein; pentatricopeptide repeat domain-containing
                     protein 3, mitochondrial; 28S ribosomal protein S39,
                     mitochondrial; mitochondrial small ribosomal subunit
                     protein mS39"
                     /codon_start=1
                     /product="small ribosomal subunit protein mS39 precursor"
                     /protein_id="NP_060422.4"
                     /db_xref="CCDS:CCDS33235.1"
                     /db_xref="GeneID:55037"
                     /db_xref="HGNC:HGNC:24717"
                     /db_xref="MIM:614918"
                     /translation="MAVVSAVRWLGLRSRLGQPLTGRRAGLCEQARSCRFYSGSATLS
                     KVEGTDVTGIEEVVIPKKKTWDKVAVLQALASTVNRDTTAVPYVFQDDPYLMPASSLE
                     SRSFLLAKKSGENVAKFIINSYPKYFQKDIAEPHIPCLMPEYFEPQIKDISEAALKER
                     IELRKVKASVDMFDQLLQAGTTVSLETTNSLLDLLCYYGDQEPSTDYHFQQTGQSEAL
                     EEENDETSRRKAGHQFGVTWRAKNNAERIFSLMPEKNEHSYCTMIRGMVKHRAYEQAL
                     NLYTELLNNRLHADVYTFNALIEATVCAINEKFEEKWSKILELLRHMVAQKVKPNLQT
                     FNTILKCLRRFHVFARSPALQVLREMKAIGIEPSLATYHHIIRLFDQPGDPLKRSSFI
                     IYDIMNELMGKRFSPKDPDDDKFFQSAMSICSSLRDLELAYQVHGLLKTGDNWKFIGP
                     DQHRNFYYSKFFDLICLMEQIDVTLKWYEDLIPSAYFPHSQTMIHLLQALDVANRLEV
                     IPKIWKDSKEYGHTFRSDLREEILMLMARDKHPPELQVAFADCAADIKSAYESQPIRQ
                     TAQDWPATSLNCIAILFLRAGRTQEAWKMLGLFRKHNKIPRSELLNELMDSAKVSNSP
                     SQAIEVVELASAFSLPICEGLTQRVMSDFAINQEQKEALSNLTALTSDSDTDSSSDSD
                     SDTSEGK"
     exon            118..170
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            171..207
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            208..253
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            254..322
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            323..427
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            428..551
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            552..667
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            668..729
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            730..817
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            818..878
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            879..964
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1109
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1160
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1250
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1279
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1386
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1465
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1556
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1642
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1791
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1833
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1992
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..6681
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2680..2685
