LOCUS       NM_020232               1070 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens proteasome assembly chaperone 2 (PSMG2), transcript
            variant 1, mRNA.
ACCESSION   NM_020232
VERSION     NM_020232.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Viard,J., Loe-Mie,Y., Daudin,R., Khelfaoui,M., Plancon,C.,
            Boland,A., Tejedor,F., Huganir,R.L., Kim,E., Kinoshita,M., Liu,G.,
            Haucke,V., Moncion,T., Yu,E., Hindie,V., Blehaut,H., Mircher,C.,
            Herault,Y., Deleuze,J.F., Rain,J.C., Simonneau,M. and
            Lepagnol-Bestel,A.M.
  TITLE     Chr21 protein-protein interactions: enrichment in proteins involved
            in intellectual disability, autism, and late-onset Alzheimer's
            disease
  JOURNAL   Life Sci Alliance 5 (12), e202101205 (2022)
   PUBMED   35914814
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1070)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1070)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1070)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   6  (bases 1 to 1070)
  AUTHORS   Todd,J.A., Walker,N.M., Cooper,J.D., Smyth,D.J., Downes,K.,
            Plagnol,V., Bailey,R., Nejentsev,S., Field,S.F., Payne,F.,
            Lowe,C.E., Szeszko,J.S., Hafler,J.P., Zeitels,L., Yang,J.H.,
            Vella,A., Nutland,S., Stevens,H.E., Schuilenburg,H., Coleman,G.,
            Maisuria,M., Meadows,W., Smink,L.J., Healy,B., Burren,O.S.,
            Lam,A.A., Ovington,N.R., Allen,J., Adlem,E., Leung,H.T.,
            Wallace,C., Howson,J.M., Guja,C., Ionescu-Tirgoviste,C.,
            Simmonds,M.J., Heward,J.M., Gough,S.C., Dunger,D.B., Wicker,L.S.
            and Clayton,D.G.
  CONSRTM   Genetics of Type 1 Diabetes in Finland; Wellcome Trust Case Control
            Consortium
  TITLE     Robust associations of four new chromosome regions from genome-wide
            analyses of type 1 diabetes
  JOURNAL   Nat Genet 39 (7), 857-864 (2007)
   PUBMED   17554260
REFERENCE   7  (bases 1 to 1070)
  AUTHORS   Hirano,Y., Hayashi,H., Iemura,S., Hendil,K.B., Niwa,S.,
            Kishimoto,T., Kasahara,M., Natsume,T., Tanaka,K. and Murata,S.
  TITLE     Cooperation of multiple chaperones required for the assembly of
            mammalian 20S proteasomes
  JOURNAL   Mol Cell 24 (6), 977-984 (2006)
   PUBMED   17189198
REFERENCE   8  (bases 1 to 1070)
  AUTHORS   Hirano,Y., Hendil,K.B., Yashiroda,H., Iemura,S., Nagane,R.,
            Hioki,Y., Natsume,T., Tanaka,K. and Murata,S.
  TITLE     A heterodimeric complex that promotes the assembly of mammalian 20S
            proteasomes
  JOURNAL   Nature 437 (7063), 1381-1385 (2005)
   PUBMED   16251969
  REMARK    GeneRIF: two chaperones, designated proteasome assembling
            chaperone-1 (PAC1) and PAC2, form a heterodimer and are involved in
            the maturation of mammalian 20S proteasomes
REFERENCE   9  (bases 1 to 1070)
  AUTHORS   Bahar,R., O-Wang,J., Kawamura,K., Seimiya,M., Wang,Y., Hatano,M.,
            Okada,S., Tokuhisa,T., Watanabe,T. and Tagawa,M.
  TITLE     Growth retardation, polyploidy, and multinucleation induced by
            Clast3, a novel cell cycle-regulated protein
  JOURNAL   J Biol Chem 277 (42), 40012-40019 (2002)
   PUBMED   12147697
REFERENCE   10 (bases 1 to 1070)
  AUTHORS   Wang,Z.X., Hu,G.F., Wang,H.Y. and Wu,M.C.
  TITLE     Expression of liver cancer associated gene HCCA3
  JOURNAL   World J Gastroenterol 7 (6), 821-825 (2001)
   PUBMED   11854909
  REMARK    GeneRIF: A novel full-length cDNA was cloned and differentiated,
            which was highly expressed in liver cancer tissues. (HCCA3)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BF668035.1, AK057005.1 and BC013356.2.
