LOCUS       NM_033222               3342 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens PC4 and SRSF1 interacting protein 1 (PSIP1),
            transcript variant 2, mRNA.
ACCESSION   NM_033222
VERSION     NM_033222.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Jayakumar,S., Patel,M., Boulet,F., Aziz,H., Brooke,G.N., Tummala,H.
            and Pradeepa,M.M.
  TITLE     PSIP1/LEDGF reduces R-loops at transcription sites to maintain
            genome integrity
  JOURNAL   Nat Commun 15 (1), 361 (2024)
   PUBMED   38191578
  REMARK    GeneRIF: PSIP1/LEDGF reduces R-loops at transcription sites to
            maintain genome integrity.
            Erratum:[Nat Commun. 2024 Jun 7;15(1):4865. doi:
            10.1038/s41467-024-49334-6.. PMID: 38849416]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Huo,J., Wang,Y., Zhang,Y., Wang,W., Yang,P., Zhao,W., Zhang,M.,
            Cui,L. and Zhang,D.
  TITLE     The LncRNA MIR155HG is Upregulated by SP1 in Melanoma Cells and
            Drives Melanoma Progression via Modulating the MiR-485-3p/PSIP1
            Axis
  JOURNAL   Anticancer Agents Med Chem 22 (1), 152-159 (2022)
   PUBMED   34225636
  REMARK    GeneRIF: The LncRNA MIR155HG is Upregulated by SP1 in Melanoma
            Cells and Drives Melanoma Progression via Modulating the
            MiR-485-3p/PSIP1 Axis.
REFERENCE   3  (bases 1 to 3342)
  AUTHORS   Lapaillerie,D., Lelandais,B., Mauro,E., Lagadec,F., Tumiotto,C.,
            Miskey,C., Ferran,G., Kuschner,N., Calmels,C., Metifiot,M.,
            Rooryck,C., Ivics,Z., Ruff,M., Zimmer,C., Lesbats,P., Toutain,J.
            and Parissi,V.
  TITLE     Modulation of the intrinsic chromatin binding property of HIV-1
            integrase by LEDGF/p75
  JOURNAL   Nucleic Acids Res 49 (19), 11241-11256 (2021)
   PUBMED   34634812
  REMARK    GeneRIF: Modulation of the intrinsic chromatin binding property of
            HIV-1 integrase by LEDGF/p75.
REFERENCE   4  (bases 1 to 3342)
  AUTHORS   Ortiz-Hernandez,G.L., Sanchez-Hernandez,E.S., Ochoa,P.T.,
            Elix,C.C., Alkashgari,H.R., McMullen,J.R.W., Soto,U.,
            Martinez,S.R., Diaz Osterman,C.J., Mahler,M., Roy,S. and
            Casiano,C.A.
  TITLE     The LEDGF/p75 Integrase Binding Domain Interactome Contributes to
            the Survival, Clonogenicity, and Tumorsphere Formation of
            Docetaxel-Resistant Prostate Cancer Cells
  JOURNAL   Cells 10 (10), 2723 (2021)
   PUBMED   34685704
  REMARK    GeneRIF: The LEDGF/p75 Integrase Binding Domain Interactome
            Contributes to the Survival, Clonogenicity, and Tumorsphere
            Formation of Docetaxel-Resistant Prostate Cancer Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3342)
  AUTHORS   Liedtke,V., Schroder,C., Roggenbuck,D., Weiss,R., Stohwasser,R.,
            Schierack,P., Rodiger,S. and Schenk,L.
  TITLE     LEDGF/p75 Is Required for an Efficient DNA Damage Response
  JOURNAL   Int J Mol Sci 22 (11), 5866 (2021)
   PUBMED   34070855
  REMARK    GeneRIF: LEDGF/p75 Is Required for an Efficient DNA Damage
            Response.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3342)
  AUTHORS   Ochs,R.L., Muro,Y., Si,Y., Ge,H., Chan,E.K. and Tan,E.M.
  TITLE     Autoantibodies to DFS 70 kd/transcription coactivator p75 in atopic
            dermatitis and other conditions
  JOURNAL   J Allergy Clin Immunol 105 (6 Pt 1), 1211-1220 (2000)
   PUBMED   10856157
REFERENCE   7  (bases 1 to 3342)
  AUTHORS   Singh,D.P., Kimura,A., Chylack,L.T. Jr. and Shinohara,T.
  TITLE     Lens epithelium-derived growth factor (LEDGF/p75) and p52 are
            derived from a single gene by alternative splicing
  JOURNAL   Gene 242 (1-2), 265-273 (2000)
   PUBMED   10721720
REFERENCE   8  (bases 1 to 3342)
  AUTHORS   Singh,D.P., Ohguro,N., Kikuchi,T., Sueno,T., Reddy,V.N., Yuge,K.,
            Chylack,L.T. Jr. and Shinohara,T.
