LOCUS       NM_002788                955 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens proteasome 20S subunit alpha 3 (PSMA3), transcript
            variant 1, mRNA.
ACCESSION   NM_002788
VERSION     NM_002788.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Zhao,J., Makhija,S., Zhou,C., Zhang,H., Wang,Y., Muralidharan,M.,
            Huang,B. and Cheng,Y.
  TITLE     Structural insights into the human PA28-20S proteasome enabled by
            efficient tagging and purification of endogenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 119 (33), e2207200119 (2022)
   PUBMED   35858375
REFERENCE   2  (bases 1 to 955)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 955)
  AUTHORS   Liu,J., Shao,J., Zhang,C., Qin,G., Liu,J., Li,M., Wu,P., Zhao,X.
            and Zhang,Y.
  TITLE     Immuno-oncological role of 20S proteasome alpha-subunit 3 in
            aggravating the progression of esophageal squamous cell carcinoma
  JOURNAL   Eur J Immunol 52 (2), 338-351 (2022)
   PUBMED   34755333
  REMARK    GeneRIF: Immuno-oncological role of 20S proteasome alpha-subunit 3
            in aggravating the progression of esophageal squamous cell
            carcinoma.
REFERENCE   4  (bases 1 to 955)
  AUTHORS   de Almeida,M., Hinterndorfer,M., Brunner,H., Grishkovskaya,I.,
            Singh,K., Schleiffer,A., Jude,J., Deswal,S., Kalis,R., Vunjak,M.,
            Lendl,T., Imre,R., Roitinger,E., Neumann,T., Kandolf,S.,
            Schutzbier,M., Mechtler,K., Versteeg,G.A., Haselbach,D. and
            Zuber,J.
  TITLE     AKIRIN2 controls the nuclear import of proteasomes in vertebrates
  JOURNAL   Nature 599 (7885), 491-496 (2021)
   PUBMED   34711951
REFERENCE   5  (bases 1 to 955)
  AUTHORS   Sahu,I., Mali,S.M., Sulkshane,P., Xu,C., Rozenberg,A., Morag,R.,
            Sahoo,M.P., Singh,S.K., Ding,Z., Wang,Y., Day,S., Cong,Y.,
            Kleifeld,O., Brik,A. and Glickman,M.H.
  TITLE     The 20S as a stand-alone proteasome in cells can degrade the
            ubiquitin tag
  JOURNAL   Nat Commun 12 (1), 6173 (2021)
   PUBMED   34702852
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 955)
  AUTHORS   Castano,J.G., Mahillo,E., Arizti,P. and Arribas,J.
  TITLE     Phosphorylation of C8 and C9 subunits of the multicatalytic
            proteinase by casein kinase II and identification of the C8
            phosphorylation sites by direct mutagenesis
  JOURNAL   Biochemistry 35 (12), 3782-3789 (1996)
   PUBMED   8619999
REFERENCE   7  (bases 1 to 955)
  AUTHORS   Coux,O., Tanaka,K. and Goldberg,A.L.
  TITLE     Structure and functions of the 20S and 26S proteasomes
  JOURNAL   Annu Rev Biochem 65, 801-847 (1996)
   PUBMED   8811196
  REMARK    Review article
REFERENCE   8  (bases 1 to 955)
  AUTHORS   Akioka,H., Forsberg,N.E., Ishida,N., Okumura,K., Nogami,M.,
            Taguchi,H., Noda,C. and Tanaka,K.
  TITLE     Isolation and characterization of the HC8 subunit gene of the human
            proteasome
  JOURNAL   Biochem Biophys Res Commun 207 (1), 318-323 (1995)
   PUBMED   7857283
REFERENCE   9  (bases 1 to 955)
  AUTHORS   Kristensen,P., Johnsen,A.H., Uerkvitz,W., Tanaka,K. and Hendil,K.B.
  TITLE     Human proteasome subunits from 2-dimensional gels identified by
            partial sequencing
  JOURNAL   Biochem Biophys Res Commun 205 (3), 1785-1789 (1994)
   PUBMED   7811265
  REMARK    Erratum:[Biochem Biophys Res Commun. 1995 Feb 27;207(3):1059. doi:
            10.1006/bbrc.1995.1294.. PMID: 7864893]
REFERENCE   10 (bases 1 to 955)
  AUTHORS   Tamura,T., Lee,D.H., Osaka,F., Fujiwara,T., Shin,S., Chung,C.H.,
            Tanaka,K. and Ichihara,A.
