LOCUS       NM_006814               8154 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens proteasome inhibitor subunit 1 (PSMF1), transcript
            variant 1, mRNA.
ACCESSION   NM_006814
VERSION     NM_006814.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Yang,S., Tang,D., Zhao,Y.C., Liu,H., Luo,S., Stinchcombe,T.E.,
            Glass,C., Su,L., Shen,S., Christiani,D.C., Wang,Q. and Wei,Q.
  TITLE     Potentially functional variants of ERAP1, PSMF1 and NCF2 in the
            MHC-I-related pathway predict non-small cell lung cancer survival
  JOURNAL   Cancer Immunol Immunother 70 (10), 2819-2833 (2021)
   PUBMED   33651148
  REMARK    GeneRIF: Potentially functional variants of ERAP1, PSMF1 and NCF2
            in the MHC-I-related pathway predict non-small cell lung cancer
            survival.
REFERENCE   3  (bases 1 to 8154)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 8154)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 8154)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 8154)
  AUTHORS   McCutchen-Maloney,S.L., Matsuda,K., Shimbara,N., Binns,D.D.,
            Tanaka,K., Slaughter,C.A. and DeMartino,G.N.
  TITLE     cDNA cloning, expression, and functional characterization of PI31,
            a proline-rich inhibitor of the proteasome
  JOURNAL   J Biol Chem 275 (24), 18557-18565 (2000)
   PUBMED   10764772
REFERENCE   7  (bases 1 to 8154)
  AUTHORS   Tanahashi,N., Kawahara,H., Murakami,Y. and Tanaka,K.
  TITLE     The proteasome-dependent proteolytic system
  JOURNAL   Mol Biol Rep 26 (1-2), 3-9 (1999)
   PUBMED   10363639
  REMARK    Review article
REFERENCE   8  (bases 1 to 8154)
  AUTHORS   Simon,J.H., Gaddis,N.C., Fouchier,R.A. and Malim,M.H.
  TITLE     Evidence for a newly discovered cellular anti-HIV-1 phenotype
  JOURNAL   Nat Med 4 (12), 1397-1400 (1998)
   PUBMED   9846577
REFERENCE   9  (bases 1 to 8154)
  AUTHORS   Madani,N. and Kabat,D.
  TITLE     An endogenous inhibitor of human immunodeficiency virus in human
            lymphocytes is overcome by the viral Vif protein
  JOURNAL   J Virol 72 (12), 10251-10255 (1998)
   PUBMED   9811770
REFERENCE   10 (bases 1 to 8154)
  AUTHORS   Seeger,M., Ferrell,K., Frank,R. and Dubiel,W.
  TITLE     HIV-1 tat inhibits the 20 S proteasome and its 11 S
            regulator-mediated activation
  JOURNAL   J Biol Chem 272 (13), 8145-8148 (1997)
   PUBMED   9079628
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC398668.1, AL031665.19,
            BC126127.1, D88378.1 and AA699495.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006814.4.
