LOCUS       NM_000021               6018 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens presenilin 1 (PSEN1), transcript variant 1, mRNA.
ACCESSION   NM_000021
VERSION     NM_000021.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Parvand,M., Liang,J.J.H., Bozorgmehr,T., Born,D., Luna Cortes,A.
            and Rankin,C.H.
  TITLE     A familial Alzheimer's disease associated mutation in presenilin-1
            mediates amyloid-beta independent cell specific neurodegeneration
  JOURNAL   PLoS One 19 (9), e0289435 (2024)
   PUBMED   39240956
  REMARK    GeneRIF: A familial Alzheimer's disease associated mutation in
            presenilin-1 mediates amyloid-beta independent cell specific
            neurodegeneration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Acosta-Uribe,J., Villegas-Lanau,A., Vallejo,D., Ramirez-Aguilar,L.,
            Solano,J.M., Mejia-Cupajita,B., Aguillon,D., Moreno,S.,
            Mendez,L.G., Baena,A., Madrigal,L., Bocanegra,Y., Quiroz,Y.T.,
            Garcia,G.P., Vasquez,D., Arbelaez,A., Lopera,F., Beach,T.G.,
            Kosik,K.S., White,C.L. 3rd and Giraldo-Chica,M.
  TITLE     Primary lateral sclerosis associated with PSEN1 Pro284Leu variant
            in a Colombian family: Clinical and neuropathological features
  JOURNAL   Alzheimers Dement 20 (9), 6384-6394 (2024)
   PUBMED   39072908
  REMARK    GeneRIF: Primary lateral sclerosis associated with PSEN1 Pro284Leu
            variant in a Colombian family: Clinical and neuropathological
            features.
REFERENCE   3  (bases 1 to 6018)
  AUTHORS   Sun,Y., Islam,S., Gao,Y., Nakamura,T., Tomita,T., Michikawa,M. and
            Zou,K.
  TITLE     Presenilin deficiency enhances tau phosphorylation and its
            secretion
  JOURNAL   J Neurochem 168 (9), 2956-2973 (2024)
   PUBMED   38946496
  REMARK    GeneRIF: Presenilin deficiency enhances tau phosphorylation and its
            secretion.
REFERENCE   4  (bases 1 to 6018)
  AUTHORS   Quiroz,Y.T., Aguillon,D., Aguirre-Acevedo,D.C., Vasquez,D.,
            Zuluaga,Y., Baena,A.Y., Madrigal,L., Hincapie,L., Sanchez,J.S.,
            Langella,S., Posada-Duque,R., Littau,J.L., Villalba-Moreno,N.D.,
            Vila-Castelar,C., Ramirez Gomez,L., Garcia,G., Kaplan,E., Rassi
            Vargas,S., Ossa,J.A., Valderrama-Carmona,P., Perez-Corredor,P.,
            Krasemann,S., Glatzel,M., Kosik,K.S., Johnson,K., Sperling,R.A.,
            Reiman,E.M., Sepulveda-Falla,D., Lopera,F. and
            Arboleda-Velasquez,J.F.
  TITLE     APOE3 Christchurch Heterozygosity and Autosomal Dominant
            Alzheimer's Disease
  JOURNAL   N Engl J Med 390 (23), 2156-2164 (2024)
   PUBMED   38899694
  REMARK    GeneRIF: APOE3 Christchurch Heterozygosity and Autosomal Dominant
            Alzheimer's Disease.
REFERENCE   5  (bases 1 to 6018)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
REFERENCE   6  (bases 1 to 6018)
  AUTHORS   Bird,T.D.
  TITLE     Early-Onset Familial Alzheimer Disease - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301414
REFERENCE   7  (bases 1 to 6018)
  AUTHORS   Bird,T.D.
