LOCUS       NM_001197097             932 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens serine protease 3 (PRSS3), transcript variant 3, mRNA.
ACCESSION   NM_001197097
VERSION     NM_001197097.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Zhou,M., Li,K. and Luo,K.Q.
  TITLE     Shear Stress Drives the Cleavage Activation of Protease-Activated
            Receptor 2 by PRSS3/Mesotrypsin to Promote Invasion and Metastasis
            of Circulating Lung Cancer Cells
  JOURNAL   Adv Sci (Weinh) 10 (25), e2301059 (2023)
   PUBMED   37395651
  REMARK    GeneRIF: Shear Stress Drives the Cleavage Activation of
            Protease-Activated Receptor 2 by PRSS3/Mesotrypsin to Promote
            Invasion and Metastasis of Circulating Lung Cancer Cells.
REFERENCE   2  (bases 1 to 932)
  AUTHORS   Zheng,L., Wang,X., Zheng,W. and Huang,H.
  TITLE     Serine Protease 3 Promotes Progression of Diffuse Large B-Cell
            Lymphoma and Serves as a Novel Prognostic Predictor
  JOURNAL   Dis Markers 2022, 1254790 (2022)
   PUBMED   36618965
  REMARK    GeneRIF: Serine Protease 3 Promotes Progression of Diffuse Large
            B-Cell Lymphoma and Serves as a Novel Prognostic Predictor.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 932)
  AUTHORS   Rattanakomol,P., Srimanote,P., Tongtawe,P., Khantisitthiporn,O.,
            Supasorn,O. and Thanongsaksrikul,J.
  TITLE     Host neuronal PRSS3 interacts with enterovirus A71 3A protein and
            its role in viral replication
  JOURNAL   Sci Rep 12 (1), 12846 (2022)
   PUBMED   35896602
  REMARK    GeneRIF: Host neuronal PRSS3 interacts with enterovirus A71 3A
            protein and its role in viral replication.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 932)
  AUTHORS   Zhang,Q., Wang,J., Huang,D. and Liu,G.
  TITLE     High Expression of PRSS3 Indicates Unfavorable Clinical Outcomes in
            Colon Adenocarcinoma
  JOURNAL   Appl Immunohistochem Mol Morphol 29 (8), 564-569 (2021)
   PUBMED   33758142
  REMARK    GeneRIF: High Expression of PRSS3 Indicates Unfavorable Clinical
            Outcomes in Colon Adenocarcinoma.
REFERENCE   5  (bases 1 to 932)
  AUTHORS   Aboulouard,S., Wisztorski,M., Duhamel,M., Saudemont,P., Cardon,T.,
            Narducci,F., Lemaire,A.S., Kobeissy,F., Leblanc,E., Fournier,I. and
            Salzet,M.
  TITLE     In-depth proteomics analysis of sentinel lymph nodes from
            individuals with endometrial cancer
  JOURNAL   Cell Rep Med 2 (6), 100318 (2021)
   PUBMED   34195683
  REMARK    GeneRIF: In-depth proteomics analysis of sentinel lymph nodes from
            individuals with endometrial cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 932)
  AUTHORS   Nyaruhucha,C.N., Kito,M. and Fukuoka,S.I.
  TITLE     Identification and expression of the cDNA-encoding human
            mesotrypsin(ogen), an isoform of trypsin with inhibitor resistance
  JOURNAL   J Biol Chem 272 (16), 10573-10578 (1997)
   PUBMED   9099703
REFERENCE   7  (bases 1 to 932)
  AUTHORS   Stubbs,M.T., Huber,R. and Bode,W.
  TITLE     Crystal structures of factor Xa specific inhibitors in complex with
            trypsin: structural grounds for inhibition of factor Xa and
            selectivity against thrombin
  JOURNAL   FEBS Lett 375 (1-2), 103-107 (1995)
   PUBMED   7498454
REFERENCE   8  (bases 1 to 932)
  AUTHORS   Wiegand,U., Corbach,S., Minn,A., Kang,J. and Muller-Hill,B.
  TITLE     Cloning of the cDNA encoding human brain trypsinogen and
            characterization of its product
  JOURNAL   Gene 136 (1-2), 167-175 (1993)
   PUBMED   8294000
REFERENCE   9  (bases 1 to 932)
  AUTHORS   Tani,T., Kawashima,I., Mita,K. and Takiguchi,Y.
  TITLE     Nucleotide sequence of the human pancreatic trypsinogen III cDNA
  JOURNAL   Nucleic Acids Res 18 (6), 1631 (1990)
   PUBMED   2326201
REFERENCE   10 (bases 1 to 932)
  AUTHORS   Rinderknecht,H., Renner,I.G., Abramson,S.B. and Carmack,C.
  TITLE     Mesotrypsin: a new inhibitor-resistant protease from a zymogen in
            human pancreatic tissue and fluid
  JOURNAL   Gastroenterology 86 (4), 681-692 (1984)
   PUBMED   6698368
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356489.14, AL358573.25 and
            AL139113.21.
            
