LOCUS       NM_000951               1403 bp    mRNA    linear   PRI 23-SEP-2022
DEFINITION  Homo sapiens proline rich and Gla domain 2 (PRRG2), transcript
            variant 1, mRNA.
ACCESSION   NM_000951
VERSION     NM_000951.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Benz C, Ali M, Krystkowiak I, Simonetti L, Sayadi A, Mihalic F,
            Kliche J, Andersson E, Jemth P, Davey NE and Ivarsson Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Schuchardt BJ, Mikles DC, Hoang LM, Bhat V, McDonald CB, Sudol M
            and Farooq A.
  TITLE     Ligand binding to WW tandem domains of YAP2 transcriptional
            regulator is under negative cooperativity
  JOURNAL   FEBS J 281 (24), 5532-5551 (2014)
   PUBMED   25283809
REFERENCE   3  (bases 1 to 1403)
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium
  TITLE     Biological insights from 108 schizophrenia-associated genetic loci
  JOURNAL   Nature 511 (7510), 421-427 (2014)
   PUBMED   25056061
REFERENCE   4  (bases 1 to 1403)
  AUTHORS   Yazicioglu MN, Monaldini L, Chu K, Khazi FR, Murphy SL, Huang H,
            Margaritis P and High KA.
  TITLE     Cellular localization and characterization of cytosolic binding
            partners for Gla domain-containing proteins PRRG4 and PRRG2
  JOURNAL   J Biol Chem 288 (36), 25908-25914 (2013)
   PUBMED   23873930
  REMARK    GeneRIF: Cellular localization and characterization of cytosolic
            binding partners for Gla domain-containing proteins PRRG4 and
            PRRG2.
REFERENCE   5  (bases 1 to 1403)
  AUTHORS   Kulman JD, Harris JE, Xie L and Davie EW.
  TITLE     Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent
            protein that binds to the transcriptional coactivator
            Yes-associated protein
  JOURNAL   Proc Natl Acad Sci U S A 104 (21), 8767-8772 (2007)
   PUBMED   17502622
  REMARK    GeneRIF: PRGP2 may be involved in a signal transduction pathway,
            the impairment of which may be an unintended consequence of
            warfarin therapy
REFERENCE   6  (bases 1 to 1403)
  AUTHORS   Fu GK, Wang JT, Yang J, Au-Young J and Stuve LL.
  TITLE     Circular rapid amplification of cDNA ends for high-throughput
            extension cloning of partial genes
  JOURNAL   Genomics 84 (1), 205-210 (2004)
   PUBMED   15203218
REFERENCE   7  (bases 1 to 1403)
  AUTHORS   Jin P, Fu GK, Wilson AD, Yang J, Chien D, Hawkins PR, Au-Young J
            and Stuve LL.
  TITLE     PCR isolation and cloning of novel splice variant mRNAs from known
            drug target genes
  JOURNAL   Genomics 83 (4), 566-571 (2004)
   PUBMED   15028279
REFERENCE   8  (bases 1 to 1403)
  AUTHORS   Kulman JD, Harris JE, Xie L and Davie EW.
  TITLE     Identification of two novel transmembrane gamma-carboxyglutamic
            acid proteins expressed broadly in fetal and adult tissues
  JOURNAL   Proc Natl Acad Sci U S A 98 (4), 1370-1375 (2001)
   PUBMED   11171957
REFERENCE   9  (bases 1 to 1403)
  AUTHORS   Jolliffe CN, Harvey KF, Haines BP, Parasivam G and Kumar S.
  TITLE     Identification of multiple proteins expressed in murine embryos as
            binding partners for the WW domains of the ubiquitin-protein ligase
            Nedd4
  JOURNAL   Biochem J 351 Pt 3, 557-565 (2000)
   PUBMED   11042109
REFERENCE   10 (bases 1 to 1403)
  AUTHORS   Kulman JD, Harris JE, Haldeman BA and Davie EW.
  TITLE     Primary structure and tissue distribution of two novel proline-rich
            gamma-carboxyglutamic acid proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (17), 9058-9062 (1997)
   PUBMED   9256434
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010619.7, CD609900.1,
            AF009243.1 and CB851486.1.
            
            On Nov 22, 2018 this sequence version replaced NM_000951.2.
