LOCUS       NM_001323409            8170 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens proteasome inhibitor subunit 1 (PSMF1), transcript
            variant 5, mRNA.
ACCESSION   NM_001323409
VERSION     NM_001323409.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Yang,S., Tang,D., Zhao,Y.C., Liu,H., Luo,S., Stinchcombe,T.E.,
            Glass,C., Su,L., Shen,S., Christiani,D.C., Wang,Q. and Wei,Q.
  TITLE     Potentially functional variants of ERAP1, PSMF1 and NCF2 in the
            MHC-I-related pathway predict non-small cell lung cancer survival
  JOURNAL   Cancer Immunol Immunother 70 (10), 2819-2833 (2021)
   PUBMED   33651148
  REMARK    GeneRIF: Potentially functional variants of ERAP1, PSMF1 and NCF2
            in the MHC-I-related pathway predict non-small cell lung cancer
            survival.
REFERENCE   3  (bases 1 to 8170)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 8170)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 8170)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 8170)
  AUTHORS   McCutchen-Maloney,S.L., Matsuda,K., Shimbara,N., Binns,D.D.,
            Tanaka,K., Slaughter,C.A. and DeMartino,G.N.
  TITLE     cDNA cloning, expression, and functional characterization of PI31,
            a proline-rich inhibitor of the proteasome
  JOURNAL   J Biol Chem 275 (24), 18557-18565 (2000)
   PUBMED   10764772
REFERENCE   7  (bases 1 to 8170)
  AUTHORS   Tanahashi,N., Kawahara,H., Murakami,Y. and Tanaka,K.
  TITLE     The proteasome-dependent proteolytic system
  JOURNAL   Mol Biol Rep 26 (1-2), 3-9 (1999)
   PUBMED   10363639
  REMARK    Review article
REFERENCE   8  (bases 1 to 8170)
  AUTHORS   Simon,J.H., Gaddis,N.C., Fouchier,R.A. and Malim,M.H.
  TITLE     Evidence for a newly discovered cellular anti-HIV-1 phenotype
  JOURNAL   Nat Med 4 (12), 1397-1400 (1998)
   PUBMED   9846577
REFERENCE   9  (bases 1 to 8170)
  AUTHORS   Madani,N. and Kabat,D.
  TITLE     An endogenous inhibitor of human immunodeficiency virus in human
            lymphocytes is overcome by the viral Vif protein
  JOURNAL   J Virol 72 (12), 10251-10255 (1998)
   PUBMED   9811770
REFERENCE   10 (bases 1 to 8170)
  AUTHORS   Seeger,M., Ferrell,K., Frank,R. and Dubiel,W.
  TITLE     HIV-1 tat inhibits the 20 S proteasome and its 11 S
            regulator-mediated activation
  JOURNAL   J Biol Chem 272 (13), 8145-8148 (1997)
   PUBMED   9079628
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC398668.1, AL031665.19 and
            AA699495.1.
            
            On May 31, 2019 this sequence version replaced NM_001323409.1.
            
            Summary: The 26S proteasome is a multicatalytic proteinase complex
            with a highly ordered structure composed of 2 complexes, a 20S core
            and a 19S regulator. The 20S core is composed of 4 rings of 28
            non-identical subunits; 2 rings are composed of 7 alpha subunits
            and 2 rings are composed of 7 beta subunits. The 19S regulator is
            composed of a base, which contains 6 ATPase subunits and 2
            non-ATPase subunits, and a lid, which contains up to 10 non-ATPase
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            protein that inhibits the activation of the proteasome by the 11S
            and 19S regulators. Alternative transcript variants have been
            identified for this gene. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1037405.1,
