LOCUS       NM_002789               4378 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit alpha 4 (PSMA4), transcript
            variant 1, mRNA.
ACCESSION   NM_002789
VERSION     NM_002789.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Bai,Y., Zheng,J., Cheng,L., Liu,Q., Zhao,G., Li,J., Gu,Y., Xu,W.,
            Wang,M., Wei,Q. and Zhang,R.
  TITLE     Potentially functional genetic variants of VAV2 and PSMA4 in the
            immune-activation pathway and non-small cell lung cancer survival
  JOURNAL   J Gene Med 24 (10), e3447 (2022)
   PUBMED   36039727
  REMARK    GeneRIF: Potentially functional genetic variants of VAV2 and PSMA4
            in the immune-activation pathway and non-small cell lung cancer
            survival.
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Zhao,J., Makhija,S., Zhou,C., Zhang,H., Wang,Y., Muralidharan,M.,
            Huang,B. and Cheng,Y.
  TITLE     Structural insights into the human PA28-20S proteasome enabled by
            efficient tagging and purification of endogenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 119 (33), e2207200119 (2022)
   PUBMED   35858375
REFERENCE   3  (bases 1 to 4378)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 4378)
  AUTHORS   de Almeida,M., Hinterndorfer,M., Brunner,H., Grishkovskaya,I.,
            Singh,K., Schleiffer,A., Jude,J., Deswal,S., Kalis,R., Vunjak,M.,
            Lendl,T., Imre,R., Roitinger,E., Neumann,T., Kandolf,S.,
            Schutzbier,M., Mechtler,K., Versteeg,G.A., Haselbach,D. and
            Zuber,J.
  TITLE     AKIRIN2 controls the nuclear import of proteasomes in vertebrates
  JOURNAL   Nature 599 (7885), 491-496 (2021)
   PUBMED   34711951
REFERENCE   5  (bases 1 to 4378)
  AUTHORS   Sahu,I., Mali,S.M., Sulkshane,P., Xu,C., Rozenberg,A., Morag,R.,
            Sahoo,M.P., Singh,S.K., Ding,Z., Wang,Y., Day,S., Cong,Y.,
            Kleifeld,O., Brik,A. and Glickman,M.H.
  TITLE     The 20S as a stand-alone proteasome in cells can degrade the
            ubiquitin tag
  JOURNAL   Nat Commun 12 (1), 6173 (2021)
   PUBMED   34702852
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4378)
  AUTHORS   Palmer,A., Rivett,A.J., Thomson,S., Hendil,K.B., Butcher,G.W.,
            Fuertes,G. and Knecht,E.
  TITLE     Subpopulations of proteasomes in rat liver nuclei, microsomes and
            cytosol
  JOURNAL   Biochem J 316 (Pt 2) (Pt 2), 401-407 (1996)
   PUBMED   8687380
REFERENCE   7  (bases 1 to 4378)
  AUTHORS   Castano,J.G., Mahillo,E., Arizti,P. and Arribas,J.
  TITLE     Phosphorylation of C8 and C9 subunits of the multicatalytic
            proteinase by casein kinase II and identification of the C8
            phosphorylation sites by direct mutagenesis
  JOURNAL   Biochemistry 35 (12), 3782-3789 (1996)
   PUBMED   8619999
REFERENCE   8  (bases 1 to 4378)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   9  (bases 1 to 4378)
  AUTHORS   Arribas,J., Arizti,P. and Castano,J.G.
  TITLE     Antibodies against the C2 COOH-terminal region discriminate the
            active and latent forms of the multicatalytic proteinase complex
  JOURNAL   J Biol Chem 269 (17), 12858-12864 (1994)
   PUBMED   8175701
REFERENCE   10 (bases 1 to 4378)
  AUTHORS   Tamura,T., Lee,D.H., Osaka,F., Fujiwara,T., Shin,S., Chung,C.H.,
            Tanaka,K. and Ichihara,A.
  TITLE     Molecular cloning and sequence analysis of cDNAs for five major
            subunits of human proteasomes (multi-catalytic proteinase
            complexes)
  JOURNAL   Biochim Biophys Acta 1089 (1), 95-102 (1991)
   PUBMED   2025653
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG528289.1, BC022445.1,
            BC030529.1, CD365826.1, BG208993.1 and AC027228.16.
            
            On Nov 23, 2018 this sequence version replaced NM_002789.5.
            
            Summary: This gene encodes a core alpha subunit of the 20S
            proteosome, which is a highly ordered ring-shaped structure
            composed of four rings of 28 non-identical subunits. Proteasomes
            cleave peptides in an ATP- and ubiquitin-dependent manner.
