LOCUS       NM_024081               5543 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens proline rich and Gla domain 4 (PRRG4), mRNA.
ACCESSION   NM_024081 XR_109077
VERSION     NM_024081.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Kliche,J., Garvanska,D.H., Simonetti,L., Badgujar,D.,
            Dobritzsch,D., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Large-scale phosphomimetic screening identifies phospho-modulated
            motif-based protein interactions
  JOURNAL   Mol Syst Biol 19 (7), e11164 (2023)
   PUBMED   37219487
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   3  (bases 1 to 5543)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5543)
  AUTHORS   Zhang,L., Qin,Y., Wu,G., Wang,J., Cao,J., Wang,Y., Wu,D., Yang,K.,
            Zhao,Z., He,L., Lyu,J., Li,H. and Gu,H.
  TITLE     PRRG4 promotes breast cancer metastasis through the recruitment of
            NEDD4 and downregulation of Robo1
  JOURNAL   Oncogene 39 (49), 7196-7208 (2020)
   PUBMED   33037408
  REMARK    GeneRIF: PRRG4 promotes breast cancer metastasis through the
            recruitment of NEDD4 and downregulation of Robo1.
REFERENCE   5  (bases 1 to 5543)
  AUTHORS   Justice,E.D., Barnum,S.J. and Kidd,T.
  TITLE     The WAGR syndrome gene PRRG4 is a functional homologue of the
            commissureless axon guidance gene
  JOURNAL   PLoS Genet 13 (8), e1006865 (2017)
   PUBMED   28859078
  REMARK    GeneRIF: PRRG4 could re-localize hRobo1 from the cell surface,
            suggesting that PRRG4 is a functional homologue of Comm. Comm is
            required for axon guidance and synapse formation in the fly, so
            PRRG4 could contribute to the autistic symptoms of WAGR by
            disturbing either of these processes in the developing human brain.
            Erratum:[PLoS Genet. 2017 Oct 23;13(10):e1007061. doi:
            10.1371/journal.pgen.1007061.. PMID: 29059187]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5543)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   7  (bases 1 to 5543)
  AUTHORS   Yamamoto,T., Togawa,M., Shimada,S., Sangu,N., Shimojima,K. and
            Okamoto,N.
  TITLE     Narrowing of the responsible region for severe developmental delay
            and autistic behaviors in WAGR syndrome down to 1.6 Mb including
            PAX6, WT1, and PRRG4
  JOURNAL   Am J Med Genet A 164A (3), 634-638 (2014)
   PUBMED   24357251
  REMARK    GeneRIF: the unique genotype identified in this study suggested
            that haploinsufficiencies of PAX6 or PRRG4 included in this region
            are candidate genes for severe developmental delay and autistic
            features characteristic of WAGR syndrome.
REFERENCE   8  (bases 1 to 5543)
  AUTHORS   Yazicioglu,M.N., Monaldini,L., Chu,K., Khazi,F.R., Murphy,S.L.,
            Huang,H., Margaritis,P. and High,K.A.
  TITLE     Cellular localization and characterization of cytosolic binding
            partners for Gla domain-containing proteins PRRG4 and PRRG2
  JOURNAL   J Biol Chem 288 (36), 25908-25914 (2013)
   PUBMED   23873930
  REMARK    GeneRIF: Cellular localization and characterization of cytosolic
            binding partners for Gla domain-containing proteins PRRG4 and
            PRRG2.
REFERENCE   9  (bases 1 to 5543)
  AUTHORS   Latourelle,J.C., Pankratz,N., Dumitriu,A., Wilk,J.B., Goldwurm,S.,
            Pezzoli,G., Mariani,C.B., DeStefano,A.L., Halter,C., Gusella,J.F.,
            Nichols,W.C., Myers,R.H. and Foroud,T.
  CONSRTM   PROGENI Investigators, Coordinators and Molecular Genetic
            Laboratories; GenePD Investigators, Coordinators and Molecular
            Genetic Laboratories
  TITLE     Genomewide association study for onset age in Parkinson disease
  JOURNAL   BMC Med Genet 10, 98 (2009)
   PUBMED   19772629
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 5543)
  AUTHORS   Kulman,J.D., Harris,J.E., Xie,L. and Davie,E.W.