  TITLE     Lens epithelium-derived growth factor: effects on growth and
            survival of lens epithelial cells, keratinocytes, and fibroblasts
  JOURNAL   Biochem Biophys Res Commun 267 (1), 373-381 (2000)
   PUBMED   10623627
REFERENCE   9  (bases 1 to 3342)
  AUTHORS   Ge,H., Si,Y. and Wolffe,A.P.
  TITLE     A novel transcriptional coactivator, p52, functionally interacts
            with the essential splicing factor ASF/SF2
  JOURNAL   Mol Cell 2 (6), 751-759 (1998)
   PUBMED   9885563
  REMARK    GeneRIF: PSIP1/p52 is expressed from the same gene as
            PSIP2/LEDGF/p75 due to alternative splicing of the pre-mRNA. It has
            transcription co-activator activity in vitro and can functionally
            interact with the ASF/SF2 splicing factor in vitro.
REFERENCE   10 (bases 1 to 3342)
  AUTHORS   Ge,H., Si,Y. and Roeder,R.G.
  TITLE     Isolation of cDNAs encoding novel transcription coactivators p52
            and p75 reveals an alternate regulatory mechanism of
            transcriptional activation
  JOURNAL   EMBO J 17 (22), 6723-6729 (1998)
   PUBMED   9822615
  REMARK    GeneRIF: PSIP2/LEDGF/p75 co-purifies with transcription coactivator
            PC4 from HeLa cell extract. PSIP2 and PSIP1 interact with VP16
            activation domain and several components of the general
            transcription machinery in vitro.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF063020.1 and AL441925.16.
            
            On Nov 22, 2018 this sequence version replaced NM_033222.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR,
            and 3' coding region, compared to variant 1. This results in a
            protein (isoform 2) with a longer, distinct C-terminus, compared to
            isoform 1. Variants 2 and 3 encode the same protein (isoform 2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF063020.1, SRR14038195.2189834.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380733.9/ ENSP00000370109.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-291               AF063020.1         15-305
            292-1957            AF063020.1         307-1972
            1958-3309           AL441925.16        54105-55456         c
            3310-3342           AF063020.1         3326-3358
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p22.3"
     gene            1..3342
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /note="PC4 and SRSF1 interacting protein 1"
                     /db_xref="GeneID:11168"
                     /db_xref="HGNC:HGNC:9527"
                     /db_xref="MIM:603620"
     exon            1..154
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            155..367
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             296..1888
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     transcriptional coactivator p52/p75; PC4 and
                     SFRS1-interacting protein; PC4 and SFRS1 interacting
                     protein 1; CLL-associated antigen KW-7; dense fine
                     speckles 70 kDa protein; lens epithelium-derived growth
                     factor"
                     /codon_start=1
                     /product="PC4 and SFRS1-interacting protein isoform 2"
                     /protein_id="NP_150091.2"
                     /db_xref="CCDS:CCDS6479.1"
                     /db_xref="GeneID:11168"
                     /db_xref="HGNC:HGNC:9527"
                     /db_xref="MIM:603620"
                     /translation="MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFF
                     FGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQS
                     NASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETE
                     EAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSK
                     KKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSE
                     EEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKK
                     PEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQK
                     HTEMITTLKKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQH
                     EEANKTKDQGKKGPNKKLEKEQTGSKTLNGGSDAQDGNQPQHNGESNEDSKDNHEAST
                     KKKPSSEERETEISLKDSTLDN"
     exon            368..444
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            445..583
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            584..688
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            689..751
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..848
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            849..924
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1153
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1272
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1328