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /note="hexamer: AATATA"
     polyA_site      2718
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /note="major polyA site"
     regulatory      6226..6231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /note="hexamer: AGTAAA"
     polyA_site      6252
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
     polyA_site      6624
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
     regulatory      6663..6668
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
                     /note="hexamer: AATAAA"
     polyA_site      6681
                     /gene="PTCD3"
                     /gene_synonym="COXPD51; MRP-S39; mS39"
ORIGIN      
        1 agagaaatca aagatggcgg ttgtatctgc tgttcgctgg ctgggcctcc gcagcaggct
       61 tggccagccg ctgacgggtc ggcgggcggg tttgtgtgaa caggcacgca gctgcagatt
      121 ttattctggt agtgcaaccc tctcaaaggt tgaaggaact gatgtaacag ggattgaaga
      181 agtagtaatt ccaaaaaaga aaacttggga taaagtagcc gttcttcagg cacttgcatc
      241 cacagtaaac agggatacca cagctgtgcc ttatgtgttt caagatgatc cttaccttat
      301 gccagcatca tctttggaat ctcgttcatt tttactggca aagaaatccg gggagaatgt
      361 ggccaagttt attattaatt cataccccaa atattttcag aaggacatag ctgaacctca
      421 tataccgtgt ttaatgcctg agtactttga acctcagatc aaagacataa gtgaagccgc
      481 cctgaaggaa cgaattgagc tcagaaaagt caaagcctct gtggacatgt ttgatcagct
      541 tttgcaagca ggaaccactg tgtctcttga aacaacaaat agtctcttgg atttattgtg
      601 ttactatggt gaccaggagc cctcaactga ttaccatttt caacaaactg gacagtcaga
      661 agcattggaa gaggaaaatg atgagacatc taggaggaaa gctggtcatc agtttggagt
      721 tacatggcga gcaaaaaaca acgctgagag aatcttttct ctaatgccag agaaaaatga
      781 acattcctat tgcacaatga tccgaggaat ggtgaagcac cgagcttatg agcaggcatt
      841 aaacttgtac actgagttac taaacaacag actccatgct gatgtataca catttaatgc
      901 attgattgaa gcaacagtat gtgcgataaa tgagaaattt gaggaaaaat ggagtaaaat
      961 actggagctg ctaagacaca tggttgcaca gaaggtgaaa ccaaatcttc agacttttaa
     1021 taccattctg aaatgtctcc gaagatttca tgtgtttgca agatcgccag ccttacaggt
     1081 tttacgtgaa atgaaagcca ttggaataga accctcgctt gcaacatatc accatattat
     1141 tcgcctgttt gatcaacctg gagacccttt aaagagatca tccttcatca tttatgatat
     1201 aatgaatgaa ttaatgggaa agagattttc tccaaaggac ccggatgatg ataagttttt
     1261 tcagtcagcc atgagcatat gctcatctct cagagatcta gaacttgcct accaagtaca
     1321 tggcctttta aaaaccggag acaactggaa attcattgga cctgatcaac atcgtaattt
     1381 ctattattcc aagttcttcg atttgatttg tctaatggaa caaattgatg ttaccttgaa
     1441 gtggtatgag gacctgatac cttcagccta ctttccccac tcccaaacaa tgatacatct
     1501 tctccaagca ttggatgtgg ccaatcggct agaagtgatt cctaaaattt ggaaagatag
     1561 taaagaatat ggtcatactt tccgcagtga cctgagagaa gagatcctga tgctcatggc
     1621 aagggacaag cacccaccag agcttcaggt ggcatttgct gactgtgctg ctgatatcaa
     1681 atctgcgtat gaaagccaac ccatcagaca gactgctcag gattggccag ccacctctct
     1741 caactgtata gctatcctct ttttaagggc tgggagaact caggaagcct ggaaaatgtt
     1801 ggggcttttc aggaagcata ataagattcc tagaagtgag ttgctgaatg agcttatgga