  TITLE     Identification of two novel transmembrane gamma-carboxyglutamic
            acid proteins expressed broadly in fetal and adult tissues
  JOURNAL   Proc Natl Acad Sci U S A 98 (4), 1370-1375 (2001)
   PUBMED   11171957
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL035400.13, BC010052.2 and BC063393.1.
            
            On Nov 23, 2018 this sequence version replaced NM_024081.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063393.1, ERR279828.10324.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257836.4/ ENSP00000257836.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AL035400.13        75285-75292         c
            9-802               BC010052.2         1-794
            803-2001            BC063393.1         787-1985
            2002-5543           AL035400.13        47099-50640         c
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p13"
     gene            1..5543
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /note="proline rich and Gla domain 4"
                     /db_xref="GeneID:79056"
                     /db_xref="HGNC:HGNC:30799"
                     /db_xref="MIM:611690"
     exon            1..247
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            248..372
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..950
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /note="transmembrane gamma-carboxyglutamic acid protein 4;
                     proline-rich Gla protein 4; proline rich Gla
                     (G-carboxyglutamic acid) 4 (transmembrane); proline-rich
                     gamma-carboxyglutamic acid protein 4"
                     /codon_start=1
                     /product="transmembrane gamma-carboxyglutamic acid protein
                     4 precursor"
                     /protein_id="NP_076986.1"
                     /db_xref="CCDS:CCDS7881.1"
                     /db_xref="GeneID:79056"
                     /db_xref="HGNC:HGNC:30799"
                     /db_xref="MIM:611690"
                     /translation="MFTLLVLLSQLPTVTLGFPHCARGPKASKHAGEEVFTSKEEANF
                     FIHRRLLYNRFDLELFTPGNLERECNEELCNYEEAREIFVDEDKTIAFWQEYSAKGPT
                     TKSDGNREKIDVMGLLTGLIAAGVFLVIFGLLGYYLCITKCNRLQHPCSSAVYERGRH
                     TPSIIFRRPEEAALSPLPPSVEDAGLPSYEQAVALTRKHSVSPPPPYPGHTKGFRVFK
                     KSMSLPSH"
     exon            373..536
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            537..585
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            586..718
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /inference="alignment:Splign:2.1.0"
     exon            719..5543
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5523..5528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /note="hexamer: AATAAA"
     polyA_site      5543
                     /gene="PRRG4"
                     /gene_synonym="PRGP4; TMG4"
                     /note="major polyA site"
ORIGIN      
        1 ctccgtcggc cgcctccccg gaccgaggca ggacctcacc ccgcgcgtgt tccccgggcg
       61 cccctctgcg aaccccaggc ccttcccagg tttgcgcgcg ggggccatcc agaccctgcg
      121 gagagcgagg cccggagcgt cgccgaggtt tgagggcgcc ggagaccgag ggcctggcgg
      181 ccgaaggaac cgccccaaga agagcctctg gcccgggggc tgctggaaca tgtgcggggg
      241 gacacagttt gtttgacagt tgccagacta tgtttacgct tctggttcta ctcagccaac