            On Aug 14, 2020 this sequence version replaced NM_001197097.2.
            
            Summary: This gene encodes a trypsinogen, which is a member of the
            trypsin family of serine proteases. This enzyme is expressed in the
            brain and pancreas and is resistant to common trypsin inhibitors.
            It is active on peptide linkages involving the carboxyl group of
            lysine or arginine. This gene is localized to the locus of T cell
            receptor beta variable orphans on chromosome 9. Four transcript
            variants encoding different isoforms have been described for this
            gene. [provided by RefSeq, Oct 2010].
            
            Transcript Variant: This variant (3) contains an alternate in-frame
            exon in the 5' coding region and uses a downstream start codon,
            compared to variant 1. The resulting protein (isoform 3) has a
            shorter N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY052783.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2152568, SAMEA2153932
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AL356489.14        95619-95667
            50-177              AL358573.25        11047-11174
            178-337             AL358573.25        12944-13103
            338-591             AL358573.25        14130-14383
            592-728             AL358573.25        14787-14923
            729-932             AL139113.21        2331-2534
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..932
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /note="serine protease 3"
                     /db_xref="GeneID:5646"
                     /db_xref="HGNC:HGNC:9486"
                     /db_xref="MIM:613578"
     exon            1..49
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /inference="alignment:Splign:2.1.0"
     exon            50..177
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..881
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /EC_number="3.4.21.4"
                     /note="isoform 3 preproprotein is encoded by transcript
                     variant 3; protease, serine, 4 (trypsin 4, brain);
                     mesotrypsinogen; brain trypsinogen; pancreatic trypsinogen
                     III; trypsinogen IV; trypsin-3; protease, serine 3;
                     trypsinogen 4; trypsinogen 5; trypsin IV; trypsin III"
                     /codon_start=1
                     /product="trypsin-3 isoform 3 preproprotein"
                     /protein_id="NP_001184026.3"
                     /db_xref="GeneID:5646"
                     /db_xref="HGNC:HGNC:9486"
                     /db_xref="MIM:613578"
                     /translation="MHMRETSGFTLKKGRSAPLVFHPPDALIAVPFDDDDKIVGGYTC
                     EENSLPYQVSLNSGSHFCGGSLISEQWVVSAAHCYKTRIQVRLGEHNIKVLEGNEQFI
                     NAAKIIRHPKYNRDTLDNDIMLIKLSSPAVINARVSTISLPTTPPAAGTECLISGWGN
                     TLSFGADYPDELKCLDAPVLTQAECKASYPGKITNSMFCVGFLEGGKDSCQRDSGGPV
                     VCNGQLQGVVSWGHGCAWKNRPGVYTKVYNYVDWIKDTIAANS"
     exon            178..337
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /inference="alignment:Splign:2.1.0"
     exon            338..591
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /inference="alignment:Splign:2.1.0"
     exon            592..728
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /inference="alignment:Splign:2.1.0"
     exon            729..932
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      912..917
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /note="hexamer: AATAAA"
     polyA_site      932
                     /gene="PRSS3"
                     /gene_synonym="MTG; PRSS4; T9; TRY3; TRY4"
                     /note="major polyA site"
ORIGIN      
        1 acttggcgag cggcgcggga tgcagacggc tgcgaggcgc tgggcacagg ttgccaggac
       61 aaccgtgagg ctgcataaaa agaacctatg acaggatgca catgagagag acaagtggct
      121 tcacattgaa gaaggggagg agtgcgccat tggttttcca tcctccagat gcactgattg
      181 ctgtcccctt tgacgatgat gacaagattg ttgggggcta cacctgtgag gagaattctc
      241 tcccctacca ggtgtccctg aattctggct cccacttctg cggtggctcc ctcatcagcg
      301 aacagtgggt ggtatcagca gctcactgct acaagacccg catccaggtg agactgggag
      361 agcacaacat caaagtcctg gaggggaatg agcagttcat caatgcggcc aagatcatcc
      421 gccaccctaa atacaacagg gacactctgg acaatgacat catgctgatc aaactctcct
      481 cacctgccgt catcaatgcc cgcgtgtcca ccatctctct gcccaccacc cctccagctg
      541 ctggcactga gtgcctcatc tccggctggg gcaacactct gagctttggt gctgactacc
      601 cagacgagct gaagtgcctg gatgctccgg tgctgaccca ggctgagtgt aaagcctcct
      661 accctggaaa gattaccaac agcatgttct gtgtgggctt ccttgaggga ggcaaggatt
      721 cctgccagcg tgactctggt ggccctgtgg tctgcaacgg acagctccaa ggagttgtct
      781 cctggggcca tggctgtgcc tggaagaaca ggcctggagt ctacaccaag gtctacaact
      841 atgtggactg gattaaggac accatcgctg ccaacagcta aagcccccgg tccctctgca
      901 gtctctatac caataaagtg gccctgctct ca
//
