LOCUS       NM_013270               1332 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens serine protease 50 (PRSS50), mRNA.
ACCESSION   NM_013270
VERSION     NM_013270.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Youssef,H.M.K., Radi,D.A. and Abd El-Azeem,M.A.
  TITLE     Expression of TSP50, SERCA2 and IL-8 in Colorectal Adenoma and
            Carcinoma: Correlation to Clinicopathological Factors
  JOURNAL   Pathol Oncol Res 27, 1609990 (2021)
   PUBMED   34744521
  REMARK    GeneRIF: Expression of TSP50, SERCA2 and IL-8 in Colorectal Adenoma
            and Carcinoma: Correlation to Clinicopathological Factors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Wang,Y., Xu,S., Wang,S., Song,Z., Zheng,L., Wang,G., Sun,Y. and
            Bao,Y.
  TITLE     miR-4709-3p Inhibits Cell Proliferation by Downregulating TSP50
            Expression in Breast Cancer Cells
  JOURNAL   DNA Cell Biol 40 (7), 969-978 (2021)
   PUBMED   33956530
  REMARK    GeneRIF: miR-4709-3p Inhibits Cell Proliferation by Downregulating
            TSP50 Expression in Breast Cancer Cells.
REFERENCE   3  (bases 1 to 1332)
  AUTHORS   Gao,F., Zhang,X., Wang,S., Zheng,L., Sun,Y., Wang,G., Song,Z. and
            Bao,Y.
  TITLE     TSP50 promotes the Warburg effect and hepatocyte proliferation via
            regulating PKM2 acetylation
  JOURNAL   Cell Death Dis 12 (6), 517 (2021)
   PUBMED   34016961
  REMARK    GeneRIF: TSP50 promotes the Warburg effect and hepatocyte
            proliferation via regulating PKM2 acetylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1332)
  AUTHORS   Zhang,X., Gao,F., Ai,H., Wang,S., Song,Z., Zheng,L., Wang,G.,
            Sun,Y. and Bao,Y.
  TITLE     TSP50 promotes hepatocyte proliferation and tumour formation by
            activating glucose-6-phosphate dehydrogenase (G6PD)
  JOURNAL   Cell Prolif 54 (4), e13015 (2021)
   PUBMED   33630390
  REMARK    GeneRIF: TSP50 promotes hepatocyte proliferation and tumour
            formation by activating glucose-6-phosphate dehydrogenase (G6PD).
REFERENCE   5  (bases 1 to 1332)
  AUTHORS   Cao,Q.H., Liu,F., Li,C.Z., Liu,N., Shu,M., Lin,Y., Ding,L. and
            Xue,L.
  TITLE     Testes-specific protease 50 (TSP50) promotes invasion and
            metastasis by inducing EMT in gastric cancer
  JOURNAL   BMC Cancer 18 (1), 94 (2018)
   PUBMED   29361914
  REMARK    GeneRIF: TSP50 promotes the proliferation, migration and invasion
            of gastric cancer cells involving NF-kappaB dependent EMT
            activation. Targeting TSP50 may provide a novel therapeutic
            strategy for the management of gastric cancer
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1332)
  AUTHORS   Wang,M., Bao,Y.L., Wu,Y., Yu,C.L., Meng,X., Xu,H.P. and Li,Y.X.
  TITLE     Identification and characterization of the human testes-specific
            protease 50 gene promoter
  JOURNAL   DNA Cell Biol 27 (6), 307-314 (2008)
   PUBMED   18462069
  REMARK    GeneRIF: Overexpression of Sp1 and C/EBPbeta transcriptional
            factors upregulated the activities of the TSP50 promoter.
REFERENCE   7  (bases 1 to 1332)
  AUTHORS   Xu,H., Shan,J., Jurukovski,V., Yuan,L., Li,J. and Tian,K.
  TITLE     TSP50 encodes a testis-specific protease and is negatively
            regulated by p53
  JOURNAL   Cancer Res 67 (3), 1239-1245 (2007)
   PUBMED   17283160
REFERENCE   8  (bases 1 to 1332)
  AUTHORS   Xu,H.P., Yuan,L., Shan,J. and Feng,H.
  TITLE     Localization and expression of TSP50 protein in human and rodent
            testes
  JOURNAL   Urology 64 (4), 826-832 (2004)
   PUBMED   15491742
  REMARK    GeneRIF: TSP50 was largely downregulated in all testicular germ
            cell tumors. It may function in mammalian spermatogenesis. Its
            linear catalytic structure is similar to many serine proteases,
            except for a Thr-for-Ser residual catalytic site substitution.
REFERENCE   9  (bases 1 to 1332)
  AUTHORS   Shan,J., Yuan,L., Xiao,Q., Chiorazzi,N., Budman,D., Teichberg,S.
            and Xu,H.P.
  TITLE     TSP50, a possible protease in human testes, is activated in breast
            cancer epithelial cells
  JOURNAL   Cancer Res 62 (1), 290-294 (2002)
   PUBMED   11782390
  REMARK    GeneRIF: TSP50, a possible protease in human testes, is activated
            in breast cancer epithelial cells.
