LOCUS       NM_002794               4426 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript
            variant 1, mRNA.
ACCESSION   NM_002794
VERSION     NM_002794.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Zhao,J., Makhija,S., Zhou,C., Zhang,H., Wang,Y., Muralidharan,M.,
            Huang,B. and Cheng,Y.
  TITLE     Structural insights into the human PA28-20S proteasome enabled by
            efficient tagging and purification of endogenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 119 (33), e2207200119 (2022)
   PUBMED   35858375
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 4426)
  AUTHORS   de Almeida,M., Hinterndorfer,M., Brunner,H., Grishkovskaya,I.,
            Singh,K., Schleiffer,A., Jude,J., Deswal,S., Kalis,R., Vunjak,M.,
            Lendl,T., Imre,R., Roitinger,E., Neumann,T., Kandolf,S.,
            Schutzbier,M., Mechtler,K., Versteeg,G.A., Haselbach,D. and
            Zuber,J.
  TITLE     AKIRIN2 controls the nuclear import of proteasomes in vertebrates
  JOURNAL   Nature 599 (7885), 491-496 (2021)
   PUBMED   34711951
REFERENCE   4  (bases 1 to 4426)
  AUTHORS   Sahu,I., Mali,S.M., Sulkshane,P., Xu,C., Rozenberg,A., Morag,R.,
            Sahoo,M.P., Singh,S.K., Ding,Z., Wang,Y., Day,S., Cong,Y.,
            Kleifeld,O., Brik,A. and Glickman,M.H.
  TITLE     The 20S as a stand-alone proteasome in cells can degrade the
            ubiquitin tag
  JOURNAL   Nat Commun 12 (1), 6173 (2021)
   PUBMED   34702852
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4426)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 4426)
  AUTHORS   Coux,O., Tanaka,K. and Goldberg,A.L.
  TITLE     Structure and functions of the 20S and 26S proteasomes
  JOURNAL   Annu Rev Biochem 65, 801-847 (1996)
   PUBMED   8811196
  REMARK    Review article
REFERENCE   7  (bases 1 to 4426)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   8  (bases 1 to 4426)
  AUTHORS   Nothwang,H.G., Tamura,T., Tanaka,K. and Ichihara,A.
  TITLE     Sequence analyses and inter-species comparisons of three novel
            human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine
            potential proteolytic active-site residues
  JOURNAL   Biochim Biophys Acta 1219 (2), 361-368 (1994)
   PUBMED   7918633
REFERENCE   9  (bases 1 to 4426)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   10 (bases 1 to 4426)
  AUTHORS   Dawson,S.J. and White,L.A.
  TITLE     Treatment of Haemophilus aphrophilus endocarditis with
            ciprofloxacin
  JOURNAL   J Infect 24 (3), 317-320 (1992)
   PUBMED   1602151
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL354864.16, BC101836.1,
            AL157951.5 and BM664618.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002794.4.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the proteasome B-type family, also known as the T1B
            family, that is a 20S core beta subunit. Multiple alternatively
            spliced transcript variants encoding distinct isoforms have been
            found for this gene. [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.4284323.1,
                                           SRR14243140.4470568.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373237.4/ ENSP00000362334.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AL354864.16        172724-172773
            51-733              BC101836.1         1-683
            734-4072            AL157951.5         5907-9245
            4073-4426           BM664618.1         16-369              c
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.3"
     gene            1..4426
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /note="proteasome 20S subunit beta 2"
                     /db_xref="GeneID:5690"
                     /db_xref="HGNC:HGNC:9539"
                     /db_xref="MIM:602175"
     exon            1..185
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..700
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /EC_number="3.4.25.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     macropain subunit C7-I; multicatalytic endopeptidase
