LOCUS       NP_037402                385 aa            linear   PRI 04-APR-2024
DEFINITION  probable threonine protease PRSS50 precursor [Homo sapiens].
ACCESSION   NP_037402
VERSION     NP_037402.1
DBSOURCE    REFSEQ: accession NM_013270.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 385)
  AUTHORS   Youssef,H.M.K., Radi,D.A. and Abd El-Azeem,M.A.
  TITLE     Expression of TSP50, SERCA2 and IL-8 in Colorectal Adenoma and
            Carcinoma: Correlation to Clinicopathological Factors
  JOURNAL   Pathol Oncol Res 27, 1609990 (2021)
   PUBMED   34744521
  REMARK    GeneRIF: Expression of TSP50, SERCA2 and IL-8 in Colorectal Adenoma
            and Carcinoma: Correlation to Clinicopathological Factors.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 385)
  AUTHORS   Wang,Y., Xu,S., Wang,S., Song,Z., Zheng,L., Wang,G., Sun,Y. and
            Bao,Y.
  TITLE     miR-4709-3p Inhibits Cell Proliferation by Downregulating TSP50
            Expression in Breast Cancer Cells
  JOURNAL   DNA Cell Biol 40 (7), 969-978 (2021)
   PUBMED   33956530
  REMARK    GeneRIF: miR-4709-3p Inhibits Cell Proliferation by Downregulating
            TSP50 Expression in Breast Cancer Cells.
REFERENCE   3  (residues 1 to 385)
  AUTHORS   Gao,F., Zhang,X., Wang,S., Zheng,L., Sun,Y., Wang,G., Song,Z. and
            Bao,Y.
  TITLE     TSP50 promotes the Warburg effect and hepatocyte proliferation via
            regulating PKM2 acetylation
  JOURNAL   Cell Death Dis 12 (6), 517 (2021)
   PUBMED   34016961
  REMARK    GeneRIF: TSP50 promotes the Warburg effect and hepatocyte
            proliferation via regulating PKM2 acetylation.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 385)
  AUTHORS   Zhang,X., Gao,F., Ai,H., Wang,S., Song,Z., Zheng,L., Wang,G.,
            Sun,Y. and Bao,Y.
  TITLE     TSP50 promotes hepatocyte proliferation and tumour formation by
            activating glucose-6-phosphate dehydrogenase (G6PD)
  JOURNAL   Cell Prolif 54 (4), e13015 (2021)
   PUBMED   33630390
  REMARK    GeneRIF: TSP50 promotes hepatocyte proliferation and tumour
            formation by activating glucose-6-phosphate dehydrogenase (G6PD).
REFERENCE   5  (residues 1 to 385)
  AUTHORS   Cao,Q.H., Liu,F., Li,C.Z., Liu,N., Shu,M., Lin,Y., Ding,L. and
            Xue,L.
  TITLE     Testes-specific protease 50 (TSP50) promotes invasion and
            metastasis by inducing EMT in gastric cancer
  JOURNAL   BMC Cancer 18 (1), 94 (2018)
   PUBMED   29361914
  REMARK    GeneRIF: TSP50 promotes the proliferation, migration and invasion
            of gastric cancer cells involving NF-kappaB dependent EMT
            activation. Targeting TSP50 may provide a novel therapeutic
            strategy for the management of gastric cancer
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 385)
  AUTHORS   Wang,M., Bao,Y.L., Wu,Y., Yu,C.L., Meng,X., Xu,H.P. and Li,Y.X.
  TITLE     Identification and characterization of the human testes-specific
            protease 50 gene promoter
  JOURNAL   DNA Cell Biol 27 (6), 307-314 (2008)
   PUBMED   18462069
  REMARK    GeneRIF: Overexpression of Sp1 and C/EBPbeta transcriptional
            factors upregulated the activities of the TSP50 promoter.
REFERENCE   7  (residues 1 to 385)
  AUTHORS   Xu,H., Shan,J., Jurukovski,V., Yuan,L., Li,J. and Tian,K.
  TITLE     TSP50 encodes a testis-specific protease and is negatively
            regulated by p53
  JOURNAL   Cancer Res 67 (3), 1239-1245 (2007)
   PUBMED   17283160
REFERENCE   8  (residues 1 to 385)
  AUTHORS   Xu,H.P., Yuan,L., Shan,J. and Feng,H.
  TITLE     Localization and expression of TSP50 protein in human and rodent
            testes
  JOURNAL   Urology 64 (4), 826-832 (2004)
   PUBMED   15491742
  REMARK    GeneRIF: TSP50 was largely downregulated in all testicular germ
            cell tumors. It may function in mammalian spermatogenesis. Its
            linear catalytic structure is similar to many serine proteases,
            except for a Thr-for-Ser residual catalytic site substitution.
REFERENCE   9  (residues 1 to 385)
  AUTHORS   Shan,J., Yuan,L., Xiao,Q., Chiorazzi,N., Budman,D., Teichberg,S.
            and Xu,H.P.
  TITLE     TSP50, a possible protease in human testes, is activated in breast
            cancer epithelial cells
  JOURNAL   Cancer Res 62 (1), 290-294 (2002)
   PUBMED   11782390
  REMARK    GeneRIF: TSP50, a possible protease in human testes, is activated
            in breast cancer epithelial cells.
REFERENCE   10 (residues 1 to 385)
  AUTHORS   Yuan,L., Shan,J., De Risi,D., Broome,J., Lovecchio,J., Gal,D.,
            Vinciguerra,V. and Xu,H.P.
  TITLE     Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment
            in human breast cancer
  JOURNAL   Cancer Res 59 (13), 3215-3221 (1999)
   PUBMED   10397268
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF100707.1, BC037775.2 and BC033016.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC033016.1, AF100707.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148093, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315170.13/ ENSP00000326598.7
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     Protein         1..385
                     /product="probable threonine protease PRSS50 precursor"
                     /note="testes-specific protease 50; cancer/testis antigen
                     20; testis-specific protease-like protein 50; probable
                     threonine protease PRSS50; testicular tissue protein Li
                     210; protease, serine 50"
                     /calculated_mol_wt=38882
     sig_peptide     1..39
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=4224
     mat_peptide     40..385
                     /product="Probable threonine protease PRSS50.
                     /id=PRO_0000028492"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UI38.1)"
                     /calculated_mol_wt=38882
     Region          118..353
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            133
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UI38.1)"
     Site            order(153,206,310)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            279
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UI38.1)"
     CDS             1..385
                     /gene="PRSS50"
                     /gene_synonym="CT20; TSP50"
                     /coded_by="NM_013270.5:46..1203"
                     /db_xref="CCDS:CCDS2745.1"
                     /db_xref="GeneID:29122"
                     /db_xref="HGNC:HGNC:17910"
                     /db_xref="MIM:607950"
ORIGIN      
        1 mgrwcqtvar gqrprtsaps ragallllll llrsagcwga geapgalsta dpadqsvqcv
       61 pkatcpssrp rllwqtpttq tlpsttmetq fpvsegkvdp yrscgfsyeq dptlrdpeav
      121 arrwpwmvsv rangthicag tiiasqwvlt vahcliwrdv iysvrvgspw idqmtqtasd
      181 vpvlqvimhs ryraqrfwsw vgqandigll klkqelkysn yvrpiclpgt dyvlkdhsrc
      241 tvtgwglska dgmwpqfrti qekeviilnn kecdnfyhnf tkiptlvqii ksqmmcaedt
      301 hrekfcyelt geplvcsmeg twylvglvsw gagcqkseap piylqvssyq hwiwdclngq
      361 alalpapsrt lllalplpls llaal
//
