LOCUS       NM_002810               1319 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens proteasome 26S subunit ubiquitin receptor, non-ATPase
            4 (PSMD4), transcript variant 2, mRNA.
ACCESSION   NM_002810
VERSION     NM_002810.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Zhang,J., Fang,S., Rong,F., Jia,M., Wang,Y., Cui,H. and Hao,P.
  TITLE     PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2
            pathway and p53 inhibition
  JOURNAL   Hum Cell 36 (5), 1755-1772 (2023)
   PUBMED   37336868
  REMARK    GeneRIF: PSMD4 drives progression of hepatocellular carcinoma via
            Akt/COX2 pathway and p53 inhibition.
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1319)
  AUTHORS   Chevalier,B., Baatallah,N., Najm,M., Castanier,S., Jung,V.,
            Pranke,I., Golec,A., Stoven,V., Marullo,S., Antigny,F.,
            Guerrera,I.C., Sermet-Gaudelus,I., Edelman,A. and Hinzpeter,A.
  TITLE     Differential CFTR-Interactome Proximity Labeling Procedures
            Identify Enrichment in Multiple SLC Transporters
  JOURNAL   Int J Mol Sci 23 (16), 8937 (2022)
   PUBMED   36012204
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1319)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 1319)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   6  (bases 1 to 1319)
  AUTHORS   Ferrell,K., Deveraux,Q., van Nocker,S. and Rechsteiner,M.
  TITLE     Molecular cloning and expression of a multiubiquitin chain binding
            subunit of the human 26S protease
  JOURNAL   FEBS Lett 381 (1-2), 143-148 (1996)
   PUBMED   8641424
REFERENCE   7  (bases 1 to 1319)
  AUTHORS   Beal,R., Deveraux,Q., Xia,G., Rechsteiner,M. and Pickart,C.
  TITLE     Surface hydrophobic residues of multiubiquitin chains essential for
            proteolytic targeting
  JOURNAL   Proc Natl Acad Sci U S A 93 (2), 861-866 (1996)
   PUBMED   8570649
REFERENCE   8  (bases 1 to 1319)
  AUTHORS   Coux,O., Tanaka,K. and Goldberg,A.L.
  TITLE     Structure and functions of the 20S and 26S proteasomes
  JOURNAL   Annu Rev Biochem 65, 801-847 (1996)
   PUBMED   8811196
  REMARK    Review article
REFERENCE   9  (bases 1 to 1319)
  AUTHORS   Johansson,E., Lonnroth,I., Lange,S., Jonson,I., Jennische,E. and
            Lonnroth,C.
  TITLE     Molecular cloning and expression of a pituitary gland protein
            modulating intestinal fluid secretion
  JOURNAL   J Biol Chem 270 (35), 20615-20620 (1995)
   PUBMED   7657640
REFERENCE   10 (bases 1 to 1319)
  AUTHORS   Lonnroth,I. and Lange,S.
  TITLE     Purification and characterization of the antisecretory factor: a
            protein in the central nervous system and in the gut which inhibits
            intestinal hypersecretion induced by cholera toxin
  JOURNAL   Biochim Biophys Acta 883 (1), 138-144 (1986)
   PUBMED   3524692
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U24704.1 and AL391069.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 2, 2019 this sequence version replaced NM_002810.3.
