LOCUS       NM_002773               1837 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens serine protease 8 (PRSS8), mRNA.
ACCESSION   NM_002773
VERSION     NM_002773.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Huang,N., Wang,Q., Bernard,R.B., Chen,C.Y., Hu,J.M., Wang,J.K.,
            Chan,K.S., Johnson,M.D. and Lin,C.Y.
  TITLE     SPINT2 mutations in the Kunitz domain 2 found in SCSD patients
            inactivate HAI-2 as prostasin inhibitor via abnormal protein
            folding and N-glycosylation
  JOURNAL   Hum Mol Genet 33 (9), 752-767 (2024)
   PUBMED   38271183
  REMARK    GeneRIF: SPINT2 mutations in the Kunitz domain 2 found in SCSD
            patients inactivate HAI-2 as prostasin inhibitor via abnormal
            protein folding and N-glycosylation.
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Zhao,X., Lv,S., Li,N., Zou,Q., Sun,L. and Song,T.
  TITLE     YTHDF2 protein stabilization by the deubiquitinase OTUB1 promotes
            prostate cancer cell proliferation via PRSS8 mRNA degradation
  JOURNAL   J Biol Chem 300 (4), 107152 (2024)
   PUBMED   38462165
  REMARK    GeneRIF: YTHDF2 protein stabilization by the deubiquitinase OTUB1
            promotes prostate cancer cell proliferation via PRSS8 mRNA
            degradation.
REFERENCE   3  (bases 1 to 1837)
  AUTHORS   Shamseldin,H.E., Derar,N., Alzaidan,H., AlHathal,N., Alfalah,A.,
            Abdulwahab,F., Alzaid,T., Alkeraye,S., Alobaida,S.A. and
            Alkuraya,F.S.
  TITLE     PRSS8, encoding prostasin, is mutated in patients with autosomal
            recessive ichthyosis
  JOURNAL   Hum Genet 142 (4), 477-482 (2023)
   PUBMED   36715754
  REMARK    GeneRIF: PRSS8, encoding prostasin, is mutated in patients with
            autosomal recessive ichthyosis.
REFERENCE   4  (bases 1 to 1837)
  AUTHORS   Cai,C., Zhang,Y. and Peng,X.
  TITLE     Knocking down Sterol regulatory element binding protein 2 (SREBF2)
            inhibits the Serine Protease 8 (PRSS8) /sodium channel epithelial
            1alpha subunit (SCNN1A) axis to reduce the cell proliferation,
            migration and epithelial-mesenchymal transformation of ovarian
            cancer
  JOURNAL   Bioengineered 12 (2), 9390-9400 (2021)
   PUBMED   34823420
  REMARK    GeneRIF: Knocking down Sterol regulatory element binding protein 2
            (SREBF2) inhibits the Serine Protease 8 (PRSS8) /sodium channel
            epithelial 1alpha subunit (SCNN1A) axis to reduce the cell
            proliferation, migration and epithelial-mesenchymal transformation
            of ovarian cancer.
REFERENCE   5  (bases 1 to 1837)
  AUTHORS   Aggarwal,S., Dabla,P.K. and Arora,S.
  TITLE     Prostasin: An Epithelial Sodium Channel Regulator
  JOURNAL   J Biomark 2013, 179864 (2013)
   PUBMED   26317012
  REMARK    Review article
REFERENCE   6  (bases 1 to 1837)
  AUTHORS   Chen,L.M., Skinner,M.L., Kauffman,S.W., Chao,J., Chao,L.,
            Thaler,C.D. and Chai,K.X.
  TITLE     Prostasin is a glycosylphosphatidylinositol-anchored active serine
            protease
  JOURNAL   J Biol Chem 276 (24), 21434-21442 (2001)
   PUBMED   11274175
REFERENCE   7  (bases 1 to 1837)
  AUTHORS   Nelson,P.S., Gan,L., Ferguson,C., Moss,P., Gelinas,R., Hood,L. and
            Wang,K.