            
            Summary: The 26S proteasome is a multicatalytic proteinase complex
            with a highly ordered structure composed of 2 complexes, a 20S core
            and a 19S regulator. The 20S core is composed of 4 rings of 28
            non-identical subunits; 2 rings are composed of 7 alpha subunits
            and 2 rings are composed of 7 beta subunits. The 19S regulator is
            composed of a base, which contains 6 ATPase subunits and 2
            non-ATPase subunits, and a lid, which contains up to 10 non-ATPase
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            protein that inhibits the activation of the proteasome by the 11S
            and 19S regulators. Alternative transcript variants have been
            identified for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1. Both
            variants 1 and 2 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.109141.1,
                                           SRR1803614.103298.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335877.11/ ENSP00000338039.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                DC398668.1         10-93
            85-279              AL031665.19        5995-6189
            280-508             BC126127.1         166-394
            509-1103            D88378.1           463-1057
            1104-7998           AL031665.19        52506-59400
            7999-8154           AA699495.1         12-167              c
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..8154
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="proteasome inhibitor subunit 1"
                     /db_xref="GeneID:9491"
                     /db_xref="HGNC:HGNC:9571"
                     /db_xref="MIM:617858"
     exon            1..301
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..988
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proteasome inhibitor PI31 subunit; proteasome (prosome,
                     macropain) inhibitor subunit 1 (PI31)"
                     /codon_start=1
                     /product="proteasome inhibitor PI31 subunit isoform 1"
                     /protein_id="NP_006805.2"
                     /db_xref="CCDS:CCDS13010.1"
                     /db_xref="GeneID:9491"
                     /db_xref="HGNC:HGNC:9571"
                     /db_xref="MIM:617858"
                     /translation="MAGLEVLFASAAPAITCRQDALVCFLHWEVVTHGYFGLGVGDQP
                     GPNDKKSELLPAGWNNNKDLYVLRYEYKDGSRKLLVKAITVESSMILNVLEYGSQQVA
                     DLTLNLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPP
                     ATAREVDPLRIPPHHPHTSRQPPWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFP
                     RALIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNPDHLPPPGYDDMYL"
     exon            302..454
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            455..537
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            538..723
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            724..777
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            778..936
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            937..8154
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1361..1366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATACA"
     polyA_site      1384
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="major polyA site"
     regulatory      3208..3213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATAAA"
     polyA_site      3229
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     regulatory      4262..4267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATAAA"
