LOCUS       NM_000952               3993 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens platelet activating factor receptor (PTAFR),
            transcript variant 3, mRNA.
ACCESSION   NM_000952
VERSION     NM_000952.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Deuster,E., Hysenaj,I., Kahaly,M., Schmoeckel,E., Mayr,D.,
            Beyer,S., Kolben,T., Hester,A., Kraus,F., Chelariu-Raicu,A.,
            Burges,A., Mahner,S., Jeschke,U., Trillsch,F. and Czogalla,B.
  TITLE     The Platelet-Activating Factor Receptor's Association with the
            Outcome of Ovarian Cancer Patients and Its Experimental Inhibition
            by Rupatadine
  JOURNAL   Cells 10 (9), 2337 (2021)
   PUBMED   34571986
  REMARK    GeneRIF: The Platelet-Activating Factor Receptor's Association with
            the Outcome of Ovarian Cancer Patients and Its Experimental
            Inhibition by Rupatadine.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Midgley,A., Barakat,D., Braitch,M., Nichols,C., Nebozhyn,M.,
            Edwards,L.J., Fox,S.C., Gran,B., Robins,R.A., Showe,L.C. and
            Constantinescu,C.S.
  TITLE     PAF-R on activated T cells: Role in the IL-23/Th17 pathway and
            relevance to multiple sclerosis
  JOURNAL   Immunobiology 226 (1), 152023 (2021)
   PUBMED   33278709
  REMARK    GeneRIF: PAF-R on activated T cells: Role in the IL-23/Th17 pathway
            and relevance to multiple sclerosis.
REFERENCE   3  (bases 1 to 3993)
  AUTHORS   Liu,J., Chen,X., Zhou,J., Ye,L., Yang,D. and Song,Y.
  TITLE     Particulate matter exposure promotes Pseudomonas aeruginosa
            invasion into airway epithelia by upregulating PAFR via the
            ROS-mediated PI3K pathway
  JOURNAL   Hum Cell 33 (4), 963-973 (2020)
   PUBMED   32627147
  REMARK    GeneRIF: Particulate matter exposure promotes Pseudomonas
            aeruginosa invasion into airway epithelia by upregulating PAFR via
            the ROS-mediated PI3K pathway.
REFERENCE   4  (bases 1 to 3993)
  AUTHORS   Liu,L., Fahy,K.E., Awoyemi,A.A., Thapa,P., Kelly,L.E., Chen,J.,
            Bihl,J.C., Cool,D.R., Chen,Y., Rapp,C.M., Johnson,R.M. and
            Travers,J.B.
  TITLE     Thermal Burn Injury Generates Bioactive Microvesicles: Evidence for
            a Novel Transport Mechanism for the Lipid Mediator
            Platelet-Activating Factor (PAF) That Involves Subcellular
            Particles and the PAF Receptor
  JOURNAL   J Immunol 205 (1), 193-201 (2020)
   PUBMED   32434939
  REMARK    GeneRIF: Thermal Burn Injury Generates Bioactive Microvesicles:
            Evidence for a Novel Transport Mechanism for the Lipid Mediator
            Platelet-Activating Factor (PAF) That Involves Subcellular
            Particles and the PAF Receptor.
REFERENCE   5  (bases 1 to 3993)
  AUTHORS   Cao,C., Tan,Q., Xu,C., He,L., Yang,L., Zhou,Y., Zhou,Y., Qiao,A.,
            Lu,M., Yi,C., Han,G.W., Wang,X., Li,X., Yang,H., Rao,Z., Jiang,H.,
            Zhao,Y., Liu,J., Stevens,R.C., Zhao,Q., Zhang,X.C. and Wu,B.
