LOCUS       NM_002796                918 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit beta 4 (PSMB4), mRNA.
ACCESSION   NM_002796
VERSION     NM_002796.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 918)
  AUTHORS   de Almeida,M., Hinterndorfer,M., Brunner,H., Grishkovskaya,I.,
            Singh,K., Schleiffer,A., Jude,J., Deswal,S., Kalis,R., Vunjak,M.,
            Lendl,T., Imre,R., Roitinger,E., Neumann,T., Kandolf,S.,
            Schutzbier,M., Mechtler,K., Versteeg,G.A., Haselbach,D. and
            Zuber,J.
  TITLE     AKIRIN2 controls the nuclear import of proteasomes in vertebrates
  JOURNAL   Nature 599 (7885), 491-496 (2021)
   PUBMED   34711951
REFERENCE   3  (bases 1 to 918)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 918)
  AUTHORS   Montenegro-Venegas,C., Fienko,S., Anni,D., Pina-Fernandez,E.,
            Frischknecht,R. and Fejtova,A.
  TITLE     Bassoon inhibits proteasome activity via interaction with PSMB4
  JOURNAL   Cell Mol Life Sci 78 (4), 1545-1563 (2021)
   PUBMED   32651614
  REMARK    GeneRIF: Bassoon inhibits proteasome activity via interaction with
            PSMB4.
REFERENCE   5  (bases 1 to 918)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 918)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   7  (bases 1 to 918)
  AUTHORS   Nothwang,H.G., Tamura,T., Tanaka,K. and Ichihara,A.
  TITLE     Sequence analyses and inter-species comparisons of three novel
            human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine
            potential proteolytic active-site residues
  JOURNAL   Biochim Biophys Acta 1219 (2), 361-368 (1994)
   PUBMED   7918633
REFERENCE   8  (bases 1 to 918)
  AUTHORS   Gerards,W.L., Hop,F.W., Hendriks,I.L. and Bloemendal,H.
  TITLE     Cloning and expression of a human pro(tea)some beta-subunit cDNA: a
            homologue of the yeast PRE4-subunit essential for
            peptidylglutamyl-peptide hydrolase activity
  JOURNAL   FEBS Lett 346 (2-3), 151-155 (1994)
   PUBMED   8013624
REFERENCE   9  (bases 1 to 918)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   10 (bases 1 to 918)
  AUTHORS   Lee,L.W., Moomaw,C.R., Orth,K., McGuire,M.J., DeMartino,G.N. and
            Slaughter,C.A.
  TITLE     Relationships among the subunits of the high molecular weight
            proteinase, macropain (proteasome)
  JOURNAL   Biochim Biophys Acta 1037 (2), 178-185 (1990)
   PUBMED   2306472
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL589764.19.
            
            On Nov 25, 2018 this sequence version replaced NM_002796.2.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the proteasome B-type family, also known as the T1B
            family, that is a 20S core beta subunit. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX394340.2, BM479861.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000290541.7/ ENSP00000290541.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-155               AL589764.19        17171-17325
            156-362             AL589764.19        17579-17785
            363-509             AL589764.19        18040-18186
            510-591             AL589764.19        18362-18443
            592-708             AL589764.19        18837-18953
            709-797             AL589764.19        19140-19228
            798-918             AL589764.19        19415-19535
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     gene            1..918
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /note="proteasome 20S subunit beta 4"
                     /db_xref="GeneID:5692"
                     /db_xref="HGNC:HGNC:9541"
                     /db_xref="MIM:602177"
     exon            1..155
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..810
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /EC_number="3.4.25.1"
                     /note="proteasome subunit HsN3; proteasome beta chain;
                     macropain beta chain; proteasome chain 3; multicatalytic
                     endopeptidase complex beta chain; proteasome subunit, beta
                     type, 4; hsBPROS26; 26 kDa prosomal protein; testis tissue
                     sperm-binding protein Li 79P; proteasome (prosome,
                     macropain) subunit, beta type, 4; proteasome subunit beta
                     4; proteasome subunit beta7; beta-7; proteasome subunit
                     beta-7"
                     /codon_start=1
                     /product="proteasome subunit beta type-4"
                     /protein_id="NP_002787.2"
                     /db_xref="CCDS:CCDS996.1"
                     /db_xref="GeneID:5692"
                     /db_xref="HGNC:HGNC:9541"
                     /db_xref="MIM:602177"
                     /translation="MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPIT
                     RTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDY
                     ADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGY
                     ADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCM
                     RVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIAHMISGFE"
     exon            156..362
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     exon            363..509
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     exon            510..591
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     exon            592..708
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     exon            709..797
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     exon            798..918
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /inference="alignment:Splign:2.1.0"
     regulatory      900..905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /note="hexamer: AATAAA"
     polyA_site      918
                     /gene="PSMB4"
                     /gene_synonym="HN3; HsN3; PRAAS3; PROS-26; PROS26"
                     /note="major polyA site"
ORIGIN      
        1 gctaccgtga ctaagatgga agcgtttttg gggtcgcggt ccggactttg ggcggggggt
       61 ccggccccag gacagtttta ccgcattccg tccactcccg attccttcat ggatccggcg
      121 tctgcacttt acagaggtcc aatcacgcgg acccagaacc ccatggtgac cgggacctca
      181 gtcctcggcg ttaagttcga gggcggagtg gtgattgccg cagacatgct gggatcctac
      241 ggctccttgg ctcgtttccg caacatctct cgcattatgc gagtcaacaa cagtaccatg
      301 ctgggtgcct ctggcgacta cgctgatttc cagtatttga agcaagttct cggccagatg
      361 gtgattgatg aggagcttct gggagatgga cacagctata gtcctagagc tattcattca
      421 tggctgacca gggccatgta cagccggcgc tcgaagatga accctttgtg gaacaccatg
      481 gtcatcggag gctatgctga tggagagagc ttcctcggtt atgtggacat gcttggtgta
      541 gcctatgaag ccccttcgct ggccactggt tatggtgcat acttggctca gcctctgctg
      601 cgagaagttc tggagaagca gccagtgcta agccagaccg aggcccgcga cttagtagaa
      661 cgctgcatgc gagtgctgta ctaccgagat gcccgttctt acaaccggtt tcaaatcgcc
      721 actgtcaccg aaaaaggtgt tgaaatagag ggaccattgt ctacagagac caactgggat
      781 attgcccaca tgatcagtgg ctttgaatga aatacagatg cattatccag aactgaagtt
      841 gccctacttt taactttgaa cttggctagt tcaaagatag actcttcttt tgtaaagtaa
      901 ataaattctt caaaatgc
//
