LOCUS       NM_002791               1023 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit alpha 6 (PSMA6), transcript
            variant 1, mRNA.
ACCESSION   NM_002791
VERSION     NM_002791.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   de Almeida,M., Hinterndorfer,M., Brunner,H., Grishkovskaya,I.,
            Singh,K., Schleiffer,A., Jude,J., Deswal,S., Kalis,R., Vunjak,M.,
            Lendl,T., Imre,R., Roitinger,E., Neumann,T., Kandolf,S.,
            Schutzbier,M., Mechtler,K., Versteeg,G.A., Haselbach,D. and
            Zuber,J.
  TITLE     AKIRIN2 controls the nuclear import of proteasomes in vertebrates
  JOURNAL   Nature 599 (7885), 491-496 (2021)
   PUBMED   34711951
REFERENCE   3  (bases 1 to 1023)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1023)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1023)
  AUTHORS   Zmorzynski,S., Popek-Marciniec,S., Styk,W., Wojcierowska-Litwin,M.,
            Korszen-Pilecka,I., Szudy-Szczyrek,A., Chocholska,S., Hus,M. and
            Filip,A.A.
  TITLE     The Impact of the NOD2/CARD15 Variant (3020insC) and PSMA6
            Polymorphism (-8C>G) on the Development and Outcome of Multiple
            Myeloma
  JOURNAL   Biomed Res Int 2020, 7629456 (2020)
   PUBMED   32596371
  REMARK    GeneRIF: The Impact of the NOD2/CARD15 Variant (3020insC) and PSMA6
            Polymorphism (-8C>G) on the Development and Outcome of Multiple
            Myeloma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1023)
  AUTHORS   Nederlof,P.M., Wang,H.R. and Baumeister,W.
  TITLE     Nuclear localization signals of human and Thermoplasma proteasomal
            alpha subunits are functional in vitro
  JOURNAL   Proc Natl Acad Sci U S A 92 (26), 12060-12064 (1995)
   PUBMED   8618844
REFERENCE   7  (bases 1 to 1023)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   8  (bases 1 to 1023)
  AUTHORS   Kato,S., Sekine,S., Oh,S.W., Kim,N.S., Umezawa,Y., Abe,N.,
            Yokoyama-Kobayashi,M. and Aoki,T.
  TITLE     Construction of a human full-length cDNA bank
  JOURNAL   Gene 150 (2), 243-250 (1994)
   PUBMED   7821789
REFERENCE   9  (bases 1 to 1023)
  AUTHORS   Bey,F., Silva Pereira,I., Coux,O., Viegas-Pequignot,E., Recillas
            Targa,F., Nothwang,H.G., Dutrillaux,B. and Scherrer,K.
  TITLE     The prosomal RNA-binding protein p27K is a member of the alpha-type
            human prosomal gene family
  JOURNAL   Mol Gen Genet 237 (1-2), 193-205 (1993)
   PUBMED   7681138
REFERENCE   10 (bases 1 to 1023)
  AUTHORS   DeMartino,G.N., Orth,K., McCullough,M.L., Lee,L.W., Munn,T.Z.,
            Moomaw,C.R., Dawson,P.A. and Slaughter,C.A.
  TITLE     The primary structures of four subunits of the human,
            high-molecular-weight proteinase, macropain (proteasome), are
            distinct but homologous
  JOURNAL   Biochim Biophys Acta 1079 (1), 29-38 (1991)
   PUBMED   1888762
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG701535.1, BC002979.1,
            BQ009843.1 and AL133163.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_002791.2.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the peptidase T1A family, that is a 20S core alpha
            subunit. Multiple transcript variants encoding several different
            isoforms have been found for this gene. A pseudogene has been
            identified on the Y chromosome. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.272449.1,
                                           SRR1803611.73024.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261479.9/ ENSP00000261479.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                BG701535.1         48-91
            45-1004             BC002979.1         1-960
            1005-1009           BQ009843.1         18-22               c
            1010-1023           AL133163.3         17269-17282
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q13.2"
     gene            1..1023
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /note="proteasome 20S subunit alpha 6"
                     /db_xref="GeneID:5687"
                     /db_xref="HGNC:HGNC:9535"
                     /db_xref="MIM:602855"
     exon            1..171
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..836
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /EC_number="3.4.25.1"
                     /note="isoform a is encoded by transcript variant 1;
                     macropain subunit iota; prosomal P27K protein; proteasome
                     subunit iota; proteasome iota chain; proteasome subunit
                     alpha 6; proteasome subunit alpha1; macropain iota chain;
                     multicatalytic endopeptidase complex iota chain; 27 kDa
                     prosomal protein; PROS-27; proteasome (prosome, macropain)
                     subunit, alpha type, 6; testicular secretory protein Li
                     44; alpha-1; proteasome subunit alpha-1"
                     /codon_start=1
                     /product="proteasome subunit alpha type-6 isoform a"
                     /protein_id="NP_002782.1"
                     /db_xref="CCDS:CCDS9655.1"
                     /db_xref="GeneID:5687"
                     /db_xref="HGNC:HGNC:9535"
                     /db_xref="MIM:602855"
                     /translation="MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRG
                     KDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWK
                     YKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYY
                     CGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVG
                     VVTVENPKFRILTEAEIDAHLVALAERD"
     exon            172..266
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     exon            267..348
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     exon            349..504
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     exon            505..683
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     exon            684..778
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     exon            779..1023
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      984..989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /note="hexamer: AACAAG"
     polyA_site      1001
                     /gene="PSMA6"
                     /gene_synonym="IOTA; p27K; PROS27"
                     /note="major polyA site"
ORIGIN      
        1 ggaagcagtc gctgcaactt ccgggaggtg cttgtgtgcc tggtgcggga gctacggggc
       61 ccagggattg tgtttaaagt agtgcttcta ccaacatgtc ccgtggttcc agcgccggtt
      121 ttgaccgcca cattaccatt ttttcacccg agggtcggct ctaccaagta gaatatgctt
      181 ttaaggctat taaccagggt ggccttacat cagtagctgt cagagggaaa gactgtgcag
      241 taattgtcac acagaagaaa gtacctgaca aattattgga ttccagcaca gtgactcact
      301 tattcaagat aactgaaaac attggttgtg tgatgaccgg aatgacagct gacagcagat
      361 cccaggtaca gagggcacgc tatgaggcag ctaactggaa atacaagtat ggctatgaga
      421 ttcctgtgga catgctgtgt aaaagaattg ccgatatttc tcaggtctac acacagaatg
      481 ctgaaatgag gcctcttggt tgttgtatga ttttaattgg tatagatgaa gagcaaggcc
      541 ctcaggtata taagtgtgat cctgcaggtt actactgtgg gtttaaagcc actgcagcgg
      601 gagttaaaca aactgagtca accagcttcc ttgaaaaaaa agtgaagaag aaatttgatt
      661 ggacatttga acagacagtg gaaactgcaa ttacatgcct gtctactgtt ctatcaattg
      721 atttcaaacc ttcagaaata gaagttggag tagtgacagt tgaaaatcct aaattcagga
      781 ttcttacaga agcagagatt gatgctcacc ttgttgctct agcagagaga gactaaacat
      841 tgtcgttagt ttaccagatc cgtgatgcca cttacctgtg tgtttggtaa caacaaacca
      901 acatcatgga ggtccctgga ttgaaaaagg agcctctccc actcctccta ccaccgaagt
      961 ggttaggact ctatataaat aaaaacaagg cttttggaaa ataattgcat cctgttgttt
     1021 tca
//