            
            Summary: The 26S proteasome is a multicatalytic proteinase complex
            with a highly ordered structure composed of 2 complexes, a 20S core
            and a 19S regulator. The 20S core is composed of 4 rings of 28
            non-identical subunits; 2 rings are composed of 7 alpha subunits
            and 2 rings are composed of 7 beta subunits. The 19S regulator is
            composed of a base, which contains 6 ATPase subunits and 2
            non-ATPase subunits, and a lid, which contains up to 10 non-ATPase
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes one of
            the non-ATPase subunits of the 19S regulator lid. Pseudogenes have
            been identified on chromosomes 10 and 21. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U24704.1, BC072008.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368884.8/ ENSP00000357879.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1300              U24704.1           14-1313
            1301-1319           AL391069.11        4416-4434
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     gene            1..1319
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /note="proteasome 26S subunit ubiquitin receptor,
                     non-ATPase 4"
                     /db_xref="GeneID:5710"
                     /db_xref="HGNC:HGNC:9561"
                     /db_xref="MIM:601648"
     exon            1..75
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..1183
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /note="isoform 2 is encoded by transcript variant 2;
                     S5a/antisecretory factor protein; 26S proteasome
                     non-ATPase regulatory subunit 4; antisecretory factor 1;
                     angiocidin; multiubiquitin chain-binding protein; 26S
                     proteasome regulatory subunit S5A; proteasome (prosome,
                     macropain) 26S subunit, non-ATPase, 4; proteasome 26S
                     subunit, non-ATPase 4"
                     /codon_start=1
                     /product="26S proteasome non-ATPase regulatory subunit 4
                     isoform 2"
                     /protein_id="NP_002801.1"
                     /db_xref="CCDS:CCDS991.1"
                     /db_xref="GeneID:5710"
                     /db_xref="HGNC:HGNC:9561"
                     /db_xref="MIM:601648"
                     /translation="MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSN
                     PENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQG
                     KNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTL
                     NGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELAL
                     ALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLP
                     DLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEF
                     LQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK"
     exon            76..216
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            217..331
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            332..418
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            419..487
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            488..703
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            704..812
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            813..944
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1012
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1319
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1281..1286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /note="hexamer: AATAAA"
     polyA_site      1300
                     /gene="PSMD4"
                     /gene_synonym="AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A"
                     /note="major polyA site"
ORIGIN      
        1 gttgttaggc cgtcccggag acccggtcgg gagggaggaa ggtggcaaga tggtgttgga
       61 aagcactatg gtgtgtgtgg acaacagtga gtatatgcgg aatggagact tcttacccac
      121 caggctgcag gcccagcagg atgctgtcaa catagtttgt cattcaaaga cccgcagcaa
      181 ccctgagaac aacgtgggcc ttatcacact ggctaatgac tgtgaagtgc tgaccacact
      241 caccccagac actggccgta tcctgtccaa gctacatact gtccaaccca agggcaagat
      301 caccttctgc acgggcatcc gcgtggccca tctggctctg aagcaccgac aaggcaagaa
      361 tcacaagatg cgcatcattg cctttgtggg aagcccagtg gaggacaatg agaaggatct
      421 ggtgaaactg gctaaacgcc tcaagaagga gaaagtaaat gttgacatta tcaattttgg
      481 ggaagaggag gtgaacacag aaaagctgac agcctttgta aacacgttga atggcaaaga
      541 tggaaccggt tctcatctgg tgacagtgcc tcctgggccc agtttggctg atgctctcat
      601 cagttctccg attttggctg gtgaaggtgg tgccatgctg ggtcttggtg ccagtgactt
      661 tgaatttgga gtagatccca gtgctgatcc tgagctggcc ttggcccttc gtgtatctat
      721 ggaagagcag cggcagcggc aggaggagga ggcccggcgg gcagctgcag cttctgctgc
      781 tgaggccggg attgctacga ctgggactga agactcagac gatgccctgc tgaagatgac
      841 catcagccag caagagtttg gccgcactgg gcttcctgac ctaagcagta tgactgagga
      901 agagcagatt gcttatgcca tgcagatgtc cctgcaggga gcagagtttg gccaggcgga
      961 atcagcagac attgatgcca gctcagctat ggacacatct gagccagcca aggaggagga
     1021 tgattacgac gtgatgcagg accccgagtt ccttcagagt gtcctagaga acctcccagg
     1081 tgtggatccc aacaatgaag ccattcgaaa tgctatgggc tccctggcct cccaggccac
     1141 caaggacggc aagaaggaca agaaggagga agacaagaag tgagactgga gggaaagggt
     1201 agctgagtct gcttagggga ctgcatggga agcacggaat atagggttag atgtgtgtta
     1261 tctgtaacca ttacagccta aataaagctt ggcaactttt tttccttttt tgcttcaaa
//
