LOCUS       NM_002812               1518 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens proteasome 26S subunit, non-ATPase 8 (PSMD8), mRNA.
ACCESSION   NM_002812
VERSION     NM_002812.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Li,X., Li,X., Hu,Y., Liu,O., Wang,Y., Li,S., Yang,Q. and Lin,B.
  TITLE     PSMD8 can serve as potential biomarker and therapeutic target of
            the PSMD family in ovarian cancer: based on bioinformatics analysis
            and in vitro validation
  JOURNAL   BMC Cancer 23 (1), 573 (2023)
   PUBMED   37349676
  REMARK    GeneRIF: PSMD8 can serve as potential biomarker and therapeutic
            target of the PSMD family in ovarian cancer: based on
            bioinformatics analysis and in vitro validation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1518)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1518)
  AUTHORS   Zhu,Y., Wang,W.L., Yu,D., Ouyang,Q., Lu,Y. and Mao,Y.
  TITLE     Structural mechanism for nucleotide-driven remodeling of the
            AAA-ATPase unfoldase in the activated human 26S proteasome
  JOURNAL   Nat Commun 9 (1), 1360 (2018)
   PUBMED   29636472
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1518)
  AUTHORS   Kottemann,M.C., Conti,B.A., Lach,F.P. and Smogorzewska,A.
  TITLE     Removal of RTF2 from Stalled Replisomes Promotes Maintenance of
            Genome Integrity
  JOURNAL   Mol Cell 69 (1), 24-35 (2018)
   PUBMED   29290612
REFERENCE   6  (bases 1 to 1518)
  AUTHORS   Simon,J.H., Gaddis,N.C., Fouchier,R.A. and Malim,M.H.
  TITLE     Evidence for a newly discovered cellular anti-HIV-1 phenotype
  JOURNAL   Nat Med 4 (12), 1397-1400 (1998)
   PUBMED   9846577
REFERENCE   7  (bases 1 to 1518)
  AUTHORS   Madani,N. and Kabat,D.
  TITLE     An endogenous inhibitor of human immunodeficiency virus in human
            lymphocytes is overcome by the viral Vif protein
  JOURNAL   J Virol 72 (12), 10251-10255 (1998)
   PUBMED   9811770
REFERENCE   8  (bases 1 to 1518)
  AUTHORS   Seeger,M., Ferrell,K., Frank,R. and Dubiel,W.
  TITLE     HIV-1 tat inhibits the 20 S proteasome and its 11 S
            regulator-mediated activation
  JOURNAL   J Biol Chem 272 (13), 8145-8148 (1997)
   PUBMED   9079628
REFERENCE   9  (bases 1 to 1518)
  AUTHORS   Coux,O., Tanaka,K. and Goldberg,A.L.
  TITLE     Structure and functions of the 20S and 26S proteasomes
  JOURNAL   Annu Rev Biochem 65, 801-847 (1996)
   PUBMED   8811196
  REMARK    Review article
REFERENCE   10 (bases 1 to 1518)
  AUTHORS   Kominami,K., DeMartino,G.N., Moomaw,C.R., Slaughter,C.A.,
            Shimbara,N., Fujimuro,M., Yokosawa,H., Hisamatsu,H., Tanahashi,N.,
            Shimizu,Y. et al.
  TITLE     Nin1p, a regulatory subunit of the 26S proteasome, is necessary for
            activation of Cdc28p kinase of Saccharomyces cerevisiae
  JOURNAL   EMBO J 14 (13), 3105-3115 (1995)
   PUBMED   7621825
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005789.1.
            
            On Jul 22, 2020 this sequence version replaced NM_002812.4.