  TITLE     Alzheimer Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301340
REFERENCE   8  (bases 1 to 6018)
  AUTHORS   St George-Hyslop,P., Haines,J., Rogaev,E., Mortilla,M., Vaula,G.,
            Pericak-Vance,M., Foncin,J.F., Montesi,M., Bruni,A., Sorbi,S.,
            Rainero,I., Pinessi,L., Pollen,D., Polinsky,R., Nee,L., Kennedy,J.,
            Macciardi,F., Rogaeva,E., Liang,Y., Alexandrova,N., Lukiw,W.,
            Schlumpf,K., Tanzi,R., Tsuda,T., Farrer,L., Cantu,J.M., Duara,R.,
            Amaducci,L., Bergamini,L., Gusella,J., Roses,A., Crapper
            McLachlan,D. et al.
  TITLE     Genetic evidence for a novel familial Alzheimer's disease locus on
            chromosome 14
  JOURNAL   Nat Genet 2 (4), 330-334 (1992)
   PUBMED   1303289
REFERENCE   9  (bases 1 to 6018)
  AUTHORS   Schellenberg,G.D., Bird,T.D., Wijsman,E.M., Orr,H.T., Anderson,L.,
            Nemens,E., White,J.A., Bonnycastle,L., Weber,J.L., Alonso,M.E. et
            al.
  TITLE     Genetic linkage evidence for a familial Alzheimer's disease locus
            on chromosome 14
  JOURNAL   Science 258 (5082), 668-671 (1992)
   PUBMED   1411576
REFERENCE   10 (bases 1 to 6018)
  AUTHORS   Schellenberg,G.D., Boehnke,M., Wijsman,E.M., Moore,D.K.,
            Martin,G.M. and Bird,T.D.
  TITLE     Genetic association and linkage analysis of the apolipoprotein CII
            locus and familial Alzheimer's disease
  JOURNAL   Ann Neurol 31 (2), 223-227 (1992)
   PUBMED   1349467
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB203403.1, AK312531.1,
            BC011729.2, DB075605.1, AK094186.1, DA670846.1 and AW069286.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 20, 2018 this sequence version replaced NM_000021.3.
            
            Summary: Alzheimer's disease (AD) patients with an inherited form
            of the disease carry mutations in the presenilin proteins (PSEN1;
            PSEN2) or in the amyloid precursor protein (APP). These
            disease-linked mutations result in increased production of the
            longer form of amyloid-beta (main component of amyloid deposits
            found in AD brains). Presenilins are postulated to regulate APP
            processing through their effects on gamma-secretase, an enzyme that
            cleaves APP. Also, it is thought that the presenilins are involved
            in the cleavage of the Notch receptor, such that they either
            directly regulate gamma-secretase activity or themselves are
            protease enzymes. Several alternatively spliced transcript variants
            encoding different isoforms have been identified for this gene, the
            full-length nature of only some have been determined. [provided by
            RefSeq, Aug 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (I-467).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.202632.1,
                                           SRR1803613.16557.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324501.10/ ENSP00000326366.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               DB203403.1         73-359
            288-1616            AK312531.1         344-1672
            1617-2729           BC011729.2         1658-2770
            2730-3204           DB075605.1         111-585
            3205-3930           AK094186.1         466-1191
            3931-4506           DA670846.1         8-583
            4507-6013           AK094186.1         1768-3274