                                           SRR7410570.659976.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                DC398668.1         10-93
            85-301              AL031665.19        5995-6211
            302-454             AL031665.19        12807-12959
            455-537             AL031665.19        14735-14817
            538-723             AL031665.19        22430-22615
            724-777             AL031665.19        50440-50493
            778-952             AL031665.19        51628-51802
            953-8014            AL031665.19        52339-59400
            8015-8170           AA699495.1         12-167              c
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..8170
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="proteasome inhibitor subunit 1"
                     /db_xref="GeneID:9491"
                     /db_xref="HGNC:HGNC:9571"
                     /db_xref="MIM:617858"
     exon            1..301
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..958
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="isoform 5 is encoded by transcript variant 5;
                     proteasome inhibitor PI31 subunit; proteasome (prosome,
                     macropain) inhibitor subunit 1 (PI31)"
                     /codon_start=1
                     /product="proteasome inhibitor PI31 subunit isoform 5"
                     /protein_id="NP_001310338.1"
                     /db_xref="GeneID:9491"
                     /db_xref="HGNC:HGNC:9571"
                     /db_xref="MIM:617858"
                     /translation="MAGLEVLFASAAPAITCRQDALVCFLHWEVVTHGYFGLGVGDQP
                     GPNDKKSELLPAGWNNNKDLYVLRYEYKDGSRKLLVKAITVESSMILNVLEYGSQQVA
                     DLTLNLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPP
                     ATAREVDPLRIPPHHPHTSRQPPWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFP
                     RALIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGYVVTQT"
     exon            302..454
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            455..537
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            538..723
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            724..777
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            778..952
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     exon            953..8170
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1377..1382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATACA"
     polyA_site      1400
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="major polyA site"
     regulatory      3224..3229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATAAA"
     polyA_site      3245
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     regulatory      4278..4283
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: AATAAA"
     polyA_site      4302
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     regulatory      4823..4828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: CATAAA"
     polyA_site      4845
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     polyA_site      6198
                     /gene="PSMF1"
                     /gene_synonym="PI31"
     regulatory      8143..8148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMF1"
                     /gene_synonym="PI31"
                     /note="hexamer: ATTAAA"
     polyA_site      8170
                     /gene="PSMF1"
