LOCUS       NM_002797               1044 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit beta 5 (PSMB5), transcript
            variant 1, mRNA.
ACCESSION   NM_002797
VERSION     NM_002797.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Wang,H., Bai,C., Dang,X. and Wang,H.
  TITLE     MiR-383 sensitizes osteosarcoma cells to bortezomib treatment via
            down-regulating PSMB5
  JOURNAL   Mol Biol Rep 51 (1), 170 (2024)
   PUBMED   38252234
  REMARK    GeneRIF: MiR-383 sensitizes osteosarcoma cells to bortezomib
            treatment via down-regulating PSMB5.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Liu,J., Mi,J., Liu,S., Chen,H. and Jiang,L.
  TITLE     PSMB5 overexpression is correlated with tumor proliferation and
            poor prognosis in hepatocellular carcinoma
  JOURNAL   FEBS Open Bio 12 (11), 2025-2041 (2022)
   PUBMED   36062301
  REMARK    GeneRIF: PSMB5 overexpression is correlated with tumor
            proliferation and poor prognosis in hepatocellular carcinoma.
REFERENCE   3  (bases 1 to 1044)
  AUTHORS   Zhao,J., Makhija,S., Zhou,C., Zhang,H., Wang,Y., Muralidharan,M.,
            Huang,B. and Cheng,Y.
  TITLE     Structural insights into the human PA28-20S proteasome enabled by
            efficient tagging and purification of endogenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 119 (33), e2207200119 (2022)
   PUBMED   35858375
REFERENCE   4  (bases 1 to 1044)
  AUTHORS   Kong,H.E., Lim,J., Linsalata,A., Kang,Y., Malik,I., Allen,E.G.,
            Cao,Y., Shubeck,L., Johnston,R., Huang,Y., Gu,Y., Guo,X.,
            Zwick,M.E., Qin,Z., Wingo,T.S., Juncos,J., Nelson,D.L.,
            Epstein,M.P., Cutler,D.J., Todd,P.K., Sherman,S.L., Warren,S.T. and
            Jin,P.
  TITLE     Identification of PSMB5 as a genetic modifier of fragile
            X-associated tremor/ataxia syndrome
  JOURNAL   Proc Natl Acad Sci U S A 119 (22), e2118124119 (2022)
   PUBMED   35617426
  REMARK    GeneRIF: Identification of PSMB5 as a genetic modifier of fragile
            X-associated tremor/ataxia syndrome.
REFERENCE   5  (bases 1 to 1044)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 1044)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 207 (3), 1059 (1995)
   PUBMED   7864893
  REMARK    Correction to:[Biochem Biophys Res Commun. 1994 Dec
            30;205(3):1785-9. doi: 10.1006/bbrc.1994.2876.. PMID: 7811265]
REFERENCE   7  (bases 1 to 1044)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   8  (bases 1 to 1044)
  AUTHORS   Belich,M.P., Glynne,R.J., Senger,G., Sheer,D. and Trowsdale,J.
  TITLE     Proteasome components with reciprocal expression to that of the
            MHC-encoded LMP proteins
  JOURNAL   Curr Biol 4 (9), 769-776 (1994)
   PUBMED   7820546
REFERENCE   9  (bases 1 to 1044)
  AUTHORS   Akiyama,K., Yokota,K., Kagawa,S., Shimbara,N., Tamura,T.,
            Akioka,H., Nothwang,H.G., Noda,C., Tanaka,K. and Ichihara,A.
  TITLE     cDNA cloning and interferon gamma down-regulation of proteasomal
            subunits X and Y
  JOURNAL   Science 265 (5176), 1231-1234 (1994)
   PUBMED   8066462
REFERENCE   10 (bases 1 to 1044)
  AUTHORS   Lee,L.W., Moomaw,C.R., Orth,K., McGuire,M.J., DeMartino,G.N. and
            Slaughter,C.A.
  TITLE     Relationships among the subunits of the high molecular weight
            proteinase, macropain (proteasome)
  JOURNAL   Biochim Biophys Acta 1037 (2), 178-185 (1990)
   PUBMED   2306472
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC057840.1 and BC107720.1.