     polyA_site      4286
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     regulatory      4807..4812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: CATAAA"
     polyA_site      4829
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     polyA_site      6182
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     regulatory      8127..8132
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: ATTAAA"
     polyA_site      8154
                     /gene="PSMF1"
                     /gene_synonym="PI31"
ORIGIN      
        1 ggcttccccg ccccgccccg tccccgggcg tctccatttt ggtctcaggt gtggactcgg
       61 caagaaccag cgcaagaggg aagcagagtt atagctaccc cggccgcgga gccggctcac
      121 tgcactaccc ccgccccctt ctttcctcca gacgccgaag tcgcgggcgc tcatggcggg
      181 cctggaggta ctgttcgcat cggcagcgcc ggccatcacc tgcaggcagg acgcgctcgt
      241 ctgcttcttg cattgggaag tggtgacaca cggttacttc ggcttgggtg tcggtgacca
      301 gccgggtccc aatgataaga agtcagaact gctgccagct gggtggaaca acaataaaga
      361 cctgtatgtc ctccggtatg agtataagga tgggtccaga aagctccttg tgaaagccat
      421 caccgtggag agcagcatga tcctcaatgt gctggaatat ggctcacagc aagtggcaga
      481 cttgaccctg aacttggatg attatatcga tgcagaacac ctgggtgact tccacaggac
      541 ctacaagaac agtgaggagc ttcggtctcg tattgtgtct ggaatcatca cacctatcca
      601 tgagcagtgg gaaaaggcta atgtaagcag tccccaccgg gagttccccc ctgctaccgc
      661 cagagaggtg gacccactcc ggattcctcc acaccaccca cacaccagtc ggcagcctcc
      721 ctggtgtgat cccctgggcc cgtttgttgt cgggggagaa gacttagacc cttttgggcc
      781 tcggagaggt ggcatgattg tggatcccct gagatctggc ttcccaagag cacttattga
      841 cccttcctca ggcctcccga accgacttcc tccaggcgct gtgcccccag gagctcgctt
      901 tgaccccttt ggacccattg ggaccagccc acccggacct aacccagacc atctcccccc
      961 gccgggctac gatgacatgt acctgtgaag gcctcaagaa tgtaacatcc caggcttccc
     1021 tccattctcc tggagctgcc accgctgtcc ccatcagcaa ccatgttctt gcaggctggg
     1081 ggcaagggat tctgctcatg tgtttgcaga ccggctggga tagcctcccc accccttatc
     1141 agagccaaga cacctgctgc agctctccac ctagctgcag atagctccca aagagaaatc
     1201 agtgtgtctc tttcaccatc agctcctccc ctttcaccac cagctcctct ccacttccca
     1261 agggagactc cggcaacctt cagcaacata tatcctcgac cagatgcagt gctataagaa
     1321 cagaacgcat tttggatgtt attattaaga accaaatgtc aatacagaat tcatgttgcc
     1381 ggtttcccac ttttcttttt acattaatgc atagctgctt ccatttatga gactttagag
     1441 tttgagtttc tgtagggctg aatgactctt tttcctgccc agggcccatt cttgcttctc
     1501 aggcaccttc cgtttattaa ttgccattgc tcctgacatc actaagatgg gtccccttct
     1561 ggctgcatga atggaaatga gtgactggaa atcccatagg ccacaagaat gactttcaca
     1621 agggcaggaa cattgtggaa agactgcatc attctgatga ggcaaaatcc tccagctatt
     1681 cctgtctggg ccagttttgt aggtccatct gtgcatgggc agcagtagtc aaaaagccaa
     1741 ggaaaaaaca gagcagacct gaaggctaat cttatttttg ccactaactt agtgaatgac
     1801 cctaagcaag ttccttctcc tcttagggcc ttgtgccaag cctatgaaat tggaggtggc
     1861 tttcctgctc taaagcattt tgatgtctca ttctgtgttt ggtaacccct ataaactggg
     1921 gcagaggaaa agaatgatgg ttcaaggcca tacttccctt gaaccttgtg tggttcttgc
     1981 ctaactctgt ggtttttgga ccccatgggg cccagacaga gcacaggagc atgggctgcc
     2041 tctgagtgtg gtgttgaact tcgggaggag cagggagccc tgcaccttgt gtcctggccc
     2101 acctgacctt tggtgttctc cggatccttt tcagcccgag gcctgacaga cgcgggcagt
     2161 gatgagccct gttctggagt ggaaagagca cgatagagca ccaggctaag aggcacgaga
     2221 tcaaggcggt agtcacttcc gctctgcagc tagcatttca accatatgtg gatcctttca
     2281 tttctcagct ccctggattc cttcccctaa attaggacct attatttacc tgtaggtaag
     2341 caagctactg tagctcttct gaggtatctg ccaggctgtt ttctgtagcc tcagattgcc