                     /gene_synonym="PI31"
ORIGIN      
        1 ggcttccccg ccccgccccg tccccgggcg tctccatttt ggtctcaggt gtggactcgg
       61 caagaaccag cgcaagaggg aagcagagtt atagctaccc cggccgcgga gccggctcac
      121 tgcactaccc ccgccccctt ctttcctcca gacgccgaag tcgcgggcgc tcatggcggg
      181 cctggaggta ctgttcgcat cggcagcgcc ggccatcacc tgcaggcagg acgcgctcgt
      241 ctgcttcttg cattgggaag tggtgacaca cggttacttc ggcttgggtg tcggtgacca
      301 gccgggtccc aatgataaga agtcagaact gctgccagct gggtggaaca acaataaaga
      361 cctgtatgtc ctccggtatg agtataagga tgggtccaga aagctccttg tgaaagccat
      421 caccgtggag agcagcatga tcctcaatgt gctggaatat ggctcacagc aagtggcaga
      481 cttgaccctg aacttggatg attatatcga tgcagaacac ctgggtgact tccacaggac
      541 ctacaagaac agtgaggagc ttcggtctcg tattgtgtct ggaatcatca cacctatcca
      601 tgagcagtgg gaaaaggcta atgtaagcag tccccaccgg gagttccccc ctgctaccgc
      661 cagagaggtg gacccactcc ggattcctcc acaccaccca cacaccagtc ggcagcctcc
      721 ctggtgtgat cccctgggcc cgtttgttgt cgggggagaa gacttagacc cttttgggcc
      781 tcggagaggt ggcatgattg tggatcccct gagatctggc ttcccaagag cacttattga
      841 cccttcctca ggcctcccga accgacttcc tccaggcgct gtgcccccag gagctcgctt
      901 tgaccccttt ggacccattg ggaccagccc acccgggtac gtagtcactc agacctaacc
      961 cagaccatct ccccccgccg ggctacgatg acatgtacct gtgaaggcct caagaatgta
     1021 acatcccagg cttccctcca ttctcctgga gctgccaccg ctgtccccat cagcaaccat
     1081 gttcttgcag gctgggggca agggattctg ctcatgtgtt tgcagaccgg ctgggatagc
     1141 ctccccaccc cttatcagag ccaagacacc tgctgcagct ctccacctag ctgcagatag
     1201 ctcccaaaga gaaatcagtg tgtctctttc accatcagct cctccccttt caccaccagc
     1261 tcctctccac ttcccaaggg agactccggc aaccttcagc aacatatatc ctcgaccaga
     1321 tgcagtgcta taagaacaga acgcattttg gatgttatta ttaagaacca aatgtcaata
     1381 cagaattcat gttgccggtt tcccactttt ctttttacat taatgcatag ctgcttccat
     1441 ttatgagact ttagagtttg agtttctgta gggctgaatg actctttttc ctgcccaggg
     1501 cccattcttg cttctcaggc accttccgtt tattaattgc cattgctcct gacatcacta
     1561 agatgggtcc ccttctggct gcatgaatgg aaatgagtga ctggaaatcc cataggccac
     1621 aagaatgact ttcacaaggg caggaacatt gtggaaagac tgcatcattc tgatgaggca
     1681 aaatcctcca gctattcctg tctgggccag ttttgtaggt ccatctgtgc atgggcagca
     1741 gtagtcaaaa agccaaggaa aaaacagagc agacctgaag gctaatctta tttttgccac
     1801 taacttagtg aatgacccta agcaagttcc ttctcctctt agggccttgt gccaagccta
     1861 tgaaattgga ggtggctttc ctgctctaaa gcattttgat gtctcattct gtgtttggta
     1921 acccctataa actggggcag aggaaaagaa tgatggttca aggccatact tcccttgaac
     1981 cttgtgtggt tcttgcctaa ctctgtggtt tttggacccc atggggccca gacagagcac
     2041 aggagcatgg gctgcctctg agtgtggtgt tgaacttcgg gaggagcagg gagccctgca
     2101 ccttgtgtcc tggcccacct gacctttggt gttctccgga tccttttcag cccgaggcct
     2161 gacagacgcg ggcagtgatg agccctgttc tggagtggaa agagcacgat agagcaccag
     2221 gctaagaggc acgagatcaa ggcggtagtc acttccgctc tgcagctagc atttcaacca
     2281 tatgtggatc ctttcatttc tcagctccct ggattccttc ccctaaatta ggacctatta
     2341 tttacctgta ggtaagcaag ctactgtagc tcttctgagg tatctgccag gctgttttct
     2401 gtagcctcag attgcctatc tgcttagcct gagaacaggt agatgaaaac taaactgatg
     2461 cctaggccca gggtcagtct cagatggaag ctgggcctgg gtggggaggc tagcatgcgt
     2521 ggctccctgg gtatttctgt cagtccccat ggcaagcagt gatttagtaa aacaccccag
     2581 agtcagggaa gccaaccacc ttgaaacctt tagaacatct ctgctttgga gaaagaccca
     2641 gagatcaggc agaggtgcag attcaatcat tactcataac ctttgagaga tggcaaatgg
