LOCUS       NM_214710               1044 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens serine protease 57 (PRSS57), transcript variant 1,
            mRNA.
ACCESSION   NM_214710
VERSION     NM_214710.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Lin,X., Sha,Z., Trimpert,J., Kunec,D., Jiang,C., Xiong,Y., Xu,B.,
            Zhu,Z., Xue,W. and Wu,H.
  TITLE     The NSP4 T492I mutation increases SARS-CoV-2 infectivity by
            altering non-structural protein cleavage
  JOURNAL   Cell Host Microbe 31 (7), 1170-1184 (2023)
   PUBMED   37402373
  REMARK    GeneRIF: The NSP4 T492I mutation increases SARS-CoV-2 infectivity
            by altering non-structural protein cleavage.
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Wysocka,M., Gruba,N., Grzywa,R., Gieldon,A., Bachor,R.,
            Brzozowski,K., Sienczyk,M., Dieter,J., Szewczuk,Z., Rolka,K. and
            Lesner,A.
  TITLE     PEGylated substrates of NSP4 protease: A tool to study protease
            specificity
  JOURNAL   Sci Rep 6, 22856 (2016)
   PUBMED   26955973
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1044)
  AUTHORS   Kasperkiewicz,P., Poreba,M., Snipas,S.J., Lin,S.J., Kirchhofer,D.,
            Salvesen,G.S. and Drag,M.
  TITLE     Design of a Selective Substrate and Activity Based Probe for Human
            Neutrophil Serine Protease 4
  JOURNAL   PLoS One 10 (7), e0132818 (2015)
   PUBMED   26172376
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1044)
  AUTHORS   Lin,S.J., Dong,K.C., Eigenbrot,C., van Lookeren Campagne,M. and
            Kirchhofer,D.
  TITLE     Structures of neutrophil serine protease 4 reveal an unusual
            mechanism of substrate recognition by a trypsin-fold protease
  JOURNAL   Structure 22 (9), 1333-1340 (2014)
   PUBMED   25156428
  REMARK    GeneRIF: Structural insights in to substrate recognition by the
            trypsin-fold protease NSP4.
REFERENCE   5  (bases 1 to 1044)
  AUTHORS   Perera,N.C., Wiesmuller,K.H., Larsen,M.T., Schacher,B.,
            Eickholz,P., Borregaard,N. and Jenne,D.E.
  TITLE     NSP4 is stored in azurophil granules and released by activated
            neutrophils as active endoprotease with restricted specificity
  JOURNAL   J Immunol 191 (5), 2700-2707 (2013)
   PUBMED   23904161
  REMARK    GeneRIF: cathepsin C was identified as the activator of NSP4 in
            vivo, as cathepsin C deficiency resulted in a complete absence of
            NSP4 in a Papillon-Lefevre patient
REFERENCE   6  (bases 1 to 1044)
  AUTHORS   Perera,N.C., Schilling,O., Kittel,H., Back,W., Kremmer,E. and
            Jenne,D.E.
  TITLE     NSP4, an elastase-related protease in human neutrophils with
            arginine specificity
  JOURNAL   Proc Natl Acad Sci U S A 109 (16), 6229-6234 (2012)
   PUBMED   22474388
REFERENCE   7  (bases 1 to 1044)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   8  (bases 1 to 1044)
  AUTHORS   Polikoff,D., Kuo,W.L., Cochran,J.F., Wernick,M., Kowbel,D.,
            Myambo,K. and Collins,C.C.
  TITLE     Assignment of protease, serine-like 1 (PRSSL1) to human chromosome
            19q13 by in situ hybridization and radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 79 (1-2), 147-148 (1997)
   PUBMED   9533035
REFERENCE   9  (bases 1 to 1044)
  AUTHORS   Okubo,K., Itoh,K., Fukushima,A., Yoshii,J. and Matsubara,K.
  TITLE     Monitoring cell physiology by expression profiles and discovering
            cell type-specific genes by compiled expression profiles
  JOURNAL   Genomics 30 (2), 178-186 (1995)
   PUBMED   8586417
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC112708.3 and AC004156.1.
            
            On Jun 2, 2019 this sequence version replaced NM_214710.4.