            
            On May 17, 2019 this sequence version replaced NM_002797.4.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the proteasome B-type family, also known as the T1B
            family, that is a 20S core beta subunit in the proteasome. This
            catalytic subunit is not present in the immunoproteasome and is
            replaced by catalytic subunit 3i (proteasome beta 8 subunit).
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Jan 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longest protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189661.30993.1,
                                           SRR5189661.45886.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142680, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361611.11/ ENSP00000355325.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-321               BC057840.1         1-321
            322-1044            BC107720.1         318-1040
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..1044
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /note="proteasome 20S subunit beta 5"
                     /db_xref="GeneID:5693"
                     /db_xref="HGNC:HGNC:9542"
                     /db_xref="MIM:600306"
     exon            1..217
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..811
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /EC_number="3.4.25.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proteasome subunit MB1; beta-5; PSX large multifunctional
                     protease X; proteasome epsilon chain; macropain epsilon
                     chain; multicatalytic endopeptidase complex epsilon chain;
                     proteasome chain 6; proteasome catalytic subunit 3;
                     proteasome subunit, beta type, 5; proteasome subunit X;
                     proteasome beta 5 subunit; proteasome (prosome, macropain)
                     subunit, beta type, 5; testicular tissue protein Li 153;
                     proteasome subunit beta 5"
                     /codon_start=1
                     /product="proteasome subunit beta type-5 isoform 1"
                     /protein_id="NP_002788.1"
                     /db_xref="CCDS:CCDS9584.1"
                     /db_xref="GeneID:5693"
                     /db_xref="HGNC:HGNC:9542"
                     /db_xref="MIM:600306"
                     /translation="MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAP
                     GWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLG
                     TMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMI
                     CGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRA
                     IYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP"
     exon            218..524
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /inference="alignment:Splign:2.1.0"
     exon            525..1044
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1024..1029
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /note="hexamer: ATTACA"
     polyA_site      1044
                     /gene="PSMB5"
                     /gene_synonym="LMPX; MB1"
                     /note="major polyA site"
ORIGIN      
        1 ctttctgccc acactagaca tggcgcttgc cagcgtgttg gagagaccgc taccggtgaa
       61 ccagcgcggg tttttcggac ttgggggtcg tgcagatctg ctggatctag gtccagggag
      121 tctcagtgat ggtctgagcc tggccgcgcc aggctggggt gtcccagaag agccaggaat
      181 cgaaatgctt catggaacaa ccaccctggc cttcaagttc cgccatggag tcatagttgc
      241 agctgactcc agggctacag cgggtgctta cattgcctcc cagacggtga agaaggtgat
      301 agagatcaac ccatacctgc taggcaccat ggctgggggc gcagcggatt gcagcttctg
      361 ggaacggctg ttggctcggc aatgtcgaat ctatgagctt cgaaataagg aacgcatctc
      421 tgtagcagct gcctccaaac tgcttgccaa catggtgtat cagtacaaag gcatggggct
      481 gtccatgggc accatgatct gtggctggga taagagaggc cctggcctct actacgtgga
      541 cagtgaaggg aaccggattt caggggccac cttctctgta ggttctggct ctgtgtatgc
      601 atatggggtc atggatcggg gctattccta tgacctggaa gtggagcagg cctatgatct
      661 ggcccgtcga gccatctacc aagccaccta cagagatgcc tactcaggag gtgcagtcaa
      721 cctctaccac gtgcgggagg atggctggat ccgagtctcc agtgacaatg tggctgatct
      781 acatgagaag tatagtggct ctaccccctg aaagagggtg gatgcagctg cttgtgtttc
      841 ttggggtgac tgtcattggt aatacggaca cagtgaccca tcctccatcc tatttatagt
      901 ggaagggcct tcaattgtat cagtactttt ttttaagctc tggcacattg acctctatgt
      961 gttaccagtc attaatgagc tgctgcagag gtgactattt gttttacttt cttggatgtt
     1021 aacattacac tactcactac tcaa
//