     1861 cagtgcaaaa gtgtctaaca gcccttccca ggccattgaa gtagtagagc tggcaagtgc
     1921 cttcagctta cctatttgtg agggcctcac ccagagagta atgagtgatt ttgcaatcaa
     1981 ccaggaacaa aaggaagccc taagtaatct aactgcattg accagtgaca gtgatactga
     2041 cagcagcagt gacagcgaca gtgacaccag tgaaggcaaa tgaaagtgga gattcaggag
     2101 cagcaatggg tctcaccata gctgctggaa tcacacctga gaactgagat ataccaatat
     2161 ttaacattgt tacaaagaag aaaagataca gatttggtga atttgttact gtgaggtaca
     2221 gtcagtacac agctgactta tgtagattta agctgctaat atgctactta accatctatt
     2281 aatgcaccat taaaggctta gcatttaagt agcaacattg cggttttcag acacatggtg
     2341 aggtccatgg ctcttgtcat caggataagc ctgcacacct agagtgtcgg tgagctgacc
     2401 tcacgatgct gtcctcgtgc gattgccctc tcctgctgct ggacttctgc ctttgttggc
     2461 ctgatgtgct gctgtgatgc tggtccttca tcttaggtgt tcatgcagtt ctaacacagt
     2521 tggggttggg tcaatagttt cccaatttca ggatatttcg atgtcagaaa taacgcatct
     2581 taggaatgac taaacaagat aatggcagtt taggctgcac aactggtaaa atgactgtag
     2641 ataaatgttg taattagtgt acacgtttgt atttttgtta atatagccgc tgccatagtt
     2701 ttctaacttg aacagccatg aatgtttcat gtctcccttt ttttttgtct atagctgtta
     2761 cctattttag tggttgaaat gagagctagt gatgacagaa ggatgtggaa tgtcttcttg
     2821 acatcattgt gtattgctgg taatcaagtt ggtaacgact acttctagca gctcttacca
     2881 ctatgactta agtggtcctg gaaggcagta agtggaggtt tgcagcattc ctgccttcat
     2941 gagggcttct accactgacc actttgcacg tacctggctc ccagatttac ttaggtaccc
     3001 cacgagttgt ccacataagc agcttcatct ttaccttgcc agagttgaca attatgggat
     3061 actctagtct acttatactt gtgttcccat ctgtctgcca tcctctgaag gccgggaccc
     3121 agtcatacat ccttagaaac caaagtatgg tttttgtttt ctcttggaat gtcaggtctt
     3181 aaggcattta attgagggac aaaaaaaaaa aaagccgata tagtagctag ctacttaagc
     3241 atccatgggt attgctccat atcaaagcag atttgcagga cagagagagt aaattagcct
     3301 tcagtcttgg tttacagctt ccaaggagag ccttggccac ctgaaatgtt aactcggtcc
     3361 cttcctgtct ctagttcatc agcacctgca gatgcctgac tcttgttagc cttactattc
     3421 aatacagtcc ttagattcac ggtatgcctc ttcctatcca ggcacctatt ctgaatcacc
     3481 atgttgctct gcagctagag ttgataggag aaaatccatt tgggtagatg gcctatgaat
     3541 ttgtagtaga ctttcaaaat gagtgatttg ttagcttggt acttttaagt ttgtggtaca
     3601 gatcctccaa acccatactc tgagcaatta actgccttga acatagagaa aaattaaggc
     3661 ctcacaggat gagtctccat tctctgtaaa tgcttatttt atcatagtct ttagcctcta
     3721 ctatgagtaa aatgttctct tcggccgggt gtggtgactc acacctgtaa cctcagcact
     3781 ttgggaggca gaggtgggag gatcacttag gtccaggagt tcgagactag cctgggcaac
     3841 atagtgagac accggatcta caaaaaaata aaaagccaga ctggtggtat gtatctgtgt
     3901 cccagctaat tgggagggtg agttgggagg attgtttgag cctaggagag ggaggttgca
     3961 gtgagccgtg attgcaccac tgcactccag cctgggcaac agagcaagac cctgtcttgg
     4021 agaaaccaga attttggaag agcaaatggg gctgagtgca gtggctcatg cctgtaatcc
     4081 cagcacttag ggtgccaatg tggattacct gagcccagga gtttgagacc agcctgggta
     4141 acagtgagac ccccctccct acaaaagatt ttaataatta gttgggcgta gtggtgcatg
     4201 cctgtaatcc cagctactct ggagacaggt ggaggggatt gcttgagcct gggaagctga
