LOCUS       NM_181333               1872 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens proline rich 5 (PRR5), transcript variant 1, mRNA.
ACCESSION   NM_181333
VERSION     NM_181333.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1872)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1872)
  AUTHORS   Sundell,G.N., Arnold,R., Ali,M., Naksukpaiboon,P., Orts,J.,
            Guntert,P., Chi,C.N. and Ivarsson,Y.
  TITLE     Proteome-wide analysis of phospho-regulated PDZ domain interactions
  JOURNAL   Mol Syst Biol 14 (8), e8129 (2018)
   PUBMED   30126976
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1872)
  AUTHORS   Chen,X., Liu,M., Tian,Y., Li,J., Qi,Y., Zhao,D., Wu,Z., Huang,M.,
            Wong,C.C.L., Wang,H.W., Wang,J., Yang,H. and Xu,Y.
  TITLE     Cryo-EM structure of human mTOR complex 2
  JOURNAL   Cell Res 28 (5), 518-528 (2018)
   PUBMED   29567957
REFERENCE   6  (bases 1 to 1872)
  AUTHORS   Laplante,M. and Sabatini,D.M.
  TITLE     mTOR signaling in growth control and disease
  JOURNAL   Cell 149 (2), 274-293 (2012)
   PUBMED   22500797
  REMARK    Review article
REFERENCE   7  (bases 1 to 1872)
  AUTHORS   Woo,S.Y., Kim,D.H., Jun,C.B., Kim,Y.M., Haar,E.V., Lee,S.I.,
            Hegg,J.W., Bandhakavi,S., Griffin,T.J. and Kim,D.H.
  TITLE     PRR5, a novel component of mTOR complex 2, regulates
            platelet-derived growth factor receptor beta expression and
            signaling
  JOURNAL   J Biol Chem 282 (35), 25604-25612 (2007)
   PUBMED   17599906
  REMARK    GeneRIF: The inhibition of Akt and S6K1 phosphorylation by PRR5
            knock down correlates with reduction in the expression level of
            platelet-derived growth factor receptor beta (PDGFRbeta).
REFERENCE   8  (bases 1 to 1872)
  AUTHORS   Pearce,L.R., Huang,X., Boudeau,J., Pawlowski,R., Wullschleger,S.,
            Deak,M., Ibrahim,A.F., Gourlay,R., Magnuson,M.A. and Alessi,D.R.
  TITLE     Identification of Protor as a novel Rictor-binding component of
            mTOR complex-2
  JOURNAL   Biochem J 405 (3), 513-522 (2007)
   PUBMED   17461779
  REMARK    GeneRIF: It was demonstrated that immunoprecipitation of Protor-1
            or Protor-2 results in the co-immunoprecipitation of other mTORC2
            subunits, but not Raptor, a specific component of mTORC1.
REFERENCE   9  (bases 1 to 1872)
  AUTHORS   Johnstone,C.N., Castellvi-Bel,S., Chang,L.M., Sung,R.K.,
            Bowser,M.J., Pique,J.M., Castells,A. and Rustgi,A.K.
  TITLE     PRR5 encodes a conserved proline-rich protein predominant in
            kidney: analysis of genomic organization, expression, and mutation
            status in breast and colorectal carcinomas
  JOURNAL   Genomics 85 (3), 338-351 (2005)
   PUBMED   15718101
  REMARK    GeneRIF: mRNA expression analyses revealed PRR5 overexpression in a
            majority of colorectal tumors but substantial downregulation of
            PRR5 expression in a subset of breast tumors and reduced expression
            in two breast cancer cell lines
REFERENCE   10 (bases 1 to 1872)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BK005639.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_181333.3.
            
            Summary: This gene encodes a protein with a proline-rich domain.