            6014-6018           AW069286.1         18-22               c
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.2"
     gene            1..6018
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /note="presenilin 1"
                     /db_xref="GeneID:5663"
                     /db_xref="HGNC:HGNC:9508"
                     /db_xref="MIM:104311"
     exon            1..77
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            78..159
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            160..299
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..1616
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /EC_number="3.4.23.-"
                     /note="isoform I-467 is encoded by transcript variant 1;
                     familial Alzheimer Disease"
                     /codon_start=1
                     /product="presenilin-1 isoform I-467"
                     /protein_id="NP_000012.1"
                     /db_xref="CCDS:CCDS9812.1"
                     /db_xref="GeneID:5663"
                     /db_xref="HGNC:HGNC:9508"
                     /db_xref="MIM:104311"
                     /translation="MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSL
                     GHPEPLSNGRPQGNSRQVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKS
                     VSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYK
                     VIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGP
                     LRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQ
                     ERNETLFPALIYSSTMVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDG
                     GFSEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVL
                     VGKASATASGDWNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATD
                     YLVQPFMDQLAFHQFYI"
     exon            300..550
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            551..692
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            693..760
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            761..981
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1080
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1167
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1341
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1460
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..6018
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2709..2714
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /note="hexamer: AGTAAA"
     polyA_site      2729
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /note="major polyA site"
     regulatory      5998..6003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /note="hexamer: AATAAA"
     polyA_site      6018
                     /gene="PSEN1"
                     /gene_synonym="ACNINV3; AD3; CMD1U; FAD; PS-1; PS1; PSNL1;
                     S182"
                     /note="major polyA site"
ORIGIN      
        1 ggaaacaaaa cagcggctgg tctggaagga acctgagcta cgagccgcgg cggcagcggg
       61 gcggcgggga agcgtatacc taatctggga gcctgcaagt gacaacagcc tttgcggtcc
      121 ttagacagct tggcctggag gagaacacat gaaagaaaga acctcaagag gctttgtttt
      181 ctgtgaaaca gtatttctat acagttgctc caatgacaga gttacctgca ccgttgtcct
      241 acttccagaa tgcacagatg tctgaggaca accacctgag caatactgta cgtagccaga
      301 atgacaatag agaacggcag gagcacaacg acagacggag ccttggccac cctgagccat
      361 tatctaatgg acgaccccag ggtaactccc ggcaggtggt ggagcaagat gaggaagaag
      421 atgaggagct gacattgaaa tatggcgcca agcatgtgat catgctcttt gtccctgtga