  TITLE     Structural basis for signal recognition and transduction by
            platelet-activating-factor receptor
  JOURNAL   Nat Struct Mol Biol 25 (6), 488-495 (2018)
   PUBMED   29808000
  REMARK    GeneRIF: The PAF Receptor (PAFR) structures, combined with
            single-molecule FRET and cell-based functional assays, suggest that
            the conformational change in the helical bundle is ligand dependent
            and plays a critical role in PAFR activation, thus greatly
            extending knowledge about signaling by G-protein-coupled receptors
REFERENCE   6  (bases 1 to 3993)
  AUTHORS   Mutoh,H., Bito,H., Minami,M., Nakamura,M., Honda,Z., Izumi,T.,
            Nakata,R., Kurachi,Y., Terano,A. and Shimizu,T.
  TITLE     Two different promoters direct expression of two distinct forms of
            mRNAs of human platelet-activating factor receptor
  JOURNAL   FEBS Lett 322 (2), 129-134 (1993)
   PUBMED   8387031
REFERENCE   7  (bases 1 to 3993)
  AUTHORS   Sugimoto,T., Tsuchimochi,H., McGregor,C.G., Mutoh,H., Shimizu,T.
            and Kurachi,Y.
  TITLE     Molecular cloning and characterization of the platelet-activating
            factor receptor gene expressed in the human heart
  JOURNAL   Biochem Biophys Res Commun 189 (2), 617-624 (1992)
   PUBMED   1281995
REFERENCE   8  (bases 1 to 3993)
  AUTHORS   Seyfried,C.E., Schweickart,V.L., Godiska,R. and Gray,P.W.
  TITLE     The human platelet-activating factor receptor gene (PTAFR) contains
            no introns and maps to chromosome 1
  JOURNAL   Genomics 13 (3), 832-834 (1992)
   PUBMED   1322356
REFERENCE   9  (bases 1 to 3993)
  AUTHORS   Kunz,D., Gerard,N.P. and Gerard,C.
  TITLE     The human leukocyte platelet-activating factor receptor. cDNA
            cloning, cell surface expression, and construction of a novel
            epitope-bearing analog
  JOURNAL   J Biol Chem 267 (13), 9101-9106 (1992)
   PUBMED   1374385
REFERENCE   10 (bases 1 to 3993)
  AUTHORS   Shukla,S.D.
  TITLE     Platelet-activating factor receptor and signal transduction
            mechanisms
  JOURNAL   FASEB J 6 (6), 2296-2301 (1992)
   PUBMED   1312046
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA923972.1, BC063000.1 and
            AL137792.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000952.4.
            
            Summary: This gene encodes a seven-transmembrane G-protein-coupled
            receptor for platelet-activating factor (PAF) that localizes to
            lipid rafts and/or caveolae in the cell membrane. PAF
            (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a
            phospholipid that plays a significant role in oncogenic
            transformation, tumor growth, angiogenesis, metastasis, and
            pro-inflammatory processes. Binding of PAF to the PAF-receptor
            (PAFR) stimulates numerous signal transduction pathways including
            phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs),
            and the phosphatidylinositol-calcium second messenger system.
