LOCUS       NM_148919               1124 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit beta 8 (PSMB8), transcript
            variant 2, mRNA.
ACCESSION   NM_148919
VERSION     NM_148919.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Terlikowska-Brzosko,A., Galus,R., Murawski,P.,
            Niderla-Bielinska,J., Mlynarczuk-Bialy,I., Paluchowska,E. and
            Owczarek,W.
  TITLE     Human Beta Defensin-2 mRNA and Proteasome Subunit beta Type 8 mRNA
            Analysis, Useful in Differentiating Skin Biopsies from Atopic
            Dermatitis and Psoriasis Vulgaris Patients
  JOURNAL   Int J Mol Sci 25 (17), 9192 (2024)
   PUBMED   39273140
  REMARK    GeneRIF: Human Beta Defensin-2 mRNA and Proteasome Subunit beta
            Type 8 mRNA Analysis, Useful in Differentiating Skin Biopsies from
            Atopic Dermatitis and Psoriasis Vulgaris Patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Ou,W.T., Wan,Q.X., Wu,Y.B., Sun,X., Li,Y.L., Tang,D., Zhang,J.,
            Li,S.S., Wang,N.Y., Liu,Z.L. and Wu,J.J.
  TITLE     Long Noncoding RNA PSMB8-AS1 Mediates the
            Tobacco-Carcinogen-Induced Transformation of a Human Bronchial
            Epithelial Cell Line by Regulating Cell Cycle
  JOURNAL   Chem Res Toxicol 37 (6), 957-967 (2024)
   PUBMED   38771128
  REMARK    GeneRIF: Long Noncoding RNA PSMB8-AS1 Mediates the
            Tobacco-Carcinogen-Induced Transformation of a Human Bronchial
            Epithelial Cell Line by Regulating Cell Cycle.
REFERENCE   3  (bases 1 to 1124)
  AUTHORS   Hou,L. and Zhang,Y.
  TITLE     Peripheral blood mononuclear cell low molecular mass protein 7 in
            acute ischemic stroke: vertical change from admission to discharge
            and correlation with disability, stroke recurrence, and death
  JOURNAL   Front Immunol 15, 1296835 (2024)
   PUBMED   38404572
  REMARK    GeneRIF: Peripheral blood mononuclear cell low molecular mass
            protein 7 in acute ischemic stroke: vertical change from admission
            to discharge and correlation with disability, stroke recurrence,
            and death.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1124)
  AUTHORS   Zhong,Y., Yu,F., Yang,L., Wang,Y., Liu,L., Jia,C., Cai,H., Yang,J.,
            Sheng,S., Lv,Z., Weng,L., Wu,B. and Zhang,X.
  TITLE     HOXD9/miR-451a/PSMB8 axis is implicated in the regulation of cell
            proliferation and metastasis via PI3K/AKT signaling pathway in
            human anaplastic thyroid carcinoma
  JOURNAL   J Transl Med 21 (1), 817 (2023)
   PUBMED   37974228
  REMARK    GeneRIF: HOXD9/miR-451a/PSMB8 axis is implicated in the regulation
            of cell proliferation and metastasis via PI3K/AKT signaling pathway
            in human anaplastic thyroid carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1124)
  AUTHORS   Ustrell,V., Realini,C., Pratt,G. and Rechsteiner,M.
  TITLE     Human lymphoblast and erythrocyte multicatalytic proteases:
            differential peptidase activities and responses to the 11S
            regulator
  JOURNAL   FEBS Lett 376 (3), 155-158 (1995)
   PUBMED   7498531
REFERENCE   6  (bases 1 to 1124)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   7  (bases 1 to 1124)
  AUTHORS   Meinhardt,T., Graf,U. and Hammerling,G.J.
  TITLE     Different genomic structure of mouse and human Lmp7 genes:
            characterization of MHC-encoded proteasome genes
  JOURNAL   Immunogenetics 38 (5), 373-379 (1993)
   PUBMED   8344725
REFERENCE   8  (bases 1 to 1124)
  AUTHORS   Beck,S., Kelly,A., Radley,E., Khurshid,F., Alderton,R.P. and
            Trowsdale,J.
  TITLE     DNA sequence analysis of 66 kb of the human MHC class II region
            encoding a cluster of genes for antigen processing
  JOURNAL   J Mol Biol 228 (2), 433-441 (1992)
   PUBMED   1453454
REFERENCE   9  (bases 1 to 1124)
  AUTHORS   Fruh,K., Yang,Y., Arnold,D., Chambers,J., Wu,L., Waters,J.B.,
            Spies,T. and Peterson,P.A.
  TITLE     Alternative exon usage and processing of the major
            histocompatibility complex-encoded proteasome subunits
  JOURNAL   J Biol Chem 267 (31), 22131-22140 (1992)
   PUBMED   1429565
REFERENCE   10 (bases 1 to 1124)
  AUTHORS   Glynne,R., Powis,S.H., Beck,S., Kelly,A., Kerr,L.A. and
            Trowsdale,J.