      481 ctctctgcat ggtggtggtc gtggctacca ttaagtcagt cagcttttat acccggaagg
      541 atgggcagct aatctatacc ccattcacag aagataccga gactgtgggc cagagagccc
      601 tgcactcaat tctgaatgct gccatcatga tcagtgtcat tgttgtcatg actatcctcc
      661 tggtggttct gtataaatac aggtgctata aggtcatcca tgcctggctt attatatcat
      721 ctctattgtt gctgttcttt ttttcattca tttacttggg ggaagtgttt aaaacctata
      781 acgttgctgt ggactacatt actgttgcac tcctgatctg gaattttggt gtggtgggaa
      841 tgatttccat tcactggaaa ggtccacttc gactccagca ggcatatctc attatgatta
      901 gtgccctcat ggccctggtg tttatcaagt acctccctga atggactgcg tggctcatct
      961 tggctgtgat ttcagtatat gatttagtgg ctgttttgtg tccgaaaggt ccacttcgta
     1021 tgctggttga aacagctcag gagagaaatg aaacgctttt tccagctctc atttactcct
     1081 caacaatggt gtggttggtg aatatggcag aaggagaccc ggaagctcaa aggagagtat
     1141 ccaaaaattc caagtataat gcagaaagca cagaaaggga gtcacaagac actgttgcag
     1201 agaatgatga tggcgggttc agtgaggaat gggaagccca gagggacagt catctagggc
     1261 ctcatcgctc tacacctgag tcacgagctg ctgtccagga actttccagc agtatcctcg
     1321 ctggtgaaga cccagaggaa aggggagtaa aacttggatt gggagatttc attttctaca
     1381 gtgttctggt tggtaaagcc tcagcaacag ccagtggaga ctggaacaca accatagcct
     1441 gtttcgtagc catattaatt ggtttgtgcc ttacattatt actccttgcc attttcaaga
     1501 aagcattgcc agctcttcca atctccatca cctttgggct tgttttctac tttgccacag
     1561 attatcttgt acagcctttt atggaccaat tagcattcca tcaattttat atctagcata
     1621 tttgcggtta gaatcccatg gatgtttctt ctttgactat aacaaaatct ggggaggaca
     1681 aaggtgattt tcctgtgtcc acatctaaca aagtcaagat tcccggctgg acttttgcag
     1741 cttccttcca agtcttcctg accaccttgc actattggac tttggaagga ggtgcctata
     1801 gaaaacgatt ttgaacatac ttcatcgcag tggactgtgt ccctcggtgc agaaactacc
     1861 agatttgagg gacgaggtca aggagatatg ataggcccgg aagttgctgt gccccatcag
     1921 cagcttgacg cgtggtcaca ggacgatttc actgacactg cgaactctca ggactaccgt
     1981 taccaagagg ttaggtgaag tggtttaaac caaacggaac tcttcatctt aaactacacg
     2041 ttgaaaatca acccaataat tctgtattaa ctgaattctg aacttttcag gaggtactgt
     2101 gaggaagagc aggcaccagc agcagaatgg ggaatggaga ggtgggcagg ggttccagct
     2161 tccctttgat tttttgctgc agactcatcc tttttaaatg agacttgttt tcccctctct
     2221 ttgagtcaag tcaaatatgt agattgcctt tggcaattct tcttctcaag cactgacact
     2281 cattaccgtc tgtgattgcc atttcttccc aaggccagtc tgaacctgag gttgctttat
     2341 cctaaaagtt ttaacctcag gttccaaatt cagtaaattt tggaaacagt acagctattt
     2401 ctcatcaatt ctctatcatg ttgaagtcaa atttggattt tccaccaaat tctgaatttg
     2461 tagacatact tgtacgctca cttgccccag atgcctcctc tgtcctcatt cttctctccc
     2521 acacaagcag tctttttcta cagccagtaa ggcagctctg tcgtggtagc agatggtccc
     2581 attattctag ggtcttactc tttgtatgat gaaaagaatg tgttatgaat cggtgctgtc
     2641 agccctgctg tcagaccttc ttccacagca aatgagatgt atgcccaaag acggtagaat
     2701 taaagaagag taaaatggct gttgaagcac tttctgtcct ggtattttgt ttttgctttt
     2761 gccacacagt agctcagaat ttgaacaaat agccaaaagc tggtggttga tgaattatga
     2821 actagttgta tcaacacaaa gcaagagttg gggaaagcca tatttaactt ggtgagctgt
     2881 gggagaacct ggtggcagaa ggagaaccaa ctgccaaggg gaaagagaag gggcctccag
     2941 cagcgaaggg gatacagtga gctaatgatg tcaaggagga gtttcaggtt attctcgtca
     3001 gctccacaaa tgggtgcttt gtggtctctg cccgcgttac ctttcctctc aatgtacctt
     3061 tgtgtgaact gggcagtgga ggtgcctgct gcagttacca tggagttcag gctctgggca
     3121 gctcagtcag gcaaaacaca caaacagcca tcagcctgtg tgggctcagg gcacctctgg
     3181 acaaaggctt gtggggcata accttcttta ccacagagag cccttagcta tgctgatcag
