LOCUS       NM_006263                965 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens proteasome activator subunit 1 (PSME1), transcript
            variant 1, mRNA.
ACCESSION   NM_006263
VERSION     NM_006263.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 965)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 965)
  AUTHORS   Gu,Y., Barwick,B.G., Shanmugam,M., Hofmeister,C.C., Kaufman,J.,
            Nooka,A., Gupta,V., Dhodapkar,M., Boise,L.H. and Lonial,S.
  TITLE     Downregulation of PA28alpha induces proteasome remodeling and
            results in resistance to proteasome inhibitors in multiple myeloma
  JOURNAL   Blood Cancer J 10 (12), 125 (2020)
   PUBMED   33318477
  REMARK    GeneRIF: Downregulation of PA28alpha induces proteasome remodeling
            and results in resistance to proteasome inhibitors in multiple
            myeloma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 965)
  AUTHORS   Lesne,J., Locard-Paulet,M., Parra,J., Zivkovic,D., Menneteau,T.,
            Bousquet,M.P., Burlet-Schiltz,O. and Marcoux,J.
  TITLE     Conformational maps of human 20S proteasomes reveal PA28- and
            immuno-dependent inter-ring crosstalks
  JOURNAL   Nat Commun 11 (1), 6140 (2020)
   PUBMED   33262340
  REMARK    GeneRIF: Conformational maps of human 20S proteasomes reveal PA28-
            and immuno-dependent inter-ring crosstalks.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 965)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 965)
  AUTHORS   Ahn,K., Erlander,M., Leturcq,D., Peterson,P.A., Fruh,K. and Yang,Y.
  TITLE     In vivo characterization of the proteasome regulator PA28
  JOURNAL   J Biol Chem 271 (30), 18237-18242 (1996)
   PUBMED   8663520
REFERENCE   7  (bases 1 to 965)
  AUTHORS   Ahn,J.Y., Tanahashi,N., Akiyama,K., Hisamatsu,H., Noda,C.,
            Tanaka,K., Chung,C.H., Shibmara,N., Willy,P.J., Mott,J.D. et al.
  TITLE     Primary structures of two homologous subunits of PA28, a
            gamma-interferon-inducible protein activator of the 20S proteasome
  JOURNAL   FEBS Lett 366 (1), 37-42 (1995)
   PUBMED   7789512
REFERENCE   8  (bases 1 to 965)
  AUTHORS   Realini,C., Dubiel,W., Pratt,G., Ferrell,K. and Rechsteiner,M.
  TITLE     Molecular cloning and expression of a gamma-interferon-inducible
            activator of the multicatalytic protease
  JOURNAL   J Biol Chem 269 (32), 20727-20732 (1994)
   PUBMED   8051173
REFERENCE   9  (bases 1 to 965)
  AUTHORS   Honore,B., Leffers,H., Madsen,P. and Celis,J.E.
  TITLE     Interferon-gamma up-regulates a unique set of proteins in human
            keratinocytes. Molecular cloning and expression of the cDNA
            encoding the RGD-sequence-containing protein IGUP I-5111
  JOURNAL   Eur J Biochem 218 (2), 421-430 (1993)
   PUBMED   8269930
REFERENCE   10 (bases 1 to 965)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from L07633.1 and BM981616.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006263.3.