            
            On Nov 23, 2018 this sequence version replaced NM_020232.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.538402.1,
                                           SRR5189655.8384.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317615.11/ ENSP00000325919.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BF668035.1         56-69
            15-1068             AK057005.1         1-1054
            1069-1070           BC013356.2         1046-1047
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.21"
     gene            1..1070
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /note="proteasome assembly chaperone 2"
                     /db_xref="GeneID:56984"
                     /db_xref="HGNC:HGNC:24929"
                     /db_xref="MIM:609702"
     exon            1..123
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..861
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hepatocellular carcinoma susceptibility protein; CD40
                     ligand-activated specific transcript 3; likely ortholog of
                     mouse CD40 ligand-activated specific transcript 3
                     (Clast3); HDCMC29P; HSPC260; x 003 protein; proteasome
                     assembling chaperone 2; PAC-2; hepatocellular
                     carcinoma-susceptibility protein 3; proteasome (prosome,
                     macropain) assembly chaperone 2; tumor necrosis factor
                     superfamily member 5-induced protein 1"
                     /codon_start=1
                     /product="proteasome assembly chaperone 2 isoform 1"
                     /protein_id="NP_064617.2"
                     /db_xref="CCDS:CCDS11862.1"
                     /db_xref="GeneID:56984"
                     /db_xref="HGNC:HGNC:24929"
                     /db_xref="MIM:609702"
                     /translation="MFVPCGESAPDLAGFTLLMPAVSVGNVGQLAMDLIISTLNMSKI
                     GYFYTDCLVPMVGNNPYATTEGNSTELSINAEVYSLPSRKLVALQLRSIFIKYKSKPF
                     CEKLLSWVKSSGCARVIVLSSSHSYQRNDLQLRSTPFRYLLTPSMQKSVQNKIKSLNW
                     EEMEKSRCIPEIDDSEFCIRIPGGGITKTLYDESCSKEIQMAVLLKFVSEGDNIPDAL
                     GLVEYLNEWLQILKPLSDDPTVSASRWKIPSSWRLLFGSGLPPALF"
     exon            124..295
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            296..354
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            355..473
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            474..647
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            648..768
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     exon            769..1070
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1047..1052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /note="hexamer: AATAAA"
     polyA_site      1070
                     /gene="PSMG2"
                     /gene_synonym="CLAST3; HCCA3; HsT1707; MDS003; PAC2;
                     PRAAS4; TNFSF5IP1"
                     /note="major polyA site"
ORIGIN      
        1 gccttcttgc tgccctcgtt cttgccaggg ccgcggttag tccctgctgg ccaccccact
       61 gcgaccatgt tcgttccctg cggggagtcg gcccccgacc ttgccggctt caccctccta
      121 atgccagcag tatctgttgg aaatgttggc cagcttgcaa tggatctgat tatttctaca
      181 ctgaatatgt ctaagattgg ttacttctat accgattgtc ttgtgccaat ggttggaaac
      241 aatccatatg cgaccacaga aggaaattca acagaactta gcataaatgc tgaagtgtat
      301 tcattgcctt caagaaagct ggtggctcta cagttaagat ccatttttat taagtataaa
      361 tcaaagccat tctgtgaaaa actgctttcc tgggtgaaaa gcagtggctg tgccagagtc
      421 attgttcttt caagcagtca ttcatatcag cgtaatgatc tgcagcttcg tagtactccc
      481 ttccggtacc tacttacacc ttccatgcaa aaaagtgttc aaaataaaat aaagagcctt
      541 aactgggaag aaatggaaaa aagccggtgc attcctgaaa tagatgattc cgagttttgt
      601 atccgcattc cgggaggagg tatcacaaaa acactctatg atgaaagctg ttctaaagaa
      661 atccaaatgg cagttctgct gaaatttgtt tcagaagggg acaacatccc agatgcatta
      721 ggtcttgttg agtatcttaa tgagtggctt cagatactca aaccacttag cgatgacccc
      781 acagtatctg cctcacggtg gaaaatacca agttcttgga gattactctt tggcagtggt
      841 cttccccctg cacttttctg atctaatttc tgttttatac cttataccca aaacacttac
      901 taccaacaca gctgttaaac attctataca aaaaaattgt atgatctggt attaggaaat
      961 tactttcaca gtaaatatca aagaaaaaag attaagggtc tctttgccat gcttttcatc
     1021 atatgcacca aatgtaaatt ttgtacaata aaattttatt tcctaagtaa
//