     2401 tatctgctta gcctgagaac aggtagatga aaactaaact gatgcctagg cccagggtca
     2461 gtctcagatg gaagctgggc ctgggtgggg aggctagcat gcgtggctcc ctgggtattt
     2521 ctgtcagtcc ccatggcaag cagtgattta gtaaaacacc ccagagtcag ggaagccaac
     2581 caccttgaaa cctttagaac atctctgctt tggagaaaga cccagagatc aggcagaggt
     2641 gcagattcaa tcattactca taacctttga gagatggcaa atgggaggag tgttagtctt
     2701 tgttttggaa ttggaccatt cttgcaacca aaaggactta gagcagtttg ctcataaaga
     2761 catcctttat tataaaagga agtatttaaa ggatgataga gaccatcaga tagaagcagg
     2821 gaaggtagat aacttttagg accttgatgt gaggaaaaga taaaatttaa acaataaatt
     2881 tgccacttag attttctagc agcaaagtcc gaaaagatag ttatatacaa aattctgttt
     2941 tctgaaaaca aaattgtgat tcaccttcag aattggccat tttttttgtg agtttccttg
     3001 catcaaggac actgagaaac acagtcattg tcttaggtgt tctatgggag gaagtgaata
     3061 gagcctttag gaacttcctg gtcaagctta tggtgcttat tttgatctgg gccacttccc
     3121 tccttccagt catgagtaat catcaaggag caagttggag tgtttcaggt gtatattttg
     3181 tagaacccaa aagattggag ccttaacaat aaacatcagc actaagtgac ctgttcctcc
     3241 ttttttaaat agcagcttta ttgagatata atttacatac ccataattta catacctgtt
     3301 taaagtatac aattcagtgg atttagtatg ttcacagtgt tgcacatcca ccaccatttc
     3361 taattcctga acattttcac cacctcaaaa agaaaccaca cacccattgg catgactccc
     3421 cattcccttg ctcccagccc caggcaacca ctaacatctg tctctaaaga tttttttttt
     3481 ttttctggac agttctcata agtggaatca ctcagtatta tggccttttg tgtctggctt
     3541 ctttcatttc atgtgatgtt ttcaagattc attcatgctg tagcatgtat ccatggtatg
     3601 gatatatcac attttgctta tccttttatc agttgatgga cagttgggtt gtttctacaa
     3661 aaaggctatt atgagtaatg cagccaagaa catttgtgta ccagtttttg agttttcagt
     3721 tgtttgagtt tcagatgttt tcagttcatt tgggtatata ccaaggagtg gaattgctgg
     3781 ttcacatggt agctctatat tctactttgt gaggaattgt cagactgttt tccacggaag
     3841 ctgcaccatt ttacatttcc actggtaatt tatgagggtt ccagtttccc cacatcttca
     3901 ccaactcttg ttatttttca tgcttatggc catcctagtt agcgtgaagt agcatctcat
     3961 tgtggtcttc tcttccattt tttaaaaaag aattttgacc tacaccaagc aaggcagaga
     4021 ccagagtcag tcccccttgg ggagcaatct gtggttttga taatcaccac cttgggatga
     4081 acatggctgg tgagctgcag agcagttgat ttgacttttg gggatggttc taggctggat
     4141 gtgatacagg gcaggttttg tctctagaga aagttctcat gaccttcagg tctaatgaga
     4201 ttcagatcat cactttggtt tggtttgaaa tttctttgtt tatccagtga attacaagta
     4261 gaataaagat ctggttctaa attgtctttt ggtggtgaag tgccatagac catttcggac
     4321 catttctatg atgtaccagc agcccccaca tcacacctcc tcttccttgg cttgtctctt
     4381 ccccaattct tagagttagc agtgtttctt actgcattca tctcctcagt cataagtgtc
     4441 tgtcaacctc cgttgtgaag tcatgagtct cttgagactg aaactccaag aatgtattgt
     4501 gctcacagtg gcgatggtgt tcctgtgctg gatggtcagt ggttgttgac atgtagttag
     4561 aaaataggta gcaagttcca cgcagcctca ctctttggaa cctagtttct cctatggcct
     4621 ccctttctac tgttgttaat aacattctat taatgatcca tgcctctctt cacaggagga
     4681 atattagaat ctatggagca gttagtattc tttgtcacct aaatttgtct acttctgtac
     4741 atttccaata aggcaccacc agggcaaggg aagattcccc acatgtatat ccccagctgt
     4801 ttctgtcata aaacttgcat gtgtataaac cactattgac tttttgcacc aaaggagata
     4861 ttcaaggaca gacatccaag tccccagccc tttattgccc ttgggatacc acaaggcaca
     4921 gatacaggca gctgctgtgt ggctctggag ttcctaatcc ccagattgta gcaatgatga
     4981 tgtgatcctg ctgctctctg gaccgtaacc tttttggtta caaaaggtaa atttctgttt
     5041 actgcatctg aaccccagga ctaatgcgtc actgcattaa ccccaggaca catggaccaa
     5101 gtcactgcgg ccgtacttgg ccctgaatct gtcctggagg gtgggtcaaa aggcaagggt
     5161 ggcaggtagg atggtaggca gtgaggggtg gcaaggccta gctgactgag gacagaccca
     5221 gcaacaatga caggaggttt gaacacctca cgagtggtag tgcccatggg aggtgcgaag