            Following PAFR activation, cells become rapidly desensitized and
            this refractory state is dependent on PAFR phosphorylation,
            internalization, and down-regulation. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (3) lacks the 5' exon, but has an
            alternate 5' segment, as compared to variant 1. Variants 1-4 encode
            the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK290240.1, SRR3476690.162927.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373857.8/ ENSP00000362965.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-321               DA923972.1         255-575
            322-1832            BC063000.1         312-1822
            1833-3993           AL137792.11        26691-28851
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..3993
                     /gene="PTAFR"
                     /gene_synonym="PAFR"
                     /note="platelet activating factor receptor"
                     /db_xref="GeneID:5724"
                     /db_xref="HGNC:HGNC:9582"
                     /db_xref="MIM:173393"
     exon            1..99
                     /gene="PTAFR"
                     /gene_synonym="PAFR"
                     /inference="alignment:Splign:2.1.0"
     exon            100..3993
                     /gene="PTAFR"
                     /gene_synonym="PAFR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3969..3974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PTAFR"
                     /gene_synonym="PAFR"
                     /note="hexamer: AATAAA"
     polyA_site      3993
                     /gene="PTAFR"
                     /gene_synonym="PAFR"
                     /note="major polyA site"
ORIGIN      
        1 acttctgcaa ttgccagaca gcatagaggc tgaggctggg gccaggaccc agacagagac
       61 acacggtcac tgcagctgaa gccgctgccc ctgctacagg caccaccagg accagctgat
      121 cattccagcc cacagcaatg gagccacatg actcctccca catggactct gagttccgat
      181 acactctctt cccgattgtt tacagcatca tctttgtgct cggggtcatt gctaatggct
      241 acgtgctgtg ggtctttgcc cgcctgtacc cttgcaagaa attcaatgag ataaagatct
      301 tcatggtgaa cctcaccatg gcggacatgc tcttcttgat caccctgcca ctttggattg
      361 tctactacca aaaccagggc aactggatac tccccaaatt cctgtgcaac gtggctggct
      421 gccttttctt catcaacacc tactgctctg tggccttcct gggcgtcatc acttataacc
      481 gcttccaggc agtaactcgg cccatcaaga ctgctcaggc caacacccgc aagcgtggca
      541 tctctttgtc cttggtcatc tgggtggcca ttgtgggagc tgcatcctac ttcctcatcc
      601 tggactccac caacacagtg cccgacagtg ctggctcagg caacgtcact cgctgctttg
      661 agcattacga gaagggcagc gtgccagtcc tcatcatcca catcttcatc gtgttcagct
      721 tcttcctggt cttcctcatc atcctcttct gcaacctggt catcatccgt accttgctca
      781 tgcagccggt gcagcagcag cgcaacgctg aagtcaagcg ccgggcgctg tggatggtgt
      841 gcacggtctt ggcggtgttc atcatctgct tcgtgcccca ccacgtggtg cagctgccct
      901 ggacccttgc tgagctgggc ttccaggaca gcaaattcca ccaggccatt aatgatgcac
      961 atcaggtcac cctctgcctc cttagcacca actgtgtctt agaccctgtt atctactgtt
     1021 tcctcaccaa gaagttccgc aagcacctca ccgaaaagtt ctacagcatg cgcagtagcc
     1081 ggaaatgctc ccgggccacc acggatacgg tcactgaagt ggttgtgcca ttcaaccaga
     1141 tccctggcaa ttccctcaaa aattagtccc tgcttccagg cctgaagtct tctcctccat
     1201 gaacatcatg gactgagctg ggggaagaag ggatatctac tgtggtctgg gcaccacctc
     1261 tgtgggcact ggtgggccat tagatttgga ggctacctca cctgggcagg gatgatggca