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1399
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1501
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1715
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1827
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..3342
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3321..3326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /note="hexamer: ATTAAA"
     polyA_site      3342
                     /gene="PSIP1"
                     /gene_synonym="DFS70; LEDGF; p52; p75; PAIP; PSIP2"
                     /note="major polyA site"
ORIGIN      
        1 agcagcgcag ctacggcggc ggcagcggcg gcgcggttgc gattccgagc cgttgagacg
       61 cctctgcggc agctggtggc gcaggtggct tgcgtggacg cgggtagagg cgaccggcca
      121 gcaaccgcag cgtcggcgcc cgcggccccg gcagcaggcg cgtcgggacg ccccgaggca
      181 tcctcccccg cccgcgggcc cggtagctgg gcccgcgtcc gccgcccgca tccccgcgcc
      241 gccgcatctc ctcgccgcct cccgggcttc ggacccccgg tctcgccccc gaaacatgac
      301 tcgcgatttc aaacctggag acctcatctt cgccaagatg aaaggttatc cccattggcc
      361 agctcgagta gacgaagttc ctgatggagc tgtaaagcca cccacaaaca aactacccat
      421 tttctttttt ggaactcatg agactgcttt tttaggacca aaggatatat ttccttactc
      481 agaaaataag gaaaagtatg gcaaaccaaa taaaagaaaa ggttttaatg aaggtttatg
      541 ggagatagat aacaatccaa aagtgaaatt ttcaagtcaa caggcagcaa ctaaacaatc
      601 aaatgcatca tctgatgttg aagttgaaga aaaggaaact agtgtttcaa aggaagatac
      661 cgaccatgaa gaaaaagcca gcaatgagga tgtgactaaa gcagttgaca taactactcc
      721 aaaagctgcc agaaggggga gaaagagaaa ggcagaaaaa caagtagaaa ctgaggaggc
      781 aggagtagtg acaacagcaa cagcatctgt taatctaaaa gtgagtccta aaagaggacg
      841 acctgcagct acagaagtca agattccaaa accaagaggc agacccaaaa tggtaaaaca
      901 gccctgtcct tcagagagtg acatcattac tgaagaggac aaaagtaaga aaaaggggca
      961 agaggaaaaa caacctaaaa agcagcctaa gaaggatgaa gagggccaga aggaagaaga
     1021 taagccaaga aaagagccgg ataaaaaaga ggggaagaaa gaagttgaat caaaaaggaa
     1081 aaatttagct aaaacagggg ttacttcaac ctccgattct gaagaagaag gagatgatca
     1141 agaaggtgaa aagaagagaa aaggtgggag gaactttcag actgctcaca gaaggaatat
     1201 gctgaaaggc caacatgaga aagaagcagc agatcgaaaa cgcaagcaag aggaacaaat
     1261 ggaaactgag cagcagaata aagatgaagg aaagaagcca gaagttaaga aagtggagaa
     1321 gaagcgagaa acatcaatgg attctcgact tcaaaggata catgctgaga ttaaaaattc
     1381 actcaaaatt gataatcttg atgtgaacag atgcattgag gccttggatg aacttgcttc
     1441 acttcaggtc acaatgcaac aagctcagaa acacacagag atgattacta cactgaaaaa
     1501 aatacggcga ttcaaagtta gtcaggtaat catggaaaag tctacaatgt tgtataacaa
     1561 gtttaagaac atgttcttgg ttggtgaagg agattccgtg atcacccaag tgctgaataa
     1621 atctcttgct gaacaaagac agcatgagga agcgaataaa accaaagatc aagggaagaa
     1681 agggccaaac aaaaagctag agaaggaaca aacagggtca aagactctaa atggaggatc
     1741 tgatgctcaa gatggtaatc agccacaaca taacggggag agcaatgaag acagcaaaga
     1801 caaccatgaa gccagcacga agaaaaagcc atccagtgaa gagagagaga ctgaaatatc
     1861 tctgaaggat tctacactag ataactaggt tgacatacct ggaatataga gaacacttga
     1921 gaagtttgta atggttttca tttgaaatag actgctgaaa gttttaaatt tttataagca
     1981 taggtttgat gttgaaaact tgttttgagg gagaaaatcc ctttgtttta aagtaaagta
     2041 aacattatcg ctaagtgtac ttgtgcagta ttaacagcta cattatacag taaatgtggg
     2101 ataaaatcca tttagaaaat gttaaactgc ttttccagac atggttgtag catattttca
     2161 attagtgtgt gtatgttaat gtgtaattga tagtagaaca aagttacatt tttaaaactg
     2221 ctacttgtat aaaccttgcc tcttttccca aatactgtgg gttttgtgca tagtttttac
     2281 aaaccttgga tttaccagac tgtcttttca ctgtttgtgg gttttgtaga agttacacat
     2341 ttttatggta gataaaatgt tacttctata caagtactca ctcccttttt atcaaaagtt
     2401 aattttaatc tcacagtcta cattgtgcta cattatccag cttctttgga acaatgtgtg
     2461 ctctgtatgg ttttttttgg tatgacaact aattaagcaa ctgacatgga actgagaatt
     2521 ctacaaacta taaaacatta atttttgaag gtaatttagt tttgtggctg ggcattcagt
     2581 gaagtcttag gacttctttg cagacaactg actgggtata tataggaatg aatctggctt
     2641 tagggttaaa tcatttaagg tccttttata ggcaggcact agtaactaaa actgaaaact
     2701 aagtaagttt atttttgagg aatgttgtta aaaatgtctt taggaagtca ctaaaactta
     2761 attggaagaa aaaatcatga tgcttataca ataaatatga ataaatgtta tataaggaaa
     2821 ctcacctatt tgaaatcatg gctatattgt ttttattttc tagattccaa aaatacaaac
     2881 actagttgtt ccagcattgt actttgataa gtctgtacat tgacgtgtat ggactaaatc
     2941 cagggtaaaa tcaatgttac aaaatttaag ggtatgttaa ctaaaggata gcatttctaa
     3001 gatattttga atattagggt catttggcac ttctcagcaa gtaggatact tctcatgttt
     3061 tgaaattata tgaatatgga aaaaaatggc ttaagaccag cgtctctgta tgacattgtg
     3121 tggttgaccc tctgagataa ctgttttcat ctacagaatt gcatttttgc ttttaaagag
     3181 gtcttataat ggaactagga atcaccgttt tgagagaacc tgcatatata ccagtcatta
     3241 tctgtttggt ccttatacag ttttaactta cttagattta ttctagttaa gccataagtt
     3301 caacgtgtaa acttgttttc attaaagaat ttttctatca aa
//