            
            Summary: The 26S proteasome is a multicatalytic proteinase complex
            with a highly ordered structure composed of 2 complexes, a 20S core
            and a 19S regulator. The 20S core is composed of 4 rings of 28
            non-identical subunits; 2 rings are composed of 7 alpha subunits
            and 2 rings are composed of 7 beta subunits. The 19S regulator is
            composed of a base, which contains 6 ATPase subunits and 2
            non-ATPase subunits, and a lid, which contains up to 10 non-ATPase
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. The immunoproteasome
            contains an alternate regulator, referred to as the 11S regulator
            or PA28, that replaces the 19S regulator. Three subunits (alpha,
            beta and gamma) of the 11S regulator have been identified. This
            gene encodes the alpha subunit of the 11S regulator, one of the two
            11S subunits that is induced by gamma-interferon. Three alpha and
            three beta subunits combine to form a heterohexameric ring.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.87432.1,
                                           SRR5189655.21876.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000206451.11/ ENSP00000206451.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-962               L07633.1           24-985
            963-965             BM981616.1         23-25               c
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q12"
     gene            1..965
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /note="proteasome activator subunit 1"
                     /db_xref="GeneID:5720"
                     /db_xref="HGNC:HGNC:9568"
                     /db_xref="MIM:600654"
     exon            1..108
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..819
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /note="isoform 1 is encoded by transcript variant 1; 29-kD
                     MCP activator subunit; interferon-gamma-inducible protein
                     5111; activator of multicatalytic protease subunit 1;
                     interferon-gamma IEF SSP 5111; interferon gamma
                     up-regulated I-5111 protein; epididymis secretory sperm
                     binding protein Li 129m; proteasome activator complex
                     subunit 1; IGUP I-5111; 11S regulator complex subunit
                     alpha; proteasome (prosome, macropain) activator subunit 1
                     (PA28 alpha)"
                     /codon_start=1
                     /product="proteasome activator complex subunit 1 isoform
                     1"
                     /protein_id="NP_006254.1"
                     /db_xref="CCDS:CCDS9612.1"
                     /db_xref="GeneID:5720"
                     /db_xref="HGNC:HGNC:9568"
                     /db_xref="MIM:600654"
                     /translation="MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFL
                     KEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCG
                     PVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELM
                     TSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVME
                     IRNAYAVLYDIILKNFEKLKKPRGETKGMIY"
     exon            109..141
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            142..204
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            205..315
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            316..361
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            362..459
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            460..528
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            529..596
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            597..651
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            652..738
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            739..965
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      942..947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /note="hexamer: AATAAA"
     polyA_site      965
                     /gene="PSME1"
                     /gene_synonym="HEL-S-129m; IFI5111; PA28A; PA28alpha;
                     REGalpha"
                     /note="major polyA site"
ORIGIN      
        1 accgctttcg ctttcccttc gcggtgccca ctccactcct tgtgcggcgc taggcccccc
       61 gtcccggtca tggccatgct cagggtccag cccgaggccc aagccaaggt ggatgtgttt
      121 cgtgaagacc tctgtaccaa gacagagaac ctgctcggga gctatttccc caagaagatt
      181 tctgagctgg atgcattttt aaaggagcca gctctcaatg aagccaactt gagcaatctg
      241 aaggccccat tggacatccc agtgcctgat ccagtcaagg agaaagagaa agaggagcgg
      301 aagaaacagc aggagaagga agacaaggat gaaaagaaga agggggagga tgaagacaaa
      361 ggtcctccct gtggcccagt gaactgcaat gaaaagatcg tggtccttct gcagcgcttg
      421 aagcctgaga tcaaggatgt cattgagcag ctcaacctgg tcaccacctg gttgcagctg
      481 cagatacctc ggattgagga tggtaacaat tttggagtgg ctgtccagga gaaggtgttt
      541 gagctgatga ccagcctcca caccaagcta gaaggcttcc acactcaaat ctctaagtat
      601 ttctctgagc gtggtgatgc agtgactaaa gcagccaagc agccccatgt gggtgattat
      661 cggcagctgg tgcacgagct ggatgaggca gagtaccggg acatccggct gatggtcatg
      721 gagatccgca atgcttatgc tgtgttatat gacatcatcc tgaagaactt cgagaagctc
      781 aagaagccca ggggagaaac aaagggaatg atctattgag agccctctct cccattctgt
      841 gatgagtaca gcagagacct tcctgctttt tactggggac tccagatttt ccccaaactt
      901 gcttctgttg agatttttcc ctcaccttgc ctctcaggca caataaatat agttatacca
      961 ctgcc
//