REFERENCE   10 (bases 1 to 1332)
  AUTHORS   Yuan,L., Shan,J., De Risi,D., Broome,J., Lovecchio,J., Gal,D.,
            Vinciguerra,V. and Xu,H.P.
  TITLE     Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment
            in human breast cancer
  JOURNAL   Cancer Res 59 (13), 3215-3221 (1999)
   PUBMED   10397268
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF100707.1, BC037775.2 and BC033016.1.
            
            On Nov 23, 2018 this sequence version replaced NM_013270.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC033016.1, AF100707.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148093, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315170.13/ ENSP00000326598.7
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1226              AF100707.1         15-1240
            1227-1330           BC037775.2         1203-1306
            1331-1332           BC033016.1         1336-1337
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..1332
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /note="serine protease 50"
                     /db_xref="GeneID:29122"
                     /db_xref="HGNC:HGNC:17910"
                     /db_xref="MIM:607950"
     exon            1..151
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..1203
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /note="testes-specific protease 50; cancer/testis antigen
                     20; testis-specific protease-like protein 50; probable
                     threonine protease PRSS50; testicular tissue protein Li
                     210; protease, serine 50"
                     /codon_start=1
                     /product="probable threonine protease PRSS50 precursor"
                     /protein_id="NP_037402.1"
                     /db_xref="CCDS:CCDS2745.1"
                     /db_xref="GeneID:29122"
                     /db_xref="HGNC:HGNC:17910"
                     /db_xref="MIM:607950"
                     /translation="MGRWCQTVARGQRPRTSAPSRAGALLLLLLLLRSAGCWGAGEAP
                     GALSTADPADQSVQCVPKATCPSSRPRLLWQTPTTQTLPSTTMETQFPVSEGKVDPYR
                     SCGFSYEQDPTLRDPEAVARRWPWMVSVRANGTHICAGTIIASQWVLTVAHCLIWRDV
                     IYSVRVGSPWIDQMTQTASDVPVLQVIMHSRYRAQRFWSWVGQANDIGLLKLKQELKY
                     SNYVRPICLPGTDYVLKDHSRCTVTGWGLSKADGMWPQFRTIQEKEVIILNNKECDNF
                     YHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYLVGLVSWGAGC
                     QKSEAPPIYLQVSSYQHWIWDCLNGQALALPAPSRTLLLALPLPLSLLAAL"
     exon            152..352
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /inference="alignment:Splign:2.1.0"
     exon            353..515
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /inference="alignment:Splign:2.1.0"
     exon            516..799
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /inference="alignment:Splign:2.1.0"
     exon            800..966
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1332
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1310..1315
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /note="hexamer: ATTAAA"
     polyA_site      1332
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /note="major polyA site"
ORIGIN      
        1 actgggagcg ccttccggag agacgcagtc ggctgccacc ccgggatggg tcgctggtgc
       61 cagaccgtcg cgcgcgggca gcgcccccgg acgtctgccc cctcccgcgc cggtgccctg
      121 ctgctgctgc ttctgttgct gaggtctgca ggttgctggg gcgcagggga agccccgggg
      181 gcgctgtcca ctgctgatcc cgccgaccag agcgtccagt gtgtccccaa ggccacctgt
      241 ccttccagcc ggcctcgcct tctctggcag accccgacca cccagacact gccctcgacc
      301 accatggaga cccaattccc agtttctgaa ggcaaagtcg acccataccg ctcctgtggc
      361 ttttcctacg agcaggaccc caccctcagg gacccagaag ccgtggctcg gcggtggccc
      421 tggatggtca gcgtgcgggc caatggcaca cacatctgtg ccggcaccat cattgcctcc
      481 cagtgggtgc tgactgtggc ccactgcctg atctggcgtg atgttatcta ctcagtgagg
      541 gtggggagtc cgtggattga ccagatgacg cagaccgcct ccgatgtccc ggtgctccag
      601 gtcatcatgc atagcaggta ccgggcccag cggttctggt cctgggtggg ccaggccaac
      661 gacatcggcc tcctcaagct caagcaggaa ctcaagtaca gcaattacgt gcggcccatc
      721 tgcctgcctg gcacggacta tgtgttgaag gaccattccc gctgcactgt gacgggctgg
      781 ggactttcca aggctgacgg catgtggcct cagttccgga ccattcagga gaaggaagtc
      841 atcatcctga acaacaaaga gtgtgacaat ttctaccaca acttcaccaa aatccccact
      901 ctggttcaga tcatcaagtc ccagatgatg tgtgcggagg acacccacag ggagaagttc
      961 tgctatgagc taactggaga gcccttggtc tgctccatgg agggcacgtg gtacctggtg
     1021 ggattggtga gctggggtgc aggctgccag aagagcgagg ccccacccat ctacctacag
     1081 gtctcctcct accaacactg gatctgggac tgcctcaacg ggcaggccct ggccctgcca
     1141 gccccatcca ggaccctgct cctggcactc ccactgcccc tcagcctcct tgctgccctc
     1201 tgactctgtg tgccctccct cacttgtggg ccccccttgc ctccgtgccc aggttgctgt
     1261 gggtgcagct gtcacagccc tgagagtcag ggtggagatg aggtgctcaa ttaaacatta
     1321 ctgttttcca tg
//