  TITLE     Molecular cloning and characterization of prostase, an
            androgen-regulated serine protease with prostate-restricted
            expression
  JOURNAL   Proc Natl Acad Sci U S A 96 (6), 3114-3119 (1999)
   PUBMED   10077646
REFERENCE   8  (bases 1 to 1837)
  AUTHORS   Yu,J.X., Chao,L., Ward,D.C. and Chao,J.
  TITLE     Structure and chromosomal localization of the human prostasin
            (PRSS8) gene
  JOURNAL   Genomics 32 (3), 334-340 (1996)
   PUBMED   8838796
REFERENCE   9  (bases 1 to 1837)
  AUTHORS   Yu,J.X., Chao,L. and Chao,J.
  TITLE     Molecular cloning, tissue-specific expression, and cellular
            localization of human prostasin mRNA
  JOURNAL   J Biol Chem 270 (22), 13483-13489 (1995)
   PUBMED   7768952
REFERENCE   10 (bases 1 to 1837)
  AUTHORS   Yu,J.X., Chao,L. and Chao,J.
  TITLE     Prostasin is a novel human serine proteinase from seminal fluid.
            Purification, tissue distribution, and localization in prostate
            gland
  JOURNAL   J Biol Chem 269 (29), 18843-18848 (1994)
   PUBMED   8034638
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from L41351.1 and BC001462.2.
            
            On Nov 22, 2018 this sequence version replaced NM_002773.4.
            
            Summary: This gene encodes a member of the peptidase S1 or
            chymotrypsin family of serine proteases. The encoded preproprotein
            is proteolytically processed to generate light and heavy chains
            that associate via a disulfide bond to form the heterodimeric
            enzyme. This enzyme is highly expressed in prostate epithelia and
            is one of several proteolytic enzymes found in seminal fluid. This
            protease exhibits trypsin-like substrate specificity, cleaving
            protein substrates at the carboxyl terminus of lysine or arginine
            residues. The encoded protease partially mediates proteolytic
            activation of the epithelial sodium channel, a regulator of sodium
            balance, and may also play a role in epithelial barrier formation.
            [provided by RefSeq, Feb 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.525605.1,
                                           ERR279853.1190.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317508.11/ ENSP00000319730.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               L41351.1           1-226
            227-1837            BC001462.2         173-1783
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p11.2"
     gene            1..1837
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /note="serine protease 8"
                     /db_xref="GeneID:5652"
                     /db_xref="HGNC:HGNC:9491"
                     /db_xref="MIM:600823"
     exon            1..314
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..1261
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /note="channel-activating protease 1; protease, serine 8;
                     channel-activating serine protease 1"
                     /codon_start=1
                     /product="prostasin preproprotein"
                     /protein_id="NP_002764.1"
                     /db_xref="CCDS:CCDS45469.1"
                     /db_xref="GeneID:5652"
                     /db_xref="HGNC:HGNC:9491"
                     /db_xref="MIM:600823"
                     /translation="MAQKGVLGPGQLGAVAILLYLGLLRSGTGAEGAEAPCGVAPQAR