            [provided by RefSeq, Aug 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.40060.1,
                                           SRR1803615.115885.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000044462.12/ ENSP00000044462.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               BG528289.1         41-205
            166-688             BC022445.1         137-659
            689-838             BC030529.1         2105-2254
            839-1181            CD365826.1         16-358              c
            1182-1223           BG208993.1         436-477
            1224-4378           AC027228.16        44597-47751         c
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.1"
     gene            1..4378
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /note="proteasome 20S subunit alpha 4"
                     /db_xref="GeneID:5685"
                     /db_xref="HGNC:HGNC:9533"
                     /db_xref="MIM:176846"
     exon            1..96
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            97..122
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..905
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /EC_number="3.4.25.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proteasome component C9; proteasome subunit HC9;
                     proteasome subunit L; macropain subunit C9; alpha-3;
                     multicatalytic endopeptidase complex subunit C9;
                     proteasome subunit alpha type-4; proteasome (prosome,
                     macropain) subunit, alpha type, 4; proteasome subunit
                     alpha 4; proteasome subunit alpha3; proteasome subunit
                     alpha-3"
                     /codon_start=1
                     /product="proteasome subunit alpha type-4 isoform 1"
                     /protein_id="NP_002780.1"
                     /db_xref="CCDS:CCDS10303.1"
                     /db_xref="GeneID:5685"
                     /db_xref="HGNC:HGNC:9533"
                     /db_xref="MIM:176846"
                     /translation="MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVL
                     LAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQ
                     EPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWK
                     ATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTR
                     ENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK"
     exon            123..165
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            166..328
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            329..406
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            407..495
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            496..626
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            627..750
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     exon            751..4378
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1132..1137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /note="hexamer: AATAAA"
     regulatory      1160..1165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /note="hexamer: AATAAA"
     polyA_site      1161
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /note="major polyA site"
     regulatory      1188..1193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /note="hexamer: AATATA"
     polyA_site      1199
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
     polyA_site      1223
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
     regulatory      4360..4365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
                     /note="hexamer: AATAAA"
     polyA_site      4378
                     /gene="PSMA4"
                     /gene_synonym="HC9; HsT17706; PSC9"
ORIGIN      
        1 atattagcag cggttattcg gtgagcggtg gtggtttatt cttccgtgga gttaagggct
       61 ccgtggacat ctcaggtctt cagggtcttc catctggaac tatataaagt tcagaaaaca
      121 tgtctcgaag atatgactcc aggaccacta tattttctcc agaaggtcgc ttataccaag
      181 ttgaatatgc catggaagct attggacatg caggcacctg tttgggaatt ttagcaaatg
      241 atggtgtttt gcttgcagca gagagacgca acatccacaa gcttcttgat gaagtctttt
      301 tttctgaaaa aatttataaa ctcaatgagg acatggcttg cagtgtggca ggcataactt
      361 ctgatgctaa tgttctgact aatgaactaa ggctcattgc tcaaaggtat ttattacagt
      421 atcaggagcc aataccttgt gagcagttgg ttacagcgct gtgtgatatc aaacaagctt
      481 atacacaatt tggaggaaaa cgtccctttg gtgtttcatt gctgtacatt ggctgggata
      541 agcactatgg ctttcagctc tatcagagtg accctagtgg aaattacggg ggatggaagg
      601 ccacatgcat tggaaataat agcgctgcag ctgtgtcaat gttgaaacaa gactataaag