      301 tgcccacagt taccctgggg tttcctcatt gcgcaagagg tccaaaggct tctaagcatg
      361 cgggagaaga agtgtttaca tcaaaagaag aagcaaactt tttcatacat agacgccttc
      421 tgtataatag atttgatctg gagctcttca ctcccggcaa cctagaaaga gagtgcaatg
      481 aagaactttg caattatgag gaagccagag agatttttgt ggatgaagat aaaacgattg
      541 cattttggca ggaatattca gctaaaggac caaccacaaa atcagatggc aacagagaga
      601 aaatagatgt tatgggcctt ctgactggat taattgctgc tggagtattt ttggttattt
      661 ttggattact tggctactat ctttgtatca ctaagtgtaa taggctacaa catccatgct
      721 cttcagccgt ctatgaaagg gggaggcaca ctccctccat cattttcaga agacctgagg
      781 aggctgcctt gtctccattg ccgccttctg tggaggatgc aggattacct tcttatgaac
      841 aggcagtggc gctgaccaga aaacacagtg tttcaccacc accaccatat cctgggcaca
      901 caaaaggatt tagggtattt aaaaaatcta tgtctctccc atctcactga ctaccttgtc
      961 attttggtat aagaaatttg tgttatttga taggccgggc atggtggctc atgcctgtaa
     1021 tcccagcact ttgggaggcc aggagttcga gaccagcctg gccaacatgg tgaaacccgg
     1081 tctctactaa aaattcaaaa attacctagg cgtcatgggg catgcctgta gtcccaccta
     1141 cttgggaggc tgaagcagga gaattgctcg aacctgggag gcagaggttg cagtaagctg
     1201 agatcacgcc actgcattcc agcctgggcg acagagcaag actccatctc aaaaataaaa
     1261 taaaaaaaga aagaaagaaa agaagaagaa aagagaagaa ggagaaggag atgaaggagg
     1321 aggaggagga gaaggagaag aagaagaaga agaagaccac aaaagacatg actatccaac
     1381 tttttatgac aaactgcaag gaataaagga agaataagtc catgtactgt accacagaag
     1441 ttctgtctgc atcttggacc tgaacttgat cattatcagc ttgataagag actttttgac
     1501 tctatatcct tgcagttaag aagaaagcac ttttttgtaa tgtttgtttt aatggttcaa
     1561 aaaaaatctt tcttataaag agcataggta gaattagtga actctttgga tcctttgtac
     1621 agataaaggt tatagatttc ttgtgttgaa tattaaaaaa gcaaggatgt ctaaccatta
     1681 agattatcca aagtcaggct gggcgcagtg gctcacgcct gtaatcccag cactttggga
     1741 gggataggtg ggcggatcac ctgaggtcag gagtttgaga ccagcctggc caacatggca
     1801 aaaccccgtc tctacaaaaa tacaaaagaa attagccaga catgatggcg ggtgcctcta
     1861 atcccagcta ctggggaggc tgaggtggga gaatcgcttg aactcgggag gtggaggttg
     1921 tagtgaggcg agattgtgcc attgcactcc aacctgggcg acagagtgag actccatctc
     1981 aaaaaaaaaa aaaaaaaaaa agattatcca aaaagatatt ggacctactc tttcttagga
     2041 tttttttggc ggggggttag aaatacttca cagaatttga catttcagta taaatctgtg
     2101 accttaatat aatcacttgg ttttatatgt taaattattg cacagcagtc atcatatttt
     2161 gcagagttta gttcttaact cttgctgtca gtcatgtttt attataggta gtggggtcag
     2221 tagttttctt cttctaaaaa atactatttg ctatgaagtt agttcttcag aagatacaag
     2281 tttgcaatga aaaggatttg caagggttgt tatgctatca aataaacaga cctaaaatct
     2341 aggagacact agaacttaat gaagttgccc ctgttactga ttagtaaata ctcccatctt
     2401 cgttgcaaaa ttatctctct gtataactac atatgattat tttgaaattt gttaaacttc
     2461 ataagtaata gtttgagaat gtggaaaaag taatttgctt ttctgctctt aaaataatat
     2521 tgattaatgt taccagaaac cttttgcatc tcttaatatg tctgaacaca aaaggaaaac
     2581 accataatca tataaaccca ttctttgtct cttcttctaa atcatatgta taaccagttt
     2641 ttccttaaga atgtagcagc atataagcta taaatttctg gagtatatgc acacaaattt
     2701 atctgggtgg gtatgtaggt atatgtgagt ttgtttcctc ttccaggagc tcaagaaata
     2761 acatactcct gaaaaataga atagaaattt ctgtgcttta tgtttttgcc aggctattac
     2821 attttcttat catttaggat cacttataaa atgatcattg tttattttgt tttataattt
     2881 ttacaggagt gagactgcaa acccacatgt ttgatagttc ctaccaaatt aaaagtttag