     5281 tggaaggccc agcccttgct tggaggaacg tgaggctgat ggtgcagtgc aatagaggtc
     5341 actggatgcc catttgaaca gctgcgttcc agatgagcca cctaaggtgg atgttgtgag
     5401 agtcacagtg ggtttagagg gacaaggtag tgagaggatg ctgctgacgc attagtcctg
     5461 ggattcagct gcagtaaaca gaaatttacc ttttgtaacc aaaaaggtaa tggtccagag
     5521 agcagcagga tcacatcatc attgctacaa tctggggatt aggaactcca gagccacaca
     5581 gcagctgcct atatctgcac caaaaagtag atgtccttca cactgccatc tggacaccac
     5641 tcagctagct catccaagtt caaggctcaa ttgaatgcgt ctggttgttg gaacctacat
     5701 ggcctctcaa atggtactgg catggaatat tggggagatg tctttattcc ctcttcccac
     5761 ctttcctgga aggtgcactg ggacagaggt ctagtgagcc aatcttctgt gttgtctacg
     5821 ccatggacca tgatataaat gggtaccaca gccttgcatg cctgagccta accatctcct
     5881 gagatgcttg ctctaagtac acattagtac atgctgggcc ccaatctaga tctcaaggga
     5941 acggccttca gatccatttt tgacaagttc cttttgtgag tcctatgatc atgcaagttt
     6001 gagaactgag aagggagggg aagacctacc tccttgttac tcagaccacc agcaacagca
     6061 gcatcacctg ggaacctgtt acacatgcag aatctcaagc cccaccccag acattgaatg
     6121 agaatctgca ttttaacaag ttctccatga gatttgtgta aacttaaagt gagagaagct
     6181 cacatggact atgtcttttt cctttacgtg aatatcgagt acaaagaatt gagccagtca
     6241 gtcctcttgg ttaatggcac attatgatta atgcatgcat gaactctgat tcgaagtttt
     6301 cgttgatttt acccccagta tccactccat tcctttgccc ccatgggtca cttagatgtg
     6361 tttcatgtta tctttaaaaa tgcagcacct ttatagcata cattatatct tcaaaccata
     6421 accataatga ggtgttatcc ccatttcctg taccagaaaa ggaaggattg ggaaggtcca
     6481 gtaatttgct cagggtcaag caggagtctc agactcagtc aagcgtggtt actcacagcc
     6541 ctccctttct cagtgtgtgg ccttcctcat ccacaaaatg agcatcaccc ctacctgcag
     6601 gattcttgca agatccaacc aaataatgtg tcttattaac agagagttgg ggctcggtgg
     6661 gtgcttgctg caattgttac taaagtcata tgggtagata agggtgggac tcaaggacaa
     6721 gagaggaacc ttggaatatc ccccagtgca ggaaacaagc atttatcagg tgcacacagc
     6781 gtgctaggct tctcctcatc gcatgtagta cccctgacaa ccccaaggta aacacagcta
     6841 gtggcattag ttgagatgct gcacctaccc cctggggtgg cagaaccgtg attggaaccc
     6901 aggtcagtgc tcttttcagt agaactgctg tacccagacc tcacatcctc tctaggtgag
     6961 gtgcatgggt ccctgggcaa aacccattcc agaagctggg aaaccacgtc aggacttgtg
     7021 tgagaaagga gtgatgcttc ataatcccca ggatggtctt aaggctcctc agtcagtcct
     7081 atttgcacag cgtgcctcat gctcctgagt acctccacct gcagccacag tgcccagagc
     7141 acctggttag gaggagcatc tgaaaccacc tgcacaacct ccattccaca gaggaagaaa
     7201 caggctcaag aaacccatat gtgatgggga gctcctaaca ggcttccctg ggccacagga
     7261 atgggctcgg gctctctgct gggctctgtc acctggctcc atgtgctgct cacctcggcc
     7321 ttctgagttt agtaggatgg cagctagtct tccctattga caggtgaaaa agcaggtcca
     7381 cagctgttga gtgactttcc caaggccaca cagaccacaa atcctggctc caagtccagg
     7441 gcgtttccag taagcactgt ccaaatcagt catggaggtc cctgagtgac aaaaatatgc
     7501 aggacccctt ctttccccct cccagttccc gcagctaggc tgagtgttaa gaggaaggtg
     7561 ccatacaggt tcagcaccct ggtcagcgcc cagcctccct aaagccgctg gaggaggcct
     7621 ttggagccag ctcaggtcag tgtctgctgc ttccttaccc aggatggccc actcttgggg
     7681 acagacaggc atcctgagag gagggggcct ggacctaggt gtcttgccac ccaatacaga
     7741 gccctgccct ctccctctcc ttcagaagga agtatgggag cagttccatc ctcaaggttt
     7801 cttggttccc aggacagaaa tttgagtgag aagcatgtga agagagggat ggcccagggg
     7861 cccttggcca tggtggcctt ggacaagttt ggctttcccc atctgtaaaa tgtagataag
     7921 gatggaccca cctccccaag atcctgccta tctgtgagag tccatgacta agtgggccat
     7981 gggctggact gagttcagtg ctcccccaac ctgccctcct cctctccccc acagagcttc
     8041 ccaatttaca tgtaaacctt gggcatcaaa gtgacagctg atagcccagg tttgcccaca
     8101 aatgtgctct gactggcgtg catgttatta aatttgataa gatgcatgat ggca
//