      661 aaggagaaat gaccttgaag tcagcacttg ctttagctat caaagtacta aataagacca
      721 tggatgttag taaactctct gctgaaaaag tggaaattgc aacactaaca agagagaatg
      781 gaaagacagt aatcagagtt ctcaaacaaa aagaagtgga gcagttgatc aaaaaacatg
      841 aggaagaaga agccaaagct gagcgtgaga agaaagaaaa agaacagaaa gaaaaggata
      901 aatagaatca gagattttat tactcatttg gggcaccatt tcagtgtaaa agcagtccta
      961 ctcttccaca ctaggaaggc tttacttttt ttaactggtg cagtgggaaa ataggacatt
     1021 acatactgaa ttgggtcctt gtcatttctg tccaattgaa tactttattg taacgatgat
     1081 ggttaccctt catggacgtc ttaatcttcc acacacatcc cctttttttg gaataaaatt
     1141 tggaaaatgg aaatgaagga ataaattctc tgtagcagta attgttaaat atacaaaaac
     1201 tgacagggcg attacttttt gcacattgtg gcttacagtc ctttcacaaa gatgggcacg
     1261 ttcctcaggc tggccagatt attcgtctct gtctgcagga attggtttga gaatgaacac
     1321 gtgagctgaa caaagccaga attgatgtgt gggtgtggat ctcgcagcta ggactgaggc
     1381 tgcccacagg cagcagctca agctgcgcgg aagatgaggt gtagaagtta aggccacaga
     1441 ggaaaagtgg aaccaagaga tgagaacagt tacgtgttgg ctgaagggag ctctgttgct
     1501 ggggtttgtt caacctgagt tcccttggct atctgtggct tcatgacaga agtgatacag
     1561 ggcagtggta cttaatgcgt agccatggtg ctttagcatc acctatgcag attctgatgt
     1621 cccatcccag atctactgaa ttaaaatctc tgagggtgaa acccagtaat ctgttctaac
     1681 aagctctgca tgtgattctt aggaatgcta agtaaaactt taagaagttt ttgttgcata
     1741 ttagaatcac ctaggaagct tttgcagctt gtaagcatgc tgtatcgatt aactcaagct
     1801 ctgtggtgga acccagatat taacattgct taaactcttc aggtgatttc catcagctag
     1861 ggtgagtgca ttgagccatc atttaccctt ctctcagctg ttgtcaacca aatatgtgct
     1921 cccagtctaa tttctcattg tagattttct taatgcagct ccctgcagtt ttctgtatag
     1981 gctaaagtat ttacaagccc tatttgcagc acttttttga acttctaaat tattctctta
     2041 ctctgtacag caggttctcg ttatttttgg tagttatgtt ctaaagtcac cacaaaaact
     2101 aacaaaatac tgaactcttg cacctagaga aagtactggg ttagggtcct acaagccact
     2161 ggtcacattt ttatcaactg atcagtacat aacctgtttt ttgtgtattt ctacttaaag
     2221 acaccttttt aaatgtgtat tgtggattca ttaacataga actcagccaa cagctcttta
     2281 cgtgaatgtt tttgttttgt tttgtttttt gagactgggt cttgctctgt cacccaggct
     2341 ggagtgcagt ggcgtgatca tatttcactg caggctcaaa cggtcttcct gccttgacct
     2401 cccaaagtgt tgacatcaca gccctcttat gcttaggaac aacactaaac agtgtttttt
     2461 tcatagagac ggtgagcagg gaaggtctca ccatgtttcc catgccggtc tcaagctcct
     2521 ggactcaagc gatcctcctg cctcagcctc ccaaagtgct gacatcacag ccctcttgtg
     2581 cttaggaaca ttaaacagca cttcagcact atacctgggt gccatttgaa atagtaaaat
     2641 cactaagaaa aatgcaaaaa taaacggcac aaggtagact gcaggaacac ttacacggtg
     2701 tgagagctga aacaagaatt cagagtgtcg ccttgctcaa cctcagatgg gaacatgtgc
     2761 acctgggtac tcaaaatttt caccactctg catgtcaaca aatgactaaa agtaccacaa
     2821 gtattggttt gggggttaca tgtacatttt atgagaatgc aaattttgca aattcacaag
     2881 tacagaatca gcaagtagtg aagatgaatg gcacgtcttc accctccagt cttgctacac
     2941 ccagaggttg aggtagtcaa taaatcttat taactctgtc tgcagaattt acccataatc
     3001 cagccacttt ccactgcagg aacgctggtc caattcacca ttatctctga cttggactat
     3061 tgcaacagcc tcctaactgg ttccttgcct ccacttcatt ccccacagtc aattctccag
     3121 cagcagccac agtgatcctt tcaaaatgta aacggtacta ctgcacagtt ttggctcaaa
     3181 accctccaat ggcttcccat cttgctcagt aagagtcaaa ttctttgtac tggttcatga
     3241 ggtatcctgt attctggcct tgtgctacct tccatgtgct tccttcctca ctcattcatc
     3301 caaatgagcc ttctcatcat tccctgaatg tgccaagctc ccctcacatc acttgccaat
     3361 ccctctgcct tgggaatgct cttcctgggt atctattgcc cactccctca tctccaggtc
     3421 tctgcttgca tgtcaccttt tttggggtac ttctgtgaag gagcaatctc tttcccctgc
     3481 acactcacat tttctattcc ctatacatac cctgttgagt ttttctccat agcactcatc
     3541 accagctggc atatttgttt cttgtctctc cccactggaa tacagacttc cagagcagta
     3601 ctttgttttc attcaatagt cgtgtctcgg tacctagaac agaaactggc acgtgttagg
     3661 caacaaataa gtactgatga atgaataaat accctggata tgctggtgtg aaatcctaaa
     3721 aaatacctgc tttccatcca gatcagtagc actggtggat tttctgtatc ctgactaccc
     3781 cttcaacatt tgcctaaagc taggacaact tcttcaaagg caatagtgaa taagagagcc
     3841 tggtatagtt tgccgccaca ttaggaaagt cttcagaatt tgaccctaac agcaggcaaa
     3901 tttcaacgta ttcatctttt tgttgaaata aattcatact gtgaggaaaa gcaaaagata
     3961 ggaggtgagg caaaagctac tctttaactg tgagtttttt ttccttgcga tggggtttgg
     4021 ctgcctagtc tagattctca cgctagcccc actttcgtct gctctgtggc atatttttaa
     4081 cctgagttgc aggacaggtg gtttccagag tgttgcctga tgcatcccaa atgctgcctt
     4141 agcaatacac atctgaacag gggcccatca ctaatagcaa gcatttcatt aatctgagca
     4201 aaactcatgg gttttcacag ggaccctacc agctcagaag cacgttgata taaataagta
     4261 gaaaaccaaa gacagctaag ttttaaatct tgagtcaaaa ctttaaaatc aaagtcaaat
     4321 tgatttcatt ttggtgagct ttcaaatcat gcttttaaaa ataaattatg taaaaaaa
//