     2941 aaagcataaa tcatattaca gtgcatatgt gagtgtatgt ctctctttta agatgcatca
     3001 tcttaaggca gataaaagta agtccttaat cattacagac tgccaaagac tttatccttt
     3061 tagattctca gtctctttgt tgcttgttgc aaataaatca tctagcaaca tttacattta
     3121 attaggaaat ctaacttgct tttaaaagtt acccacgttg catataaaaa tcttgctatt
     3181 ccttgtgtct tggctttaca taagcacttt tgctcatgtg actttgcact ttgcacttat
     3241 tttaatcctc tttaaagggc tacaggcaaa ttctactttg ccataatcac actaaggcat
     3301 ggaagaacaa cttgcccaga atctagcagg ttggtgacaa ctgggtttag aatgagtgag
     3361 cacacttttc tagtcccatg tcatgcctat aaaattacac tgcctcgaat tatgaaattc
     3421 agggatcttg tacataattc taagtttggg acagaaattt acaagcattt ctcatatata
     3481 catacattta tatatgtaca tgttacatat atttagatgt attctcatat acatatgaaa
     3541 atatttatga tgaatagaat tataagatat gtatgtatct tgcactgaat cataatttga
     3601 aatatttcat gaattcattt acttctattg actcccaaaa ttctaactgc aagctagctt
     3661 cagaacctgt gagaacccca ccccacccaa gcagctgcct agatttgtct actgctatca
     3721 ttttgtgtaa agcagttgct ctaacttgaa tgagtctaga attcatcatt aagattgtga
     3781 tatttataga gcatccaatg tggagatcat gatactttaa atataaaaag gaaaatatga
     3841 tagaactgtt attgacaaat gcttataatg attgaaggga aagtatctca acattgtatc
     3901 agtcacatat atttctgttg ctttcaccat tttctattcc tgttgtaagt ttggtggtag
     3961 atttagtttt gtttggttta tctgttttgt ttgtgttttg tctcctggca attgattagt
     4021 gttaatcatt aaatctattt tgacacaagg ttttatatat aattagatca gttttccact
     4081 ttattacaat taagaaatga gtaaaatatg gatataaaag gagaattgga ttgaaaagac
     4141 aaatacacat ttacaaggtc atacctttgg taggacagac aattccatat agaatattct
     4201 cttttgataa tgaaaaaaat taaagagagc acaaactttg actatgtcaa agtattttta
     4261 tttttaattt tttgagacaa ggtcttgctc tgctacccag gctggagtgc agtggtgcaa
     4321 tcacaactca ctgtagcctg aacttcgggg ctcaagcaat cctcccacct cagcctccta
     4381 agtagctggg accacaggtg cacctcggta cacctggcta atttttgtgt tttttgtaga
     4441 gacagggttt caccatgttg cccatgctgg tctctaactc ctgagctcaa ggaatccacc
     4501 cacctcggcc tcccaaagtg ctgggattac aggcatgagc caccacactg gactgctgtg
     4561 tcaaagtttt gcatcttttt tttttttttt tccagaagga gtctcacttt gtcacccagg
     4621 ctggagtgca gtggagtgat catggctcac tgcaacctcc gcctcccagg ttcaaggatt
     4681 tctcctgcct cagcctcctg agtagctgca ttacaggcac ctgccagcac gccttgcaaa
     4741 tttttgtatt tttagtggag atggggtttt gccatgttgg ccaggctggt ctcaaactcc
     4801 tgacctcagg tgatccgccc gcctcagcct cccaaagggc tgggattaca ggcgtgagcc
     4861 actgtgcctg gccccaagtt ttgcatcttt taatgccctc tgaacaaata catagagaaa
     4921 actctcagaa caattaaaac ctgcagagca acagtgtcct ccatgtctta ggtttcaagt
     4981 ttgcctctaa aattctaatc catatttttc tacttctcag ataatttatg tgtgtgtact
     5041 cttcctagac gtacaagaga ctttttaatg ctaaatattt gtcagtgctt aacaaaaact
     5101 caatttcaca ttactcatat tgtttttgtt ttaattgaat gtgaattaaa tttttattag
     5161 ttatttgatt tggaatgtta tgtatgccat taacactatt aggggaatct ctagcatttc
     5221 tgtattttta aagaatttga ttcttttgta gattctgcct gtgtggtcat tttaaaacat
     5281 gtgtgacata tatcagtacc ttcattcttc tatattttgt gtctcctcca acctccaact
     5341 ttttttgttt tttgaaaaat gtttctctaa cacctcaaca gtttaaggta atttagtaca
     5401 catatatcag tatttttgtg atctgaaaaa gcaacccatt ttctaaattc atatttttcc
     5461 taaaacttta ctatgttttt attttaagag gtttcctgtt atatacactt tttacacatg
     5521 caaataaact ttataccaag tga
//