                     complex subunit C7-1; proteasome component C7-I;
                     proteasome subunit, beta type, 2; proteasome beta 2
                     subunit; multicatalytic endopeptidase complex subunit
                     C7-I; proteasome subunit beta 2; proteasome subunit beta4;
                     beta-4; proteasome (prosome, macropain) subunit, beta
                     type, 2; testicular tissue protein Li 152; proteasome
                     subunit beta-4"
                     /codon_start=1
                     /product="proteasome subunit beta type-2 isoform 1"
                     /protein_id="NP_002785.1"
                     /db_xref="CCDS:CCDS394.1"
                     /db_xref="GeneID:5690"
                     /db_xref="HGNC:HGNC:9539"
                     /db_xref="MIM:602175"
                     /translation="MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILL
                     LCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNL
                     LLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELL
                     RKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS"
     exon            186..308
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /inference="alignment:Splign:2.1.0"
     exon            309..379
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /inference="alignment:Splign:2.1.0"
     exon            380..542
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /inference="alignment:Splign:2.1.0"
     exon            543..592
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /inference="alignment:Splign:2.1.0"
     exon            593..4426
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /inference="alignment:Splign:2.1.0"
     regulatory      803..808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /note="hexamer: AATAAA"
     polyA_site      829
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /note="major polyA site"
     regulatory      2125..2130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /note="hexamer: ATTAAA"
     polyA_site      2149
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
     regulatory      2361..2366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /note="hexamer: ATTAAA"
     polyA_site      2383
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
     regulatory      4403..4408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
                     /note="hexamer: CATAAA"
     polyA_site      4426
                     /gene="PSMB2"
                     /gene_synonym="HC7-I"
ORIGIN      
        1 aatcttcgct ctgaggtgct gtctcaccgg tgagacctgg aagcgggcga gtctcgtgct
       61 gtgtcggacc tgcagcccct ggccttccgc caccatggag tacctcatcg gtatccaagg
      121 ccccgactat gttcttgtcg cctccgaccg ggtggccgcc agcaatattg tccagatgaa
      181 ggacgatcat gacaagatgt ttaagatgag tgaaaagata ttactcctgt gtgttggaga
      241 ggctggagac actgtacagt ttgcagaata tattcagaaa aacgtgcaac tttataagat
      301 gcgaaatgga tatgaattgt ctcccacggc agcagctaac ttcacacgcc gaaacctggc
      361 tgactgtctt cggagtcgga ccccatatca tgtgaacctc ctcctggctg gctatgatga
      421 gcatgaaggg ccagcgctgt attacatgga ctacctggca gccttggcca aggccccttt
      481 tgcagcccac ggctatggtg ccttcctgac tctcagtatc ctcgaccgat actacacacc
      541 gactatctca cgtgagaggg cagtggaact ccttaggaaa tgtctggagg agctccagaa
      601 acgcttcatc ctgaatctgc caaccttcag tgttcgaatc attgacaaaa atggcatcca
      661 tgacctggat aacatttcct tccccaaaca gggctcctaa catcatgtcc tccctcccac
      721 ttgccaggga actttttttt gatgggctcc tttatttttt tctactcttt tcaggcgcac
      781 tcttgataaa tggttaattc agaataaagg tgactatgga tataattgag ccctctggtc
      841 caggtctcag tttacctaat attacctcag aaaggatatg gagggaagat gatctttttg
      901 ccaggtctga cttttcttcc tgctccgccc tccattaacg ctcagtaccc tttagcagct
      961 gacggcccca cgttctactc catgcttggc ttcctttcca actagctctt tcatatattt
     1021 tacttgctag tatctccatt ctctctaaag tagtggttct ttttgccctt aaacttaaat
     1081 ttttaaatta attaacctga attaataata catgcactta atgtaacatg caaacagtac
     1141 aaaaacatgt agtgaaaaat atttcttcca gagctgggtg tggtggctca tacctgtaat