            
            Summary: The protein encoded by this gene is a single-pass
            transmembrane protein containing an N-terminal
            gamma-carboxyglutamic acid (Gla) domain and tandem
            Pro/Leu-Pro-Xaa-Tyr (PY) motifs at its C-terminal end. The Gla
            domain is exposed on the cell surface while the PY motifs are
            cytoplasmic. The PY motifs of the encoded protein have been shown
            to interact with YAP1, a WW domain-containing protein. Therefore,
            it is thought that the encoded protein may be part of a signal
            transduction pathway. Two transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Oct
            2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279840.2016.1,
                                           SRR1163658.171391.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246794.10/ ENSP00000246794.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC010619.7         168487-168500
            15-170              CD609900.1         8-163
            171-925             AF009243.1         1-755
            926-1403            CB851486.1         23-500              c
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1403
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /note="proline rich and Gla domain 2"
                     /db_xref="GeneID:5639"
                     /db_xref="HGNC:HGNC:9470"
                     /db_xref="MIM:604429"
     exon            1..166
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     exon            167..264
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..788
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; proline-rich Gla (G-carboxglutamic acid)
                     polypeptide 2; proline-rich Gla (G-carboxyglutamic acid)
                     polypeptide 2; transmembrane gamma-carboxyglutamic acid
                     protein 2; proline-rich Gla protein 2; proline-rich
                     gamma-carboxyglutamic acid protein 2; proline rich Gla
                     (G-carboxyglutamic acid) 2"
                     /codon_start=1
                     /product="transmembrane gamma-carboxyglutamic acid protein
                     2 isoform 1 precursor"
                     /protein_id="NP_000942.1"
                     /db_xref="CCDS:CCDS12773.1"
                     /db_xref="GeneID:5639"
                     /db_xref="HGNC:HGNC:9470"
                     /db_xref="MIM:604429"
                     /translation="MRGHPSLLLLYMALTTCLDTSPSEETDQEVFLGPPEAQSFLSSH
                     TRIPRANHWDLELLTPGNLERECLEERCSWEEAREYFEDNTLTERFWESYIYNGKGGR
                     GRVDVASLAVGLTGGILLIVLAGLGAFWYLRWRQHRGQQPCPQEAGLISPLSPLNPLG
                     PPTPLPPPPPPPPGLPTYEQALAASGVHDAPPPPYTSLRRPH"
     exon            265..440
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     exon            441..480
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     exon            481..616
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     exon            617..769
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     exon            770..1403
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1331..1336
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /note="hexamer: AATAAA"
     polyA_site      1363
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /note="major polyA site"
     regulatory      1384..1389
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
                     /note="hexamer: AATAAT"
     polyA_site      1403
                     /gene="PRRG2"
                     /gene_synonym="PRGP2"
ORIGIN      
        1 agttccgccc acctgccaga aacggggatc aggcctggtt accgggagtg gggcgcccct
       61 cctccttatc ccctcccctc ttccctgtcc cctttcacag ctggctgtag ctggccaagg
      121 agttctcgat taaagaggaa ggggcagtgc tcacatttct gggcaggtgt ctggaaaata
      181 tgaggggcca cccctctctg ctgctgctat atatggcatt aaccacctgc ctggatactt
      241 cacccagtga ggagacagac caagaagtct tcctgggtcc cccagaggcc cagagcttcc
      301 tgagtagcca tacccggatt ccaagagcca accactggga cctggagctg ctcacaccag
      361 ggaacctgga acgggagtgt ctggaagaga ggtgttcctg ggaagaggcc agggagtatt
      421 ttgaggacaa cactctcacg gagcgctttt gggagagcta catctacaat ggcaaaggag
      481 ggcgtggacg agtggatgtg gccagcctgg ctgtggggct gacaggtggc atcctgctca
      541 ttgtcctggc cggcctggga gccttttggt atctgcgctg gcgacagcac cgaggccagc
      601 agccctgtcc ccaagaggcc gggctcatta gccctctgag tcctttgaac cctctgggcc
      661 caccgacgcc cctgcctcca cccccacccc cacccccagg cctccccacc tatgagcagg
      721 cgctggcagc ctctggggta cacgacgcac ctccaccccc ctacaccagc ctcaggaggc
      781 ctcactgaag agctgctttc gagacccggc tctccgaacc gtgcccctga ttcataccgg
      841 attccggaag ccgctaggcc tcatagacgc cgaagctgga cttggagtgg ggaatggtgg
      901 gagtaggggt catccggccc gaggcctgcc ctggcacacg cgtttccgcc gcgtatggat
      961 atacacatgt tttcggcaac gtgttcccgt gtcctggccc ctcacgggcc cccacactct
     1021 cctgaccgtg agggcactgg tcagttccgc ccccgtggta ggcagacgcg cggggaaatt
     1081 cggacccagg agcccagccc cggctgtgcc atcttgtgta tgggcagata tgacctgaca
     1141 gccccctcca gtgccacagg gtacgcacac gcagagcccc gcctgtgcac acgcgtgtct
     1201 tcgtgcactc cccgtgcggt acaggggcac ttcgtaaccc agggaaaggg cggggggcat
     1261 atttgcaagc gcgctcggtg cgggcaggct cgcattgcac ccagggagct ggagttgagc
     1321 tgttccccta aataaaaacc cttcggaaag gagaccaaaa aaagcagaaa taatgcaaaa
     1381 aataataatg aaatgaactg cga
//
