LOCUS       NM_002795                762 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit beta 3 (PSMB3), transcript
            variant 1, mRNA.
ACCESSION   NM_002795
VERSION     NM_002795.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Zhao,J., Makhija,S., Zhou,C., Zhang,H., Wang,Y., Muralidharan,M.,
            Huang,B. and Cheng,Y.
  TITLE     Structural insights into the human PA28-20S proteasome enabled by
            efficient tagging and purification of endogenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 119 (33), e2207200119 (2022)
   PUBMED   35858375
REFERENCE   2  (bases 1 to 762)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 762)
  AUTHORS   de Almeida,M., Hinterndorfer,M., Brunner,H., Grishkovskaya,I.,
            Singh,K., Schleiffer,A., Jude,J., Deswal,S., Kalis,R., Vunjak,M.,
            Lendl,T., Imre,R., Roitinger,E., Neumann,T., Kandolf,S.,
            Schutzbier,M., Mechtler,K., Versteeg,G.A., Haselbach,D. and
            Zuber,J.
  TITLE     AKIRIN2 controls the nuclear import of proteasomes in vertebrates
  JOURNAL   Nature 599 (7885), 491-496 (2021)
   PUBMED   34711951
REFERENCE   4  (bases 1 to 762)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 762)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 762)
  AUTHORS   McCusker,D., Jones,T., Sheer,D. and Trowsdale,J.
  TITLE     Genetic relationships of the genes encoding the human proteasome
            beta subunits and the proteasome PA28 complex
  JOURNAL   Genomics 45 (2), 362-367 (1997)
   PUBMED   9344661
REFERENCE   7  (bases 1 to 762)
  AUTHORS   Seeger,M., Ferrell,K., Frank,R. and Dubiel,W.
  TITLE     HIV-1 tat inhibits the 20 S proteasome and its 11 S
            regulator-mediated activation
  JOURNAL   J Biol Chem 272 (13), 8145-8148 (1997)
   PUBMED   9079628
REFERENCE   8  (bases 1 to 762)
  AUTHORS   Coux,O., Tanaka,K. and Goldberg,A.L.
  TITLE     Structure and functions of the 20S and 26S proteasomes
  JOURNAL   Annu Rev Biochem 65, 801-847 (1996)
   PUBMED   8811196
  REMARK    Review article
REFERENCE   9  (bases 1 to 762)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   10 (bases 1 to 762)
  AUTHORS   Nothwang,H.G., Tamura,T., Tanaka,K. and Ichihara,A.
  TITLE     Sequence analyses and inter-species comparisons of three novel
            human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine
            potential proteolytic active-site residues
  JOURNAL   Biochim Biophys Acta 1219 (2), 361-368 (1994)
   PUBMED   7918633
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG942618.1, BC002570.1 and
            BC013008.2.
            
            On Nov 23, 2018 this sequence version replaced NM_002795.3.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the proteasome B-type family, also known as the T1B
            family, that is a 20S core beta subunit. The 26 S proteasome may be
            involved in trinucleotide repeat expansion, a phenomenon which is
            associated with many hereditary neurological diseases. Pseudogenes
            have been identified on chromosomes 2 and 12. Alternative splicing
            results in multiple transcript variants [provided by RefSeq, Sep
            2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the functional protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.151399.1,
                                           SRR5189658.160197.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000619426.5/ ENSP00000483688.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                BG942618.1         30-96
            68-68               BC002570.1         35-35
            69-762              BC013008.2         60-753
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..762
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /note="proteasome 20S subunit beta 3"
                     /db_xref="GeneID:5691"
                     /db_xref="HGNC:HGNC:9540"
                     /db_xref="MIM:602176"
     exon            1..89
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..704
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /EC_number="3.4.25.1"
                     /note="proteasome theta chain; proteasome (prosome,
                     macropain) subunit, beta type, 3; proteasome chain 13;
                     proteasome component C10-II; epididymis secretory sperm
                     binding protein; proteasome subunit beta 3; beta-3"
                     /codon_start=1
                     /product="proteasome subunit beta type-3"
                     /protein_id="NP_002786.2"
                     /db_xref="CCDS:CCDS11328.1"
                     /db_xref="GeneID:5691"
                     /db_xref="HGNC:HGNC:9540"
                     /db_xref="MIM:602176"
                     /translation="MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFP
                     MGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGP
                     YYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDP
                     DHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD"
     exon            90..274
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /inference="alignment:Splign:2.1.0"
     exon            275..382
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /inference="alignment:Splign:2.1.0"
     exon            383..560
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /inference="alignment:Splign:2.1.0"
     exon            561..655
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /inference="alignment:Splign:2.1.0"
     exon            656..762
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      742..747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /note="hexamer: AATAAA"
     polyA_site      762
                     /gene="PSMB3"
                     /gene_synonym="HC10-II"
                     /note="major polyA site"
ORIGIN      
        1 agcagtgaga gcggttgcgc agtgaaggct agacccggtt tactggaatt gctctggcga
       61 tcgagggatc ctagtacacc gcaatcatgt ctattatgtc ctataacgga ggggccgtca
      121 tggccatgaa ggggaagaac tgtgtggcca tcgctgcaga caggcgcttc gggatccagg
      181 cccagatggt gaccacggac ttccagaaga tctttcccat gggtgaccgg ctgtacatcg
      241 gtctggccgg gctcgccact gacgtccaga cagttgccca gcgcctcaag ttccggctga
      301 acctgtatga gttgaaggaa ggtcggcaga tcaaacctta taccctcatg agcatggtgg
      361 ccaacctctt gtatgagaaa cggtttggcc cttactacac tgagccagtc attgccgggt
      421 tggacccgaa gacctttaag cccttcattt gctctctaga cctcatcggc tgccccatgg
      481 tgactgatga ctttgtggtc agtggcacct gcgccgaaca aatgtacgga atgtgtgagt
      541 ccctctggga gcccaacatg gatccggatc acctgtttga aaccatctcc caagccatgc
      601 tgaatgctgt ggaccgggat gcagtgtcag gcatgggagt cattgtccac atcatcgaga
      661 aggacaaaat caccaccagg acactgaagg cccgaatgga ctaaccctgt tcccagagcc
      721 cacttttttt tctttttttg aaataaaata gcctgtcttt ca
//