     4261 ggctgcagta gccatgactg caccactgca ttccagcctg gttgacagag tgacccttgt
     4321 ctccaagaaa aaaaaaaagc aaatgggatt aaggactcat ggaatgggaa ggggaagggg
     4381 agtcttacta tatgtggaat aaacttgctc agtgttgcca cagagttaca ttaccaatgt
     4441 gtagaagtca gaaactagac ttctagtttg gttcaacatt tgagggttgt tttatagcca
     4501 tcaaagtatt caagaggttg gatggcactc aaaagattct acatgtctag aatcaaactc
     4561 aaatctggca cgtaggtgct tcttagtaaa tgcttaatga atgccaccaa ctctgcaaag
     4621 tattcattga ttttgagacc ttagtcatgt tgctactaaa agtagggcat atgaccgcct
     4681 aagaaacaga acttcgagaa gtcctaacgt aaccacagta aagcagactc ccctttcagc
     4741 aacctcaaag caaaaaaaaa aaaaaaaaaa ccagttagct gctatataaa cactatacca
     4801 agcactaggc ttagaggaaa agatgtcaga ttgtactgtc actggctatg tacccatatt
     4861 ctctaagaca gggtagcatt tgtctttcaa actgcagggt ttgaaatcag tttttgtggg
     4921 tgattgcaat tttttaatga aatgcattag aacatctaag cacgtcacct acagttttgt
     4981 gatggtgcat ttttgcattg cagtatagat ctgtgcatag atatatgcat taagtgtagc
     5041 aaatcgtgat gtaaattttt tactgctggt catggtcaaa aagtaagata cgctaggaaa
     5101 ttgagtcact aggagcatgg atgtataagg caggttttat acaaccattt agattcaaca
     5161 ttaatggtag agtggcattt taccaaaaaa atgggtgtat ttgatttggg tgctgtaagg
     5221 caatttgcta ggacatggga taatcagatt acacaaaatc taggcagtga acaagtcttc
     5281 atcctggccc aggaaatagc ctattaaaaa atgctgtcca ggccaggcac agtggctcac
     5341 acctgtaatc ccaggacttc gggaggctga ggctggagga ttgcttgagt tcaagaccag
     5401 cctgagcaac acagtgaaaa cccatctcaa aaaaaaaaaa aaaaaaccaa aaaaactgtc
     5461 cagctgtgga ctttcaggat tcaggactgc tggggatcaa gcccaaggat gtttttcaga
     5521 gctctgtgat ttaagatgct cgaaaagagt gtccaagagg agtaaaggtc atgacagaat
     5581 ttactcccag gacaatctaa gttctgccac aagtacccgt ggtgtctgtt cccacaacaa
     5641 tggccctttc acaagctctt gcctcacaac ccctgcagaa gtccttcaac aaaactaata
     5701 atagactagt gaaacctact cctcacatgg gtaagagttg cagtgggcag gtgaccctcc
     5761 tccctgcccc catcctttgc ttcctcaagc tccctgccaa cctctggata catcaatggg
     5821 aaggaaacca gggaagcata gacctatagt acaacagggg tgtagtgacc actggacctg
     5881 atgaagcgaa tctgcctgaa atttaatacg cctttttatt tcccttctgt gttaaatcaa
     5941 atgatctgtt ctgcactgag ccaagcaagt tacttttaaa actggtggca ccactcattt
     6001 gggacttgga gactgctttt atccagaacc tgttaagaga acaggggatt taaatacaaa
     6061 ggaatatgaa ggtggtgtgc cttaaagaca gtctaaaatt aggttttagt ttgttacatt
     6121 attttgaaat attaaacatg aaaatgttaa atcaggatgt gtgagtttta agatgttgaa
     6181 cactgtccta catcagtgag gagggaggca ataaagtaat ttcagagtaa aacagattga
     6241 ggatgaaacc aagacagaag tgatgatttg gctttttatg atttttgctg tggaaatggc
     6301 agtcgtgtga cttttcactt gcagtttaaa tgaaagggtg aagagaagcc tacctgccct
     6361 aatggctcag ggctatatcc acctcccgga taaccctggc ccttgggact ccatcatctc
     6421 cttgaagtag cactgagaat ccaagaagag gctccgctgc tttttgcaca tgttactgag
     6481 ttacatctca ggaagatttt taagcacgag gaaggaaaat acaggcctgg ccaagcaggg
     6541 tccccttttc ggtatcatct ttgttcctaa taagcaatca aggggggtgg gtgtgttggc
     6601 tggtaaagga actactaaga ttcagaagct tgtagtcttc attattttgt tttacaggtt
     6661 aaaataaacc acttgactgg g
//