            
            Summary: The 26S proteasome is a multicatalytic proteinase complex
            with a highly ordered structure composed of 2 complexes, a 20S core
            and a 19S regulator. The 20S core is composed of 4 rings of 28
            non-identical subunits; 2 rings are composed of 7 alpha subunits
            and 2 rings are composed of 7 beta subunits. The 19S regulator is
            composed of a base, which contains 6 ATPase subunits and 2
            non-ATPase subunits, and a lid, which contains up to 10 non-ATPase
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway.  An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            non-ATPase subunit of the 19S regulator. A pseudogene has been
            identified on chromosome 1. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU668354.1, BC001164.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000215071.9/ ENSP00000215071.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-391               AC005789.1         9062-9452
            392-464             AC005789.1         10651-10723
            465-567             AC005789.1         10843-10945
            568-733             AC005789.1         13731-13896
            734-834             AC005789.1         15390-15490
            835-946             AC005789.1         16608-16719
            947-1518            AC005789.1         17744-18315
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..1518
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /note="proteasome 26S subunit, non-ATPase 8"
                     /db_xref="GeneID:5714"
                     /db_xref="HGNC:HGNC:9566"
                     /db_xref="MIM:617844"
     exon            1..391
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..1084
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /note="26S proteasome regulatory subunit p31; epididymis
                     secretory sperm binding protein Li 91n; 26S proteasome
                     regulatory subunit S14; 26S proteasome regulatory subunit
                     RPN12; proteasome (prosome, macropain) 26S subunit,
                     non-ATPase, 8"
                     /codon_start=1
                     /product="26S proteasome non-ATPase regulatory subunit 8"
                     /protein_id="NP_002803.2"
                     /db_xref="CCDS:CCDS12515.2"
                     /db_xref="GeneID:5714"
                     /db_xref="HGNC:HGNC:9566"
                     /db_xref="MIM:617844"
                     /translation="MFIKGRAPRAPPRERRRATRGGLRQVVAPPRALGSTSRPHFRRA
                     SVCRRRCRKSGGLLAASRKMAAAAVNGAAGFSSSGPAATSGAVLQAATGMYEQLKGEW
                     NRKSPNLSKCGEELGRLKLVLLELNFLPTTGTKLTKQQLILARDILEIGAQWSILRKD
                     IPSFERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAK
                     DIQTNVYIKHPVSLEQYLMEGSYNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIE
                     KAYEKILFTEATRILFFNTPKKMTDYAKKRGWVLGPNNYYSFASQQQKPEDTTIPSTE
                     LAKQVIEYARQLEMIV"
     exon            392..464
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     exon            465..567
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     exon            568..733
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     exon            734..834
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     exon            835..946
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1518
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1152..1157
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /note="hexamer: AATAAA"
     polyA_site      1169
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /note="major polyA site"
     regulatory      1480..1485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /note="hexamer: AATAAA"
     regulatory      1498..1503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
                     /note="hexamer: ATTAAA"
     polyA_site      1503
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
     polyA_site      1518
                     /gene="PSMD8"
                     /gene_synonym="HEL-S-91n; HIP6; HYPF; Nin1p; p31; Rpn12;
                     S14"
ORIGIN      
        1 gagtgacgac agaggcggag ctccaactga catgttcatt aagggcaggg ctccgagggc
       61 gccacctcga gagcgacggc gggctacccg gggcgggctg aggcaggttg tagccccgcc
      121 ccgggccttg ggctccacct ctcggcccca cttccgccgg gcaagcgttt gtaggcggcg
      181 ctgccgtaaa tcaggcggtc tgcttgccgc atcacgcaag atggcggccg cggcggtgaa
      241 cggggcggca ggcttctcga gctccgggcc cgcggcaacc tcgggcgctg ttctgcaggc
      301 cgcgaccggc atgtacgagc aactcaaggg cgagtggaac cgtaaaagcc ccaatcttag
      361 caagtgcggg gaagagctgg gtcgactcaa gctagttctt ctggagctca acttcttgcc
      421 aaccacaggg accaagctga ccaaacagca gctaattctg gcccgtgaca tactggagat
      481 cggggcccaa tggagcatcc tacgcaagga catcccctcc ttcgagcgct acatggccca
      541 gctcaaatgc tactactttg attacaagga gcagctcccc gagtcagcct atatgcacca
      601 gctcttgggc ctcaacctcc tcttcctgct gtcccagaac cgggtggctg agttccacac
      661 ggagttggag cggctgcctg ccaaggacat acagaccaat gtctacatca agcacccagt
      721 gtccctggag caatacctga tggagggcag ctacaacaaa gtgttcctgg ccaagggtaa
      781 catccccgcc gagagctaca ccttcttcat tgacatcctg ctcgacacta tcagggatga
      841 gatcgctggg tgcatcgaga aggcctacga gaaaatcctt ttcactgagg ccacccggat
      901 cctcttcttc aacacaccca aaaagatgac agactacgcc aagaagcgag ggtgggtcct
      961 gggccccaac aactactaca gttttgccag ccagcagcag aagccggaag acaccaccat
     1021 tccctccaca gaactggcca aacaggtcat cgagtatgcc cggcagctgg agatgatcgt
     1081 ctgagccccc cgggcactgg gtggggcagg gcacgagtta tttaaaacag ttacactgca
     1141 gggtttcgcc caataaaggt ggactgacat tccctcttcc aggcccttgt ctccccagtt
     1201 gggacggcag agagacaagt tcttatatct gaagaacttg gaggttttgg ggcattcagg
     1261 agttggagat agcctccaac tgggtcagcc tctgtctggt gggcattgct cagggtctca
     1321 aacatggacg cccactgtgg ggccccagca gcactgtggc ctgcaggagg gcatggcccc
     1381 aggtaggggg actgttctag ccagctgtgg acacatagga atgctggacc agggtaccag
     1441 attttttcaa caaagggggt gaagtgtcct actaaaaaga ataaatgttg gcagtgaatt
     1501 aaacaatttt tcaaatga
//