                     ITGGSSAVAGQWPWQVSITYEGVHVCGGSLVSEQWVLSAAHCFPSEHHKEAYEVKLGA
                     HQLDSYSEDAKVSTLKDIIPHPSYLQEGSQGDIALLQLSRPITFSRYIRPICLPAANA
                     SFPNGLHCTVTGWGHVAPSVSLLTPKPLQQLEVPLISRETCNCLYNIDAKPEEPHFVQ
                     EDMVCAGYVEGGKDACQGDSGGPLSCPVEGLWYLTGIVSWGDACGARNRPGVYTLASS
                     YASWIQSKVTELQPRVVPQTQESQPDSNLCGSHLAFSSAPAQGLLRPILFLPLGLALG
                     LLSPWLSEH"
     exon            315..332
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /inference="alignment:Splign:2.1.0"
     exon            333..495
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /inference="alignment:Splign:2.1.0"
     exon            496..767
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /inference="alignment:Splign:2.1.0"
     exon            768..934
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1837
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1818..1823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /note="hexamer: ATTAAA"
     polyA_site      1837
                     /gene="PRSS8"
                     /gene_synonym="CAP1; PROSTASIN"
                     /note="major polyA site"
ORIGIN      
        1 agacggtgct ggtgactcgt ccacactgct cgcttcggat actccaggcg tctcccgttg
       61 cggccgctcc ctgccttaga ggccagcctt ggacacttgc tgcccctttc cagcccggat
      121 tctgggatcc ttccctctga gccaacatct gggtcctgcc ttcgacacca ccccaaggct
      181 tcctaccttg cgtgcctgga gtctgcccca ggggcccttg tcctgggcca tggcccagaa
      241 gggggtcctg gggcctgggc agctgggggc tgtggccatt ctgctctatc ttggattact
      301 ccggtcgggg acaggagcgg aaggggcaga agctccctgc ggtgtggccc cccaagcacg
      361 catcacaggt ggcagcagtg cagtcgccgg tcagtggccc tggcaggtca gcatcaccta
      421 tgaaggcgtc catgtgtgtg gtggctctct cgtgtctgag cagtgggtgc tgtcagctgc
      481 tcactgcttc cccagcgagc accacaagga agcctatgag gtcaagctgg gggcccacca
      541 gctagactcc tactccgagg acgccaaggt cagcaccctg aaggacatca tcccccaccc
      601 cagctacctc caggagggct cccagggcga cattgcactc ctccaactca gcagacccat
      661 caccttctcc cgctacatcc ggcccatctg cctccctgca gccaacgcct ccttccccaa
      721 cggcctccac tgcactgtca ctggctgggg tcatgtggcc ccctcagtga gcctcctgac
      781 gcccaagcca ctgcagcaac tcgaggtgcc tctgatcagt cgtgagacgt gtaactgcct
      841 gtacaacatc gacgccaagc ctgaggagcc gcactttgtc caagaggaca tggtgtgtgc
      901 tggctatgtg gaggggggca aggacgcctg ccagggtgac tctgggggcc cactctcctg
      961 ccctgtggag ggtctctggt acctgacggg cattgtgagc tggggagatg cctgtggggc
     1021 ccgcaacagg cctggtgtgt acactctggc ctccagctat gcctcctgga tccaaagcaa
     1081 ggtgacagaa ctccagcctc gtgtggtgcc ccaaacccag gagtcccagc ccgacagcaa
     1141 cctctgtggc agccacctgg ccttcagctc tgccccagcc cagggcttgc tgaggcccat
     1201 ccttttcctg cctctgggcc tggctctggg cctcctctcc ccatggctca gcgagcactg
     1261 agctggccct acttccagga tggatgcatc acactcaagg acaggagcct ggtccttccc
     1321 tgatggcctt tggacccagg gcctgacttg agccactcct tccttcagga ctctgcggga
     1381 ggctggggcc ccatcttgat ctttgagccc attcttctgg gtgtgctttt tgggaccatc
     1441 actgagagtc aggagtttta ctgcctgtag caatggccag agcctctggc ccctcaccca
     1501 ccatggacca gcccattggc cgagctcctg gggagctcct gggacccttg gctatgaaaa
     1561 tgagccctgg ctcccacctg tttctggaag actgctcccg gcccgcctgc ccagactgat
     1621 gagcacatct ctctgccctc tccctgtgtt ctgggctggg gccacctttg tgcagcttcg
     1681 aggacaggaa aggccccaat cttgcccact ggccgctgag cgcccccgag ccctgactcc
     1741 tggactccgg aggactgagc ccccaccgga actgggctgg cgcttggatc tggggtggga
     1801 gtaacagggc agaaatgatt aaaatgtttg agcacaa
//