            
            Summary: This gene encodes an arginine-specific serine protease and
            member of the peptidase S1 family of proteins. The encoded protein
            may undergo proteolytic activation before storage in azurophil
            granules, in neutrophil cells of the immune system. Following
            neutrophil activation, the protease is released into the
            pericellular environment, where it may play a role in defense
            against microbial pathogens. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358594.2, BC137161.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2163105 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AC112708.3         14389-14489         c
            102-258             AC112708.3         13851-14007         c
            259-403             AC112708.3         10895-11039         c
            404-667             AC112708.3         5962-6225           c
            668-1044            AC004156.1         40963-41339         c
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1044
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /note="serine protease 57"
                     /db_xref="GeneID:400668"
                     /db_xref="HGNC:HGNC:31397"
     exon            1..101
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..874
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; serine protease 1-like protein 1; neutrophil
                     serine protease 4; protease, serine 57"
                     /codon_start=1
                     /product="serine protease 57 isoform 1 precursor"
                     /protein_id="NP_999875.2"
                     /db_xref="CCDS:CCDS12041.1"
                     /db_xref="GeneID:400668"
                     /db_xref="HGNC:HGNC:31397"
                     /translation="MGLGLRGWGRPLLTVATALMLPVKPPAGSWGAQIIGGHEVTPHS
                     RPYMASVRFGGQHHCGGFLLRARWVVSAAHCFSHRDLRTGLVVLGAHVLSTAEPTQQV
                     FGIDALTTHPDYHPMTHANDICLLRLNGSAVLGPAVGLLRPPGRRARPPTAGTRCRVA
                     GWGFVSDFEELPPGLMEAKVRVLDPDVCNSSWKGHLTLTMLCTRSGDSHRRGFCSADS
                     GGPLVCRNRAHGLVSFSGLWCGDPKTPDVYTQVSAFVAWIWDVVRRSSPQPGPLPGTT
                     RPPGEAA"
     exon            102..258
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /inference="alignment:Splign:2.1.0"
     exon            259..403
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /inference="alignment:Splign:2.1.0"
     exon            404..667
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /inference="alignment:Splign:2.1.0"
     exon            668..1044
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1027..1032
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /note="hexamer: AGTAAA"
     polyA_site      1044
                     /gene="PRSS57"
                     /gene_synonym="NSP4; PRSSL1; UNQ782"
                     /note="major polyA site"
ORIGIN      
        1 gagacgccag cctgcgtctg ccatggggct cgggttgagg ggctggggac gtcctctgct
       61 gactgtggcc accgccctga tgctgcccgt gaagcccccc gcaggctcct ggggggccca
      121 gatcatcggg ggccacgagg tgacccccca ctccaggccc tacatggcat ccgtgcgctt
      181 cgggggccaa catcactgcg gaggcttcct gctgcgagcc cgctgggtgg tctcggccgc
      241 ccactgcttc agccacagag acctccgcac tggcctggtg gtgctgggcg cccacgtcct
      301 gagtactgcg gagcccaccc agcaggtgtt tggcatcgat gctctcacca cacaccccga
      361 ctaccacccc atgacccacg ccaacgacat ctgcctgctg cggctgaacg gctctgctgt
      421 cctgggccct gcagtggggc tgctgaggcc gccagggaga agggccaggc cccccacagc
      481 ggggacacgg tgccgggtgg ctggctgggg cttcgtgtct gactttgagg agctgccgcc
      541 tggactgatg gaggccaagg tccgagtgct ggacccggac gtctgcaaca gctcctggaa
      601 gggccacctg acacttacca tgctctgcac ccgcagtggg gacagccaca gacggggctt
      661 ctgctcggcc gactccggag ggcccctggt gtgcaggaac cgggctcacg gcctcgtttc
      721 cttctcgggc ctctggtgcg gcgaccccaa gacccccgac gtgtacacgc aggtgtccgc
      781 ctttgtggcc tggatctggg acgtggttcg gcggagcagt ccccagcccg gccccctgcc
      841 tgggaccacc aggcccccag gagaagccgc ctgagccaca accttgcggc atgcaaatga
      901 gatggccgct ccaggcctgg aatgttccgt ggctgggccc cacgggaagc ctgatgttca
      961 gggttggggt gggacgggca gcggtggggc acacccattc cacatgcaaa gggcagaagc
     1021 aaacccagta aaatgttaac tgac
//