     3241 accgtaagcg tttatgagaa acttagtttc ctcctgtggc tgaggagggg ccagcttttt
     3301 cttcttttgc ctgctgtttt ctctcccaat ctatgatatg atatgacctg gtttggggct
     3361 gtctttggtg tttagaatat ttgttttctg tcccaggata tttcttataa gaacctaact
     3421 tcaagagtag tgtgcgagta ctgatctgaa tttaaattaa aattggctta tattaggcag
     3481 tcacagacag gaaaaataag agctatgcaa agaaaggggg atttaaagta gtaggttcta
     3541 tcatctcaat tcattttttt ccatgaaatc ccttcttcca agattcattc cctctctcag
     3601 acatgtgcta gcatgggtat tatcattgag aaagcacagc tacagcaaag ccacctgaat
     3661 agcaatttgt gattggaagc attcttgagg gatccctaat ctagagtaat ttatttgtgt
     3721 aaggatccca aatgtgttgc acctttcatg atacatttct tctctgaaga gggtacgtgg
     3781 ggtgtgtgta tttaaatcca tcctatgtat tactgattgt cctgtgtaga aagatggcaa
     3841 ttattctgtc tctttctcca agtttgagcc acatctcagc cacattgtta gacagtgtac
     3901 agagaaccta tctttccttt tttttttttt aaaggacagg attttgctgt gttgcccagg
     3961 ctagacttga actcctgggc tcaagtaatc cacctcagcc tgagtagctg agactacagc
     4021 ccatcttatt tctttaaatc attcatctca ggcagagaac ttttccctca aacattcttt
     4081 ttagaattag ttcagtcatt cctaaaacat ccaaatgcta gtcttccacc atgaaaaata
     4141 gattgtcact ggaaagaaca gtagcaattt ccataaggat gtgccttcac tcacacggga
     4201 caggcggtgg ttatagagtc gggcaaaacc agcagtagag tatgaccagc caagccaatc
     4261 tgcttaataa aaagatggaa gacagtaagg aaggaaagta gccactaaga gtctgagtct
     4321 gactgggcta cagaataaag ggtatttatg gacagaatgt cattacatgc ctatgggaat
     4381 accaatcata tttggaagat ttgcagattt tttttcagag aggaaagact caccttcctg
     4441 tttttggttc tcagtaggtt cgtgtgtgtt cctagaatca cagctctgac tccaaatgac
     4501 tcaatttctc aattagaaaa agtagaagct ttctaagcaa cttggaagaa aacagtcata
     4561 agtaagcaat ttgttgattt tactacagaa gcaacaactg aagaggcagt gtttttactt
     4621 tcagactccg ggattcccat tctgtagtct ctctgctttt aaaaaccctc cttttgcaat
     4681 agatgcccaa acagatgatg tttattactt gttatttacg tggcctcaga cagtgtatgt
     4741 attctcgata taacttgtag agtgtgaaat ataagtttaa ctaccaaata aggtctccca
     4801 gggttagatg actgcgggaa gcctttgatc ccaaccccca aggctttgta tatttgatca
     4861 tttgtgatct aaccctggaa gaaaaagagc tcagaaacca ctatgaaaaa atttgttcag
     4921 tgttttctgt gttcccgtag gttctggagt ctgaggatgc aaagatgaat aagataaatt
     4981 ctcagaatgt agttataatc tcttgttttc tggtatatgc catctttctt taacttctct
     5041 aaaatattgg gtatttgtca aataaccact tttaacagtt accattactg agggcttata
     5101 cattggtgtt ataaaagtga cttgattcag aaatcaatcc attcagtaaa gtactccttc
     5161 tctaaatttg ctgttatgtc tataaggaac agtttgacct gcccttctcc tcacctcctc
     5221 acctgccttc caacattgaa tttggaagga gacgtgaaaa ttggacattt ggttttgccc
     5281 ttgggctgga aactatcata taatcataag tttgagccta gaagtgatcc ttgtgatctt
     5341 ctcacctctt taaattccca caacacaaga gattaaaaac agaggtttca gctcttcata
     5401 gtgcgttgtg aaatggctgg ccagagtgta ccaacaaagc tgtcatcggg ctcacagctc
     5461 agagacatct gcatgtgatc atctgcatag tcctctcctc taacgggaaa cacctcagat
     5521 ttgcatataa aaaagcaccc tggtgctgaa atgaacccct ttcttgaaca tcaaagctgt
     5581 ctcccacagc cttgggcagc agggtgcctc ttagtggatg tgctgggtcc accctgagcc
     5641 ctgacatgtg gtggcagcat tgccagttgg tctgtgtgtc tgtgtagcag ggacgatttc
     5701 ccagaaagca attttccttt tgaaatacgt aattgttgag actaggcagt ttcaaagtca
     5761 gctgcatata gtagcaagta caggactgtc ttgtttttgg tgtccttgga ggtgctgggg
     5821 tgagggtttc agtgggatca tttactctca catgttgtct gccttctgct tctgtggaca
     5881 ctgctttgta cttaattcag acagactgtg aatacacctt ttttataaat acctttcaaa
     5941 ttcttggtaa gatataattt tgatagctga ttgcagattt tctgtatttg tcagattaat
     6001 aaagactgca tgaatcca
//