  TITLE     Molecular cloning and sequence analysis of cDNAs for five major
            subunits of human proteasomes (multi-catalytic proteinase
            complexes)
  JOURNAL   Biochim Biophys Acta 1089 (1), 95-102 (1991)
   PUBMED   2025653
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CF128376.1, D00762.1 and
            BC038990.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002788.3.
            
            Summary: The proteasome is a multicatalytic proteinase complex with
            a highly ordered ring-shaped 20S core structure. The core structure
            is composed of 4 rings of 28 non-identical subunits; 2 rings are
            composed of 7 alpha subunits and 2 rings are composed of 7 beta
            subunits. Proteasomes are distributed throughout eukaryotic cells
            at a high concentration and cleave peptides in an
            ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
            essential function of a modified proteasome, the immunoproteasome,
            is the processing of class I MHC peptides. This gene encodes a
            member of the peptidase T1A family, that is a 20S core alpha
            subunit. Two alternative transcripts encoding different isoforms
            have been identified. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.451352.1,
                                           SRR1163658.270054.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000216455.9/ ENSP00000216455.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                CF128376.1         39-111
            74-911              D00762.1           1-838
            912-955             BC038990.1         877-920
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.1"
     gene            1..955
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /note="proteasome 20S subunit alpha 3"
                     /db_xref="GeneID:5684"
                     /db_xref="HGNC:HGNC:9532"
                     /db_xref="MIM:176843"
     exon            1..99
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..846
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /EC_number="3.4.25.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proteasome subunit C8; macropain subunit C8;
                     multicatalytic endopeptidase complex subunit C8;
                     proteasome subunit alpha type-3; proteasome component C8;
                     proteasome (prosome, macropain) subunit, alpha type, 3;
                     testicular secretory protein Li 43; proteasome subunit
                     alpha 3; proteasome subunit alpha7; alpha-7; proteasome
                     subunit alpha-7"
                     /codon_start=1
                     /product="proteasome subunit alpha type-3 isoform 1"
                     /protein_id="NP_002779.1"
                     /db_xref="CCDS:CCDS9731.1"
                     /db_xref="GeneID:5684"
                     /db_xref="HGNC:HGNC:9532"
                     /db_xref="MIM:176843"
                     /translation="MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKD
                     GVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSN
                     FGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYG
                     YWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVG
                     ELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM"
     exon            100..182
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            183..306
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            307..408
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            409..482
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            483..555
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            556..621
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            622..668
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            669..736
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            737..801
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     exon            802..955
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      936..941
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /note="hexamer: ATTAAA"
     polyA_site      955
                     /gene="PSMA3"
                     /gene_synonym="HC8; PSC3"
                     /note="major polyA site"
ORIGIN      
        1 actagtttgc ggcatcctgt ggtatagggg aagcgctccg ggcctggaat ccctacgcgt
       61 ccctttgggt ttagcacgat gagctcaatc ggcactgggt atgacctgtc agcctctaca
      121 ttctctcctg acggaagagt ttttcaagtt gaatatgcta tgaaggctgt ggaaaatagt
      181 agtacagcta ttggaatcag atgcaaagat ggtgttgtct ttggggtaga aaaattagtc
      241 ctttctaaac tttatgaaga aggttccaac aaaagacttt ttaatgttga tcggcatgtt
      301 ggaatggcag tagcaggttt gttggcagat gctcgttctt tagcagacat agcaagagaa
      361 gaagcttcca acttcagatc taactttggc tacaacattc cactaaaaca tcttgcagac
      421 agagtggcca tgtatgtgca tgcatataca ctctacagtg ctgttagacc ttttggctgc
      481 agtttcatgt tagggtctta cagtgtgaat gacggtgcgc aactctacat gattgaccca
      541 tcaggtgttt catacggtta ttggggctgt gccatcggca aagccaggca agctgcaaag
      601 acggaaatag agaagcttca gatgaaagaa atgacctgcc gtgatatcgt taaagaagtt
      661 gcaaaaataa tttacatagt acatgacgaa gttaaggata aagcttttga actagaactc
      721 agctgggttg gtgaattaac taatggaaga catgaaattg ttccaaaaga tataagagaa
      781 gaagcagaga aatatgctaa ggaatctctg aaggaagaag atgaatcaga tgatgataat
      841 atgtaacatt tactccagca tctattgtat tttaaatttc tactccagtc caatgtaact
      901 atttagccct ggattataca tactgtccaa ttttcattaa atttttgtct tataa
//