     2701 gaggagtgtt agtctttgtt ttggaattgg accattcttg caaccaaaag gacttagagc
     2761 agtttgctca taaagacatc ctttattata aaaggaagta tttaaaggat gatagagacc
     2821 atcagataga agcagggaag gtagataact tttaggacct tgatgtgagg aaaagataaa
     2881 atttaaacaa taaatttgcc acttagattt tctagcagca aagtccgaaa agatagttat
     2941 atacaaaatt ctgttttctg aaaacaaaat tgtgattcac cttcagaatt ggccattttt
     3001 tttgtgagtt tccttgcatc aaggacactg agaaacacag tcattgtctt aggtgttcta
     3061 tgggaggaag tgaatagagc ctttaggaac ttcctggtca agcttatggt gcttattttg
     3121 atctgggcca cttccctcct tccagtcatg agtaatcatc aaggagcaag ttggagtgtt
     3181 tcaggtgtat attttgtaga acccaaaaga ttggagcctt aacaataaac atcagcacta
     3241 agtgacctgt tcctcctttt ttaaatagca gctttattga gatataattt acatacccat
     3301 aatttacata cctgtttaaa gtatacaatt cagtggattt agtatgttca cagtgttgca
     3361 catccaccac catttctaat tcctgaacat tttcaccacc tcaaaaagaa accacacacc
     3421 cattggcatg actccccatt cccttgctcc cagccccagg caaccactaa catctgtctc
     3481 taaagatttt tttttttttt ctggacagtt ctcataagtg gaatcactca gtattatggc
     3541 cttttgtgtc tggcttcttt catttcatgt gatgttttca agattcattc atgctgtagc
     3601 atgtatccat ggtatggata tatcacattt tgcttatcct tttatcagtt gatggacagt
     3661 tgggttgttt ctacaaaaag gctattatga gtaatgcagc caagaacatt tgtgtaccag
     3721 tttttgagtt ttcagttgtt tgagtttcag atgttttcag ttcatttggg tatataccaa
     3781 ggagtggaat tgctggttca catggtagct ctatattcta ctttgtgagg aattgtcaga
     3841 ctgttttcca cggaagctgc accattttac atttccactg gtaatttatg agggttccag
     3901 tttccccaca tcttcaccaa ctcttgttat ttttcatgct tatggccatc ctagttagcg
     3961 tgaagtagca tctcattgtg gtcttctctt ccatttttta aaaaagaatt ttgacctaca
     4021 ccaagcaagg cagagaccag agtcagtccc ccttggggag caatctgtgg ttttgataat
     4081 caccaccttg ggatgaacat ggctggtgag ctgcagagca gttgatttga cttttgggga
     4141 tggttctagg ctggatgtga tacagggcag gttttgtctc tagagaaagt tctcatgacc
     4201 ttcaggtcta atgagattca gatcatcact ttggtttggt ttgaaatttc tttgtttatc
     4261 cagtgaatta caagtagaat aaagatctgg ttctaaattg tcttttggtg gtgaagtgcc
     4321 atagaccatt tcggaccatt tctatgatgt accagcagcc cccacatcac acctcctctt
     4381 ccttggcttg tctcttcccc aattcttaga gttagcagtg tttcttactg cattcatctc
     4441 ctcagtcata agtgtctgtc aacctccgtt gtgaagtcat gagtctcttg agactgaaac
     4501 tccaagaatg tattgtgctc acagtggcga tggtgttcct gtgctggatg gtcagtggtt
     4561 gttgacatgt agttagaaaa taggtagcaa gttccacgca gcctcactct ttggaaccta
     4621 gtttctccta tggcctccct ttctactgtt gttaataaca ttctattaat gatccatgcc
     4681 tctcttcaca ggaggaatat tagaatctat ggagcagtta gtattctttg tcacctaaat
     4741 ttgtctactt ctgtacattt ccaataaggc accaccaggg caagggaaga ttccccacat
     4801 gtatatcccc agctgtttct gtcataaaac ttgcatgtgt ataaaccact attgactttt
     4861 tgcaccaaag gagatattca aggacagaca tccaagtccc cagcccttta ttgcccttgg
     4921 gataccacaa ggcacagata caggcagctg ctgtgtggct ctggagttcc taatccccag
     4981 attgtagcaa tgatgatgtg atcctgctgc tctctggacc gtaacctttt tggttacaaa
     5041 aggtaaattt ctgtttactg catctgaacc ccaggactaa tgcgtcactg cattaacccc
     5101 aggacacatg gaccaagtca ctgcggccgt acttggccct gaatctgtcc tggagggtgg
     5161 gtcaaaaggc aagggtggca ggtaggatgg taggcagtga ggggtggcaa ggcctagctg
     5221 actgaggaca gacccagcaa caatgacagg aggtttgaac acctcacgag tggtagtgcc
     5281 catgggaggt gcgaagtgga aggcccagcc cttgcttgga ggaacgtgag gctgatggtg
     5341 cagtgcaata gaggtcactg gatgcccatt tgaacagctg cgttccagat gagccaccta
     5401 aggtggatgt tgtgagagtc acagtgggtt tagagggaca aggtagtgag aggatgctgc