     1321 gagccaggct gttggaaaat ccagaactca aatgagcccc ttcatccgcc tgtgggcgca
     1381 tactacagta actgtgactg atgactttat cctgagtccc ttaatcttat ggggccggaa
     1441 ggaatgtcag ggccaggtgc agaccttggg ggaagacttt aaaccaccta gttctccccg
     1501 atggggcatc ggtctaaagc tttgggggag tgggcgcagt ggctcacacc tgtaatccca
     1561 gcactttggg aggccgaggt gggcagatca tgggtcaaga gatcgagacc atcctggcca
     1621 acattgtaaa accccatctc tactaaaaca tacaaaaatt agccgggcat ggtggcacac
     1681 gcctgtagtc ccagctactc aggaggctga ggcaggagaa tcgcttgaac ctgggaggca
     1741 gaggttgcag tgaacctaga ttgcaccatt gcactctagc ctggcaacag agcgagattc
     1801 catctcaaaa aaaaaaaaaa aaaaaaaaaa aaagctttgg gcaactactc tcaagtgata
     1861 gaggtagact gggttaggaa aggacactgc cctcactgta tggcactacc ttggggcaga
     1921 cttttgagct aagatgttgc catcctctct ccaccctccg gcacaaggtc tttgtgacct
     1981 ttctcagggg aaggggcttg atggaacctt ctctgactca tctggcaacc tgtccctaat
     2041 ggtgaccaaa gaagataaaa catgaacaga ccaaaagcta ggacaagagc ttgcttccca
     2101 agacagacgt gtctgtctgc aaaggccttg cctctagtcc agtcagctct gggggagtgg
     2161 gaaggagatg cccacctcct ctttctgtct caacaatagt ccctaattaa gtaccttctg
     2221 tggggcagcc acccactgag ggtgggcaaa ggccccttga aagcaaaatt ggggctgggc
     2281 tcagtggctc atgcctataa tcccagcact ttgggaggcc aaggcaggca gatcacctga
     2341 ggtccggagt tcgagaccag cctggccaac gtggtgaaac gccatctcca ctaagaatac
     2401 aaaaatatgg ctcatgcctg taatcccagt actttgggag gctgaggtgg gccgatcatt
     2461 tgaagttagg agtttgagac cagcctgacc aatatggtaa aaccccgtct ctactaaaaa
     2521 tacaaaaaaa ttagcctggc gtggtggcgc atgcctgtag tcccataggc tgtagctact
     2581 gggaggaggc ggagcttgca gtgagccgag atcacgccac tgctctccag cctgggcgac
     2641 agagtgagac tctgtgtcaa aaacaaacaa acaaacaaaa aaggcaaaat tggagctgtc
     2701 caggccagga caccccgatg tccaggggct tccatacgaa caggcacatg ggctgggaag
     2761 tacatgaggc ccctgggtag aagggtctgt cagttctctc ctcccttgcc ctctgggagg
     2821 gtcctcctaa catagcttcc aggaggtggg aggagcagtt actgtcagca ggtgtcagcc
     2881 aggtgtcagc ttctcctggg gatctctaga tgtctgcttg tgatttttgg caagtatatg
     2941 caaatgagcc tcctctcctg ccctgagaca agtatctgca gtgtgaacct ggcagcctca
     3001 gacccaaggg gctcagagga aacttctctg gtttctagag ctctgtgctc cttcagagaa
     3061 gtcttccttc cttccagtca gtgtccctgt gaagctggga tactcatttc ctgtgtaccg
     3121 ggcaaacacc ggattgctga ttttgagaaa tgcctctcga tggacctgta acctgctgga
     3181 gtctgggatg gtagctgtgg gctggacttg gctgatggga tgacccggtg gctagtgcag
     3241 catcacacaa gcctggttca agtcttggct gtgtcatttc ctgctgaggg accaggcact
     3301 gaatttccta cctcttaggg tcattaccta tgaggttaaa gctacctcat gggattgtta
     3361 tacgccacta atgttgaggc agacacctct tggcagggtg actgctcatc ttagaccctc
     3421 cccttttctg cgaatttggg ccccttgatc ctctgatggg agctgaaagg atgagaggtg
     3481 ggcatctaga tttagggagg ctgttcaggc tttgcaggtc ccttacctga acacatagaa
     3541 accctggagc tgtgactgtg tccatgtgtg tgtgtttgtc tgtgtgtgtt gcgggggatg
     3601 ggcacctgca tgaatgtggt agagaaaatg gctctgctca gagggaagat acgcatagca
     3661 aggcagggac cagaggaatc acaggcgcct ggagagcagc cgggcaccgc ctccagggac
     3721 ctgccggctt ccctcagtcc tccaggggcc cagcactctt cctttaggcc ctgtgagcgt
     3781 cccttgtcag gatacattct ctcattttgc tgaagctgat ttgattgggt gtctgtttct
     3841 cgcagccaaa agagctctga atgaggaaag tgcttctgtg ctaactcccc gcgtctcctg
     3901 aatttcagtc attcatgtac ccgcctcgaa atttttgcaa tatctgtgta ccaactgtcc
     3961 atttacttaa taaagaagtt ttctttaaat taa
//