  TITLE     A proteasome-related gene between the two ABC transporter loci in
            the class II region of the human MHC
  JOURNAL   Nature 353 (6342), 357-360 (1991)
   PUBMED   1922342
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG485076.1, BM781620.1 and
            BC001114.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 6, 2018 this sequence version replaced NM_148919.3.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the proteasome B-type family, also known as the T1B
            family, that is a 20S core beta subunit. This gene is located in
            the class II region of the MHC (major histocompatibility complex).
            Expression of this gene is induced by gamma interferon and this
            gene product replaces catalytic subunit 3 (proteasome beta 5
            subunit) in the immunoproteasome. Proteolytic processing is
            required to generate a mature subunit. Two alternative transcripts
            encoding two isoforms have been identified; both isoforms are
            processed to yield the same mature subunit. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            contains an alternate in-frame exon in the 5' coding region,
            compared to variant 1. Isoform E2 has a distinct N-terminus,
            compared to isoform E1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.102895.1,
                                           SRR1163658.459556.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2153946
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374882.8/ ENSP00000364016.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BG485076.1         2-9
            9-673               BM781620.1         1-665
            674-1124            BC001114.2         849-1299
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.32"
     gene            1..1124
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /note="proteasome 20S subunit beta 8"
                     /db_xref="GeneID:5696"
                     /db_xref="HGNC:HGNC:9545"
                     /db_xref="MIM:177046"
     exon            1..198
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..882
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /EC_number="3.4.25.1"
                     /note="isoform E2 precursor is encoded by transcript
                     variant 2; proteasome catalytic subunit 3i; proteasome
                     subunit beta 5i; proteasome subunit Y2; low molecular
                     weight protein 7; proteasome-related gene 7; proteasome
                     (prosome, macropain) subunit, beta type, 8 (large
                     multifunctional peptidase 7); multicatalytic endopeptidase
                     complex subunit C13; macropain subunit C13; protease
                     component C13; proteasome subunit beta 8; proteasome
                     component C13; really interesting new gene 10 protein; low
                     molecular mass protein 7"
                     /codon_start=1
                     /product="proteasome subunit beta type-8 isoform E2
                     precursor"
                     /protein_id="NP_683720.2"
                     /db_xref="CCDS:CCDS4757.1"
                     /db_xref="GeneID:5696"
                     /db_xref="HGNC:HGNC:9545"
                     /db_xref="MIM:177046"
                     /translation="MALLDVCGAPRGQRPESALPVAGSGRRSDPGHYSFSMRSPELAL
                     PRGMQPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALR
                     VNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMC
                     QYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPN
                     LSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ
                     "
     exon            199..346
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /inference="alignment:Splign:2.1.0"
     exon            347..458
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /inference="alignment:Splign:2.1.0"
     exon            459..588
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /inference="alignment:Splign:2.1.0"
     exon            589..793
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /inference="alignment:Splign:2.1.0"
     exon            794..1124
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1105..1110
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /note="hexamer: AAGAAA"
     polyA_site      1124
                     /gene="PSMB8"
                     /gene_synonym="ALDD; D6S216; D6S216E; JMP; LMP7; NKJO;
                     PRAAS1; PSMB5i; RING10"
                     /note="major polyA site"
ORIGIN      
        1 gctttcactt cctcctccga gagcggacag atctctgggt gctgggcggt catggcgcta
       61 ctagatgtat gcggagcccc ccgagggcag cggccggaat cggctctccc ggttgcggga
      121 agcgggcgtc gctcggaccc aggacactac agtttctcta tgcgatctcc agagctcgct
      181 ttaccccggg gaatgcagcc cacagaattc ttccagtccc tgggtgggga cggagaaagg
      241 aacgttcaga ttgagatggc ccatggcacc accacgctcg ccttcaagtt ccagcatgga
      301 gtgattgcag cagtggattc tcgggcctca gctgggtcct acattagtgc cttacgggtg
      361 aacaaggtga ttgagattaa cccttacctg cttggcacca tgtctggctg tgcagcagac
      421 tgtcagtact gggagcgcct gctggccaag gaatgcaggc tgtactatct gcgaaatgga
      481 gaacgtattt cagtgtcggc agcctccaag ctgctgtcca acatgatgtg ccagtaccgg
      541 ggcatgggcc tctctatggg cagtatgatc tgtggctggg ataagaaggg tcctggactc
      601 tactacgtgg atgaacatgg gactcggctc tcaggaaata tgttctccac gggtagtggg
      661 aacacttatg cctacggggt catggacagt ggctatcggc ctaatcttag ccctgaagag
      721 gcctatgacc ttggccgcag ggctattgct tatgccactc acagagacag ctattctgga
      781 ggcgttgtca atatgtacca catgaaggaa gatggttggg tgaaagtaga aagtacagat
      841 gtcagtgacc tgctgcacca gtaccgggaa gccaatcaat aatggtggtg gtggcagctg
      901 ggcaggtctc ctctgggagg tcttggccga ctcagggacc taagccacgt taagtccaag
      961 gagaagaaga ggcctagcct gagccaaaga gagagtacgg gctcagcagc cagaggaggc
     1021 cggtgaagtg catcttctgc gtgttctcta tttgaacaag catttccccc agggaagttt
     1081 ctgggtgccc cactaagtag aataaagaaa aacggttata aata
//