     1201 cccagcactt tgggaggccg aggcgggcgg atcacgaggt caagagagcg agaccatcct
     1261 gaccaacatg gtaaaacccc gtctctacta aaaatacaaa aattagctgg gcgtggtggc
     1321 acgcacccct agtcccagct actggggagg ctgagacagg agaatcggtc gaacccggga
     1381 ggcagaggtt gcagtgagcc tagatcgcgc cactgtactc cagcctggca acagagtgag
     1441 actccgtctc aaaaaaagaa ggaaaaatgt ttcttccatc cctataatcc agtcatctgc
     1501 ttcctgcttc ccttcctgga ggaaacttca gctactaatt tcttatgttt tttaagagat
     1561 attctgttac tgtgtaaagt atacatacat acagacacat gcccccttta aattttttag
     1621 atttatttat ttatttagag acagggtctc actctagccc aggctggagt gctgtggcgt
     1681 aatcttggct cactgcaacc tccgcctccc gggcccaagt gatcctccca tctcagcctc
     1741 ctgagtagct aggattacag gcgcacacca ccaatgccca gctagttttt gtgtttttca
     1801 tagagacagg gtctcaccat gtcattcagg ttggtcttga actcctgggc tcaagcagtc
     1861 tgcctgcctt ggcttcccag tgctgggatt acaggcgtga gccaccgtgc ccggctaaaa
     1921 agtattttta agttctgcat attgcttatt tcacttaaca ctatattaga gattgtttta
     1981 tatcaataca tatagatatg cttattcttg ttgacagttg cataattttc cattaaattg
     2041 atgtatcatg ggcagttaac cagttactcg ttttactctt agcataactt tagggaacaa
     2101 tgtggatgtt ttgtggttaa agctattaaa acagtggttc ttgaccagat gtgtgtttca
     2161 gaatgttcat ctcacatttc cagttgctac tgatgctgct ggtctaggaa ccacatatca
     2221 aaaccatttg gtctccgact aagataaacc ctactttatc caattctgtg ctcctcttaa
     2281 tatcatatac agataggttt ttgttttgtc tgtgattaaa tgtatcatct aagatactac
     2341 ctactgatca taacgttcat attaaagtat tgggttaaca ttagatttgg gtgtcttata
     2401 cactatttaa tacgccaatg ataaatgcct tacaccataa gaagaaagct aatcattgag
     2461 taaatgggag aaaagagatg attcacgtca ctacagttga ttcaacctcc aacaccacca
     2521 tgaatatgca taggtttgag cttttagcta gattcctgag tgtaaggcat ctattttaaa
     2581 agtgccacag gttggccggg tgcggtggct catgcctgta atcccagcac tttggccgcc
     2641 cgccgaggcg ggcggatcac ctgaggtcag gagttcaaga ccagcctggc caacatggtg
     2701 aaaccccatc tctactaaaa aaatatataa caattagcca ggcgtggtgg cacacgcctg
     2761 taaacccaac tacttgggag gctgaggcag gagaattgct tgagcccggg agagggaggt
     2821 tgcagtgagc cgagatcatg ccactgcact ccagcctggc tgacagagca agactctgtc
     2881 tcaaaaaaaa aaaaaaaaaa aaaaaaaaag cgccacagat gattctgacg ccctacccag
     2941 tggagtgctg ctactaaagc acagactatc ctgctacatg attttcccat ctgtacaatg
     3001 actgaaatag cacctattct atgtggtaga tatgagaatt ccatgaagta atataggtaa
     3061 acatttagtt cagggcttgg catgtggtaa gtgctcagta agtgtataat gattggtaaa
     3121 cttgttattc tgcattcagg cctcaccagg gatgacatac ggttctctgc ctttgaatgt
     3181 aagtgtcccc agggcaatct caggcaagcc tgcaaaccca tcctgactct cagtgttttt
     3241 ttgccaatct ggatctcttt cctgagctcc aaatcaactc attcctagat agctcaccct
     3301 ccagatttag ggacatcgaa ctcagtatgt ctaaagctga gccttaccat ctgcccctct
     3361 ccaaaccgcc tctatgacat tctgtcttag agaattagag ctacctggtt tcccaagtgc
     3421 ccaagtcaga aacccaggca tcatttattt ttaataaatg ttttattttg gaataaattt
     3481 ggttttacag aaaagttgca aagataatat ggtgtcctta taccccttat tcagtttctg
     3541 taatgttaac atcttctata taccacagta tgtttgtcaa aactgagaaa ccaacattgg
     3601 ttggtgtatt agtattacta agctccaggc tttattcaga ttttaccagt ttttccacta
     3661 attttcttct gttctaggat ataatccaag atacaacatt gcatttagct gggcatcatt
     3721 tttatgccat tctttatctc ctgtctctac atggccacca agttctggca tttctacatc
     3781 ctaaatcttt taattttatt ataatttttt attgttgttt ttcagagaca gggtcttgct
     3841 ccatcaccta ggctggagta cagtggcacg atcgtagctt actgcagtaa gttcacttga
     3901 actcctgggc tcaagtgaac ctcttacccc agcctcccaa ctagctggga ctagaggggt
     3961 gcaccactgc acccagctaa ttttgtcaaa attttttgta gagacagggt ctcgccatgt
     4021 tgcctaggct ggtctcgaac tcttggcctc aagtgatcct cccaccttgg tctgccaaag
     4081 cactgggatt acagatgtga gccactgcac ccagcctaat ctttataaat ctccatcccc
     4141 attactctgg ttcaccccct atcgtttctt agactattta acagcctcct aacttcactc
     4201 tacttttaat ctcttccctc tccaggtctt tctccatatt ttaaaaaact agtttcacca
     4261 tccattcctc tctgccctta aaactttctg gtggttttcc atggcttaag agaataaagc
     4321 ccaattcttt gatttgacat gctaggcatt ccataatcct ctccgaacct aactccccac
     4381 ttcctttccc tcctttacac ctcataaagc ctgtgttcca actaaa
//