            This gene is located in a region of chromosome 22 reported to
            contain a tumor suppressor gene that may be involved in breast and
            colorectal tumorigenesis. The protein is a component of the
            mammalian target of rapamycin complex 2 (mTORC2), and it regulates
            platelet-derived growth factor (PDGF) receptor beta expression and
            PDGF signaling to Akt and S6K1. Alternative splicing and the use of
            alternative promoters results in transcripts encoding different
            isoforms. Read-through transcripts from this gene into the
            downstream Rho GTPase activating protein 8 (ARHGAP8) gene also
            exist, which led to the original description of PRR5 and ARHGAP8
            being a single gene. [provided by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK005639.1, SRR1163658.311420.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336985.11/ ENSP00000337464.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1872              BK005639.1         2-1873
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.31"
     gene            1..1872
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /note="proline rich 5"
                     /db_xref="GeneID:55615"
                     /db_xref="HGNC:HGNC:31682"
                     /db_xref="MIM:609406"
     exon            1..405
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             272..1438
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein observed with Rictor-1; Rho GTPase activating
                     protein 8; proline rich 5 (renal)"
                     /codon_start=1
                     /product="proline-rich protein 5 isoform 1"
                     /protein_id="NP_851850.1"
                     /db_xref="CCDS:CCDS14058.1"
                     /db_xref="GeneID:55615"
                     /db_xref="HGNC:HGNC:31682"
                     /db_xref="MIM:609406"
                     /translation="MRTLRRLKFMSSPSLSDLGKREPAAAADERGTQQRRACANATWN
                     SIHNGVIAVFQRKGLPDQELFSLNEGVRQLLKTELGSFFTEYLQNQLLTKGMVILRDK
                     IRFYEGQKLLDSLAETWDFFFSDVLPMLQAIFYPVQGKEPSVRQLALLHFRNAITLSV
                     KLEDALARAHARVPPAIVQMLLVLQGVHESRGVTEDYLRLETLVQKVVSPYLGTYGLH
                     SSEGPFTHSCILEKRLLRRSRSGDVLAKNPVVRSKSYNTPLLNPVQEHEAEGAAAGGT
                     SIRRHSVSEMTSCPEPQGFSDPPGQGPTGTFRSSPAPHSGPCPSRLYPTTQPPEQGLD
                     PTRSSLPRSSPENLVDQILESVDSDSEGIFIDFGRGRGSGMSDLEGSGGRQSVV"
     exon            406..486
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            487..535
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..593
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            594..685
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            686..826
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            827..962
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1872
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1853..1858
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /note="hexamer: AATAAA"
     polyA_site      1872
                     /gene="PRR5"
                     /gene_synonym="FLJ20185k; PP610; PROTOR-1; PROTOR1"
                     /note="major polyA site"
ORIGIN      
        1 ggcctccgtt tgcgccgggt ctgtgctggc cgcgcgcctg gcgctccacg ctgagcctct
       61 ccgtgcaatg attaacccgg cggggcggcc ggcgcgggac ccgagacgga ggcgcggggc
      121 cggggcggga ccccgcagga ccgctcggct tcctgctctc gccggagttt ccgcgtagag
      181 ggcgcatcgc cggcccgggg cccttggtgc ggcgtggcgc agggcgcggc gtggggcgcg
      241 cgtgggcgcg gcgcaggcgg cccgggtcac catgaggact ctccgcaggt tgaagttcat
      301 gagttcgccc agcctcagtg acctgggcaa gagagagccg gccgccgccg cggacgagcg
      361 gggcacgcag cagcgccggg cctgcgccaa cgccacctgg aacagcatcc acaacggggt
      421 gatcgccgtc ttccagcgca aggggctgcc cgaccaggag ctcttcagcc tcaacgaggg
      481 cgtccggcag ctgttgaaga cagagctggg gtccttcttc acggagtacc tgcagaacca
      541 gctgctgaca aaaggcatgg tgatccttcg ggacaagatt cgcttctatg agggacagaa
      601 gctgctggac tcactggcag agacctggga cttcttcttc agtgacgtgc tgcccatgct
      661 gcaggccatc ttctacccgg tgcagggcaa ggagccatcg gtgcgccagc tggccctgct
      721 gcacttccgg aatgccatca ccctcagtgt gaagctagag gatgcgctgg cccgggccca
      781 tgcccgtgtg ccccctgcca tcgtgcagat gctgctggtg ctgcaggggg tacatgagtc
      841 caggggcgtg actgaggact acctgcgcct ggagacgctg gtccagaagg tggtgtcgcc
      901 atacctgggc acctacggcc tccactccag cgaggggccc ttcacccatt cctgcatcct
      961 ggaaaagcgc ctcctccgcc gctcccgctc gggggacgtg ctggccaaga accctgtggt
     1021 gcgctccaag agctacaaca cgcctctgct gaaccccgtg caggagcacg aggcggaggg
     1081 cgcggcggcc ggcggtacca gcatccgcag gcactctgtg tcggagatga cgtcctgccc
     1141 cgagcctcag ggcttctccg acccgcccgg ccagggcccc accgggacct tcaggtcctc
     1201 cccggcgccc cactcagggc cctgccccag cagactgtac cccacgaccc agccccctga
     1261 gcagggcttg gatcccaccc gcagctccct gccccgctcc agcccggaga acctggtgga
     1321 ccagatcctg gagtccgtgg actcggattc tgaagggatt ttcattgact ttggccgggg
     1381 ccggggctct ggcatgtccg acttggaggg ctctgggggc cggcagagtg tcgtgtgagg
     1441 cctcacagct ggccttgagt ttttactgac acgtccctgt gtgcgggggt gtccatgtgg
     1501 cgtgtgtgtg agtgagactt ttttactgcg tcccgtcccg ccagccctat cggcctcgtc
     1561 actggccttg gtcactttgt atttctgtct tggttggaaa taccatcagc cttccttgct
     1621 cggcccaggt ctgtttcagg catctgagtc ggcgtttacc caggggccgg gccagagacg
     1681 ggggtcggcc gctcgctccc acgctcctcc tgccccagcc ctctggtgtc cacacctgcc
     1741 cacagagaat gtaaacccag tgggctctgc ccacgccggg ccccaaagtg accagactcc
     1801 agcacacctg tctcctcctg cctggggtgg ccatggggat ggaagggggt ggaataaaac
     1861 ctgtcaacct gg
//