     5461 tgacgcatta gtcctgggat tcagctgcag taaacagaaa tttacctttt gtaaccaaaa
     5521 aggtaatggt ccagagagca gcaggatcac atcatcattg ctacaatctg gggattagga
     5581 actccagagc cacacagcag ctgcctatat ctgcaccaaa aagtagatgt ccttcacact
     5641 gccatctgga caccactcag ctagctcatc caagttcaag gctcaattga atgcgtctgg
     5701 ttgttggaac ctacatggcc tctcaaatgg tactggcatg gaatattggg gagatgtctt
     5761 tattccctct tcccaccttt cctggaaggt gcactgggac agaggtctag tgagccaatc
     5821 ttctgtgttg tctacgccat ggaccatgat ataaatgggt accacagcct tgcatgcctg
     5881 agcctaacca tctcctgaga tgcttgctct aagtacacat tagtacatgc tgggccccaa
     5941 tctagatctc aagggaacgg ccttcagatc catttttgac aagttccttt tgtgagtcct
     6001 atgatcatgc aagtttgaga actgagaagg gaggggaaga cctacctcct tgttactcag
     6061 accaccagca acagcagcat cacctgggaa cctgttacac atgcagaatc tcaagcccca
     6121 ccccagacat tgaatgagaa tctgcatttt aacaagttct ccatgagatt tgtgtaaact
     6181 taaagtgaga gaagctcaca tggactatgt ctttttcctt tacgtgaata tcgagtacaa
     6241 agaattgagc cagtcagtcc tcttggttaa tggcacatta tgattaatgc atgcatgaac
     6301 tctgattcga agttttcgtt gattttaccc ccagtatcca ctccattcct ttgcccccat
     6361 gggtcactta gatgtgtttc atgttatctt taaaaatgca gcacctttat agcatacatt
     6421 atatcttcaa accataacca taatgaggtg ttatccccat ttcctgtacc agaaaaggaa
     6481 ggattgggaa ggtccagtaa tttgctcagg gtcaagcagg agtctcagac tcagtcaagc
     6541 gtggttactc acagccctcc ctttctcagt gtgtggcctt cctcatccac aaaatgagca
     6601 tcacccctac ctgcaggatt cttgcaagat ccaaccaaat aatgtgtctt attaacagag
     6661 agttggggct cggtgggtgc ttgctgcaat tgttactaaa gtcatatggg tagataaggg
     6721 tgggactcaa ggacaagaga ggaaccttgg aatatccccc agtgcaggaa acaagcattt
     6781 atcaggtgca cacagcgtgc taggcttctc ctcatcgcat gtagtacccc tgacaacccc
     6841 aaggtaaaca cagctagtgg cattagttga gatgctgcac ctaccccctg gggtggcaga
     6901 accgtgattg gaacccaggt cagtgctctt ttcagtagaa ctgctgtacc cagacctcac
     6961 atcctctcta ggtgaggtgc atgggtccct gggcaaaacc cattccagaa gctgggaaac
     7021 cacgtcagga cttgtgtgag aaaggagtga tgcttcataa tccccaggat ggtcttaagg
     7081 ctcctcagtc agtcctattt gcacagcgtg cctcatgctc ctgagtacct ccacctgcag
     7141 ccacagtgcc cagagcacct ggttaggagg agcatctgaa accacctgca caacctccat
     7201 tccacagagg aagaaacagg ctcaagaaac ccatatgtga tggggagctc ctaacaggct
     7261 tccctgggcc acaggaatgg gctcgggctc tctgctgggc tctgtcacct ggctccatgt
     7321 gctgctcacc tcggccttct gagtttagta ggatggcagc tagtcttccc tattgacagg
     7381 tgaaaaagca ggtccacagc tgttgagtga ctttcccaag gccacacaga ccacaaatcc
     7441 tggctccaag tccagggcgt ttccagtaag cactgtccaa atcagtcatg gaggtccctg
     7501 agtgacaaaa atatgcagga ccccttcttt ccccctccca gttcccgcag ctaggctgag
     7561 tgttaagagg aaggtgccat acaggttcag caccctggtc agcgcccagc ctccctaaag
     7621 ccgctggagg aggcctttgg agccagctca ggtcagtgtc tgctgcttcc ttacccagga
     7681 tggcccactc ttggggacag acaggcatcc tgagaggagg gggcctggac ctaggtgtct
     7741 tgccacccaa tacagagccc tgccctctcc ctctccttca gaaggaagta tgggagcagt
     7801 tccatcctca aggtttcttg gttcccagga cagaaatttg agtgagaagc atgtgaagag
     7861 agggatggcc caggggccct tggccatggt ggccttggac aagtttggct ttccccatct
     7921 gtaaaatgta gataaggatg gacccacctc cccaagatcc tgcctatctg tgagagtcca
     7981 tgactaagtg ggccatgggc tggactgagt tcagtgctcc cccaacctgc cctcctcctc
     8041 tcccccacag agcttcccaa tttacatgta aaccttgggc atcaaagtga cagctgatag
     8101 cccaggtttg cccacaaatg tgctctgact ggcgtgcatg ttattaaatt tgataagatg
     8161 catgatggca
//
